(Linear) (Six-base) MAPSORT of: KIBB4159.seq Check: 7051 from: 1 to: 435 KIBB4159.orf With 182 enzymes: SgfI * July 18, 2007 13:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 435 Size: 133 302 AgeI A'CCGG_T Cuts at: 0 65 435 Size: 65 370 ApoI r'AATT_y Cuts at: 0 349 435 Size: 349 86 BanII G_rGCy'C Cuts at: 0 154 435 Size: 154 281 BbsI GAAGACnn'nnnn_ Cuts at: 0 110 435 Size: 110 325 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 168 435 Size: 168 267 BsaWI w'CCGG_w Cuts at: 0 65 435 Size: 65 370 Bsp1286I G_dGCh'C Cuts at: 0 154 435 Size: 154 281 BsrFI r'CCGG_y Cuts at: 0 65 435 Size: 65 370 BsrGI T'GTAC_A Cuts at: 0 161 435 Size: 161 274 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 435 Size: 133 302 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 193 435 Size: 193 242 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 321 435 Size: 321 114 NspI r_CATG'y Cuts at: 0 314 435 Size: 314 121 SfcI C'TryA_G Cuts at: 0 237 435 Size: 237 198 StyI C'CwwG_G Cuts at: 0 278 435 Size: 278 157 TatI w'GTAC_w Cuts at: 0 161 435 Size: 161 274 Enzymes that do cut: AcuI AgeI ApoI BanII BbsI BplI BsaWI Bsp1286I BsrFI BsrGI Eco57MI FalI MmeI NspI SfcI StyI TatI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI