Restriction map for
pF1KB4165
(Linear) (Six-base) MAPSORT of: KIBB4165.seq Check: 5255 from: 1 to: 2478
KIBB4165.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 843 851 861 1152 1448 2478
Size: 843 8 10 291 296 1030
Fragments arranged by size:
1030 843 296 291 10 8
AatII G_ACGT'C
Cuts at: 0 2120 2478
Size: 2120 358
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 898 2478
Size: 346 552 1580
Fragments arranged by size:
1580 552 346
AfeI AGC'GCT
Cuts at: 0 1719 2478
Size: 1719 759
AflIII A'CryG_T
Cuts at: 0 1511 2478
Size: 1511 967
AhdI GACnn_n'nnGTC
Cuts at: 0 549 2478
Size: 549 1929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 516 604 2086 2478
Size: 516 88 1482 392
Fragments arranged by size:
1482 516 392 88
AlwNI CAG_nnn'CTG
Cuts at: 0 843 876 897 984 1002 1260 1533 2478
Size: 843 33 21 87 18 258 273 945
Fragments arranged by size:
945 843 273 258 87 33 21 18
ApaI G_GGCC'C
Cuts at: 0 75 747 972 1653 2409 2478
Size: 75 672 225 681 756 69
Fragments arranged by size:
756 681 672 225 75 69
ApaLI G'TGCA_C
Cuts at: 0 1069 2478
Size: 1069 1409
AvaI C'yCGr_G
Cuts at: 0 82 153 2478
Size: 82 71 2325
Fragments arranged by size:
2325 82 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 176 209 1620 1653 2218 2251 2478
Size: 176 33 1411 33 565 33 227
Fragments arranged by size:
1411 565 227 176 33 33 33
BamHI G'GATC_C
Cuts at: 0 2446 2478
Size: 2446 32
BanI G'GyrC_C
Cuts at: 0 385 704 1591 2478
Size: 385 319 887 887
Fragments arranged by size:
887 887 385 319
BanII G_rGCy'C
Cuts at: 0 75 83 258 299 747 907 972 1050
Size: 75 8 175 41 448 160 65 78
Cuts at: 1050 1154 1316 1653 2409 2478
Size: 104 162 337 756 69
Fragments arranged by size:
756 448 337 175 162 160 104 78
75 69 65 41 8
BbeI G_GCGC'C
Cuts at: 0 708 1595 2478
Size: 708 887 883
Fragments arranged by size:
887 883 708
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 1476 2478
Size: 628 848 1002
Fragments arranged by size:
1002 848 628
BbvCI CC'TCA_GC
Cuts at: 0 638 814 2478
Size: 638 176 1664
Fragments arranged by size:
1664 638 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 712 746 2478
Size: 712 34 1732
Fragments arranged by size:
1732 712 34
BglI GCCn_nnn'nGGC
Cuts at: 0 124 1589 1802 2478
Size: 124 1465 213 676
Fragments arranged by size:
1465 676 213 124
BglII A'GATC_T
Cuts at: 0 1727 2478
Size: 1727 751
Bme1580I G_kGCm'C
Cuts at: 0 75 219 388 747 972 1073 1653 1798
Size: 75 144 169 359 225 101 580 145
Cuts at: 1798 2156 2409 2478
Size: 358 253 69
Fragments arranged by size:
580 359 358 253 225 169 145 144
101 75 69
BmgBI CAC'GTC
Cuts at: 0 20 1512 2478
Size: 20 1492 966
Fragments arranged by size:
1492 966 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 179 184 279 741 1704 2478
Size: 179 5 95 462 963 774
Fragments arranged by size:
963 774 462 179 95 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1947 2478
Size: 1947 531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 586 982 1006 1801 2478
Size: 195 391 396 24 795 677
Fragments arranged by size:
795 677 396 391 195 24
Bpu10I CC'TnA_GC
Cuts at: 0 259 638 814 2478
Size: 259 379 176 1664
Fragments arranged by size:
1664 379 259 176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1138 2260 2478
Size: 640 498 1122 218
Fragments arranged by size:
1122 640 498 218
BsaI GGTCTCn'nnnn_
Cuts at: 0 718 2478
Size: 718 1760
BsaAI yAC'GTr
Cuts at: 0 2151 2478
Size: 2151 327
BsaHI Gr'CG_yC
Cuts at: 0 705 1088 1592 1687 2117 2386 2478
Size: 705 383 504 95 430 269 92
Fragments arranged by size:
705 504 430 383 269 95 92
BsaWI w'CCGG_w
Cuts at: 0 1091 2453 2478
Size: 1091 1362 25
Fragments arranged by size:
1362 1091 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 775 805 982 1012 1411 1441
Size: 193 30 552 30 177 30 399 30
Cuts at: 1441 1672 1702 2478
Size: 231 30 776
Fragments arranged by size:
776 552 399 231 193 177 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 703 1003 1045 1249 2478
Size: 700 3 300 42 204 1229
Fragments arranged by size:
1229 700 300 204 42 3
BseYI C'CCAG_C
Cuts at: 0 108 114 234 378 533 554 2307 2419
Size: 108 6 120 144 155 21 1753 112
Cuts at: 2419 2478
Size: 59
Fragments arranged by size:
1753 155 144 120 112 108 59 21
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 634 1429 1546 1696 1867 2478
Size: 245 389 795 117 150 171 611
Fragments arranged by size:
795 611 389 245 171 150 117
BsiEI CG_ry'CG
Cuts at: 0 128 630 1904 2478
Size: 128 502 1274 574
Fragments arranged by size:
1274 574 502 128
BsiHKAI G_wGCw'C
Cuts at: 0 518 907 1073 1316 1928 2478
Size: 518 389 166 243 612 550
Fragments arranged by size:
612 550 518 389 243 166
BsiWI C'GTAC_G
Cuts at: 0 1577 2478
Size: 1577 901
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2254 2478
Size: 2254 224
Bsp1286I G_dGCh'C
Cuts at: 0 75 83 219 258 299 388 518 747
Size: 75 8 136 39 41 89 130 229
Cuts at: 747 907 972 1050 1073 1154 1316 1653 1798
Size: 160 65 78 23 81 162 337 145
Cuts at: 1798 1928 2156 2409 2478
Size: 130 228 253 69
Fragments arranged by size:
337 253 229 228 162 160 145 136
130 130 89 81 78 75 69 65
41 39 23 8
BspEI T'CCGG_A
Cuts at: 0 1091 2453 2478
Size: 1091 1362 25
Fragments arranged by size:
1362 1091 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 303 419 484 843 851 861 1152 1448
Size: 303 116 65 359 8 10 291 296
Cuts at: 1448 1760 2189 2478
Size: 312 429 289
Fragments arranged by size:
429 359 312 303 296 291 289 116
65 10 8
BsrBI CCG'CTC
Cuts at: 0 693 966 1937 2478
Size: 693 273 971 541
Fragments arranged by size:
971 693 541 273
BsrDI GCAATG_nn'
Cuts at: 0 728 2120 2478
Size: 728 1392 358
Fragments arranged by size:
1392 728 358
BsrFI r'CCGG_y
Cuts at: 0 148 629 1116 1594 2373 2478
Size: 148 481 487 478 779 105
Fragments arranged by size:
779 487 481 478 148 105
BssSI C'ACGA_G
Cuts at: 0 721 1072 1315 1349 2478
Size: 721 351 243 34 1129
Fragments arranged by size:
1129 721 351 243 34
BstAPI GCAn_nnn'nTGC
Cuts at: 0 447 477 1947 2478
Size: 447 30 1470 531
Fragments arranged by size:
1470 531 447 30
BstEII G'GTnAC_C
Cuts at: 0 1536 2478
Size: 1536 942
BstYI r'GATC_y
Cuts at: 0 1727 1784 2446 2478
Size: 1727 57 662 32
Fragments arranged by size:
1727 662 57 32
Bsu36I CC'TnA_GG
Cuts at: 0 1198 1388 1744 1769 2478
Size: 1198 190 356 25 709
Fragments arranged by size:
1198 709 356 190 25
BtgI C'CryG_G
Cuts at: 0 291 972 1997 2240 2478
Size: 291 681 1025 243 238
Fragments arranged by size:
1025 681 291 243 238
BtsI GCAGTG_nn'
Cuts at: 0 1120 1177 1812 2478
Size: 1120 57 635 666
Fragments arranged by size:
1120 666 635 57
EaeI y'GGCC_r
Cuts at: 0 125 1357 1581 1884 2478
Size: 125 1232 224 303 594
Fragments arranged by size:
1232 594 303 224 125
EagI C'GGCC_G
Cuts at: 0 125 2478
Size: 125 2353
EarI CTCTTCn'nnn_
Cuts at: 0 1001 1669 2478
Size: 1001 668 809
Fragments arranged by size:
1001 809 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 589 2478
Size: 589 1889
EcoICRI GAG'CTC
Cuts at: 0 905 1314 2478
Size: 905 409 1164
Fragments arranged by size:
1164 905 409
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 346 586 898 982 1006 1801 2478
Size: 195 151 240 312 84 24 795 677
Fragments arranged by size:
795 677 312 240 195 151 84 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 12 1006 2074 2185 2478
Size: 12 994 1068 111 293
Fragments arranged by size:
1068 994 293 111 12
EcoO109I rG'GnC_Cy
Cuts at: 0 51 72 408 496 810 1194 1384 1530
Size: 51 21 336 88 314 384 190 146
Cuts at: 1530 1650 1740 1749 2311 2339 2478
Size: 120 90 9 562 28 139
Fragments arranged by size:
562 384 336 314 190 146 139 120
90 88 51 28 21 9
FspI TGC'GCA
Cuts at: 0 481 1267 2478
Size: 481 786 1211
Fragments arranged by size:
1211 786 481
HaeII r_GCGC'y
Cuts at: 0 456 533 708 1595 1721 2478
Size: 456 77 175 887 126 757
Fragments arranged by size:
887 757 456 175 126 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 535 567 775 807 982 1014
Size: 193 32 310 32 208 32 175 32
Cuts at: 1014 1411 1443 1672 1704 2323 2355 2478
Size: 397 32 229 32 619 32 123
Fragments arranged by size:
619 397 310 229 208 193 175 123
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1242 2478
Size: 1242 1236
KasI G'GCGC_C
Cuts at: 0 704 1591 2478
Size: 704 887 887
Fragments arranged by size:
887 887 704
MscI TGG'CCA
Cuts at: 0 1886 2478
Size: 1886 592
MslI CAynn'nnrTG
Cuts at: 0 1339 2478
Size: 1339 1139
MspA1I CmG'CkG
Cuts at: 0 558 564 897 915 1290 1624 1647 1659
Size: 558 6 333 18 375 334 23 12
Cuts at: 1659 1815 2478
Size: 156 663
Fragments arranged by size:
663 558 375 334 333 156 23 18
12 6
NaeI GCC'GGC
Cuts at: 0 1118 1596 2375 2478
Size: 1118 478 779 103
Fragments arranged by size:
1118 779 478 103
NarI GG'CG_CC
Cuts at: 0 705 1592 2478
Size: 705 887 886
Fragments arranged by size:
887 886 705
NcoI C'CATG_G
Cuts at: 0 972 2478
Size: 972 1506
NgoMIV G'CCGG_C
Cuts at: 0 1116 1594 2373 2478
Size: 1116 478 779 105
Fragments arranged by size:
1116 779 478 105
NspI r_CATG'y
Cuts at: 0 591 2478
Size: 591 1887
PfoI T'CCnGG_A
Cuts at: 0 1517 2478
Size: 1517 961
PmlI CAC'GTG
Cuts at: 0 2151 2478
Size: 2151 327
PpuMI rG'GwC_Cy
Cuts at: 0 408 496 1384 1740 1749 2339 2478
Size: 408 88 888 356 9 590 139
Fragments arranged by size:
888 590 408 356 139 88 9
PspOMI G'GGCC_C
Cuts at: 0 71 743 968 1649 2405 2478
Size: 71 672 225 681 756 73
Fragments arranged by size:
756 681 672 225 73 71
PstI C_TGCA'G
Cuts at: 0 314 563 848 902 1190 1289 1658 1981
Size: 314 249 285 54 288 99 369 323
Cuts at: 1981 2189 2468 2478
Size: 208 279 10
Fragments arranged by size:
369 323 314 288 285 279 249 208
99 54 10
PvuII CAG'CTG
Cuts at: 0 558 564 897 915 1290 1659 1815 2478
Size: 558 6 333 18 375 369 156 663
Fragments arranged by size:
663 558 375 369 333 156 18 6
SacI G_AGCT'C
Cuts at: 0 907 1316 2478
Size: 907 409 1162
Fragments arranged by size:
1162 907 409
SapI GCTCTTCn'nnn_
Cuts at: 0 1669 2478
Size: 1669 809
SbfI CC_TGCA'GG
Cuts at: 0 314 848 2478
Size: 314 534 1630
Fragments arranged by size:
1630 534 314
SexAI A'CCwGG_T
Cuts at: 0 2012 2478
Size: 2012 466
SfcI C'TryA_G
Cuts at: 0 310 559 844 898 1186 1285 1654 1977
Size: 310 249 285 54 288 99 369 323
Cuts at: 1977 2185 2464 2478
Size: 208 279 14
Fragments arranged by size:
369 323 310 288 285 279 249 208
99 54 14
SfoI GGC'GCC
Cuts at: 0 706 1593 2478
Size: 706 887 885
Fragments arranged by size:
887 885 706
SmaI CCC'GGG
Cuts at: 0 155 2478
Size: 155 2323
SmlI C'TyrA_G
Cuts at: 0 655 1153 2275 2478
Size: 655 498 1122 203
Fragments arranged by size:
1122 655 498 203
SphI G_CATG'C
Cuts at: 0 591 2478
Size: 591 1887
StuI AGG'CCT
Cuts at: 0 1036 1498 1988 2478
Size: 1036 462 490 490
Fragments arranged by size:
1036 490 490 462
StyI C'CwwG_G
Cuts at: 0 972 2478
Size: 972 1506
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 640 1918 2478
Size: 640 1278 560
Fragments arranged by size:
1278 640 560
TatI w'GTAC_w
Cuts at: 0 1700 2478
Size: 1700 778
Tth111I GACn'n_nGTC
Cuts at: 0 1480 2478
Size: 1480 998
XbaI T'CTAG_A
Cuts at: 0 2361 2478
Size: 2361 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1050 2478
Size: 1050 1428
XmaI C'CCGG_G
Cuts at: 0 153 2478
Size: 153 2325
ZraI GAC'GTC
Cuts at: 0 2118 2478
Size: 2118 360
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PfoI PmlI PpuMI PspOMI PstI PvuII
SacI SapI SbfI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TaqII TatI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AloI ApoI
AscI AseI AsiSI AvrII BciVI BclI BfrBI BlpI
BmtI BsaBI BsmI BspHI BsrGI BssHII BstBI BstXI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EcoRI
EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TstI
XhoI XmnI