Restriction map for
pF1KB4166
(Linear) (Six-base) MAPSORT of: KIBB4166.seq Check: 9882 from: 1 to: 1182
KIBB4166.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 0 23 1182
Size: 23 1159
AflIII A'CryG_T
Cuts at: 0 329 1182
Size: 329 853
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 776 1182
Size: 776 406
AlwNI CAG_nnn'CTG
Cuts at: 0 988 1182
Size: 988 194
ApaI G_GGCC'C
Cuts at: 0 1110 1160 1182
Size: 1110 50 22
Fragments arranged by size:
1110 50 22
AvaI C'yCGr_G
Cuts at: 0 5 1131 1182
Size: 5 1126 51
Fragments arranged by size:
1126 51 5
BanI G'GyrC_C
Cuts at: 0 424 1015 1045 1075 1182
Size: 424 591 30 30 107
Fragments arranged by size:
591 424 107 30 30
BanII G_rGCy'C
Cuts at: 0 1110 1160 1182
Size: 1110 50 22
Fragments arranged by size:
1110 50 22
BbeI G_GCGC'C
Cuts at: 0 428 1182
Size: 428 754
BbvCI CC'TCA_GC
Cuts at: 0 32 905 1182
Size: 32 873 277
Fragments arranged by size:
873 277 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 931 1182
Size: 931 251
BglII A'GATC_T
Cuts at: 0 536 881 1182
Size: 536 345 301
Fragments arranged by size:
536 345 301
Bme1580I G_kGCm'C
Cuts at: 0 760 1048 1078 1110 1160 1182
Size: 760 288 30 32 50 22
Fragments arranged by size:
760 288 50 32 30 22
BmgBI CAC'GTC
Cuts at: 0 135 1182
Size: 135 1047
BmrI ACTGGGnnnn_n'
Cuts at: 0 531 1182
Size: 531 651
BmtI G_CTAG'C
Cuts at: 0 956 1182
Size: 956 226
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 834 1182
Size: 834 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 730 1182
Size: 389 341 452
Fragments arranged by size:
452 389 341
Bpu10I CC'TnA_GC
Cuts at: 0 32 905 1182
Size: 32 873 277
Fragments arranged by size:
873 277 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 1182
Size: 835 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 1179 1182
Size: 55 1124 3
Fragments arranged by size:
1124 55 3
BsaAI yAC'GTr
Cuts at: 0 279 1182
Size: 279 903
BsaHI Gr'CG_yC
Cuts at: 0 284 425 1182
Size: 284 141 757
Fragments arranged by size:
757 284 141
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 838 1182
Size: 838 344
BseYI C'CCAG_C
Cuts at: 0 257 887 1141 1182
Size: 257 630 254 41
Fragments arranged by size:
630 257 254 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 946 1182
Size: 655 291 236
Fragments arranged by size:
655 291 236
Bsp1286I G_dGCh'C
Cuts at: 0 760 1048 1078 1110 1160 1182
Size: 760 288 30 32 50 22
Fragments arranged by size:
760 288 50 32 30 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 677 1182
Size: 220 457 505
Fragments arranged by size:
505 457 220
BsrBI CCG'CTC
Cuts at: 0 1028 1058 1088 1182
Size: 1028 30 30 94
Fragments arranged by size:
1028 94 30 30
BsrFI r'CCGG_y
Cuts at: 0 70 164 698 891 1182
Size: 70 94 534 193 291
Fragments arranged by size:
534 291 193 94 70
BstAPI GCAn_nnn'nTGC
Cuts at: 0 799 1182
Size: 799 383
BstXI CCAn_nnnn'nTGG
Cuts at: 0 586 1167 1182
Size: 586 581 15
Fragments arranged by size:
586 581 15
BstYI r'GATC_y
Cuts at: 0 536 881 1182
Size: 536 345 301
Fragments arranged by size:
536 345 301
BtgI C'CryG_G
Cuts at: 0 341 803 1166 1182
Size: 341 462 363 16
Fragments arranged by size:
462 363 341 16
BtsI GCAGTG_nn'
Cuts at: 0 760 800 1182
Size: 760 40 382
Fragments arranged by size:
760 382 40
DraIII CAC_nnn'GTG
Cuts at: 0 143 645 1182
Size: 143 502 537
Fragments arranged by size:
537 502 143
EaeI y'GGCC_r
Cuts at: 0 291 893 1182
Size: 291 602 289
Fragments arranged by size:
602 291 289
EarI CTCTTCn'nnn_
Cuts at: 0 232 1182
Size: 232 950
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 874 1182
Size: 874 308
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 730 1182
Size: 389 341 452
Fragments arranged by size:
452 389 341
EcoNI CCTnn'n_nnAGG
Cuts at: 0 31 1182
Size: 31 1151
EcoO109I rG'GnC_Cy
Cuts at: 0 474 1106 1182
Size: 474 632 76
Fragments arranged by size:
632 474 76
HaeII r_GCGC'y
Cuts at: 0 428 1154 1182
Size: 428 726 28
Fragments arranged by size:
726 428 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 540 572 1182
Size: 540 32 610
Fragments arranged by size:
610 540 32
KasI G'GCGC_C
Cuts at: 0 424 1182
Size: 424 758
MscI TGG'CCA
Cuts at: 0 293 1182
Size: 293 889
MslI CAynn'nnrTG
Cuts at: 0 196 571 1182
Size: 196 375 611
Fragments arranged by size:
611 375 196
MspA1I CmG'CkG
Cuts at: 0 351 1182
Size: 351 831
NaeI GCC'GGC
Cuts at: 0 700 893 1182
Size: 700 193 289
Fragments arranged by size:
700 289 193
NarI GG'CG_CC
Cuts at: 0 425 1182
Size: 425 757
NcoI C'CATG_G
Cuts at: 0 341 1166 1182
Size: 341 825 16
Fragments arranged by size:
825 341 16
NgoMIV G'CCGG_C
Cuts at: 0 698 891 1182
Size: 698 193 291
Fragments arranged by size:
698 291 193
NheI G'CTAG_C
Cuts at: 0 952 1182
Size: 952 230
NspI r_CATG'y
Cuts at: 0 246 333 945 1182
Size: 246 87 612 237
Fragments arranged by size:
612 246 237 87
PciI A'CATG_T
Cuts at: 0 329 1182
Size: 329 853
PfoI T'CCnGG_A
Cuts at: 0 95 366 1182
Size: 95 271 816
Fragments arranged by size:
816 271 95
PmlI CAC'GTG
Cuts at: 0 279 1182
Size: 279 903
PspOMI G'GGCC_C
Cuts at: 0 1106 1156 1182
Size: 1106 50 26
Fragments arranged by size:
1106 50 26
PstI C_TGCA'G
Cuts at: 0 755 795 1182
Size: 755 40 387
Fragments arranged by size:
755 387 40
PvuII CAG'CTG
Cuts at: 0 351 1182
Size: 351 831
ScaI AGT'ACT
Cuts at: 0 919 1182
Size: 919 263
SfcI C'TryA_G
Cuts at: 0 751 791 1182
Size: 751 40 391
Fragments arranged by size:
751 391 40
SfoI GGC'GCC
Cuts at: 0 426 1182
Size: 426 756
SmlI C'TyrA_G
Cuts at: 0 850 1182
Size: 850 332
StuI AGG'CCT
Cuts at: 0 910 1182
Size: 910 272
StyI C'CwwG_G
Cuts at: 0 155 341 1166 1182
Size: 155 186 825 16
Fragments arranged by size:
825 186 155 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 271 1182
Size: 271 911
TatI w'GTAC_w
Cuts at: 0 917 1182
Size: 917 265
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 348 1182
Size: 348 834
XmnI GAAnn'nnTTC
Cuts at: 0 362 1182
Size: 362 820
Enzymes that do cut:
AccI AflIII AlfI AlwNI ApaI AvaI BanI BanII
BbeI BbvCI BciVI BglII Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraIII EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NspI PciI PfoI
PmlI PspOMI PstI PvuII ScaI SfcI SfoI SmlI
StuI StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbsI BcgI BclI BfrBI BglI
BlpI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NotI NruI NsiI PacI PasI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TstI Tth111I XbaI XhoI XmaI ZraI