Restriction map for
pF1KB4176
(Linear) (Six-base) MAPSORT of: KIBB4176.seq Check: 3240 from: 1 to: 1353
KIBB4176.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 466 1353
Size: 466 887
Acc65I G'GTAC_C
Cuts at: 0 1288 1353
Size: 1288 65
AclI AA'CG_TT
Cuts at: 0 300 1353
Size: 300 1053
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 557 1235 1353
Size: 218 339 678 118
Fragments arranged by size:
678 339 218 118
AhdI GACnn_n'nnGTC
Cuts at: 0 1257 1353
Size: 1257 96
AlwNI CAG_nnn'CTG
Cuts at: 0 36 1108 1353
Size: 36 1072 245
Fragments arranged by size:
1072 245 36
ApoI r'AATT_y
Cuts at: 0 131 307 440 547 903 988 1353
Size: 131 176 133 107 356 85 365
Fragments arranged by size:
365 356 176 133 131 107 85
BanI G'GyrC_C
Cuts at: 0 1288 1353
Size: 1288 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 195 1353
Size: 10 185 1158
Fragments arranged by size:
1158 185 10
BfrBI ATG'CAT
Cuts at: 0 562 604 964 1353
Size: 562 42 360 389
Fragments arranged by size:
562 389 360 42
BmrI ACTGGGnnnn_n'
Cuts at: 0 190 1317 1353
Size: 190 1127 36
Fragments arranged by size:
1127 190 36
BmtI G_CTAG'C
Cuts at: 0 356 1353
Size: 356 997
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1353
Size: 353 1000
BsaAI yAC'GTr
Cuts at: 0 219 1353
Size: 219 1134
BseYI C'CCAG_C
Cuts at: 0 660 1353
Size: 660 693
BsiHKAI G_wGCw'C
Cuts at: 0 900 1353
Size: 900 453
BsmI GAATG_Cn'
Cuts at: 0 499 1353
Size: 499 854
Bsp1286I G_dGCh'C
Cuts at: 0 900 1353
Size: 900 453
BspHI T'CATG_A
Cuts at: 0 785 1353
Size: 785 568
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 665 1353
Size: 665 688
BstAPI GCAn_nnn'nTGC
Cuts at: 0 36 1353
Size: 36 1317
BstBI TT'CG_AA
Cuts at: 0 438 1353
Size: 438 915
BstYI r'GATC_y
Cuts at: 0 1140 1353
Size: 1140 213
BstZ17I GTA'TAC
Cuts at: 0 467 1353
Size: 467 886
BtgI C'CryG_G
Cuts at: 0 935 1353
Size: 935 418
BtsI GCAGTG_nn'
Cuts at: 0 1109 1353
Size: 1109 244
DraI TTT'AAA
Cuts at: 0 305 312 1322 1353
Size: 305 7 1010 31
Fragments arranged by size:
1010 305 31 7
EarI CTCTTCn'nnn_
Cuts at: 0 1033 1353
Size: 1033 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 353 557 1235 1353
Size: 218 135 204 678 118
Fragments arranged by size:
678 218 204 135 118
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1337 1353
Size: 1337 16
EcoO109I rG'GnC_Cy
Cuts at: 0 60 316 1353
Size: 60 256 1037
Fragments arranged by size:
1037 256 60
EcoRI G'AATT_C
Cuts at: 0 131 440 903 1353
Size: 131 309 463 450
Fragments arranged by size:
463 450 309 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1243 1275 1353
Size: 1243 32 78
Fragments arranged by size:
1243 78 32
KpnI G_GTAC'C
Cuts at: 0 1292 1353
Size: 1292 61
MfeI C'AATT_G
Cuts at: 0 263 1353
Size: 263 1090
MslI CAynn'nnrTG
Cuts at: 0 217 1353
Size: 217 1136
MspA1I CmG'CkG
Cuts at: 0 1213 1353
Size: 1213 140
NcoI C'CATG_G
Cuts at: 0 935 1353
Size: 935 418
NheI G'CTAG_C
Cuts at: 0 352 1353
Size: 352 1001
NsiI A_TGCA'T
Cuts at: 0 564 606 966 1353
Size: 564 42 360 387
Fragments arranged by size:
564 387 360 42
NspI r_CATG'y
Cuts at: 0 30 1353
Size: 30 1323
PflMI CCAn_nnn'nTGG
Cuts at: 0 941 1353
Size: 941 412
PpuMI rG'GwC_Cy
Cuts at: 0 60 316 1353
Size: 60 256 1037
Fragments arranged by size:
1037 256 60
PvuII CAG'CTG
Cuts at: 0 1213 1353
Size: 1213 140
SanDI GG'GwC_CC
Cuts at: 0 60 1353
Size: 60 1293
StyI C'CwwG_G
Cuts at: 0 935 1353
Size: 935 418
TatI w'GTAC_w
Cuts at: 0 615 697 1353
Size: 615 82 656
Fragments arranged by size:
656 615 82
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 75 1353
Size: 75 1278
XmnI GAAnn'nnTTC
Cuts at: 0 1228 1353
Size: 1228 125
Enzymes that do cut:
AccI Acc65I AclI AcuI AhdI AlwNI ApoI BanI
BbsI BfrBI BmrI BmtI BpmI BsaAI BseYI BsiHKAI
BsmI Bsp1286I BspHI BspMI BstAPI BstBI BstYI BstZ17I
BtgI BtsI DraI EarI Eco57MI EcoNI EcoO109I EcoRI
Hin4I KpnI MfeI MslI MspA1I NcoI NheI NsiI
NspI PflMI PpuMI PvuII SanDI StyI TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AflIII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI ZraI