Restriction map for
pF1KB4188
(Linear) (Six-base) MAPSORT of: KIBB4188.seq Check: 2314 from: 1 to: 1071
KIBB4188.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 343 1071
Size: 119 224 728
Fragments arranged by size:
728 224 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1004 1071
Size: 1004 67
ApaI G_GGCC'C
Cuts at: 0 1045 1071
Size: 1045 26
ApoI r'AATT_y
Cuts at: 0 331 1022 1071
Size: 331 691 49
Fragments arranged by size:
691 331 49
AvaI C'yCGr_G
Cuts at: 0 569 1071
Size: 569 502
BanII G_rGCy'C
Cuts at: 0 487 1045 1071
Size: 487 558 26
Fragments arranged by size:
558 487 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 1035 1071
Size: 1035 36
BbvCI CC'TCA_GC
Cuts at: 0 449 1071
Size: 449 622
BciVI GTATCCnnnnn_n'
Cuts at: 0 796 1071
Size: 796 275
BglI GCCn_nnn'nGGC
Cuts at: 0 583 1071
Size: 583 488
BglII A'GATC_T
Cuts at: 0 44 1071
Size: 44 1027
Bme1580I G_kGCm'C
Cuts at: 0 1045 1071
Size: 1045 26
BmgBI CAC'GTC
Cuts at: 0 516 1071
Size: 516 555
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1071
Size: 251 820
Bpu10I CC'TnA_GC
Cuts at: 0 449 1071
Size: 449 622
BsaI GGTCTCn'nnnn_
Cuts at: 0 269 1071
Size: 269 802
BsaBI GATnn'nnATC
Cuts at: 0 472 1071
Size: 472 599
BseYI C'CCAG_C
Cuts at: 0 1044 1071
Size: 1044 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 1071
Size: 643 428
BsmI GAATG_Cn'
Cuts at: 0 234 1071
Size: 234 837
Bsp1286I G_dGCh'C
Cuts at: 0 487 1045 1071
Size: 487 558 26
Fragments arranged by size:
558 487 26
BsrBI CCG'CTC
Cuts at: 0 825 1071
Size: 825 246
BsrDI GCAATG_nn'
Cuts at: 0 254 1071
Size: 254 817
BsrFI r'CCGG_y
Cuts at: 0 13 1071
Size: 13 1058
BstBI TT'CG_AA
Cuts at: 0 1026 1071
Size: 1026 45
BstYI r'GATC_y
Cuts at: 0 44 1071
Size: 44 1027
EaeI y'GGCC_r
Cuts at: 0 342 662 1071
Size: 342 320 409
Fragments arranged by size:
409 342 320
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 251 343 1071
Size: 119 132 92 728
Fragments arranged by size:
728 132 119 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 181 1071
Size: 181 890
EcoO109I rG'GnC_Cy
Cuts at: 0 1003 1041 1071
Size: 1003 38 30
Fragments arranged by size:
1003 38 30
EcoRI G'AATT_C
Cuts at: 0 1022 1071
Size: 1022 49
HincII GTy'rAC
Cuts at: 0 929 1071
Size: 929 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 608 1071
Size: 161 447 463
Fragments arranged by size:
463 447 161
MscI TGG'CCA
Cuts at: 0 344 664 1071
Size: 344 320 407
Fragments arranged by size:
407 344 320
MslI CAynn'nnrTG
Cuts at: 0 278 752 1071
Size: 278 474 319
Fragments arranged by size:
474 319 278
MspA1I CmG'CkG
Cuts at: 0 448 1048 1071
Size: 448 600 23
Fragments arranged by size:
600 448 23
NspI r_CATG'y
Cuts at: 0 812 973 1071
Size: 812 161 98
Fragments arranged by size:
812 161 98
PasI CC'CwG_GG
Cuts at: 0 234 1071
Size: 234 837
PpuMI rG'GwC_Cy
Cuts at: 0 1003 1071
Size: 1003 68
PsiI TTA'TAA
Cuts at: 0 655 1071
Size: 655 416
PspOMI G'GGCC_C
Cuts at: 0 1041 1071
Size: 1041 30
PstI C_TGCA'G
Cuts at: 0 447 936 1011 1071
Size: 447 489 75 60
Fragments arranged by size:
489 447 75 60
PvuII CAG'CTG
Cuts at: 0 448 1071
Size: 448 623
SbfI CC_TGCA'GG
Cuts at: 0 1011 1071
Size: 1011 60
SfcI C'TryA_G
Cuts at: 0 443 932 1007 1071
Size: 443 489 75 64
Fragments arranged by size:
489 443 75 64
SmlI C'TyrA_G
Cuts at: 0 569 1071
Size: 569 502
SphI G_CATG'C
Cuts at: 0 973 1071
Size: 973 98
StyI C'CwwG_G
Cuts at: 0 710 1071
Size: 710 361
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 508 1071
Size: 508 563
TatI w'GTAC_w
Cuts at: 0 71 954 1071
Size: 71 883 117
Fragments arranged by size:
883 117 71
XhoI C'TCGA_G
Cuts at: 0 569 1071
Size: 569 502
XmnI GAAnn'nnTTC
Cuts at: 0 578 1071
Size: 578 493
Enzymes that do cut:
AcuI AlfI ApaI ApoI AvaI BanII BbsI BbvCI
BciVI BglI BglII Bme1580I BmgBI BpmI Bpu10I BsaI
BsaBI BseYI BsgI BsmI Bsp1286I BsrBI BsrDI BsrFI
BstBI BstYI EaeI Eco57MI EcoNI EcoO109I EcoRI HincII
MmeI MscI MslI MspA1I NspI PasI PpuMI PsiI
PspOMI PstI PvuII SbfI SfcI SmlI SphI StyI
TaqII TatI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BclI
BfrBI BlpI BmrI BmtI BplI BpuEI BsaAI BsaHI
BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XmaI ZraI