Restriction map for
pF1KB4192
(Linear) (Six-base) MAPSORT of: KIBB4192.seq Check: 2304 from: 1 to: 1479
KIBB4192.orf
With 182 enzymes: SgfI *
January 17, 2012 13:59 ..
AatII G_ACGT'C
Cuts at: 0 689 1479
Size: 689 790
AccI GT'mk_AC
Cuts at: 0 389 545 1479
Size: 389 156 934
Fragments arranged by size:
934 389 156
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1380 1479
Size: 287 1093 99
Fragments arranged by size:
1093 287 99
AflIII A'CryG_T
Cuts at: 0 980 1479
Size: 980 499
AleI CACnn'nnGTG
Cuts at: 0 653 1479
Size: 653 826
AlwNI CAG_nnn'CTG
Cuts at: 0 440 1236 1479
Size: 440 796 243
Fragments arranged by size:
796 440 243
AvaI C'yCGr_G
Cuts at: 0 630 762 1078 1088 1252 1423 1479
Size: 630 132 316 10 164 171 56
Fragments arranged by size:
630 316 171 164 132 56 10
BanI G'GyrC_C
Cuts at: 0 658 1196 1240 1479
Size: 658 538 44 239
Fragments arranged by size:
658 538 239 44
BanII G_rGCy'C
Cuts at: 0 12 1066 1178 1408 1479
Size: 12 1054 112 230 71
Fragments arranged by size:
1054 230 112 71 12
BbeI G_GCGC'C
Cuts at: 0 662 1244 1479
Size: 662 582 235
Fragments arranged by size:
662 582 235
BbsI GAAGACnn'nnnn_
Cuts at: 0 846 1479
Size: 846 633
BciVI GTATCCnnnnn_n'
Cuts at: 0 132 1479
Size: 132 1347
BglI GCCn_nnn'nGGC
Cuts at: 0 1061 1090 1107 1479
Size: 1061 29 17 372
Fragments arranged by size:
1061 372 29 17
Bme1580I G_kGCm'C
Cuts at: 0 749 1479
Size: 749 730
BmgBI CAC'GTC
Cuts at: 0 410 1479
Size: 410 1069
BmrI ACTGGGnnnn_n'
Cuts at: 0 1347 1479
Size: 1347 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 417 1479
Size: 417 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1060 1392 1479
Size: 1060 332 87
Fragments arranged by size:
1060 332 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1479
Size: 142 1337
BsaI GGTCTCn'nnnn_
Cuts at: 0 828 1479
Size: 828 651
BsaBI GATnn'nnATC
Cuts at: 0 229 577 1479
Size: 229 348 902
Fragments arranged by size:
902 348 229
BsaHI Gr'CG_yC
Cuts at: 0 374 659 686 994 1114 1241 1479
Size: 374 285 27 308 120 127 238
Fragments arranged by size:
374 308 285 238 127 120 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 214 1159 1479
Size: 19 195 945 320
Fragments arranged by size:
945 320 195 19
BseYI C'CCAG_C
Cuts at: 0 986 1479
Size: 986 493
BsiHKAI G_wGCw'C
Cuts at: 0 386 758 977 1178 1300 1394 1479
Size: 386 372 219 201 122 94 85
Fragments arranged by size:
386 372 219 201 122 94 85
BsmI GAATG_Cn'
Cuts at: 0 1359 1479
Size: 1359 120
Bsp1286I G_dGCh'C
Cuts at: 0 12 386 749 758 977 1066 1178 1300
Size: 12 374 363 9 219 89 112 122
Cuts at: 1300 1394 1408 1479
Size: 94 14 71
Fragments arranged by size:
374 363 219 122 112 94 89 71
14 12 9
BspHI T'CATG_A
Cuts at: 0 956 1479
Size: 956 523
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 905 1479
Size: 905 574
BsrBI CCG'CTC
Cuts at: 0 500 720 906 1384 1479
Size: 500 220 186 478 95
Fragments arranged by size:
500 478 220 186 95
BsrDI GCAATG_nn'
Cuts at: 0 866 1479
Size: 866 613
BsrFI r'CCGG_y
Cuts at: 0 116 792 802 1199 1318 1479
Size: 116 676 10 397 119 161
Fragments arranged by size:
676 397 161 119 116 10
BssSI C'ACGA_G
Cuts at: 0 310 1177 1299 1479
Size: 310 867 122 180
Fragments arranged by size:
867 310 180 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 440 1479
Size: 440 1039
BstEII G'GTnAC_C
Cuts at: 0 162 453 1479
Size: 162 291 1026
Fragments arranged by size:
1026 291 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 655 1479
Size: 655 824
Bsu36I CC'TnA_GG
Cuts at: 0 134 1479
Size: 134 1345
BtgI C'CryG_G
Cuts at: 0 651 1018 1165 1479
Size: 651 367 147 314
Fragments arranged by size:
651 367 314 147
BtsI GCAGTG_nn'
Cuts at: 0 452 1479
Size: 452 1027
ClaI AT'CG_AT
Cuts at: 0 233 1479
Size: 233 1246
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 933 968 1479
Size: 933 35 511
Fragments arranged by size:
933 511 35
EaeI y'GGCC_r
Cuts at: 0 118 351 422 807 1479
Size: 118 233 71 385 672
Fragments arranged by size:
672 385 233 118 71
EarI CTCTTCn'nnn_
Cuts at: 0 97 515 727 1399 1479
Size: 97 418 212 672 80
Fragments arranged by size:
672 418 212 97 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 631 697 1479
Size: 631 66 782
Fragments arranged by size:
782 631 66
EcoICRI GAG'CTC
Cuts at: 0 1176 1479
Size: 1176 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 1060 1380 1392 1479
Size: 287 773 320 12 87
Fragments arranged by size:
773 320 287 87 12
EcoO109I rG'GnC_Cy
Cuts at: 0 21 1083 1208 1427 1479
Size: 21 1062 125 219 52
Fragments arranged by size:
1062 219 125 52 21
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 288 738 1479
Size: 288 450 741
Fragments arranged by size:
741 450 288
FspI TGC'GCA
Cuts at: 0 444 1345 1479
Size: 444 901 134
Fragments arranged by size:
901 444 134
FspAI rTGC'GCAy
Cuts at: 0 1345 1479
Size: 1345 134
HaeII r_GCGC'y
Cuts at: 0 662 1244 1479
Size: 662 582 235
Fragments arranged by size:
662 582 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1315 1347 1479
Size: 1315 32 132
Fragments arranged by size:
1315 132 32
KasI G'GCGC_C
Cuts at: 0 658 1240 1479
Size: 658 582 239
Fragments arranged by size:
658 582 239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1358 1479
Size: 1358 121
MscI TGG'CCA
Cuts at: 0 353 1479
Size: 353 1126
MslI CAynn'nnrTG
Cuts at: 0 653 742 955 1479
Size: 653 89 213 524
Fragments arranged by size:
653 524 213 89
MspA1I CmG'CkG
Cuts at: 0 990 1020 1262 1479
Size: 990 30 242 217
Fragments arranged by size:
990 242 217 30
NaeI GCC'GGC
Cuts at: 0 118 794 804 1479
Size: 118 676 10 675
Fragments arranged by size:
676 675 118 10
NarI GG'CG_CC
Cuts at: 0 659 1241 1479
Size: 659 582 238
Fragments arranged by size:
659 582 238
NgoMIV G'CCGG_C
Cuts at: 0 116 792 802 1479
Size: 116 676 10 677
Fragments arranged by size:
677 676 116 10
PasI CC'CwG_GG
Cuts at: 0 673 1479
Size: 673 806
PflMI CCAn_nnn'nTGG
Cuts at: 0 1236 1479
Size: 1236 243
PfoI T'CCnGG_A
Cuts at: 0 1037 1479
Size: 1037 442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 514 546 1479
Size: 514 32 933
Fragments arranged by size:
933 514 32
PpuMI rG'GwC_Cy
Cuts at: 0 21 1208 1479
Size: 21 1187 271
Fragments arranged by size:
1187 271 21
PshAI GACnn'nnGTC
Cuts at: 0 685 1479
Size: 685 794
SacI G_AGCT'C
Cuts at: 0 1178 1479
Size: 1178 301
SacII CC_GC'GG
Cuts at: 0 1021 1479
Size: 1021 458
SapI GCTCTTCn'nnn_
Cuts at: 0 727 1399 1479
Size: 727 672 80
Fragments arranged by size:
727 672 80
SfcI C'TryA_G
Cuts at: 0 258 1479
Size: 258 1221
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1090 1479
Size: 1090 389
SfoI GGC'GCC
Cuts at: 0 660 1242 1479
Size: 660 582 237
Fragments arranged by size:
660 582 237
SgrAI Cr'CCGG_yG
Cuts at: 0 1318 1479
Size: 1318 161
SmaI CCC'GGG
Cuts at: 0 632 1090 1479
Size: 632 458 389
Fragments arranged by size:
632 458 389
SmlI C'TyrA_G
Cuts at: 0 157 1479
Size: 157 1322
SspI AAT'ATT
Cuts at: 0 345 1479
Size: 345 1134
StuI AGG'CCT
Cuts at: 0 592 1479
Size: 592 887
StyI C'CwwG_G
Cuts at: 0 1105 1479
Size: 1105 374
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1018 1329 1479
Size: 1018 311 150
Fragments arranged by size:
1018 311 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 997 1029 1479
Size: 997 32 450
Fragments arranged by size:
997 450 32
XmaI C'CCGG_G
Cuts at: 0 630 1088 1479
Size: 630 458 391
Fragments arranged by size:
630 458 391
ZraI GAC'GTC
Cuts at: 0 687 1479
Size: 687 792
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlwNI AvaI BanI
BanII BbeI BbsI BciVI BglI Bme1580I BmgBI BmrI
BplI BpmI BpuEI BsaI BsaBI BsaHI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI Bsu36I BtgI BtsI ClaI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
FspI FspAI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PpiI
PpuMI PshAI SacI SacII SapI SfcI SfiI SfoI
SgrAI SmaI SmlI SspI StuI StyI TaqII TstI
XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BclI BfrBI BglII BlpI
BmtI Bpu10I BsaAI BsaWI BsaXI BsgI BsiEI BsiWI
BsmBI BspEI BsrGI BssHII BstBI BstYI BstZ17I BtgZI
DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI
HincII HindIII HpaI KpnI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PmeI
PmlI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SalI SanDI SbfI ScaI SexAI SgfI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XcmI
XhoI XmnI