Restriction map for
pF1KB4203
(Linear) (Six-base) MAPSORT of: KIBB4203.seq Check: 8684 from: 1 to: 954
KIBB4203.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 741 954
Size: 741 213
AatII G_ACGT'C
Cuts at: 0 665 954
Size: 665 289
AccI GT'mk_AC
Cuts at: 0 266 954
Size: 266 688
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 954
Size: 766 188
AflIII A'CryG_T
Cuts at: 0 317 954
Size: 317 637
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 954
Size: 31 32 891
Fragments arranged by size:
891 32 31
AlwNI CAG_nnn'CTG
Cuts at: 0 744 828 954
Size: 744 84 126
Fragments arranged by size:
744 126 84
ApaI G_GGCC'C
Cuts at: 0 441 954
Size: 441 513
AvaI C'yCGr_G
Cuts at: 0 799 954
Size: 799 155
BamHI G'GATC_C
Cuts at: 0 194 954
Size: 194 760
BanI G'GyrC_C
Cuts at: 0 172 954
Size: 172 782
BanII G_rGCy'C
Cuts at: 0 338 441 954
Size: 338 103 513
Fragments arranged by size:
513 338 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 913 953 954
Size: 913 40 1
Fragments arranged by size:
913 40 1
Bme1580I G_kGCm'C
Cuts at: 0 300 441 954
Size: 300 141 513
Fragments arranged by size:
513 300 141
BmgBI CAC'GTC
Cuts at: 0 318 954
Size: 318 636
BmrI ACTGGGnnnn_n'
Cuts at: 0 130 954
Size: 130 824
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 687 883 954
Size: 463 224 196 71
Fragments arranged by size:
463 224 196 71
BsaBI GATnn'nnATC
Cuts at: 0 697 954
Size: 697 257
BsaHI Gr'CG_yC
Cuts at: 0 662 954
Size: 662 292
BsaWI w'CCGG_w
Cuts at: 0 365 954
Size: 365 589
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 31 61 751 778 781 808 954
Size: 31 30 690 27 3 27 146
Fragments arranged by size:
690 146 31 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 954
Size: 775 179
BseYI C'CCAG_C
Cuts at: 0 773 780 954
Size: 773 7 174
Fragments arranged by size:
773 174 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 954
Size: 436 518
BsiEI CG_ry'CG
Cuts at: 0 266 789 954
Size: 266 523 165
Fragments arranged by size:
523 266 165
BsiHKAI G_wGCw'C
Cuts at: 0 338 878 954
Size: 338 540 76
Fragments arranged by size:
540 338 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 308 954
Size: 308 646
Bsp1286I G_dGCh'C
Cuts at: 0 300 338 441 878 954
Size: 300 38 103 437 76
Fragments arranged by size:
437 300 103 76 38
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 741 954
Size: 741 213
BsrBI CCG'CTC
Cuts at: 0 170 954
Size: 170 784
BsrDI GCAATG_nn'
Cuts at: 0 914 954
Size: 914 40
BsrFI r'CCGG_y
Cuts at: 0 586 954
Size: 586 368
BstEII G'GTnAC_C
Cuts at: 0 105 954
Size: 105 849
BstXI CCAn_nnnn'nTGG
Cuts at: 0 579 954
Size: 579 375
BstYI r'GATC_y
Cuts at: 0 194 323 935 954
Size: 194 129 612 19
Fragments arranged by size:
612 194 129 19
Bsu36I CC'TnA_GG
Cuts at: 0 829 954
Size: 829 125
BtgI C'CryG_G
Cuts at: 0 126 954
Size: 126 828
BtsI GCAGTG_nn'
Cuts at: 0 815 954
Size: 815 139
EaeI y'GGCC_r
Cuts at: 0 182 569 695 954
Size: 182 387 126 259
Fragments arranged by size:
387 259 182 126
EcoICRI GAG'CTC
Cuts at: 0 336 954
Size: 336 618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 687 766 883 954
Size: 463 224 79 117 71
Fragments arranged by size:
463 224 117 79 71
EcoO109I rG'GnC_Cy
Cuts at: 0 438 728 954
Size: 438 290 226
Fragments arranged by size:
438 290 226
FspI TGC'GCA
Cuts at: 0 259 954
Size: 259 695
FspAI rTGC'GCAy
Cuts at: 0 259 954
Size: 259 695
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 751 778 783 810 954
Size: 751 27 5 27 144
Fragments arranged by size:
751 144 27 27 5
HincII GTy'rAC
Cuts at: 0 267 954
Size: 267 687
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 954
Size: 683 271
MscI TGG'CCA
Cuts at: 0 571 697 954
Size: 571 126 257
Fragments arranged by size:
571 257 126
MslI CAynn'nnrTG
Cuts at: 0 178 184 253 479 682 954
Size: 178 6 69 226 203 272
Fragments arranged by size:
272 226 203 178 69 6
MspA1I CmG'CkG
Cuts at: 0 744 954
Size: 744 210
NaeI GCC'GGC
Cuts at: 0 588 954
Size: 588 366
NgoMIV G'CCGG_C
Cuts at: 0 586 954
Size: 586 368
NspI r_CATG'y
Cuts at: 0 258 954
Size: 258 696
PfoI T'CCnGG_A
Cuts at: 0 49 954
Size: 49 905
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 954
Size: 31 32 891
Fragments arranged by size:
891 32 31
PpuMI rG'GwC_Cy
Cuts at: 0 728 954
Size: 728 226
PspOMI G'GGCC_C
Cuts at: 0 437 954
Size: 437 517
PvuII CAG'CTG
Cuts at: 0 744 954
Size: 744 210
SacI G_AGCT'C
Cuts at: 0 338 954
Size: 338 616
SalI G'TCGA_C
Cuts at: 0 265 954
Size: 265 689
SanDI GG'GwC_CC
Cuts at: 0 728 954
Size: 728 226
StyI C'CwwG_G
Cuts at: 0 350 408 954
Size: 350 58 546
Fragments arranged by size:
546 350 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 33 954
Size: 33 921
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 117 690 954
Size: 117 573 264
Fragments arranged by size:
573 264 117
ZraI GAC'GTC
Cuts at: 0 663 954
Size: 663 291
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AloI AlwNI ApaI
AvaI BamHI BanI BanII BbsI Bme1580I BmgBI BmrI
BpmI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EcoICRI
Eco57MI EcoO109I FspI FspAI Hin4I HincII MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PfoI PpiI PpuMI
PspOMI PvuII SacI SalI SanDI StyI TaqII XcmI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsiWI BsmI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoNI EcoRI EcoRV FalI FseI HaeII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI