Restriction map for pF1KB4216
 (Linear) (Six-base) MAPSORT of: KIBB4216.seq  Check: 361  from: 1  to: 3423



KIBB4216.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     594    3278    3423 
   Size:        594    2684     145

  Fragments arranged by size: 

                2684     594     145
 
AatII G_ACGT'C

Cuts at:      0    2635    3423 
   Size:       2635     788
 
AccI GT'mk_AC

Cuts at:      0    1430    3423 
   Size:       1430    1993
 
Acc65I G'GTAC_C

Cuts at:      0     945    3423 
   Size:        945    2478
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     242     376     526     793    1201    1276    3423 
   Size:        242     134     150     267     408      75    2147

  Fragments arranged by size: 

                2147     408     267     242     150     134      75
 
AfeI AGC'GCT

Cuts at:      0    2954    3423 
   Size:       2954     469
 
AflIII A'CryG_T

Cuts at:      0    3005    3423 
   Size:       3005     418
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1314    3423 
   Size:       1314    2109
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     683     707    2434    2794    3423 
   Size:        683      24    1727     360     629

  Fragments arranged by size: 

                1727     683     629     360      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0     694    1306    1549    1582    1777    1951    2235    3016 
   Size:        694     612     243      33     195     174     284     781

Cuts at:   3016    3159    3423 
   Size:        143     264

  Fragments arranged by size: 

                 781     694     612     284     264     243     195     174
                 143      33
 
ApaI G_GGCC'C

Cuts at:      0    1518    3423 
   Size:       1518    1905
 
ApoI r'AATT_y

Cuts at:      0    2497    3423 
   Size:       2497     926
 
AvaI C'yCGr_G

Cuts at:      0    1988    2245    3294    3423 
   Size:       1988     257    1049     129

  Fragments arranged by size: 

                1988    1049     257     129
 
AvrII C'CTAG_G

Cuts at:      0    1224    3423 
   Size:       1224    2199
 
BamHI G'GATC_C

Cuts at:      0    2053    3423 
   Size:       2053    1370
 
BanI G'GyrC_C

Cuts at:      0     945    1727    3423 
   Size:        945     782    1696

  Fragments arranged by size: 

                1696     945     782
 
BanII G_rGCy'C

Cuts at:      0     974    1518    2171    2229    2566    2584    2743    3423 
   Size:        974     544     653      58     337      18     159     680

  Fragments arranged by size: 

                 974     680     653     544     337     159      58      18
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     781    1914    1973    3423 
   Size:        781    1133      59    1450

  Fragments arranged by size: 

                1450    1133     781      59
 
BbvCI CC'TCA_GC

Cuts at:      0    2218    3423 
   Size:       2218    1205
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2236    2270    3423 
   Size:       2236      34    1153

  Fragments arranged by size: 

                2236    1153      34
 
BclI T'GATC_A

Cuts at:      0     452    3423 
   Size:        452    2971
 
BfrBI ATG'CAT

Cuts at:      0    2781    3423 
   Size:       2781     642
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1954    2240    2983    3423 
   Size:       1954     286     743     440

  Fragments arranged by size: 

                1954     743     440     286
 
BglII A'GATC_T

Cuts at:      0    1743    3423 
   Size:       1743    1680
 
Bme1580I G_kGCm'C

Cuts at:      0     935    1518    1821    2364    3423 
   Size:        935     583     303     543    1059

  Fragments arranged by size: 

                1059     935     583     543     303
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1419    3423 
   Size:       1419    2004
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49    1743    2443    2487    2492    2533    2559    2647 
   Size:         49    1694     700      44       5      41      26      88

Cuts at:   2647    3358    3423 
   Size:        711      65

  Fragments arranged by size: 

                1694     711     700      88      65      49      44      41
                  26       5
 
Bpu10I CC'TnA_GC

Cuts at:      0    1648    2218    3423 
   Size:       1648     570    1205

  Fragments arranged by size: 

                1648    1205     570
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     533     988    1897    2155    2575    3423 
   Size:        533     455     909     258     420     848

  Fragments arranged by size: 

                 909     848     533     455     420     258
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      25      34    2468    2989    3423 
   Size:         25       9    2434     521     434

  Fragments arranged by size: 

                2434     521     434      25       9
 
BsaAI yAC'GTr

Cuts at:      0    2907    3423 
   Size:       2907     516
 
BsaBI GATnn'nnATC

Cuts at:      0    1748    3423 
   Size:       1748    1675
 
BsaHI Gr'CG_yC

Cuts at:      0    1082    2632    3423 
   Size:       1082    1550     791

  Fragments arranged by size: 

                1550    1082     791
 
BsaWI w'CCGG_w

Cuts at:      0    1730    3423 
   Size:       1730    1693
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1690    1720    3343    3373    3423 
   Size:       1690      30    1623      30      50

  Fragments arranged by size: 

                1690    1623      50      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1020    1397    1698    1775    2128    3279    3423 
   Size:       1020     377     301      77     353    1151     144

  Fragments arranged by size: 

                1151    1020     377     353     301     144      77
 
BseYI C'CCAG_C

Cuts at:      0      18      54     404     410     623    1299    1419    2193 
   Size:         18      36     350       6     213     676     120     774

Cuts at:   2193    2373    3078    3393    3423 
   Size:        180     705     315      30

  Fragments arranged by size: 

                 774     705     676     350     315     213     180     120
                  36      30      18       6
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     628     703     727    2002    2011    2129    3423 
   Size:        628      75      24    1275       9     118    1294

  Fragments arranged by size: 

                1294    1275     628     118      75      24       9
 
BsiEI CG_ry'CG

Cuts at:      0    3354    3423 
   Size:       3354      69
 
BsiHKAI G_wGCw'C

Cuts at:      0    2134    2252    2511    2584    2591    3196    3423 
   Size:       2134     118     259      73       7     605     227

  Fragments arranged by size: 

                2134     605     259     227     118      73       7
 
BsmI GAATG_Cn'

Cuts at:      0     522    3423 
   Size:        522    2901
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2663    3423 
   Size:       2663     760
 
Bsp1286I G_dGCh'C

Cuts at:      0     935     974    1518    1821    2134    2171    2229    2252 
   Size:        935      39     544     303     313      37      58      23

Cuts at:   2252    2364    2511    2566    2584    2591    2743    3196    3423 
   Size:        112     147      55      18       7     152     453     227

  Fragments arranged by size: 

                 935     544     453     313     303     227     152     147
                 112      58      55      39      37      23      18       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     594     702     933    1134    1804    2382    2426    2939 
   Size:        594     108     231     201     670     578      44     513

Cuts at:   2939    3278    3423 
   Size:        339     145

  Fragments arranged by size: 

                 670     594     578     513     339     231     201     145
                 108      44
 
BsrBI CCG'CTC

Cuts at:      0    1436    2316    3237    3423 
   Size:       1436     880     921     186

  Fragments arranged by size: 

                1436     921     880     186
 
BsrDI GCAATG_nn'

Cuts at:      0     228    2384    2829    3410    3423 
   Size:        228    2156     445     581      13

  Fragments arranged by size: 

                2156     581     445     228      13
 
BsrFI r'CCGG_y

Cuts at:      0     585    1780    1931    2012    3353    3423 
   Size:        585    1195     151      81    1341      70

  Fragments arranged by size: 

                1341    1195     585     151      81      70
 
BsrGI T'GTAC_A

Cuts at:      0    2070    3423 
   Size:       2070    1353
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     606    1777    2025    3371    3423 
   Size:        606    1171     248    1346      52

  Fragments arranged by size: 

                1346    1171     606     248      52
 
BstEII G'GTnAC_C

Cuts at:      0     588    1713    3423 
   Size:        588    1125    1710

  Fragments arranged by size: 

                1710    1125     588
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2511    2724    3255    3423 
   Size:       2511     213     531     168

  Fragments arranged by size: 

                2511     531     213     168
 
BstYI r'GATC_y

Cuts at:      0    1743    1749    2053    2120    2144    3423 
   Size:       1743       6     304      67      24    1279

  Fragments arranged by size: 

                1743    1279     304      67      24       6
 
Bsu36I CC'TnA_GG

Cuts at:      0    2453    3423 
   Size:       2453     970
 
BtgI C'CryG_G

Cuts at:      0      35    2162    3423 
   Size:         35    2127    1261

  Fragments arranged by size: 

                2127    1261      35
 
BtsI GCAGTG_nn'

Cuts at:      0     174     204     888    1510    1563    1819    3314    3404 
   Size:        174      30     684     622      53     256    1495      90

Cuts at:   3404    3423 
   Size:         19

  Fragments arranged by size: 

                1495     684     622     256     174      90      53      30
                  19
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     854     889    2627    2662    2856    2891    3423 
   Size:        854      35    1738      35     194      35     532

  Fragments arranged by size: 

                1738     854     532     194      35      35      35
 
EaeI y'GGCC_r

Cuts at:      0     644    1551    1693    2204    2648    2847    3225    3317 
   Size:        644     907     142     511     444     199     378      92

Cuts at:   3317    3423 
   Size:        106

  Fragments arranged by size: 

                 907     644     511     444     378     199     142     106
                  92
 
EarI CTCTTCn'nnn_

Cuts at:      0    1619    2480    3423 
   Size:       1619     861     943

  Fragments arranged by size: 

                1619     943     861
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1008    2046    2586    3064    3423 
   Size:       1008    1038     540     478     359

  Fragments arranged by size: 

                1038    1008     540     478     359
 
EcoICRI GAG'CTC

Cuts at:      0    2582    3423 
   Size:       2582     841
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     242     376     526     793    1201    1276    1743 
   Size:         49     193     134     150     267     408      75     467

Cuts at:   1743    2443    2487    2492    2533    2559    2647    3358    3423 
   Size:        700      44       5      41      26      88     711      65

  Fragments arranged by size: 

                 711     700     467     408     267     193     150     134
                  88      75      65      49      44      41      26       5
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1722    2749    3340    3423 
   Size:       1722    1027     591      83

  Fragments arranged by size: 

                1722    1027     591      83
 
EcoO109I rG'GnC_Cy

Cuts at:      0     714    1316    1514    2721    3039    3423 
   Size:        714     602     198    1207     318     384

  Fragments arranged by size: 

                1207     714     602     384     318     198
 
EcoRI G'AATT_C

Cuts at:      0    2497    3423 
   Size:       2497     926
 
EcoRV GAT'ATC

Cuts at:      0    1024    3423 
   Size:       1024    2399
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1938    2214    2775    3423 
   Size:       1938     276     561     648

  Fragments arranged by size: 

                1938     648     561     276
 
FspI TGC'GCA

Cuts at:      0    2390    3055    3423 
   Size:       2390     665     368

  Fragments arranged by size: 

                2390     665     368
 
HaeII r_GCGC'y

Cuts at:      0    1420    1988    2956    3017    3360    3423 
   Size:       1420     568     968      61     343      63

  Fragments arranged by size: 

                1420     968     568     343      63      61
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1300    1332    1596    1628    3343    3375    3423 
   Size:       1300      32     264      32    1715      32      48

  Fragments arranged by size: 

                1715    1300     264      48      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1204    2041    3423 
   Size:       1204     837    1382

  Fragments arranged by size: 

                1382    1204     837
 
KpnI G_GTAC'C

Cuts at:      0     949    3423 
   Size:        949    2474
 
MluI A'CGCG_T

Cuts at:      0    3005    3423 
   Size:       3005     418
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     352    3057    3423 
   Size:        352    2705     366

  Fragments arranged by size: 

                2705     366     352
 
MscI TGG'CCA

Cuts at:      0    2206    2650    2849    3423 
   Size:       2206     444     199     574

  Fragments arranged by size: 

                2206     574     444     199
 
MspA1I CmG'CkG

Cuts at:      0      11     147     191     242     432     627    1774    2025 
   Size:         11     136      44      51     190     195    1147     251

Cuts at:   2025    2338    2377    2657    2680    2916    3156    3303    3423 
   Size:        313      39     280      23     236     240     147     120

  Fragments arranged by size: 

                1147     313     280     251     240     236     195     190
                 147     136     120      51      44      39      23      11
            
 
NaeI GCC'GGC

Cuts at:      0    1782    1933    3423 
   Size:       1782     151    1490

  Fragments arranged by size: 

                1782    1490     151
 
NcoI C'CATG_G

Cuts at:      0      35    2162    3423 
   Size:         35    2127    1261

  Fragments arranged by size: 

                2127    1261      35
 
NgoMIV G'CCGG_C

Cuts at:      0    1780    1931    3423 
   Size:       1780     151    1492

  Fragments arranged by size: 

                1780    1492     151
 
NsiI A_TGCA'T

Cuts at:      0    2783    3423 
   Size:       2783     640
 
NspI r_CATG'y

Cuts at:      0    2785    3423 
   Size:       2785     638
 
PasI CC'CwG_GG

Cuts at:      0      72     374    2292    2601    2794    3423 
   Size:         72     302    1918     309     193     629

  Fragments arranged by size: 

                1918     629     309     302     193      72
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     561    2292    2657    2988    3159    3423 
   Size:        561    1731     365     331     171     264

  Fragments arranged by size: 

                1731     561     365     331     264     171
 
PfoI T'CCnGG_A

Cuts at:      0    1858    2575    3423 
   Size:       1858     717     848

  Fragments arranged by size: 

                1858     848     717
 
PmlI CAC'GTG

Cuts at:      0    2907    3423 
   Size:       2907     516
 
PpuMI rG'GwC_Cy

Cuts at:      0     714    1316    3039    3423 
   Size:        714     602    1723     384

  Fragments arranged by size: 

                1723     714     602     384
 
PspOMI G'GGCC_C

Cuts at:      0    1514    3423 
   Size:       1514    1909
 
PstI C_TGCA'G

Cuts at:      0     662    1505    1852    1886    2052    2773    3423 
   Size:        662     843     347      34     166     721     650

  Fragments arranged by size: 

                 843     721     662     650     347     166      34
 
PvuII CAG'CTG

Cuts at:      0     147     432    1774    2025    2338    2377    2657    2680 
   Size:        147     285    1342     251     313      39     280      23

Cuts at:   2680    2916    3156    3423 
   Size:        236     240     267

  Fragments arranged by size: 

                1342     313     285     280     267     251     240     236
                 147      39      23
 
SacI G_AGCT'C

Cuts at:      0    2584    3423 
   Size:       2584     839
 
SanDI GG'GwC_CC

Cuts at:      0    1316    3423 
   Size:       1316    2107
 
SbfI CC_TGCA'GG

Cuts at:      0    2052    3423 
   Size:       2052    1371
 
ScaI AGT'ACT

Cuts at:      0    2518    3423 
   Size:       2518     905
 
SexAI A'CCwGG_T

Cuts at:      0    1064    3423 
   Size:       1064    2359
 
SfcI C'TryA_G

Cuts at:      0     658    1501    1848    1875    1882    2048    2432    2769 
   Size:        658     843     347      27       7     166     384     337

Cuts at:   2769    3423 
   Size:        654

  Fragments arranged by size: 

                 843     658     654     384     347     337     166      27
                   7
 
SmaI CCC'GGG

Cuts at:      0    1990    3296    3423 
   Size:       1990    1306     127

  Fragments arranged by size: 

                1990    1306     127
 
SmlI C'TyrA_G

Cuts at:      0     548     967    1912    2170    2590    3423 
   Size:        548     419     945     258     420     833

  Fragments arranged by size: 

                 945     833     548     420     419     258
 
SphI G_CATG'C

Cuts at:      0    2785    3423 
   Size:       2785     638
 
StuI AGG'CCT

Cuts at:      0    2404    3423 
   Size:       2404    1019
 
StyI C'CwwG_G

Cuts at:      0      35    1224    2162    2342    2441    2717    3423 
   Size:         35    1189     938     180      99     276     706

  Fragments arranged by size: 

                1189     938     706     276     180      99      35
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      10     292     387    1550    1701    3163    3385    3423 
   Size:         10     282      95    1163     151    1462     222      38

  Fragments arranged by size: 

                1462    1163     282     222     151      95      38      10
            
 
TatI w'GTAC_w

Cuts at:      0     615    2070    2516    3423 
   Size:        615    1455     446     907

  Fragments arranged by size: 

                1455     907     615     446
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     345     377    1690    1722    3056    3088    3423 
   Size:        345      32    1313      32    1334      32     335

  Fragments arranged by size: 

                1334    1313     345     335      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1066    3423 
   Size:       1066    2357
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1833    2236    2985    3423 
   Size:       1833     403     749     438

  Fragments arranged by size: 

                1833     749     438     403
 
XmaI C'CCGG_G

Cuts at:      0    1988    3294    3423 
   Size:       1988    1306     129

  Fragments arranged by size: 

                1988    1306     129
 
XmnI GAAnn'nnTTC

Cuts at:      0    1921    2305    3423 
   Size:       1921     384    1118

  Fragments arranged by size: 

                1921    1118     384
 
ZraI GAC'GTC

Cuts at:      0    2633    3423 
   Size:       2633     790
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI
     AlfI    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI
    BanII     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI    CspCI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I  HindIII     KpnI     MluI
     MmeI     MscI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI
     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SbfI     ScaI    SexAI     SfcI     SmaI     SmlI
     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XcmI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AleI     AloI    ApaLI     AscI     AseI
    AsiSI     BaeI     BbeI    BciVI     BlpI    BmgBI     BmrI     BmtI
    BsiWI    BspEI    BspHI   BssHII    BssSI    BstBI  BstZ17I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     FseI    FspAI   HincII
     HpaI     KasI     MfeI     MslI     NarI     NdeI     NheI     NotI
     NruI     PacI     PciI     PmeI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SspI     SwaI     XbaI
     XhoI
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