Restriction map for
pF1KB4218
(Linear) (Six-base) MAPSORT of: KIBB4218.seq Check: 2348 from: 1 to: 2394
KIBB4218.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1499 2394
Size: 1499 895
AatII G_ACGT'C
Cuts at: 0 1331 2394
Size: 1331 1063
AccI GT'mk_AC
Cuts at: 0 2063 2394
Size: 2063 331
Acc65I G'GTAC_C
Cuts at: 0 433 2394
Size: 433 1961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2320 2394
Size: 2320 74
AhdI GACnn_n'nnGTC
Cuts at: 0 1608 2394
Size: 1608 786
AleI CACnn'nnGTG
Cuts at: 0 640 2394
Size: 640 1754
AlwNI CAG_nnn'CTG
Cuts at: 0 82 2394
Size: 82 2312
ApoI r'AATT_y
Cuts at: 0 246 542 1064 2293 2394
Size: 246 296 522 1229 101
Fragments arranged by size:
1229 522 296 246 101
AseI AT'TA_AT
Cuts at: 0 881 2394
Size: 881 1513
AvaI C'yCGr_G
Cuts at: 0 71 191 754 1730 1785 1834 2182 2394
Size: 71 120 563 976 55 49 348 212
Fragments arranged by size:
976 563 348 212 120 71 55 49
AvrII C'CTAG_G
Cuts at: 0 153 2394
Size: 153 2241
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 425 458 2394
Size: 425 33 1936
Fragments arranged by size:
1936 425 33
BamHI G'GATC_C
Cuts at: 0 652 2394
Size: 652 1742
BanI G'GyrC_C
Cuts at: 0 433 1441 1487 1594 1725 2178 2221 2394
Size: 433 1008 46 107 131 453 43 173
Fragments arranged by size:
1008 453 433 173 131 107 46 43
BanII G_rGCy'C
Cuts at: 0 302 1835 2394
Size: 302 1533 559
Fragments arranged by size:
1533 559 302
BbeI G_GCGC'C
Cuts at: 0 1598 2394
Size: 1598 796
BbsI GAAGACnn'nnnn_
Cuts at: 0 170 2394
Size: 170 2224
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2066 2100 2394
Size: 2066 34 294
Fragments arranged by size:
2066 294 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1117 2394
Size: 1117 1277
BclI T'GATC_A
Cuts at: 0 381 2394
Size: 381 2013
BfrBI ATG'CAT
Cuts at: 0 850 2394
Size: 850 1544
BlpI GC'TnA_GC
Cuts at: 0 1888 2394
Size: 1888 506
Bme1580I G_kGCm'C
Cuts at: 0 18 1730 2183 2394
Size: 18 1712 453 211
Fragments arranged by size:
1712 453 211 18
BmgBI CAC'GTC
Cuts at: 0 2214 2394
Size: 2214 180
BmrI ACTGGGnnnn_n'
Cuts at: 0 251 2394
Size: 251 2143
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 34 1095 2394
Size: 34 1061 1299
Fragments arranged by size:
1299 1061 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 2394
Size: 1145 1249
Bpu10I CC'TnA_GC
Cuts at: 0 797 2394
Size: 797 1597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 1804 2394
Size: 455 1349 590
Fragments arranged by size:
1349 590 455
BsaI GGTCTCn'nnnn_
Cuts at: 0 1078 1515 2394
Size: 1078 437 879
Fragments arranged by size:
1078 879 437
BsaAI yAC'GTr
Cuts at: 0 184 1977 2394
Size: 184 1793 417
Fragments arranged by size:
1793 417 184
BsaHI Gr'CG_yC
Cuts at: 0 1328 1595 1658 2394
Size: 1328 267 63 736
Fragments arranged by size:
1328 736 267 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 160 190 1567 1597 1981 2011 2394
Size: 160 30 1377 30 384 30 383
Fragments arranged by size:
1377 384 383 160 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 184 1940 2160 2394
Size: 184 1756 220 234
Fragments arranged by size:
1756 234 220 184
BseYI C'CCAG_C
Cuts at: 0 60 159 2000 2394
Size: 60 99 1841 394
Fragments arranged by size:
1841 394 99 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1387 1698 1957 2160 2394
Size: 74 1313 311 259 203 234
Fragments arranged by size:
1313 311 259 234 203 74
BsiEI CG_ry'CG
Cuts at: 0 1739 2394
Size: 1739 655
BsiHKAI G_wGCw'C
Cuts at: 0 471 1982 2117 2394
Size: 471 1511 135 277
Fragments arranged by size:
1511 471 277 135
BsmI GAATG_Cn'
Cuts at: 0 1080 2394
Size: 1080 1314
BsmBI CGTCTCn'nnnn_
Cuts at: 0 269 2394
Size: 269 2125
Bsp1286I G_dGCh'C
Cuts at: 0 18 302 471 1730 1835 1982 2117 2183
Size: 18 284 169 1259 105 147 135 66
Cuts at: 2183 2394
Size: 211
Fragments arranged by size:
1259 284 211 169 147 135 105 66
18
BspHI T'CATG_A
Cuts at: 0 2328 2394
Size: 2328 66
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 67 1499 2018 2394
Size: 67 1432 519 376
Fragments arranged by size:
1432 519 376 67
BsrBI CCG'CTC
Cuts at: 0 2016 2219 2394
Size: 2016 203 175
Fragments arranged by size:
2016 203 175
BsrFI r'CCGG_y
Cuts at: 0 1484 2394
Size: 1484 910
BstBI TT'CG_AA
Cuts at: 0 2367 2394
Size: 2367 27
BstEII G'GTnAC_C
Cuts at: 0 1755 2394
Size: 1755 639
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1123 1767 2394
Size: 1123 644 627
Fragments arranged by size:
1123 644 627
BstYI r'GATC_y
Cuts at: 0 652 801 2394
Size: 652 149 1593
Fragments arranged by size:
1593 652 149
Bsu36I CC'TnA_GG
Cuts at: 0 1546 2021 2394
Size: 1546 475 373
Fragments arranged by size:
1546 475 373
BtgI C'CryG_G
Cuts at: 0 1605 1826 2394
Size: 1605 221 568
Fragments arranged by size:
1605 568 221
BtsI GCAGTG_nn'
Cuts at: 0 647 2394
Size: 647 1747
DrdI GACnn_nn'nnGTC
Cuts at: 0 1047 2394
Size: 1047 1347
EaeI y'GGCC_r
Cuts at: 0 1530 1736 1904 1958 2394
Size: 1530 206 168 54 436
Fragments arranged by size:
1530 436 206 168 54
EagI C'GGCC_G
Cuts at: 0 1736 2394
Size: 1736 658
EarI CTCTTCn'nnn_
Cuts at: 0 2104 2110 2394
Size: 2104 6 284
Fragments arranged by size:
2104 284 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1145 2320 2394
Size: 1145 1175 74
Fragments arranged by size:
1175 1145 74
EcoO109I rG'GnC_Cy
Cuts at: 0 150 1566 1610 2188 2228 2394
Size: 150 1416 44 578 40 166
Fragments arranged by size:
1416 578 166 150 44 40
EcoRI G'AATT_C
Cuts at: 0 1064 2394
Size: 1064 1330
FspI TGC'GCA
Cuts at: 0 1873 2394
Size: 1873 521
FspAI rTGC'GCAy
Cuts at: 0 1873 2394
Size: 1873 521
HaeII r_GCGC'y
Cuts at: 0 1424 1598 1636 2394
Size: 1424 174 38 758
Fragments arranged by size:
1424 758 174 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 617 637 649 669 1105 1137
Size: 160 32 425 20 12 20 436 32
Cuts at: 1137 1420 1452 1594 1626 1768 1800 1981 2013
Size: 283 32 142 32 142 32 181 32
Cuts at: 2013 2394
Size: 381
Fragments arranged by size:
436 425 381 283 181 160 142 142
32 32 32 32 32 32 20 20
12
HincII GTy'rAC
Cuts at: 0 1458 2394
Size: 1458 936
KasI G'GCGC_C
Cuts at: 0 1594 2394
Size: 1594 800
KpnI G_GTAC'C
Cuts at: 0 437 2394
Size: 437 1957
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 465 677 2394
Size: 423 42 212 1717
Fragments arranged by size:
1717 423 212 42
MscI TGG'CCA
Cuts at: 0 1532 2394
Size: 1532 862
MslI CAynn'nnrTG
Cuts at: 0 640 691 1312 2394
Size: 640 51 621 1082
Fragments arranged by size:
1082 640 621 51
MspA1I CmG'CkG
Cuts at: 0 1607 1692 1815 2394
Size: 1607 85 123 579
Fragments arranged by size:
1607 579 123 85
NarI GG'CG_CC
Cuts at: 0 1595 2394
Size: 1595 799
NsiI A_TGCA'T
Cuts at: 0 852 2394
Size: 852 1542
PflMI CCAn_nnn'nTGG
Cuts at: 0 892 2394
Size: 892 1502
PfoI T'CCnGG_A
Cuts at: 0 775 2275 2394
Size: 775 1500 119
Fragments arranged by size:
1500 775 119
PmlI CAC'GTG
Cuts at: 0 184 1977 2394
Size: 184 1793 417
Fragments arranged by size:
1793 417 184
PpuMI rG'GwC_Cy
Cuts at: 0 150 1610 2188 2228 2394
Size: 150 1460 578 40 166
Fragments arranged by size:
1460 578 166 150 40
PstI C_TGCA'G
Cuts at: 0 1697 2394
Size: 1697 697
PvuII CAG'CTG
Cuts at: 0 1815 2394
Size: 1815 579
SacII CC_GC'GG
Cuts at: 0 1608 2394
Size: 1608 786
SanDI GG'GwC_CC
Cuts at: 0 1610 2188 2228 2394
Size: 1610 578 40 166
Fragments arranged by size:
1610 578 166 40
SapI GCTCTTCn'nnn_
Cuts at: 0 2104 2394
Size: 2104 290
SexAI A'CCwGG_T
Cuts at: 0 1751 2066 2394
Size: 1751 315 328
Fragments arranged by size:
1751 328 315
SfcI C'TryA_G
Cuts at: 0 1570 1693 2394
Size: 1570 123 701
Fragments arranged by size:
1570 701 123
SfoI GGC'GCC
Cuts at: 0 1596 2394
Size: 1596 798
SmaI CCC'GGG
Cuts at: 0 193 1732 1836 2394
Size: 193 1539 104 558
Fragments arranged by size:
1539 558 193 104
SmlI C'TyrA_G
Cuts at: 0 470 1819 2394
Size: 470 1349 575
Fragments arranged by size:
1349 575 470
SrfI GCCC'GGGC
Cuts at: 0 1836 2394
Size: 1836 558
SspI AAT'ATT
Cuts at: 0 47 954 2394
Size: 47 907 1440
Fragments arranged by size:
1440 907 47
StyI C'CwwG_G
Cuts at: 0 153 768 1505 1760 2394
Size: 153 615 737 255 634
Fragments arranged by size:
737 634 615 255 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 287 1299 1369 2394
Size: 287 1012 70 1025
Fragments arranged by size:
1025 1012 287 70
TatI w'GTAC_w
Cuts at: 0 1247 1301 2394
Size: 1247 54 1093
Fragments arranged by size:
1247 1093 54
Tth111I GACn'n_nGTC
Cuts at: 0 2232 2394
Size: 2232 162
XbaI T'CTAG_A
Cuts at: 0 399 2394
Size: 399 1995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1914 2394
Size: 1914 480
XmaI C'CCGG_G
Cuts at: 0 191 1730 1834 2394
Size: 191 1539 104 560
Fragments arranged by size:
1539 560 191 104
XmnI GAAnn'nnTTC
Cuts at: 0 860 1198 2394
Size: 860 338 1196
Fragments arranged by size:
1196 860 338
ZraI GAC'GTC
Cuts at: 0 1329 2394
Size: 1329 1065
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AhdI AleI AlwNI
ApoI AseI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EagI
EarI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII KasI KpnI MmeI MscI MslI MspA1I NarI
NsiI PflMI PfoI PmlI PpuMI PstI PvuII SacII
SanDI SapI SexAI SfcI SfoI SmaI SmlI SrfI
SspI StyI TaqII TatI Tth111I XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AlfI AloI ApaI
ApaLI AscI AsiSI BbvCI BglI BglII BmtI BsaBI
BsaWI BsiWI BspEI BsrDI BsrGI BssHII BssSI BstAPI
BstZ17I BtgZI ClaI CspCI DraI DraIII EciI EcoICRI
EcoNI EcoRV FalI FseI HindIII HpaI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI
PacI PasI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SbfI ScaI
SfiI SgfI SgrAI SnaBI SpeI SphI StuI SwaI
TstI XhoI