Restriction map for
pF1KB4219
(Linear) (Six-base) MAPSORT of: KIBB4219.seq Check: 4408 from: 1 to: 2823
KIBB4219.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 1229 2823
Size: 1229 1594
AccI GT'mk_AC
Cuts at: 0 242 1744 2823
Size: 242 1502 1079
Fragments arranged by size:
1502 1079 242
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 943 2823
Size: 943 1880
AflIII A'CryG_T
Cuts at: 0 1401 1841 2823
Size: 1401 440 982
Fragments arranged by size:
1401 982 440
AhdI GACnn_n'nnGTC
Cuts at: 0 909 2823
Size: 909 1914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 798 1302 2207 2823
Size: 798 504 905 616
Fragments arranged by size:
905 798 616 504
AlwNI CAG_nnn'CTG
Cuts at: 0 579 1425 2628 2823
Size: 579 846 1203 195
Fragments arranged by size:
1203 846 579 195
ApaI G_GGCC'C
Cuts at: 0 596 2790 2823
Size: 596 2194 33
Fragments arranged by size:
2194 596 33
ApaLI G'TGCA_C
Cuts at: 0 490 2823
Size: 490 2333
ApoI r'AATT_y
Cuts at: 0 1878 2485 2823
Size: 1878 607 338
Fragments arranged by size:
1878 607 338
AvaI C'yCGr_G
Cuts at: 0 227 608 770 850 1831 2041 2823
Size: 227 381 162 80 981 210 782
Fragments arranged by size:
981 782 381 227 210 162 80
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 416 449 2823
Size: 416 33 2374
Fragments arranged by size:
2374 416 33
BamHI G'GATC_C
Cuts at: 0 1766 1953 2823
Size: 1766 187 870
Fragments arranged by size:
1766 870 187
BanI G'GyrC_C
Cuts at: 0 2183 2763 2823
Size: 2183 580 60
Fragments arranged by size:
2183 580 60
BanII G_rGCy'C
Cuts at: 0 166 596 2030 2170 2790 2823
Size: 166 430 1434 140 620 33
Fragments arranged by size:
1434 620 430 166 140 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 650 908 2455 2823
Size: 78 572 258 1547 368
Fragments arranged by size:
1547 572 368 258 78
BbvCI CC'TCA_GC
Cuts at: 0 1270 2351 2823
Size: 1270 1081 472
Fragments arranged by size:
1270 1081 472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 703 737 816 850 1623 1657 2041 2075
Size: 703 34 79 34 773 34 384 34
Cuts at: 2075 2121 2155 2823
Size: 46 34 668
Fragments arranged by size:
773 703 668 384 79 46 34 34
34 34 34
BclI T'GATC_A
Cuts at: 0 2238 2823
Size: 2238 585
BglII A'GATC_T
Cuts at: 0 1283 2054 2823
Size: 1283 771 769
Fragments arranged by size:
1283 771 769
BlpI GC'TnA_GC
Cuts at: 0 65 2823
Size: 65 2758
Bme1580I G_kGCm'C
Cuts at: 0 494 596 2790 2823
Size: 494 102 2194 33
Fragments arranged by size:
2194 494 102 33
BmgBI CAC'GTC
Cuts at: 0 1844 2823
Size: 1844 979
BmtI G_CTAG'C
Cuts at: 0 451 2823
Size: 451 2372
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1305 2823
Size: 1305 1518
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 1225 2044 2323 2527 2823
Size: 799 426 819 279 204 296
Fragments arranged by size:
819 799 426 296 279 204
Bpu10I CC'TnA_GC
Cuts at: 0 1270 1858 2195 2351 2567 2823
Size: 1270 588 337 156 216 256
Fragments arranged by size:
1270 588 337 256 216 156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1150 2823
Size: 76 1074 1673
Fragments arranged by size:
1673 1074 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 760 1734 2495 2823
Size: 760 974 761 328
Fragments arranged by size:
974 761 760 328
BsaAI yAC'GTr
Cuts at: 0 1404 2823
Size: 1404 1419
BsaBI GATnn'nnATC
Cuts at: 0 1372 2605 2823
Size: 1372 1233 218
Fragments arranged by size:
1372 1233 218
BsaHI Gr'CG_yC
Cuts at: 0 146 1226 2823
Size: 146 1080 1597
Fragments arranged by size:
1597 1080 146
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 196 226 830 860 919 949 2823
Size: 196 30 604 30 59 30 1874
Fragments arranged by size:
1874 604 196 59 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2386 2395 2767 2823
Size: 2386 9 372 56
Fragments arranged by size:
2386 372 56 9
BseYI C'CCAG_C
Cuts at: 0 269 348 363 463 2823
Size: 269 79 15 100 2360
Fragments arranged by size:
2360 269 100 79 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 2823
Size: 592 2231
BsiEI CG_ry'CG
Cuts at: 0 61 675 681 1382 2367 2823
Size: 61 614 6 701 985 456
Fragments arranged by size:
985 701 614 456 61 6
BsiHKAI G_wGCw'C
Cuts at: 0 224 494 749 851 1130 2030 2594 2681
Size: 224 270 255 102 279 900 564 87
Cuts at: 2681 2823
Size: 142
Fragments arranged by size:
900 564 279 270 255 224 142 102
87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1233 2823
Size: 1233 1590
Bsp1286I G_dGCh'C
Cuts at: 0 166 224 494 596 749 851 1130 2030
Size: 166 58 270 102 153 102 279 900
Cuts at: 2030 2170 2594 2681 2790 2823
Size: 140 424 87 109 33
Fragments arranged by size:
900 424 279 270 166 153 140 109
102 102 87 58 33
BspHI T'CATG_A
Cuts at: 0 2085 2408 2823
Size: 2085 323 415
Fragments arranged by size:
2085 415 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 72 386 564 764 2015 2823
Size: 72 314 178 200 1251 808
Fragments arranged by size:
1251 808 314 200 178 72
BsrDI GCAATG_nn'
Cuts at: 0 363 2594 2823
Size: 363 2231 229
Fragments arranged by size:
2231 363 229
BsrFI r'CCGG_y
Cuts at: 0 60 1483 2823
Size: 60 1423 1340
Fragments arranged by size:
1423 1340 60
BsrGI T'GTAC_A
Cuts at: 0 945 2823
Size: 945 1878
BstAPI GCAn_nnn'nTGC
Cuts at: 0 579 987 2823
Size: 579 408 1836
Fragments arranged by size:
1836 579 408
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1140 2307 2823
Size: 405 735 1167 516
Fragments arranged by size:
1167 735 516 405
BstYI r'GATC_y
Cuts at: 0 686 1283 1367 1421 1766 1953 2054 2274
Size: 686 597 84 54 345 187 101 220
Cuts at: 2274 2823
Size: 549
Fragments arranged by size:
686 597 549 345 220 187 101 84
54
BstZ17I GTA'TAC
Cuts at: 0 1745 2823
Size: 1745 1078
BtgI C'CryG_G
Cuts at: 0 70 470 1412 1464 1579 1847 2220 2516
Size: 70 400 942 52 115 268 373 296
Cuts at: 2516 2712 2823
Size: 196 111
Fragments arranged by size:
942 400 373 296 268 196 115 111
70 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1377 1626 2187 2614 2823
Size: 1377 249 561 427 209
Fragments arranged by size:
1377 561 427 249 209
BtsI GCAGTG_nn'
Cuts at: 0 1101 2823
Size: 1101 1722
DrdI GACnn_nn'nnGTC
Cuts at: 0 1231 2691 2823
Size: 1231 1460 132
Fragments arranged by size:
1460 1231 132
EaeI y'GGCC_r
Cuts at: 0 15 395 498 1322 1379 1550 1697 1850
Size: 15 380 103 824 57 171 147 153
Cuts at: 1850 2297 2823
Size: 447 526
Fragments arranged by size:
824 526 447 380 171 153 147 103
57 15
EagI C'GGCC_G
Cuts at: 0 1379 2823
Size: 1379 1444
EarI CTCTTCn'nnn_
Cuts at: 0 732 851 1190 1213 2823
Size: 732 119 339 23 1610
Fragments arranged by size:
1610 732 339 119 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 655 2823
Size: 655 2168
EcoICRI GAG'CTC
Cuts at: 0 2028 2823
Size: 2028 795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 799 943 1225 2044 2323 2527 2823
Size: 799 144 282 819 279 204 296
Fragments arranged by size:
819 799 296 282 279 204 144
EcoNI CCTnn'n_nnAGG
Cuts at: 0 396 760 889 1240 1831 2509 2749 2823
Size: 396 364 129 351 591 678 240 74
Fragments arranged by size:
678 591 396 364 351 240 129 74
EcoO109I rG'GnC_Cy
Cuts at: 0 207 285 576 927 1602 2334 2625 2786
Size: 207 78 291 351 675 732 291 161
Cuts at: 2786 2787 2823
Size: 1 36
Fragments arranged by size:
732 675 351 291 291 207 161 78
36 1
EcoRI G'AATT_C
Cuts at: 0 2485 2823
Size: 2485 338
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1224 2823
Size: 1224 1599
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 250 282 895 919 927 951
Size: 196 32 22 32 613 24 8 24
Cuts at: 951 1213 1245 1828 1860 2230 2262 2381 2413
Size: 262 32 583 32 370 32 119 32
Cuts at: 2413 2823
Size: 410
Fragments arranged by size:
613 583 410 370 262 196 119 32
32 32 32 32 32 24 24 22
8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 717 782 1757 2693 2823
Size: 717 65 975 936 130
Fragments arranged by size:
975 936 717 130 65
MscI TGG'CCA
Cuts at: 0 397 500 1324 1699 1852 2299 2823
Size: 397 103 824 375 153 447 524
Fragments arranged by size:
824 524 447 397 375 153 103
MslI CAynn'nnrTG
Cuts at: 0 487 1220 1546 1846 2599 2823
Size: 487 733 326 300 753 224
Fragments arranged by size:
753 733 487 326 300 224
MspA1I CmG'CkG
Cuts at: 0 72 217 273 478 504 782 921 954
Size: 72 145 56 205 26 278 139 33
Cuts at: 954 1466 2714 2823
Size: 512 1248 109
Fragments arranged by size:
1248 512 278 205 145 139 109 72
56 33 26
NcoI C'CATG_G
Cuts at: 0 1579 1847 2516 2823
Size: 1579 268 669 307
Fragments arranged by size:
1579 669 307 268
NdeI CA'TA_TG
Cuts at: 0 1443 2823
Size: 1443 1380
NheI G'CTAG_C
Cuts at: 0 447 2823
Size: 447 2376
NspI r_CATG'y
Cuts at: 0 14 966 1093 1866 2082 2271 2823
Size: 14 952 127 773 216 189 552
Fragments arranged by size:
952 773 552 216 189 127 14
PasI CC'CwG_GG
Cuts at: 0 596 2496 2823
Size: 596 1900 327
Fragments arranged by size:
1900 596 327
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 897 1029 1776 2346 2444 2823
Size: 435 462 132 747 570 98 379
Fragments arranged by size:
747 570 462 435 379 132 98
PfoI T'CCnGG_A
Cuts at: 0 320 1241 2248 2744 2823
Size: 320 921 1007 496 79
Fragments arranged by size:
1007 921 496 320 79
PmlI CAC'GTG
Cuts at: 0 1404 2823
Size: 1404 1419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1148 1180 2823
Size: 1148 32 1643
Fragments arranged by size:
1643 1148 32
PpuMI rG'GwC_Cy
Cuts at: 0 285 576 927 1602 2334 2625 2823
Size: 285 291 351 675 732 291 198
Fragments arranged by size:
732 675 351 291 291 285 198
PspOMI G'GGCC_C
Cuts at: 0 592 2786 2823
Size: 592 2194 37
Fragments arranged by size:
2194 592 37
PspXI vC'TCGA_Gb
Cuts at: 0 2041 2823
Size: 2041 782
PstI C_TGCA'G
Cuts at: 0 34 143 533 584 737 761 959 983
Size: 34 109 390 51 153 24 198 24
Cuts at: 983 1871 2809 2823
Size: 888 938 14
Fragments arranged by size:
938 888 390 198 153 109 51 34
24 24 14
PvuII CAG'CTG
Cuts at: 0 217 273 478 504 921 954 2823
Size: 217 56 205 26 417 33 1869
Fragments arranged by size:
1869 417 217 205 56 33 26
SacI G_AGCT'C
Cuts at: 0 2030 2823
Size: 2030 793
SacII CC_GC'GG
Cuts at: 0 73 1467 2715 2823
Size: 73 1394 1248 108
Fragments arranged by size:
1394 1248 108 73
SapI GCTCTTCn'nnn_
Cuts at: 0 1190 2823
Size: 1190 1633
SbfI CC_TGCA'GG
Cuts at: 0 143 533 2823
Size: 143 390 2290
Fragments arranged by size:
2290 390 143
SexAI A'CCwGG_T
Cuts at: 0 1022 2823
Size: 1022 1801
SfcI C'TryA_G
Cuts at: 0 30 139 529 580 733 757 955 979
Size: 30 109 390 51 153 24 198 24
Cuts at: 979 1867 2154 2805 2823
Size: 888 287 651 18
Fragments arranged by size:
888 651 390 287 198 153 109 51
30 24 24 18
SmaI CCC'GGG
Cuts at: 0 772 2823
Size: 772 2051
SmlI C'TyrA_G
Cuts at: 0 91 1165 2041 2823
Size: 91 1074 876 782
Fragments arranged by size:
1074 876 782 91
SphI G_CATG'C
Cuts at: 0 14 1866 2271 2823
Size: 14 1852 405 552
Fragments arranged by size:
1852 552 405 14
StuI AGG'CCT
Cuts at: 0 185 2557 2823
Size: 185 2372 266
Fragments arranged by size:
2372 266 185
StyI C'CwwG_G
Cuts at: 0 392 1034 1325 1579 1847 2438 2516 2687
Size: 392 642 291 254 268 591 78 171
Cuts at: 2687 2823
Size: 136
Fragments arranged by size:
642 591 392 291 268 254 171 136
78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1228 2233 2381 2823
Size: 1228 1005 148 442
Fragments arranged by size:
1228 1005 442 148
TatI w'GTAC_w
Cuts at: 0 945 2823
Size: 945 1878
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 830 862 2823
Size: 830 32 1961
Fragments arranged by size:
1961 830 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1023 2823
Size: 1023 1800
XhoI C'TCGA_G
Cuts at: 0 2041 2823
Size: 2041 782
XmaI C'CCGG_G
Cuts at: 0 770 2823
Size: 770 2053
XmnI GAAnn'nnTTC
Cuts at: 0 1207 2823
Size: 1207 1616
ZraI GAC'GTC
Cuts at: 0 1227 2823
Size: 1227 1596
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BclI BglII BlpI Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
MmeI MscI MslI MspA1I NcoI NdeI NheI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI
PstI PvuII SacI SacII SapI SbfI SexAI SfcI
SmaI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AloI
AscI AseI AsiSI AvrII BbeI BciVI BfrBI BglI
BmrI BsaWI BsiWI BsmI BspEI BsrBI BssHII BssSI
BstBI BstEII Bsu36I ClaI CspCI DraI DraIII EcoRV
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NgoMIV NotI NruI
NsiI PacI PciI PmeI PshAI PsiI PsrI PvuI
RsrII SalI SanDI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI