Restriction map for
pF1KB4220
(Linear) (Six-base) MAPSORT of: KIBB4220.seq Check: 3138 from: 1 to: 1041
KIBB4220.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 0 618 1041
Size: 618 423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 245 352 1041
Size: 235 10 107 689
Fragments arranged by size:
689 235 107 10
AflII C'TTAA_G
Cuts at: 0 109 1041
Size: 109 932
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 1041
Size: 935 106
AlwNI CAG_nnn'CTG
Cuts at: 0 573 1041
Size: 573 468
ApaI G_GGCC'C
Cuts at: 0 903 1041
Size: 903 138
AvaI C'yCGr_G
Cuts at: 0 857 1041
Size: 857 184
BamHI G'GATC_C
Cuts at: 0 892 1041
Size: 892 149
BanI G'GyrC_C
Cuts at: 0 665 1041
Size: 665 376
BanII G_rGCy'C
Cuts at: 0 838 903 1041
Size: 838 65 138
Fragments arranged by size:
838 138 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 834 1041
Size: 834 207
Bme1580I G_kGCm'C
Cuts at: 0 903 1041
Size: 903 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 636 738 1041
Size: 636 102 303
Fragments arranged by size:
636 303 102
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 852 1041
Size: 852 189
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1041
Size: 928 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 286 1041
Size: 286 755
BsaBI GATnn'nnATC
Cuts at: 0 992 1041
Size: 992 49
BsaWI w'CCGG_w
Cuts at: 0 428 1041
Size: 428 613
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 543 573 1041
Size: 543 30 468
Fragments arranged by size:
543 468 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1024 1027 1036 1041
Size: 1024 3 9 5
Fragments arranged by size:
1024 9 5 3
BseYI C'CCAG_C
Cuts at: 0 642 902 1041
Size: 642 260 139
Fragments arranged by size:
642 260 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1041
Size: 627 414
BsiHKAI G_wGCw'C
Cuts at: 0 522 838 914 1041
Size: 522 316 76 127
Fragments arranged by size:
522 316 127 76
BsmI GAATG_Cn'
Cuts at: 0 586 1041
Size: 586 455
Bsp1286I G_dGCh'C
Cuts at: 0 522 838 903 914 1041
Size: 522 316 65 11 127
Fragments arranged by size:
522 316 127 65 11
BspEI T'CCGG_A
Cuts at: 0 428 1041
Size: 428 613
BsrDI GCAATG_nn'
Cuts at: 0 1037 1041
Size: 1037 4
BstEII G'GTnAC_C
Cuts at: 0 778 1041
Size: 778 263
BstXI CCAn_nnnn'nTGG
Cuts at: 0 82 403 896 1041
Size: 82 321 493 145
Fragments arranged by size:
493 321 145 82
BstYI r'GATC_y
Cuts at: 0 892 1041
Size: 892 149
Bsu36I CC'TnA_GG
Cuts at: 0 209 1041
Size: 209 832
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 9 44 1041
Size: 9 35 997
Fragments arranged by size:
997 35 9
DraIII CAC_nnn'GTG
Cuts at: 0 193 1041
Size: 193 848
DrdI GACnn_nn'nnGTC
Cuts at: 0 419 1041
Size: 419 622
EaeI y'GGCC_r
Cuts at: 0 702 1041
Size: 702 339
EarI CTCTTCn'nnn_
Cuts at: 0 1013 1041
Size: 1013 28
EcoICRI GAG'CTC
Cuts at: 0 836 1041
Size: 836 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 245 352 928 1041
Size: 235 10 107 576 113
Fragments arranged by size:
576 235 113 107 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 407 877 1041
Size: 407 470 164
Fragments arranged by size:
470 407 164
EcoO109I rG'GnC_Cy
Cuts at: 0 87 213 229 478 1041
Size: 87 126 16 249 563
Fragments arranged by size:
563 249 126 87 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 840 872 877 909 958 990 1041
Size: 840 32 5 32 49 32 51
Fragments arranged by size:
840 51 49 32 32 32 5
HindIII A'AGCT_T
Cuts at: 0 202 1041
Size: 202 839
MscI TGG'CCA
Cuts at: 0 704 1041
Size: 704 337
MslI CAynn'nnrTG
Cuts at: 0 823 917 1041
Size: 823 94 124
Fragments arranged by size:
823 124 94
MspA1I CmG'CkG
Cuts at: 0 443 906 1041
Size: 443 463 135
Fragments arranged by size:
463 443 135
PasI CC'CwG_GG
Cuts at: 0 82 1041
Size: 82 959
PflMI CCAn_nnn'nTGG
Cuts at: 0 363 573 1041
Size: 363 210 468
Fragments arranged by size:
468 363 210
PpuMI rG'GwC_Cy
Cuts at: 0 87 1041
Size: 87 954
PspOMI G'GGCC_C
Cuts at: 0 899 1041
Size: 899 142
PvuII CAG'CTG
Cuts at: 0 443 906 1041
Size: 443 463 135
Fragments arranged by size:
463 443 135
SacI G_AGCT'C
Cuts at: 0 838 1041
Size: 838 203
SanDI GG'GwC_CC
Cuts at: 0 87 1041
Size: 87 954
SfcI C'TryA_G
Cuts at: 0 140 1041
Size: 140 901
SmlI C'TyrA_G
Cuts at: 0 109 1041
Size: 109 932
StyI C'CwwG_G
Cuts at: 0 474 1041
Size: 474 567
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 527 720 1041
Size: 527 193 321
Fragments arranged by size:
527 321 193
TatI w'GTAC_w
Cuts at: 0 175 1041
Size: 175 866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 512 543 544 575 1041
Size: 512 31 1 31 466
Fragments arranged by size:
512 466 31 31 1
Enzymes that do cut:
AccI AcuI AflII AlfI AlwNI ApaI AvaI BamHI
BanI BanII BbsI Bme1580I BmrI BplI BpmI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BsrDI BstEII BstXI BstYI Bsu36I CspCI
DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HindIII MscI MslI MspA1I PasI PflMI PpuMI
PspOMI PvuII SacI SanDI SfcI SmlI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaAI BsaHI
BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstZ17I BtgI BtgZI BtsI
ClaI DraI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI