Restriction map for pF1KB4221
 (Linear) (Six-base) MAPSORT of: KIBB4221.seq  Check: 862  from: 1  to: 1683



KIBB4221.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AccI GT'mk_AC

Cuts at:      0     173    1683 
   Size:        173    1510
 
Acc65I G'GTAC_C

Cuts at:      0     112     807    1683 
   Size:        112     695     876

  Fragments arranged by size: 

                 876     695     112
 
AclI AA'CG_TT

Cuts at:      0     275    1683 
   Size:        275    1408
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29    1099    1109    1183    1683 
   Size:         29    1070      10      74     500

  Fragments arranged by size: 

                1070     500      74      29      10
 
AleI CACnn'nnGTG

Cuts at:      0    1621    1683 
   Size:       1621      62
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1393    1425    1540    1572    1683 
   Size:       1393      32     115      32     111

  Fragments arranged by size: 

                1393     115     111      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57     129     989    1177    1644    1683 
   Size:         57      72     860     188     467      39

  Fragments arranged by size: 

                 860     467     188      72      57      39
 
ApaI G_GGCC'C

Cuts at:      0     669     691    1683 
   Size:        669      22     992

  Fragments arranged by size: 

                 992     669      22
 
AvaI C'yCGr_G

Cuts at:      0     662     780    1022    1427    1683 
   Size:        662     118     242     405     256

  Fragments arranged by size: 

                 662     405     256     242     118
 
AvrII C'CTAG_G

Cuts at:      0    1678    1683 
   Size:       1678       5
 
BanI G'GyrC_C

Cuts at:      0      46     112     211     807    1350    1655    1683 
   Size:         46      66      99     596     543     305      28

  Fragments arranged by size: 

                 596     543     305      99      66      46      28
 
BanII G_rGCy'C

Cuts at:      0     572     669     691     920    1046    1439    1683 
   Size:        572      97      22     229     126     393     244

  Fragments arranged by size: 

                 572     393     244     229     126      97      22
 
BbeI G_GCGC'C

Cuts at:      0     215    1683 
   Size:        215    1468
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     521    1362    1683 
   Size:        521     841     321

  Fragments arranged by size: 

                 841     521     321
 
BbvCI CC'TCA_GC

Cuts at:      0     254     752    1683 
   Size:        254     498     931

  Fragments arranged by size: 

                 931     498     254
 
BfrBI ATG'CAT

Cuts at:      0      18    1683 
   Size:         18    1665
 
BglI GCCn_nnn'nGGC

Cuts at:      0     576     967    1683 
   Size:        576     391     716

  Fragments arranged by size: 

                 716     576     391
 
BlpI GC'TnA_GC

Cuts at:      0     791    1683 
   Size:        791     892
 
Bme1580I G_kGCm'C

Cuts at:      0      51     629     669     691     781    1136    1683 
   Size:         51     578      40      22      90     355     547

  Fragments arranged by size: 

                 578     547     355      90      51      40      22
 
BmgBI CAC'GTC

Cuts at:      0     266    1683 
   Size:        266    1417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     616    1682    1683 
   Size:        616    1066       1

  Fragments arranged by size: 

                1066     616       1
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     558     576    1566    1683 
   Size:        558      18     990     117

  Fragments arranged by size: 

                 990     558     117      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     417     571     966    1222    1585    1683 
   Size:        417     154     395     256     363      98

  Fragments arranged by size: 

                 417     395     363     256     154      98
 
Bpu10I CC'TnA_GC

Cuts at:      0     254     719     752    1683 
   Size:        254     465      33     931

  Fragments arranged by size: 

                 931     465     254      33
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     985    1683 
   Size:        601     384     698

  Fragments arranged by size: 

                 698     601     384
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     253    1683 
   Size:        253    1430
 
BsaAI yAC'GTr

Cuts at:      0     320    1623    1683 
   Size:        320    1303      60

  Fragments arranged by size: 

                1303     320      60
 
BsaHI Gr'CG_yC

Cuts at:      0     212    1683 
   Size:        212    1471
 
BsaWI w'CCGG_w

Cuts at:      0     872    1460    1683 
   Size:        872     588     223

  Fragments arranged by size: 

                 872     588     223
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     250     280     854     884    1683 
   Size:        250      30     574      30     799

  Fragments arranged by size: 

                 799     574     250      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     271     327     358     361    1458    1683 
   Size:        271      56      31       3    1097     225

  Fragments arranged by size: 

                1097     271     225      56      31       3
 
BseYI C'CCAG_C

Cuts at:      0     116     907    1630    1683 
   Size:        116     791     723      53

  Fragments arranged by size: 

                 791     723     116      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1683 
   Size:         82    1601
 
BsiHKAI G_wGCw'C

Cuts at:      0     527     850    1610    1683 
   Size:        527     323     760      73

  Fragments arranged by size: 

                 760     527     323      73
 
Bsp1286I G_dGCh'C

Cuts at:      0      51     527     572     629     669     691     781     850 
   Size:         51     476      45      57      40      22      90      69

Cuts at:    850     920    1046    1136    1439    1610    1683 
   Size:         70     126      90     303     171      73

  Fragments arranged by size: 

                 476     303     171     126      90      90      73      70
                  69      57      51      45      40      22
 
BspEI T'CCGG_A

Cuts at:      0     872    1460    1683 
   Size:        872     588     223

  Fragments arranged by size: 

                 872     588     223
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65     104     419     819    1683 
   Size:         65      39     315     400     864

  Fragments arranged by size: 

                 864     400     315      65      39
 
BsrBI CCG'CTC

Cuts at:      0     471    1683 
   Size:        471    1212
 
BsrFI r'CCGG_y

Cuts at:      0     603    1683 
   Size:        603    1080
 
Bsu36I CC'TnA_GG

Cuts at:      0    1178    1683 
   Size:       1178     505
 
BtgI C'CryG_G

Cuts at:      0     503     933    1139    1184    1382    1683 
   Size:        503     430     206      45     198     301

  Fragments arranged by size: 

                 503     430     301     206     198      45
 
BtsI GCAGTG_nn'

Cuts at:      0     971    1354    1683 
   Size:        971     383     329

  Fragments arranged by size: 

                 971     383     329
 
EaeI y'GGCC_r

Cuts at:      0      80     387     930     968    1683 
   Size:         80     307     543      38     715

  Fragments arranged by size: 

                 715     543     307      80      38
 
EarI CTCTTCn'nnn_

Cuts at:      0       5    1085    1683 
   Size:          5    1080     598

  Fragments arranged by size: 

                1080     598       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     417     571     966    1099    1109    1183    1222 
   Size:         29     388     154     395     133      10      74      39

Cuts at:   1222    1585    1683 
   Size:        363      98

  Fragments arranged by size: 

                 395     388     363     154     133      98      74      39
                  29      10
 
EcoO109I rG'GnC_Cy

Cuts at:      0     687     688     768     938    1095    1488    1575    1683 
   Size:        687       1      80     170     157     393      87     108

  Fragments arranged by size: 

                 687     393     170     157     108      87      80       1
            
 
EcoRV GAT'ATC

Cuts at:      0     483    1683 
   Size:        483    1200
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     663    1683 
   Size:        663    1020
 
FspI TGC'GCA

Cuts at:      0     376    1683 
   Size:        376    1307
 
HaeII r_GCGC'y

Cuts at:      0     215    1683 
   Size:        215    1468
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     250     282     421     453     819     851    1495    1515 
   Size:        250      32     139      32     366      32     644      20

Cuts at:   1515    1527    1547    1683 
   Size:         12      20     136

  Fragments arranged by size: 

                 644     366     250     139     136      32      32      32
                  20      20      12
 
HincII GTy'rAC

Cuts at:      0    1377    1683 
   Size:       1377     306
 
KasI G'GCGC_C

Cuts at:      0     211    1683 
   Size:        211    1472
 
KpnI G_GTAC'C

Cuts at:      0     116     811    1683 
   Size:        116     695     872

  Fragments arranged by size: 

                 872     695     116
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293    1683 
   Size:        293    1390
 
MscI TGG'CCA

Cuts at:      0      82     389     932     970    1683 
   Size:         82     307     543      38     713

  Fragments arranged by size: 

                 713     543     307      82      38
 
MslI CAynn'nnrTG

Cuts at:      0     530    1621    1683 
   Size:        530    1091      62

  Fragments arranged by size: 

                1091     530      62
 
MspA1I CmG'CkG

Cuts at:      0     120     126    1683 
   Size:        120       6    1557

  Fragments arranged by size: 

                1557     120       6
 
NarI GG'CG_CC

Cuts at:      0     212    1683 
   Size:        212    1471
 
NcoI C'CATG_G

Cuts at:      0    1184    1683 
   Size:       1184     499
 
NsiI A_TGCA'T

Cuts at:      0      20    1683 
   Size:         20    1663
 
NspI r_CATG'y

Cuts at:      0     660    1683 
   Size:        660    1023
 
PasI CC'CwG_GG

Cuts at:      0     803    1683 
   Size:        803     880
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     739    1683 
   Size:        739     944
 
PfoI T'CCnGG_A

Cuts at:      0     890    1683 
   Size:        890     793
 
PmlI CAC'GTG

Cuts at:      0     320    1623    1683 
   Size:        320    1303      60

  Fragments arranged by size: 

                1303     320      60
 
PpuMI rG'GwC_Cy

Cuts at:      0     768    1095    1488    1575    1683 
   Size:        768     327     393      87     108

  Fragments arranged by size: 

                 768     393     327     108      87
 
PspOMI G'GGCC_C

Cuts at:      0     665     687    1683 
   Size:        665      22     996

  Fragments arranged by size: 

                 996     665      22
 
PstI C_TGCA'G

Cuts at:      0     125    1683 
   Size:        125    1558
 
PvuII CAG'CTG

Cuts at:      0     120     126    1683 
   Size:        120       6    1557

  Fragments arranged by size: 

                1557     120       6
 
SanDI GG'GwC_CC

Cuts at:      0     768    1683 
   Size:        768     915
 
SfcI C'TryA_G

Cuts at:      0     121    1683 
   Size:        121    1562
 
SfoI GGC'GCC

Cuts at:      0     213    1683 
   Size:        213    1470
 
SmaI CCC'GGG

Cuts at:      0     664    1429    1683 
   Size:        664     765     254

  Fragments arranged by size: 

                 765     664     254
 
SmlI C'TyrA_G

Cuts at:      0     580    1000    1683 
   Size:        580     420     683

  Fragments arranged by size: 

                 683     580     420
 
StyI C'CwwG_G

Cuts at:      0      83     416     683    1102    1184    1678    1683 
   Size:         83     333     267     419      82     494       5

  Fragments arranged by size: 

                 494     419     333     267      83      82       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     614    1047    1356    1633    1683 
   Size:        614     433     309     277      50

  Fragments arranged by size: 

                 614     433     309     277      50
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     760     792     854     886    1115    1147    1683 
   Size:        760      32      62      32     229      32     536

  Fragments arranged by size: 

                 760     536     229      62      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     264    1683 
   Size:        264    1419
 
XmaI C'CCGG_G

Cuts at:      0     662    1427    1683 
   Size:        662     765     256

  Fragments arranged by size: 

                 765     662     256
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AleI     AloI    AlwNI     ApaI
     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI   Bsu36I
     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NsiI     NspI     PasI    PflMI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII    SanDI     SfcI
     SfoI     SmaI     SmlI     StyI    TaqII     TstI  Tth111I     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII   AflIII     AgeI     AhdI     AlfI
    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI    BamHI     BcgI
    BciVI     BclI    BglII     BmtI    BsaBI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FseI
    FspAI  HindIII     HpaI     MfeI     MluI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI
     XcmI     XhoI     XmnI     ZraI
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