Restriction map for
pF1KB4231
(Linear) (Six-base) MAPSORT of: KIBB4231.seq Check: 8139 from: 1 to: 5547
KIBB4231.orf
With 182 enzymes: SgfI *
June 20, 2019 10:05 ..
AccI GT'mk_AC
Cuts at: 0 2889 3495 3632 5547
Size: 2889 606 137 1915
Fragments arranged by size:
2889 1915 606 137
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 811 908 1202 1576 2210 2618 2930 3163
Size: 811 97 294 374 634 408 312 233
Cuts at: 3163 3672 4139 5179 5547
Size: 509 467 1040 368
Fragments arranged by size:
1040 811 634 509 467 408 374 368
312 294 233 97
AflII C'TTAA_G
Cuts at: 0 3708 5547
Size: 3708 1839
AflIII A'CryG_T
Cuts at: 0 26 529 5547
Size: 26 503 5018
Fragments arranged by size:
5018 503 26
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2657 2689 3875 3907 5547
Size: 2657 32 1186 32 1640
Fragments arranged by size:
2657 1640 1186 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 844 1653 5547
Size: 844 809 3894
Fragments arranged by size:
3894 844 809
ApaI G_GGCC'C
Cuts at: 0 421 5547
Size: 421 5126
ApoI r'AATT_y
Cuts at: 0 216 494 1121 1212 1539 1586 1995 2269
Size: 216 278 627 91 327 47 409 274
Cuts at: 2269 2407 3123 3268 3557 3985 4540 4819 5547
Size: 138 716 145 289 428 555 279 728
Fragments arranged by size:
728 716 627 555 428 409 327 289
279 278 274 216 145 138 91 47
AseI AT'TA_AT
Cuts at: 0 376 1433 2398 5547
Size: 376 1057 965 3149
Fragments arranged by size:
3149 1057 965 376
AvaI C'yCGr_G
Cuts at: 0 38 5547
Size: 38 5509
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1974 2007 2897 2930 5547
Size: 1974 33 890 33 2617
Fragments arranged by size:
2617 1974 890 33 33
BanI G'GyrC_C
Cuts at: 0 2179 2799 5547
Size: 2179 620 2748
Fragments arranged by size:
2748 2179 620
BanII G_rGCy'C
Cuts at: 0 45 421 5547
Size: 45 376 5126
Fragments arranged by size:
5126 376 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 22 817 2359 2727 4127 4896 5547
Size: 22 795 1542 368 1400 769 651
Fragments arranged by size:
1542 1400 795 769 651 368 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 2457 4680 5547
Size: 2457 2223 867
Fragments arranged by size:
2457 2223 867
BclI T'GATC_A
Cuts at: 0 762 894 1626 3861 5547
Size: 762 132 732 2235 1686
Fragments arranged by size:
2235 1686 762 732 132
BfrBI ATG'CAT
Cuts at: 0 2293 2963 2992 5398 5547
Size: 2293 670 29 2406 149
Fragments arranged by size:
2406 2293 670 149 29
BlpI GC'TnA_GC
Cuts at: 0 4448 5547
Size: 4448 1099
Bme1580I G_kGCm'C
Cuts at: 0 332 421 3908 5547
Size: 332 89 3487 1639
Fragments arranged by size:
3487 1639 332 89
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 176 5547
Size: 176 5371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 332 686 2023 3001 3206 4658 5547
Size: 67 265 354 1337 978 205 1452 889
Fragments arranged by size:
1452 1337 978 889 354 265 205 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1754 2222 3660 3782 4979 5547
Size: 1754 468 1438 122 1197 568
Fragments arranged by size:
1754 1438 1197 568 468 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 3557 3749 5161 5547
Size: 3557 192 1412 386
Fragments arranged by size:
3557 1412 386 192
BsaHI Gr'CG_yC
Cuts at: 0 2860 5547
Size: 2860 2687
BsaWI w'CCGG_w
Cuts at: 0 3037 5204 5547
Size: 3037 2167 343
Fragments arranged by size:
3037 2167 343
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2141 2171 3284 3314 5547
Size: 2141 30 1113 30 2233
Fragments arranged by size:
2233 2141 1113 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 149 5257 5547
Size: 149 5108 290
Fragments arranged by size:
5108 290 149
BseYI C'CCAG_C
Cuts at: 0 92 2632 4911 5547
Size: 92 2540 2279 636
Fragments arranged by size:
2540 2279 636 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1079 1688 4486 4560 4888 5547
Size: 890 189 609 2798 74 328 659
Fragments arranged by size:
2798 890 659 609 328 189 74
BsiEI CG_ry'CG
Cuts at: 0 2394 5547
Size: 2394 3153
BsmI GAATG_Cn'
Cuts at: 0 1401 1846 2434 2875 2972 3889 5195 5547
Size: 1401 445 588 441 97 917 1306 352
Fragments arranged by size:
1401 1306 917 588 445 441 352 97
Bsp1286I G_dGCh'C
Cuts at: 0 45 332 421 3908 5547
Size: 45 287 89 3487 1639
Fragments arranged by size:
3487 1639 287 89 45
BspEI T'CCGG_A
Cuts at: 0 5204 5547
Size: 5204 343
BspHI T'CATG_A
Cuts at: 0 498 1341 4716 5547
Size: 498 843 3375 831
Fragments arranged by size:
3375 843 831 498
BsrDI GCAATG_nn'
Cuts at: 0 348 1366 4002 5547
Size: 348 1018 2636 1545
Fragments arranged by size:
2636 1545 1018 348
BsrGI T'GTAC_A
Cuts at: 0 984 5210 5547
Size: 984 4226 337
Fragments arranged by size:
4226 984 337
BssSI C'ACGA_G
Cuts at: 0 715 5547
Size: 715 4832
BstAPI GCAn_nnn'nTGC
Cuts at: 0 107 4378 5547
Size: 107 4271 1169
Fragments arranged by size:
4271 1169 107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5178 5547
Size: 5178 369
BstYI r'GATC_y
Cuts at: 0 853 1329 3232 3391 5547
Size: 853 476 1903 159 2156
Fragments arranged by size:
2156 1903 853 476 159
Bsu36I CC'TnA_GG
Cuts at: 0 1822 4073 5547
Size: 1822 2251 1474
Fragments arranged by size:
2251 1822 1474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4112 5547
Size: 4112 1435
BtsI GCAGTG_nn'
Cuts at: 0 3974 5280 5547
Size: 3974 1306 267
Fragments arranged by size:
3974 1306 267
ClaI AT'CG_AT
Cuts at: 0 2390 2394 5547
Size: 2390 4 3153
Fragments arranged by size:
3153 2390 4
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4721 4756 5547
Size: 4721 35 791
Fragments arranged by size:
4721 791 35
DraI TTT'AAA
Cuts at: 0 1581 1994 5388 5547
Size: 1581 413 3394 159
Fragments arranged by size:
3394 1581 413 159
DraIII CAC_nnn'GTG
Cuts at: 0 1234 5547
Size: 1234 4313
DrdI GACnn_nn'nnGTC
Cuts at: 0 4900 5547
Size: 4900 647
EaeI y'GGCC_r
Cuts at: 0 59 2753 4245 5547
Size: 59 2694 1492 1302
Fragments arranged by size:
2694 1492 1302 59
EarI CTCTTCn'nnn_
Cuts at: 0 1191 2594 5206 5547
Size: 1191 1403 2612 341
Fragments arranged by size:
2612 1403 1191 341
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 332 686 811 908 1202 1576 2023
Size: 67 265 354 125 97 294 374 447
Cuts at: 2023 2210 2618 2930 3001 3163 3206 3672 4139
Size: 187 408 312 71 162 43 466 467
Cuts at: 4139 4658 5179 5547
Size: 519 521 368
Fragments arranged by size:
521 519 467 466 447 408 374 368
354 312 294 265 187 162 125 97
71 67 43
EcoO109I rG'GnC_Cy
Cuts at: 0 417 418 789 1222 1402 2079 5547
Size: 417 1 371 433 180 677 3468
Fragments arranged by size:
3468 677 433 417 371 180 1
EcoRI G'AATT_C
Cuts at: 0 1121 5547
Size: 1121 4426
EcoRV GAT'ATC
Cuts at: 0 2645 4587 5507 5547
Size: 2645 1942 920 40
Fragments arranged by size:
2645 1942 920 40
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 808 1284 2191 3253 3694 3723 5547
Size: 258 550 476 907 1062 441 29 1824
Fragments arranged by size:
1824 1062 907 550 476 441 258 29
FspI TGC'GCA
Cuts at: 0 100 5194 5547
Size: 100 5094 353
Fragments arranged by size:
5094 353 100
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 59 709 741 747 754 779 786
Size: 27 32 650 32 6 7 25 7
Cuts at: 786 1162 1166 1194 1198 1321 1353 1869 1901
Size: 376 4 28 4 123 32 516 32
Cuts at: 1901 1996 2028 2432 2464 2593 2625 2933 2965
Size: 95 32 404 32 129 32 308 32
Cuts at: 2965 4678 4710 5547
Size: 1713 32 837
Fragments arranged by size:
1713 837 650 516 404 376 308 129
123 95 32 32 32 32 32 32
32 32 32 28 27 25 7 7
6 4 4
HincII GTy'rAC
Cuts at: 0 2009 2501 4619 5547
Size: 2009 492 2118 928
Fragments arranged by size:
2118 2009 928 492
HindIII A'AGCT_T
Cuts at: 0 104 5547
Size: 104 5443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1002 3140 3270 3772 5117 5547
Size: 1002 2138 130 502 1345 430
Fragments arranged by size:
2138 1345 1002 502 430 130
MscI TGG'CCA
Cuts at: 0 2755 4247 5547
Size: 2755 1492 1300
Fragments arranged by size:
2755 1492 1300
MslI CAynn'nnrTG
Cuts at: 0 598 806 959 2560 3104 4018 4253 5547
Size: 598 208 153 1601 544 914 235 1294
Fragments arranged by size:
1601 1294 914 598 544 235 208 153
MspA1I CmG'CkG
Cuts at: 0 96 444 910 2979 4872 5547
Size: 96 348 466 2069 1893 675
Fragments arranged by size:
2069 1893 675 466 348 96
NdeI CA'TA_TG
Cuts at: 0 2295 2994 4242 5547
Size: 2295 699 1248 1305
Fragments arranged by size:
2295 1305 1248 699
NsiI A_TGCA'T
Cuts at: 0 2295 2965 2994 5400 5547
Size: 2295 670 29 2406 147
Fragments arranged by size:
2406 2295 670 147 29
NspI r_CATG'y
Cuts at: 0 30 263 533 2833 2963 4612 5547
Size: 30 233 270 2300 130 1649 935
Fragments arranged by size:
2300 1649 935 270 233 130 30
PciI A'CATG_T
Cuts at: 0 26 529 5547
Size: 26 503 5018
Fragments arranged by size:
5018 503 26
PfoI T'CCnGG_A
Cuts at: 0 1125 3959 5547
Size: 1125 2834 1588
Fragments arranged by size:
2834 1588 1125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3820 3852 5547
Size: 3820 32 1695
Fragments arranged by size:
3820 1695 32
PpuMI rG'GwC_Cy
Cuts at: 0 789 1222 1402 2079 5547
Size: 789 433 180 677 3468
Fragments arranged by size:
3468 789 677 433 180
PspOMI G'GGCC_C
Cuts at: 0 417 5547
Size: 417 5130
PstI C_TGCA'G
Cuts at: 0 449 474 909 1394 4953 5275 5547
Size: 449 25 435 485 3559 322 272
Fragments arranged by size:
3559 485 449 435 322 272 25
PvuI CG_AT'CG
Cuts at: 0 2394 5547
Size: 2394 3153
PvuII CAG'CTG
Cuts at: 0 96 444 910 2979 4872 5547
Size: 96 348 466 2069 1893 675
Fragments arranged by size:
2069 1893 675 466 348 96
ScaI AGT'ACT
Cuts at: 0 238 4415 5547
Size: 238 4177 1132
Fragments arranged by size:
4177 1132 238
SexAI A'CCwGG_T
Cuts at: 0 1230 5547
Size: 1230 4317
SfcI C'TryA_G
Cuts at: 0 445 470 905 1079 1390 2034 3371 4706
Size: 445 25 435 174 311 644 1337 1335
Cuts at: 4706 4811 4949 5271 5547
Size: 105 138 322 276
Fragments arranged by size:
1337 1335 644 445 435 322 311 276
174 138 105 25
SmaI CCC'GGG
Cuts at: 0 40 5547
Size: 40 5507
SmlI C'TyrA_G
Cuts at: 0 1769 2237 3639 3708 3797 4994 5547
Size: 1769 468 1402 69 89 1197 553
Fragments arranged by size:
1769 1402 1197 553 468 89 69
SpeI A'CTAG_T
Cuts at: 0 1743 5547
Size: 1743 3804
SphI G_CATG'C
Cuts at: 0 263 2963 5547
Size: 263 2700 2584
Fragments arranged by size:
2700 2584 263
SspI AAT'ATT
Cuts at: 0 1107 3383 5547
Size: 1107 2276 2164
Fragments arranged by size:
2276 2164 1107
StyI C'CwwG_G
Cuts at: 0 2436 4732 5001 5547
Size: 2436 2296 269 546
Fragments arranged by size:
2436 2296 546 269
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2233 3494 3584 4122 5547
Size: 2233 1261 90 538 1425
Fragments arranged by size:
2233 1425 1261 538 90
TatI w'GTAC_w
Cuts at: 0 236 289 355 510 984 2038 4413 4425
Size: 236 53 66 155 474 1054 2375 12
Cuts at: 4425 5210 5547
Size: 785 337
Fragments arranged by size:
2375 1054 785 474 337 236 155 66
53 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2046 2078 3173 3205 3284 3316 5547
Size: 2046 32 1095 32 79 32 2231
Fragments arranged by size:
2231 2046 1095 79 32 32 32
XbaI T'CTAG_A
Cuts at: 0 297 2352 5547
Size: 297 2055 3195
Fragments arranged by size:
3195 2055 297
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1915 2847 5547
Size: 1915 932 2700
Fragments arranged by size:
2700 1915 932
XmaI C'CCGG_G
Cuts at: 0 38 5547
Size: 38 5509
XmnI GAAnn'nnTTC
Cuts at: 0 28 437 1106 1215 2360 3703 3832 4028
Size: 28 409 669 109 1145 1343 129 196
Cuts at: 4028 5547
Size: 1519
Fragments arranged by size:
1519 1343 1145 669 409 196 129 109
28
Enzymes that do cut:
AccI AcuI AflII AflIII AloI AlwNI ApaI ApoI
AseI AvaI BaeI BanI BanII BbsI BciVI BclI
BfrBI BlpI Bme1580I BplI BpmI BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsmI Bsp1286I
BspEI BspHI BsrDI BsrGI BssSI BstAPI BstXI BstYI
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
Hin4I HincII HindIII MmeI MscI MslI MspA1I NdeI
NsiI NspI PciI PfoI PpiI PpuMI PspOMI PstI
PvuI PvuII ScaI SexAI SfcI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI ApaLI AscI AsiSI AvrII BamHI BbeI BbvCI
BcgI BglI BglII BmgBI BmrI BmtI Bpu10I BsaAI
BsaBI BsiHKAI BsiWI BsmBI BspMI BsrBI BsrFI BssHII
BstBI BstEII BstZ17I BtgI EagI EciI EcoICRI EcoNI
FseI FspAI HaeII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PflMI PmeI PmlI PshAI PsiI PspXI PsrI
RsrII SacI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SnaBI SrfI StuI SwaI Tth111I
XhoI ZraI