Restriction map for
pF1KB4240
(Linear) (Six-base) MAPSORT of: KIBB4240.seq Check: 6102 from: 1 to: 1413
KIBB4240.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 429 448 1413
Size: 429 19 965
Fragments arranged by size:
965 429 19
AccI GT'mk_AC
Cuts at: 0 724 1413
Size: 724 689
Acc65I G'GTAC_C
Cuts at: 0 697 1413
Size: 697 716
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 629 961 1084 1206 1413
Size: 401 228 332 123 122 207
Fragments arranged by size:
401 332 228 207 123 122
AfeI AGC'GCT
Cuts at: 0 544 1413
Size: 544 869
AflIII A'CryG_T
Cuts at: 0 1095 1413
Size: 1095 318
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1360 1413
Size: 1360 53
ApoI r'AATT_y
Cuts at: 0 612 1413
Size: 612 801
BanI G'GyrC_C
Cuts at: 0 30 166 193 697 742 843 1413
Size: 30 136 27 504 45 101 570
Fragments arranged by size:
570 504 136 101 45 30 27
BanII G_rGCy'C
Cuts at: 0 535 1413
Size: 535 878
BbvCI CC'TCA_GC
Cuts at: 0 45 240 299 1358 1376 1413
Size: 45 195 59 1059 18 37
Fragments arranged by size:
1059 195 59 45 37 18
BglI GCCn_nnn'nGGC
Cuts at: 0 1372 1413
Size: 1372 41
BlpI GC'TnA_GC
Cuts at: 0 1400 1413
Size: 1400 13
Bme1580I G_kGCm'C
Cuts at: 0 196 1413
Size: 196 1217
BmgBI CAC'GTC
Cuts at: 0 1096 1413
Size: 1096 317
BmrI ACTGGGnnnn_n'
Cuts at: 0 476 1413
Size: 476 937
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 255 1413
Size: 255 1158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1202 1413
Size: 1202 211
Bpu10I CC'TnA_GC
Cuts at: 0 45 240 299 1033 1358 1376 1413
Size: 45 195 59 734 325 18 37
Fragments arranged by size:
734 325 195 59 45 37 18
BsaAI yAC'GTr
Cuts at: 0 874 1413
Size: 874 539
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 277 569 869 1314 1346 1413
Size: 277 292 300 445 32 67
Fragments arranged by size:
445 300 292 277 67 32
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1376 1413
Size: 53 1323 37
Fragments arranged by size:
1323 53 37
Bsp1286I G_dGCh'C
Cuts at: 0 196 535 1413
Size: 196 339 878
Fragments arranged by size:
878 339 196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 429 448 993 1352 1413
Size: 429 19 545 359 61
Fragments arranged by size:
545 429 359 61 19
BsrDI GCAATG_nn'
Cuts at: 0 1323 1413
Size: 1323 90
BstYI r'GATC_y
Cuts at: 0 1285 1413
Size: 1285 128
BtgI C'CryG_G
Cuts at: 0 1251 1413
Size: 1251 162
BtsI GCAGTG_nn'
Cuts at: 0 293 928 1385 1413
Size: 293 635 457 28
Fragments arranged by size:
635 457 293 28
DraI TTT'AAA
Cuts at: 0 492 1413
Size: 492 921
DrdI GACnn_nn'nnGTC
Cuts at: 0 663 1413
Size: 663 750
EarI CTCTTCn'nnn_
Cuts at: 0 155 552 645 685 1413
Size: 155 397 93 40 728
Fragments arranged by size:
728 397 155 93 40
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 119 669 1063 1106 1413
Size: 119 550 394 43 307
Fragments arranged by size:
550 394 307 119 43
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 629 961 1084 1202 1206 1413
Size: 401 228 332 123 118 4 207
Fragments arranged by size:
401 332 228 207 123 118 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 584 1413
Size: 584 829
EcoO109I rG'GnC_Cy
Cuts at: 0 803 1413
Size: 803 610
EcoRI G'AATT_C
Cuts at: 0 612 1413
Size: 612 801
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 776 1236 1413
Size: 776 460 177
Fragments arranged by size:
776 460 177
HaeII r_GCGC'y
Cuts at: 0 134 546 1413
Size: 134 412 867
Fragments arranged by size:
867 412 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 81 113 169 201 253 285 348 380
Size: 81 32 56 32 52 32 63 32
Cuts at: 380 496 528 1413
Size: 116 32 885
Fragments arranged by size:
885 116 81 63 56 52 32 32
32 32 32
KpnI G_GTAC'C
Cuts at: 0 701 1413
Size: 701 712
MslI CAynn'nnrTG
Cuts at: 0 841 1050 1413
Size: 841 209 363
Fragments arranged by size:
841 363 209
MspA1I CmG'CkG
Cuts at: 0 244 379 1413
Size: 244 135 1034
Fragments arranged by size:
1034 244 135
NcoI C'CATG_G
Cuts at: 0 1251 1413
Size: 1251 162
NspI r_CATG'y
Cuts at: 0 1234 1413
Size: 1234 179
PasI CC'CwG_GG
Cuts at: 0 718 1413
Size: 718 695
PfoI T'CCnGG_A
Cuts at: 0 780 1413
Size: 780 633
PpuMI rG'GwC_Cy
Cuts at: 0 803 1413
Size: 803 610
PstI C_TGCA'G
Cuts at: 0 363 1413
Size: 363 1050
PvuII CAG'CTG
Cuts at: 0 244 379 1413
Size: 244 135 1034
Fragments arranged by size:
1034 244 135
SapI GCTCTTCn'nnn_
Cuts at: 0 552 1413
Size: 552 861
SexAI A'CCwGG_T
Cuts at: 0 661 1413
Size: 661 752
SfcI C'TryA_G
Cuts at: 0 305 359 1413
Size: 305 54 1054
Fragments arranged by size:
1054 305 54
SnaBI TAC'GTA
Cuts at: 0 874 1413
Size: 874 539
SphI G_CATG'C
Cuts at: 0 1234 1413
Size: 1234 179
StyI C'CwwG_G
Cuts at: 0 1251 1413
Size: 1251 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 723 1413
Size: 723 690
Tth111I GACn'n_nGTC
Cuts at: 0 508 1413
Size: 508 905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1084 1147 1413
Size: 1084 63 266
Fragments arranged by size:
1084 266 63
XmnI GAAnn'nnTTC
Cuts at: 0 616 1413
Size: 616 797
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlfI ApoI
BanI BanII BbvCI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaAI BseRI BsgI Bsp1286I BspMI
BsrDI BstYI BtgI BtsI DraI DrdI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KpnI
MslI MspA1I NcoI NspI PasI PfoI PpuMI PstI
PvuII SapI SexAI SfcI SnaBI SphI StyI TaqII
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AloI AlwNI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BfrBI BglII
BmtI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseYI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraIII EaeI EagI
EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SpeI SrfI
SspI StuI SwaI TatI TstI XbaI XhoI XmaI
ZraI