Restriction map for
pF1KB4264
(Linear) (Six-base) MAPSORT of: KIBB4264.seq Check: 3002 from: 1 to: 2133
KIBB4264.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 0 795 829 2133
Size: 795 34 1304
Fragments arranged by size:
1304 795 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 737 791 1117 1454 1795 2133
Size: 137 600 54 326 337 341 338
Fragments arranged by size:
600 341 338 337 326 137 54
AflII C'TTAA_G
Cuts at: 0 7 634 2133
Size: 7 627 1499
Fragments arranged by size:
1499 627 7
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 522 1272 2133
Size: 522 750 861
Fragments arranged by size:
861 750 522
AlwNI CAG_nnn'CTG
Cuts at: 0 1432 2133
Size: 1432 701
ApaI G_GGCC'C
Cuts at: 0 1332 2133
Size: 1332 801
ApoI r'AATT_y
Cuts at: 0 1139 1672 1955 1997 2133
Size: 1139 533 283 42 136
Fragments arranged by size:
1139 533 283 136 42
AseI AT'TA_AT
Cuts at: 0 1526 2133
Size: 1526 607
BanII G_rGCy'C
Cuts at: 0 1332 2133
Size: 1332 801
BbvCI CC'TCA_GC
Cuts at: 0 2086 2133
Size: 2086 47
BciVI GTATCCnnnnn_n'
Cuts at: 0 270 2133
Size: 270 1863
BclI T'GATC_A
Cuts at: 0 1821 1835 2133
Size: 1821 14 298
Fragments arranged by size:
1821 298 14
BfrBI ATG'CAT
Cuts at: 0 870 2133
Size: 870 1263
BglI GCCn_nnn'nGGC
Cuts at: 0 1385 2133
Size: 1385 748
BglII A'GATC_T
Cuts at: 0 1716 2133
Size: 1716 417
BlpI GC'TnA_GC
Cuts at: 0 143 2133
Size: 143 1990
Bme1580I G_kGCm'C
Cuts at: 0 72 1332 2133
Size: 72 1260 801
Fragments arranged by size:
1260 801 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 442 934 2133
Size: 442 492 1199
Fragments arranged by size:
1199 492 442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 1343 2133
Size: 242 1101 790
Fragments arranged by size:
1101 790 242
Bpu10I CC'TnA_GC
Cuts at: 0 2086 2133
Size: 2086 47
BsaI GGTCTCn'nnnn_
Cuts at: 0 466 2133
Size: 466 1667
BsaWI w'CCGG_w
Cuts at: 0 1122 2133
Size: 1122 1011
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1289 1319 1730 1760 2133
Size: 1289 30 411 30 373
Fragments arranged by size:
1289 411 373 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1331 2133
Size: 1331 802
BseYI C'CCAG_C
Cuts at: 0 1293 1420 2133
Size: 1293 127 713
Fragments arranged by size:
1293 713 127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1754 2133
Size: 413 1341 379
Fragments arranged by size:
1341 413 379
BsiHKAI G_wGCw'C
Cuts at: 0 23 1470 2133
Size: 23 1447 663
Fragments arranged by size:
1447 663 23
BsmI GAATG_Cn'
Cuts at: 0 782 2133
Size: 782 1351
Bsp1286I G_dGCh'C
Cuts at: 0 23 72 1332 1470 2133
Size: 23 49 1260 138 663
Fragments arranged by size:
1260 663 138 49 23
BspEI T'CCGG_A
Cuts at: 0 1122 2133
Size: 1122 1011
BspHI T'CATG_A
Cuts at: 0 1358 2133
Size: 1358 775
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 514 2133
Size: 514 1619
BsrDI GCAATG_nn'
Cuts at: 0 455 2133
Size: 455 1678
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1110 1396 1684 1983 2030 2133
Size: 1110 286 288 299 47 103
Fragments arranged by size:
1110 299 288 286 103 47
BstYI r'GATC_y
Cuts at: 0 760 1716 2133
Size: 760 956 417
Fragments arranged by size:
956 760 417
BstZ17I GTA'TAC
Cuts at: 0 830 2133
Size: 830 1303
Bsu36I CC'TnA_GG
Cuts at: 0 854 2133
Size: 854 1279
BtgI C'CryG_G
Cuts at: 0 85 1580 2029 2133
Size: 85 1495 449 104
Fragments arranged by size:
1495 449 104 85
BtsI GCAGTG_nn'
Cuts at: 0 425 2133
Size: 425 1708
DraI TTT'AAA
Cuts at: 0 1275 1593 2133
Size: 1275 318 540
Fragments arranged by size:
1275 540 318
EaeI y'GGCC_r
Cuts at: 0 815 846 1386 2133
Size: 815 31 540 747
Fragments arranged by size:
815 747 540 31
EarI CTCTTCn'nnn_
Cuts at: 0 746 1595 2133
Size: 746 849 538
Fragments arranged by size:
849 746 538
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 242 737 791 1117 1343 1454 1795
Size: 137 105 495 54 326 226 111 341
Cuts at: 1795 2133
Size: 338
Fragments arranged by size:
495 341 338 326 226 137 111 105
54
EcoO109I rG'GnC_Cy
Cuts at: 0 1287 1328 1329 2133
Size: 1287 41 1 804
Fragments arranged by size:
1287 804 41 1
EcoRI G'AATT_C
Cuts at: 0 1672 2133
Size: 1672 461
EcoRV GAT'ATC
Cuts at: 0 654 992 1914 2133
Size: 654 338 922 219
Fragments arranged by size:
922 654 338 219
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 537 2133
Size: 537 1596
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 398 745 777 855 887 1289 1321
Size: 366 32 347 32 78 32 402 32
Cuts at: 1321 1538 1559 1570 1591 1689 1721 1732 1764
Size: 217 21 11 21 98 32 11 32
Cuts at: 1764 2133
Size: 369
Fragments arranged by size:
402 369 366 347 217 98 78 32
32 32 32 32 32 21 21 11
11
HincII GTy'rAC
Cuts at: 0 1868 2133
Size: 1868 265
MfeI C'AATT_G
Cuts at: 0 1382 2133
Size: 1382 751
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1443 2133
Size: 1443 690
MscI TGG'CCA
Cuts at: 0 817 1388 2133
Size: 817 571 745
Fragments arranged by size:
817 745 571
MslI CAynn'nnrTG
Cuts at: 0 679 823 1108 1579 1682 2133
Size: 679 144 285 471 103 451
Fragments arranged by size:
679 471 451 285 144 103
NcoI C'CATG_G
Cuts at: 0 85 1580 2029 2133
Size: 85 1495 449 104
Fragments arranged by size:
1495 449 104 85
NsiI A_TGCA'T
Cuts at: 0 872 2133
Size: 872 1261
NspI r_CATG'y
Cuts at: 0 741 2133
Size: 741 1392
PsiI TTA'TAA
Cuts at: 0 368 1145 2133
Size: 368 777 988
Fragments arranged by size:
988 777 368
PspOMI G'GGCC_C
Cuts at: 0 1328 2133
Size: 1328 805
PstI C_TGCA'G
Cuts at: 0 1015 2133
Size: 1015 1118
SfcI C'TryA_G
Cuts at: 0 1011 2133
Size: 1011 1122
SmlI C'TyrA_G
Cuts at: 0 7 634 2133
Size: 7 627 1499
Fragments arranged by size:
1499 627 7
SpeI A'CTAG_T
Cuts at: 0 832 2133
Size: 832 1301
SphI G_CATG'C
Cuts at: 0 741 2133
Size: 741 1392
StyI C'CwwG_G
Cuts at: 0 85 285 1580 2029 2133
Size: 85 200 1295 449 104
Fragments arranged by size:
1295 449 200 104 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 945 2133
Size: 945 1188
TatI w'GTAC_w
Cuts at: 0 515 1087 1092 2133
Size: 515 572 5 1041
Fragments arranged by size:
1041 572 515 5
Enzymes that do cut:
AccI AcuI AflII AlfI AlwNI ApaI ApoI AseI
BanII BbvCI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrDI BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraI
EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I
HincII MfeI MmeI MscI MslI NcoI NsiI NspI
PsiI PspOMI PstI SfcI SmlI SpeI SphI StyI
TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BcgI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI