(Linear) (Six-base) MAPSORT of: KIBB4277.seq  Check: 902  from: 1  to: 1275



KIBB4277.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     572     842    1262    1275 
   Size:        572     270     420      13

  Fragments arranged by size: 

                 572     420     270      13
 
AflII C'TTAA_G

Cuts at:      0     922    1275 
   Size:        922     353
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1027    1275 
   Size:       1027     248
 
AlwNI CAG_nnn'CTG

Cuts at:      0     739    1275 
   Size:        739     536
 
ApaI G_GGCC'C

Cuts at:      0     390    1275 
   Size:        390     885
 
ApoI r'AATT_y

Cuts at:      0     310    1275 
   Size:        310     965
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     396     429    1275 
   Size:        396      33     846

  Fragments arranged by size: 

                 846     396      33
 
BamHI G'GATC_C

Cuts at:      0    1254    1275 
   Size:       1254      21
 
BanII G_rGCy'C

Cuts at:      0     390    1275 
   Size:        390     885
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     664    1085    1190    1241    1275 
   Size:        664     421     105      51      34

  Fragments arranged by size: 

                 664     421     105      51      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     759    1275 
   Size:        759     516
 
BclI T'GATC_A

Cuts at:      0      47     744    1275 
   Size:         47     697     531

  Fragments arranged by size: 

                 697     531      47
 
Bme1580I G_kGCm'C

Cuts at:      0     390     575    1275 
   Size:        390     185     700

  Fragments arranged by size: 

                 700     390     185
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     135    1275 
   Size:        135    1140
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     512    1275 
   Size:        139     373     763

  Fragments arranged by size: 

                 763     373     139
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     414     444    1275 
   Size:        414      30     831

  Fragments arranged by size: 

                 831     414      30
 
BseYI C'CCAG_C

Cuts at:      0     127    1275 
   Size:        127    1148
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     271    1275 
   Size:        271    1004
 
BsmI GAATG_Cn'

Cuts at:      0     185     930    1275 
   Size:        185     745     345

  Fragments arranged by size: 

                 745     345     185
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     520    1275 
   Size:        520     755
 
Bsp1286I G_dGCh'C

Cuts at:      0     390     575    1275 
   Size:        390     185     700

  Fragments arranged by size: 

                 700     390     185
 
BstEII G'GTnAC_C

Cuts at:      0     974    1275 
   Size:        974     301
 
BstYI r'GATC_y

Cuts at:      0     483     903    1254    1275 
   Size:        483     420     351      21

  Fragments arranged by size: 

                 483     420     351      21
 
BtgI C'CryG_G

Cuts at:      0     519    1275 
   Size:        519     756
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     402    1275 
   Size:        402     873
 
BtsI GCAGTG_nn'

Cuts at:      0      38     815    1275 
   Size:         38     777     460

  Fragments arranged by size: 

                 777     460      38
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1076    1111    1275 
   Size:       1076      35     164

  Fragments arranged by size: 

                1076     164      35
 
EaeI y'GGCC_r

Cuts at:      0       2    1275 
   Size:          2    1273
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     139     512     572     842    1262    1275 
   Size:        139     373      60     270     420      13

  Fragments arranged by size: 

                 420     373     270     139      60      13
 
EcoO109I rG'GnC_Cy

Cuts at:      0     386    1275 
   Size:        386     889
 
EcoRV GAT'ATC

Cuts at:      0    1130    1275 
   Size:       1130     145
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     681    1275 
   Size:        681     594
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      99     131    1013    1045    1077    1275 
   Size:         99      32     882      32      32     198

  Fragments arranged by size: 

                 882     198      99      32      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     950    1198    1275 
   Size:        950     248      77

  Fragments arranged by size: 

                 950     248      77
 
MspA1I CmG'CkG

Cuts at:      0     880    1275 
   Size:        880     395
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     117    1275 
   Size:        117    1158
 
PfoI T'CCnGG_A

Cuts at:      0    1141    1275 
   Size:       1141     134
 
PshAI GACnn'nnGTC

Cuts at:      0     512    1269    1275 
   Size:        512     757       6

  Fragments arranged by size: 

                 757     512       6
 
PsiI TTA'TAA

Cuts at:      0    1226    1275 
   Size:       1226      49
 
PspOMI G'GGCC_C

Cuts at:      0     386    1275 
   Size:        386     889
 
PvuII CAG'CTG

Cuts at:      0     880    1275 
   Size:        880     395
 
SmlI C'TyrA_G

Cuts at:      0     922    1275 
   Size:        922     353
 
SspI AAT'ATT

Cuts at:      0     959    1275 
   Size:        959     316
 
StyI C'CwwG_G

Cuts at:      0    1159    1275 
   Size:       1159     116
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1152    1275 
   Size:       1152     123
 
XmnI GAAnn'nnTTC

Cuts at:      0     178    1275 
   Size:        178    1097
 

 Enzymes that do cut:

     AcuI    AflII     AhdI    AlwNI     ApaI     ApoI     BaeI    BamHI
    BanII     BbsI    BciVI     BclI Bme1580I     BmrI     BpmI    BsaXI
    BseYI     BsgI     BsmI    BsmBI Bsp1286I   BstEII    BstYI     BtgI
    BtgZI     BtsI    CspCI     EaeI  Eco57MI EcoO109I    EcoRV     FalI
    Hin4I     MslI   MspA1I    PflMI     PfoI    PshAI     PsiI   PspOMI
    PvuII     SmlI     SspI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BanI     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI