Restriction map for
pF1KB4280
(Linear) (Six-base) MAPSORT of: KIBB4280.seq Check: 4209 from: 1 to: 813
KIBB4280.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 587 813
Size: 587 226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 813
Size: 547 266
AflIII A'CryG_T
Cuts at: 0 693 813
Size: 693 120
AgeI A'CCGG_T
Cuts at: 0 584 813
Size: 584 229
AleI CACnn'nnGTG
Cuts at: 0 490 813
Size: 490 323
ApaI G_GGCC'C
Cuts at: 0 793 812 813
Size: 793 19 1
Fragments arranged by size:
793 19 1
ApaLI G'TGCA_C
Cuts at: 0 720 813
Size: 720 93
AvaI C'yCGr_G
Cuts at: 0 205 813
Size: 205 608
BanI G'GyrC_C
Cuts at: 0 587 813
Size: 587 226
BanII G_rGCy'C
Cuts at: 0 206 793 812 813
Size: 206 587 19 1
Fragments arranged by size:
587 206 19 1
BbsI GAAGACnn'nnnn_
Cuts at: 0 555 813
Size: 555 258
BglI GCCn_nnn'nGGC
Cuts at: 0 107 813
Size: 107 706
Bme1580I G_kGCm'C
Cuts at: 0 724 793 812 813
Size: 724 69 19 1
Fragments arranged by size:
724 69 19 1
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 813
Size: 93 720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 358 790 813
Size: 247 111 432 23
Fragments arranged by size:
432 247 111 23
BsaAI yAC'GTr
Cuts at: 0 696 813
Size: 696 117
BsaHI Gr'CG_yC
Cuts at: 0 302 813
Size: 302 511
BsaWI w'CCGG_w
Cuts at: 0 584 813
Size: 584 229
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 813
Size: 310 30 473
Fragments arranged by size:
473 310 30
BseYI C'CCAG_C
Cuts at: 0 39 89 342 446 521 813
Size: 39 50 253 104 75 292
Fragments arranged by size:
292 253 104 75 50 39
BsiHKAI G_wGCw'C
Cuts at: 0 320 724 813
Size: 320 404 89
Fragments arranged by size:
404 320 89
Bsp1286I G_dGCh'C
Cuts at: 0 206 320 724 793 812 813
Size: 206 114 404 69 19 1
Fragments arranged by size:
404 206 114 69 19 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 152 599 813
Size: 152 447 214
Fragments arranged by size:
447 214 152
BsrFI r'CCGG_y
Cuts at: 0 584 813
Size: 584 229
BstEII G'GTnAC_C
Cuts at: 0 483 813
Size: 483 330
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 813
Size: 273 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 358 547 790 813
Size: 247 111 189 243 23
Fragments arranged by size:
247 243 189 111 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 772 813
Size: 772 41
EcoO109I rG'GnC_Cy
Cuts at: 0 9 93 347 789 808 813
Size: 9 84 254 442 19 5
Fragments arranged by size:
442 254 84 19 9 5
FspI TGC'GCA
Cuts at: 0 373 813
Size: 373 440
FspAI rTGC'GCAy
Cuts at: 0 373 813
Size: 373 440
KpnI G_GTAC'C
Cuts at: 0 591 813
Size: 591 222
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 477 813
Size: 477 336
MslI CAynn'nnrTG
Cuts at: 0 271 490 813
Size: 271 219 323
Fragments arranged by size:
323 271 219
MspA1I CmG'CkG
Cuts at: 0 31 367 450 525 710 813
Size: 31 336 83 75 185 103
Fragments arranged by size:
336 185 103 83 75 31
NspI r_CATG'y
Cuts at: 0 199 813
Size: 199 614
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 813
Size: 453 360
PfoI T'CCnGG_A
Cuts at: 0 740 813
Size: 740 73
PmlI CAC'GTG
Cuts at: 0 696 813
Size: 696 117
PspOMI G'GGCC_C
Cuts at: 0 789 808 813
Size: 789 19 5
Fragments arranged by size:
789 19 5
PstI C_TGCA'G
Cuts at: 0 131 194 813
Size: 131 63 619
Fragments arranged by size:
619 131 63
PvuII CAG'CTG
Cuts at: 0 31 367 525 710 813
Size: 31 336 158 185 103
Fragments arranged by size:
336 185 158 103 31
SfcI C'TryA_G
Cuts at: 0 127 190 813
Size: 127 63 623
Fragments arranged by size:
623 127 63
SmaI CCC'GGG
Cuts at: 0 207 813
Size: 207 606
SphI G_CATG'C
Cuts at: 0 199 813
Size: 199 614
StyI C'CwwG_G
Cuts at: 0 161 813
Size: 161 652
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 142 460 813
Size: 142 318 353
Fragments arranged by size:
353 318 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 813
Size: 310 32 471
Fragments arranged by size:
471 310 32
Tth111I GACn'n_nGTC
Cuts at: 0 481 559 813
Size: 481 78 254
Fragments arranged by size:
481 254 78
XmaI C'CCGG_G
Cuts at: 0 205 813
Size: 205 608
XmnI GAAnn'nnTTC
Cuts at: 0 118 813
Size: 118 695
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AleI ApaI ApaLI AvaI
BanI BanII BbsI BglI Bme1580I BmrI BpmI BsaAI
BsaHI BsaWI BsaXI BseYI BsiHKAI Bsp1286I BspMI BsrFI
BstEII BstXI Eco57MI EcoNI EcoO109I FspI FspAI KpnI
MmeI MslI MspA1I NspI PflMI PfoI PmlI PspOMI
PstI PvuII SfcI SmaI SphI StyI TaqII TstI
Tth111I XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AlfI
AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoRI
EcoRV FalI FseI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TatI XbaI XcmI XhoI
ZraI