(Linear) (Six-base) MAPSORT of: KIBB4295.seq  Check: 2083  from: 1  to: 711



KIBB4295.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

Acc65I G'GTAC_C

Cuts at:      0     619     711 
   Size:        619      92
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     711 
   Size:        493     218
 
ApoI r'AATT_y

Cuts at:      0     149     208     612     711 
   Size:        149      59     404      99

  Fragments arranged by size: 

                 404     149      99      59
 
BamHI G'GATC_C

Cuts at:      0     537     711 
   Size:        537     174
 
BanI G'GyrC_C

Cuts at:      0     619     711 
   Size:        619      92
 
BanII G_rGCy'C

Cuts at:      0     246     711 
   Size:        246     465
 
BbvCI CC'TCA_GC

Cuts at:      0     407     533     711 
   Size:        407     126     178

  Fragments arranged by size: 

                 407     178     126
 
BglII A'GATC_T

Cuts at:      0     462     711 
   Size:        462     249
 
Bpu10I CC'TnA_GC

Cuts at:      0     407     533     711 
   Size:        407     126     178

  Fragments arranged by size: 

                 407     178     126
 
BsaWI w'CCGG_w

Cuts at:      0     604     711 
   Size:        604     107
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     254     711 
   Size:        254     457
 
BsiHKAI G_wGCw'C

Cuts at:      0     260     711 
   Size:        260     451
 
BsmI GAATG_Cn'

Cuts at:      0     552     711 
   Size:        552     159
 
Bsp1286I G_dGCh'C

Cuts at:      0     246     260     711 
   Size:        246      14     451

  Fragments arranged by size: 

                 451     246      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     187     711 
   Size:        187     524
 
BssSI C'ACGA_G

Cuts at:      0     259     711 
   Size:        259     452
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      33     711 
   Size:         33     678
 
BstYI r'GATC_y

Cuts at:      0     462     537     711 
   Size:        462      75     174

  Fragments arranged by size: 

                 462     174      75
 
BtsI GCAGTG_nn'

Cuts at:      0     191     711 
   Size:        191     520
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     711 
   Size:        493     218
 
EcoRI G'AATT_C

Cuts at:      0     612     711 
   Size:        612      99
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     268     300     626     658     711 
   Size:        268      32     326      32      53

  Fragments arranged by size: 

                 326     268      53      32      32
 
KpnI G_GTAC'C

Cuts at:      0     623     711 
   Size:        623      88
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     286     711 
   Size:         39     247     425

  Fragments arranged by size: 

                 425     247      39
 
MslI CAynn'nnrTG

Cuts at:      0      31     711 
   Size:         31     680
 
MspA1I CmG'CkG

Cuts at:      0     698     711 
   Size:        698      13
 
PstI C_TGCA'G

Cuts at:      0     198     711 
   Size:        198     513
 
PvuII CAG'CTG

Cuts at:      0     698     711 
   Size:        698      13
 
SfcI C'TryA_G

Cuts at:      0     194     441     699     711 
   Size:        194     247     258      12

  Fragments arranged by size: 

                 258     247     194      12
 
StuI AGG'CCT

Cuts at:      0     229     711 
   Size:        229     482
 
TatI w'GTAC_w

Cuts at:      0     348     711 
   Size:        348     363
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      33     711 
   Size:         33     678
 

 Enzymes that do cut:

   Acc65I     AcuI     ApoI    BamHI     BanI    BanII    BbvCI    BglII
   Bpu10I    BsaWI    BseRI  BsiHKAI     BsmI Bsp1286I    BspMI    BssSI
    BstXI    BstYI     BtsI  Eco57MI    EcoRI    Hin4I     KpnI     MmeI
     MslI   MspA1I     PstI    PvuII     SfcI     StuI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI