Restriction map for
pF1KB4303
(Linear) (Six-base) MAPSORT of: KIBB4303.seq Check: 1508 from: 1 to: 1905
KIBB4303.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1214 1738 1905
Size: 1214 524 167
Fragments arranged by size:
1214 524 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 373 1382 1905
Size: 271 102 1009 523
Fragments arranged by size:
1009 523 271 102
AflII C'TTAA_G
Cuts at: 0 211 1905
Size: 211 1694
AflIII A'CryG_T
Cuts at: 0 392 1308 1560 1905
Size: 392 916 252 345
Fragments arranged by size:
916 392 345 252
AhdI GACnn_n'nnGTC
Cuts at: 0 741 1905
Size: 741 1164
AlwNI CAG_nnn'CTG
Cuts at: 0 1381 1399 1467 1905
Size: 1381 18 68 438
Fragments arranged by size:
1381 438 68 18
ApaI G_GGCC'C
Cuts at: 0 800 1905
Size: 800 1105
ApaLI G'TGCA_C
Cuts at: 0 586 604 1905
Size: 586 18 1301
Fragments arranged by size:
1301 586 18
AvaI C'yCGr_G
Cuts at: 0 748 799 959 1637 1905
Size: 748 51 160 678 268
Fragments arranged by size:
748 678 268 160 51
AvrII C'CTAG_G
Cuts at: 0 73 1752 1905
Size: 73 1679 153
Fragments arranged by size:
1679 153 73
BamHI G'GATC_C
Cuts at: 0 1647 1905
Size: 1647 258
BanI G'GyrC_C
Cuts at: 0 727 1108 1870 1905
Size: 727 381 762 35
Fragments arranged by size:
762 727 381 35
BanII G_rGCy'C
Cuts at: 0 197 800 1151 1334 1427 1905
Size: 197 603 351 183 93 478
Fragments arranged by size:
603 478 351 197 183 93
BbeI G_GCGC'C
Cuts at: 0 1874 1905
Size: 1874 31
BbvCI CC'TCA_GC
Cuts at: 0 1400 1905
Size: 1400 505
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1905
Size: 541 1364
BlpI GC'TnA_GC
Cuts at: 0 508 1905
Size: 508 1397
Bme1580I G_kGCm'C
Cuts at: 0 147 590 608 730 800 1085 1695 1905
Size: 147 443 18 122 70 285 610 210
Fragments arranged by size:
610 443 285 210 147 122 70 18
BmgBI CAC'GTC
Cuts at: 0 393 1563 1905
Size: 393 1170 342
Fragments arranged by size:
1170 393 342
BmrI ACTGGGnnnn_n'
Cuts at: 0 1068 1625 1905
Size: 1068 557 280
Fragments arranged by size:
1068 557 280
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 1905
Size: 936 969
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1303 1348 1411 1459 1905
Size: 123 1180 45 63 48 446
Fragments arranged by size:
1180 446 123 63 48 45
Bpu10I CC'TnA_GC
Cuts at: 0 1400 1468 1905
Size: 1400 68 437
Fragments arranged by size:
1400 437 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1321 1905
Size: 667 654 584
Fragments arranged by size:
667 654 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 877 1072 1905
Size: 877 195 833
Fragments arranged by size:
877 833 195
BsaAI yAC'GTr
Cuts at: 0 483 603 1311 1905
Size: 483 120 708 594
Fragments arranged by size:
708 594 483 120
BsaHI Gr'CG_yC
Cuts at: 0 856 1871 1905
Size: 856 1015 34
Fragments arranged by size:
1015 856 34
BsaWI w'CCGG_w
Cuts at: 0 1028 1095 1905
Size: 1028 67 810
Fragments arranged by size:
1028 810 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1153 1183 1213 1243 1905
Size: 1153 30 30 30 662
Fragments arranged by size:
1153 662 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 967 1456 1459 1905
Size: 397 570 489 3 446
Fragments arranged by size:
570 489 446 397 3
BseYI C'CCAG_C
Cuts at: 0 186 819 1002 1321 1905
Size: 186 633 183 319 584
Fragments arranged by size:
633 584 319 186 183
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 1905
Size: 415 1490
BsiEI CG_ry'CG
Cuts at: 0 536 712 1675 1905
Size: 536 176 963 230
Fragments arranged by size:
963 536 230 176
BsiHKAI G_wGCw'C
Cuts at: 0 590 608 917 1427 1454 1905
Size: 590 18 309 510 27 451
Fragments arranged by size:
590 510 451 309 27 18
Bsp1286I G_dGCh'C
Cuts at: 0 147 197 590 608 730 800 917 1085
Size: 147 50 393 18 122 70 117 168
Cuts at: 1085 1151 1334 1427 1454 1695 1905
Size: 66 183 93 27 241 210
Fragments arranged by size:
393 241 210 183 168 147 122 117
93 70 66 50 27 18
BspEI T'CCGG_A
Cuts at: 0 1028 1905
Size: 1028 877
BspHI T'CATG_A
Cuts at: 0 332 1905
Size: 332 1573
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 503 1214 1738 1905
Size: 503 711 524 167
Fragments arranged by size:
711 524 503 167
BsrBI CCG'CTC
Cuts at: 0 525 1581 1905
Size: 525 1056 324
Fragments arranged by size:
1056 525 324
BsrFI r'CCGG_y
Cuts at: 0 410 590 1848 1905
Size: 410 180 1258 57
Fragments arranged by size:
1258 410 180 57
BssHII G'CGCG_C
Cuts at: 0 544 1905
Size: 544 1361
BssSI C'ACGA_G
Cuts at: 0 1807 1905
Size: 1807 98
BstBI TT'CG_AA
Cuts at: 0 1103 1905
Size: 1103 802
BstEII G'GTnAC_C
Cuts at: 0 154 1905
Size: 154 1751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1275 1281 1905
Size: 1275 6 624
Fragments arranged by size:
1275 624 6
BstYI r'GATC_y
Cuts at: 0 752 1647 1905
Size: 752 895 258
Fragments arranged by size:
895 752 258
Bsu36I CC'TnA_GG
Cuts at: 0 950 1243 1905
Size: 950 293 662
Fragments arranged by size:
950 662 293
BtgI C'CryG_G
Cuts at: 0 875 1157 1274 1643 1675 1905
Size: 875 282 117 369 32 230
Fragments arranged by size:
875 369 282 230 117 32
BtsI GCAGTG_nn'
Cuts at: 0 502 1151 1905
Size: 502 649 754
Fragments arranged by size:
754 649 502
DrdI GACnn_nn'nnGTC
Cuts at: 0 943 1255 1905
Size: 943 312 650
Fragments arranged by size:
943 650 312
EaeI y'GGCC_r
Cuts at: 0 533 572 648 662 1672 1850 1905
Size: 533 39 76 14 1010 178 55
Fragments arranged by size:
1010 533 178 76 55 39 14
EagI C'GGCC_G
Cuts at: 0 533 1672 1905
Size: 533 1139 233
Fragments arranged by size:
1139 533 233
EarI CTCTTCn'nnn_
Cuts at: 0 349 1905
Size: 349 1556
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 574 646 895 1905
Size: 574 72 249 1010
Fragments arranged by size:
1010 574 249 72
EcoICRI GAG'CTC
Cuts at: 0 1425 1905
Size: 1425 480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 271 373 1303 1348 1382 1411 1459
Size: 123 148 102 930 45 34 29 48
Cuts at: 1459 1905
Size: 446
Fragments arranged by size:
930 446 148 123 102 48 45 34
29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 782 1441 1767 1905
Size: 782 659 326 138
Fragments arranged by size:
782 659 326 138
EcoO109I rG'GnC_Cy
Cuts at: 0 492 945 1257 1264 1548 1626 1710 1748
Size: 492 453 312 7 284 78 84 38
Cuts at: 1748 1905
Size: 157
Fragments arranged by size:
492 453 312 284 157 84 78 38
7
HaeII r_GCGC'y
Cuts at: 0 820 1321 1776 1874 1905
Size: 820 501 455 98 31
Fragments arranged by size:
820 501 455 98 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 658 690 727 759 1153 1185 1905
Size: 658 32 37 32 394 32 720
Fragments arranged by size:
720 658 394 37 32 32 32
HincII GTy'rAC
Cuts at: 0 1519 1905
Size: 1519 386
KasI G'GCGC_C
Cuts at: 0 1870 1905
Size: 1870 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 1151 1905
Size: 246 905 754
Fragments arranged by size:
905 754 246
MscI TGG'CCA
Cuts at: 0 574 650 1905
Size: 574 76 1255
Fragments arranged by size:
1255 574 76
MslI CAynn'nnrTG
Cuts at: 0 202 1156 1273 1905
Size: 202 954 117 632
Fragments arranged by size:
954 632 202 117
MspA1I CmG'CkG
Cuts at: 0 190 429 644 930 975 1296 1360 1378
Size: 190 239 215 286 45 321 64 18
Cuts at: 1378 1615 1697 1905
Size: 237 82 208
Fragments arranged by size:
321 286 239 237 215 208 190 82
64 45 18
NaeI GCC'GGC
Cuts at: 0 1850 1905
Size: 1850 55
NarI GG'CG_CC
Cuts at: 0 1871 1905
Size: 1871 34
NcoI C'CATG_G
Cuts at: 0 1157 1274 1905
Size: 1157 117 631
Fragments arranged by size:
1157 631 117
NdeI CA'TA_TG
Cuts at: 0 1661 1905
Size: 1661 244
NgoMIV G'CCGG_C
Cuts at: 0 1848 1905
Size: 1848 57
NspI r_CATG'y
Cuts at: 0 351 1124 1905
Size: 351 773 781
Fragments arranged by size:
781 773 351
PasI CC'CwG_GG
Cuts at: 0 56 1820 1905
Size: 56 1764 85
Fragments arranged by size:
1764 85 56
PfoI T'CCnGG_A
Cuts at: 0 443 1070 1427 1905
Size: 443 627 357 478
Fragments arranged by size:
627 478 443 357
PmlI CAC'GTG
Cuts at: 0 1311 1905
Size: 1311 594
PpuMI rG'GwC_Cy
Cuts at: 0 492 945 1257 1548 1626 1748 1905
Size: 492 453 312 291 78 122 157
Fragments arranged by size:
492 453 312 291 157 122 78
PspOMI G'GGCC_C
Cuts at: 0 796 1905
Size: 796 1109
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 445 477 1905
Size: 445 32 1428
Fragments arranged by size:
1428 445 32
PstI C_TGCA'G
Cuts at: 0 230 761 1598 1905
Size: 230 531 837 307
Fragments arranged by size:
837 531 307 230
PvuII CAG'CTG
Cuts at: 0 429 1296 1360 1615 1905
Size: 429 867 64 255 290
Fragments arranged by size:
867 429 290 255 64
SacI G_AGCT'C
Cuts at: 0 1427 1905
Size: 1427 478
SanDI GG'GwC_CC
Cuts at: 0 945 1626 1748 1905
Size: 945 681 122 157
Fragments arranged by size:
945 681 157 122
SbfI CC_TGCA'GG
Cuts at: 0 761 1905
Size: 761 1144
SexAI A'CCwGG_T
Cuts at: 0 464 1905
Size: 464 1441
SfcI C'TryA_G
Cuts at: 0 226 757 1594 1905
Size: 226 531 837 311
Fragments arranged by size:
837 531 311 226
SfoI GGC'GCC
Cuts at: 0 1872 1905
Size: 1872 33
SmaI CCC'GGG
Cuts at: 0 1639 1905
Size: 1639 266
SmlI C'TyrA_G
Cuts at: 0 211 682 959 1300 1905
Size: 211 471 277 341 605
Fragments arranged by size:
605 471 341 277 211
StuI AGG'CCT
Cuts at: 0 1757 1905
Size: 1757 148
StyI C'CwwG_G
Cuts at: 0 73 1157 1274 1752 1905
Size: 73 1084 117 478 153
Fragments arranged by size:
1084 478 153 117 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 811 1287 1905
Size: 811 476 618
Fragments arranged by size:
811 618 476
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 400 432 1905
Size: 400 32 1473
Fragments arranged by size:
1473 400 32
Tth111I GACn'n_nGTC
Cuts at: 0 1167 1905
Size: 1167 738
XhoI C'TCGA_G
Cuts at: 0 959 1905
Size: 959 946
XmaI C'CCGG_G
Cuts at: 0 1637 1905
Size: 1637 268
Enzymes that do cut:
AarI AcuI AflII AflIII AhdI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbeI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BssHII BssSI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PasI PfoI PmlI PpuMI PspOMI PsrI PstI PvuII
SacI SanDI SbfI SexAI SfcI SfoI SmaI SmlI
StuI StyI TaqII TstI Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AleI AlfI
AloI ApoI AscI AseI AsiSI BaeI BbsI BcgI
BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI
BsmBI BsrDI BsrGI BstAPI BstZ17I BtgZI ClaI CspCI
DraI DraIII EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PpiI PshAI PsiI
PspXI PvuI RsrII SacII SalI SapI ScaI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI XbaI XcmI XmnI ZraI