Restriction map for
pF1KB4307
(Linear) (Six-base) MAPSORT of: KIBB4307.seq Check: 1960 from: 1 to: 1002
KIBB4307.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AatII G_ACGT'C
Cuts at: 0 539 1002
Size: 539 463
ApoI r'AATT_y
Cuts at: 0 41 412 891 1002
Size: 41 371 479 111
Fragments arranged by size:
479 371 111 41
BamHI G'GATC_C
Cuts at: 0 248 1002
Size: 248 754
BanII G_rGCy'C
Cuts at: 0 392 686 1002
Size: 392 294 316
Fragments arranged by size:
392 316 294
BciVI GTATCCnnnnn_n'
Cuts at: 0 390 691 1002
Size: 390 301 311
Fragments arranged by size:
390 311 301
BglI GCCn_nnn'nGGC
Cuts at: 0 225 1002
Size: 225 777
Bme1580I G_kGCm'C
Cuts at: 0 221 1002
Size: 221 781
BmrI ACTGGGnnnn_n'
Cuts at: 0 184 1002
Size: 184 818
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1002
Size: 178 824
BsaAI yAC'GTr
Cuts at: 0 216 1002
Size: 216 786
BsaHI Gr'CG_yC
Cuts at: 0 536 1002
Size: 536 466
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 829 1002
Size: 829 173
BseYI C'CCAG_C
Cuts at: 0 220 834 1002
Size: 220 614 168
Fragments arranged by size:
614 220 168
BsiHKAI G_wGCw'C
Cuts at: 0 392 686 1002
Size: 392 294 316
Fragments arranged by size:
392 316 294
BsiWI C'GTAC_G
Cuts at: 0 188 1002
Size: 188 814
BsmBI CGTCTCn'nnnn_
Cuts at: 0 527 1002
Size: 527 475
Bsp1286I G_dGCh'C
Cuts at: 0 221 392 686 1002
Size: 221 171 294 316
Fragments arranged by size:
316 294 221 171
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 631 1002
Size: 631 371
BstYI r'GATC_y
Cuts at: 0 248 377 1002
Size: 248 129 625
Fragments arranged by size:
625 248 129
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 806 1002
Size: 806 196
BtsI GCAGTG_nn'
Cuts at: 0 245 1002
Size: 245 757
EaeI y'GGCC_r
Cuts at: 0 749 1002
Size: 749 253
EcoICRI GAG'CTC
Cuts at: 0 390 684 1002
Size: 390 294 318
Fragments arranged by size:
390 318 294
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1002
Size: 178 824
EcoRI G'AATT_C
Cuts at: 0 891 1002
Size: 891 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 85 117 805 832 837 856 864 888
Size: 85 32 688 27 5 19 8 24
Cuts at: 888 958 990 1002
Size: 70 32 12
Fragments arranged by size:
688 85 70 32 32 27 24 19
12 8 5
HincII GTy'rAC
Cuts at: 0 864 1002
Size: 864 138
MfeI C'AATT_G
Cuts at: 0 632 1002
Size: 632 370
MscI TGG'CCA
Cuts at: 0 751 1002
Size: 751 251
MslI CAynn'nnrTG
Cuts at: 0 238 736 1002
Size: 238 498 266
Fragments arranged by size:
498 266 238
MspA1I CmG'CkG
Cuts at: 0 224 1002
Size: 224 778
PmlI CAC'GTG
Cuts at: 0 216 1002
Size: 216 786
PstI C_TGCA'G
Cuts at: 0 855 1002
Size: 855 147
SacI G_AGCT'C
Cuts at: 0 392 686 1002
Size: 392 294 316
Fragments arranged by size:
392 316 294
SfcI C'TryA_G
Cuts at: 0 851 1002
Size: 851 151
StyI C'CwwG_G
Cuts at: 0 104 1002
Size: 104 898
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 559 1002
Size: 559 443
TatI w'GTAC_w
Cuts at: 0 552 1002
Size: 552 450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 689 721 953 985 1002
Size: 689 32 232 32 17
Fragments arranged by size:
689 232 32 32 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1002
Size: 315 687
ZraI GAC'GTC
Cuts at: 0 537 1002
Size: 537 465
Enzymes that do cut:
AatII ApoI BamHI BanII BciVI BglI Bme1580I BmrI
BpmI BsaAI BsaHI BseRI BseYI BsiHKAI BsiWI BsmBI
Bsp1286I BspMI BstYI BtgZI BtsI EaeI EcoICRI Eco57MI
EcoRI Hin4I HincII MfeI MscI MslI MspA1I PmlI
PstI SacI SfcI StyI TaqII TatI TstI XcmI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BbsI BbvCI BcgI BclI BfrBI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BsgI BsiEI BsmI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XhoI XmaI XmnI