Restriction map for
pF1KB4325
(Linear) (Six-base) MAPSORT of: KIBB4325.seq Check: 4907 from: 1 to: 1308
KIBB4325.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 610 1308
Size: 610 698
AccI GT'mk_AC
Cuts at: 0 272 1308
Size: 272 1036
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 706 937 1132 1308
Size: 263 443 231 195 176
Fragments arranged by size:
443 263 231 195 176
AleI CACnn'nnGTG
Cuts at: 0 814 1123 1308
Size: 814 309 185
Fragments arranged by size:
814 309 185
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1282 1308
Size: 1282 26
AlwNI CAG_nnn'CTG
Cuts at: 0 340 1155 1308
Size: 340 815 153
Fragments arranged by size:
815 340 153
ApoI r'AATT_y
Cuts at: 0 282 897 947 1308
Size: 282 615 50 361
Fragments arranged by size:
615 361 282 50
BamHI G'GATC_C
Cuts at: 0 704 1308
Size: 704 604
BanI G'GyrC_C
Cuts at: 0 544 570 1249 1308
Size: 544 26 679 59
Fragments arranged by size:
679 544 59 26
BanII G_rGCy'C
Cuts at: 0 124 241 1085 1308
Size: 124 117 844 223
Fragments arranged by size:
844 223 124 117
BbsI GAAGACnn'nnnn_
Cuts at: 0 948 1308
Size: 948 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 801 835 1308
Size: 801 34 473
Fragments arranged by size:
801 473 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1177 1308
Size: 1177 131
BglII A'GATC_T
Cuts at: 0 741 1308
Size: 741 567
Bme1580I G_kGCm'C
Cuts at: 0 1254 1308
Size: 1254 54
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1308
Size: 409 899
BsaI GGTCTCn'nnnn_
Cuts at: 0 976 1308
Size: 976 332
BsaHI Gr'CG_yC
Cuts at: 0 577 791 1308
Size: 577 214 517
Fragments arranged by size:
577 517 214
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1091 1121 1282 1308
Size: 1091 30 161 26
Fragments arranged by size:
1091 161 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1252 1308
Size: 1252 56
BseYI C'CCAG_C
Cuts at: 0 518 716 1308
Size: 518 198 592
Fragments arranged by size:
592 518 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 1204 1308
Size: 532 672 104
Fragments arranged by size:
672 532 104
BsiEI CG_ry'CG
Cuts at: 0 650 1308
Size: 650 658
BsiHKAI G_wGCw'C
Cuts at: 0 1085 1308
Size: 1085 223
BsmI GAATG_Cn'
Cuts at: 0 377 1308
Size: 377 931
Bsp1286I G_dGCh'C
Cuts at: 0 124 241 1085 1254 1308
Size: 124 117 844 169 54
Fragments arranged by size:
844 169 124 117 54
BspHI T'CATG_A
Cuts at: 0 1193 1308
Size: 1193 115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 610 1179 1308
Size: 610 569 129
Fragments arranged by size:
610 569 129
BsrDI GCAATG_nn'
Cuts at: 0 437 1308
Size: 437 871
BsrFI r'CCGG_y
Cuts at: 0 573 1016 1308
Size: 573 443 292
Fragments arranged by size:
573 443 292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 51 1308
Size: 51 1257
BstYI r'GATC_y
Cuts at: 0 84 330 704 741 1308
Size: 84 246 374 37 567
Fragments arranged by size:
567 374 246 84 37
Bsu36I CC'TnA_GG
Cuts at: 0 557 1308
Size: 557 751
BtgI C'CryG_G
Cuts at: 0 605 1308
Size: 605 703
EaeI y'GGCC_r
Cuts at: 0 1018 1308
Size: 1018 290
EarI CTCTTCn'nnn_
Cuts at: 0 838 1308
Size: 838 470
EcoICRI GAG'CTC
Cuts at: 0 1083 1308
Size: 1083 225
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 706 937 1132 1308
Size: 263 443 231 195 176
Fragments arranged by size:
443 263 231 195 176
EcoRI G'AATT_C
Cuts at: 0 947 1308
Size: 947 361
FspI TGC'GCA
Cuts at: 0 376 1308
Size: 376 932
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 757 789 873 905 1091 1123 1204 1236
Size: 757 32 84 32 186 32 81 32
Cuts at: 1236 1308
Size: 72
Fragments arranged by size:
757 186 84 81 72 32 32 32
32
HincII GTy'rAC
Cuts at: 0 405 918 1308
Size: 405 513 390
Fragments arranged by size:
513 405 390
HpaI GTT'AAC
Cuts at: 0 405 1308
Size: 405 903
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1073 1308
Size: 944 129 235
Fragments arranged by size:
944 235 129
MslI CAynn'nnrTG
Cuts at: 0 761 814 1123 1135 1308
Size: 761 53 309 12 173
Fragments arranged by size:
761 309 173 53 12
MspA1I CmG'CkG
Cuts at: 0 1274 1308
Size: 1274 34
NaeI GCC'GGC
Cuts at: 0 575 1308
Size: 575 733
NcoI C'CATG_G
Cuts at: 0 605 1308
Size: 605 703
NgoMIV G'CCGG_C
Cuts at: 0 573 1308
Size: 573 735
NspI r_CATG'y
Cuts at: 0 808 1308
Size: 808 500
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1282 1308
Size: 1282 26
PstI C_TGCA'G
Cuts at: 0 430 590 1307 1308
Size: 430 160 717 1
Fragments arranged by size:
717 430 160 1
SacI G_AGCT'C
Cuts at: 0 1085 1308
Size: 1085 223
SapI GCTCTTCn'nnn_
Cuts at: 0 838 1308
Size: 838 470
SfcI C'TryA_G
Cuts at: 0 426 586 1303 1308
Size: 426 160 717 5
Fragments arranged by size:
717 426 160 5
SmlI C'TyrA_G
Cuts at: 0 388 1308
Size: 388 920
SphI G_CATG'C
Cuts at: 0 808 1308
Size: 808 500
StyI C'CwwG_G
Cuts at: 0 605 660 1308
Size: 605 55 648
Fragments arranged by size:
648 605 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 554 586 1308
Size: 554 32 722
Fragments arranged by size:
722 554 32
Tth111I GACn'n_nGTC
Cuts at: 0 684 1308
Size: 684 624
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 612 1308
Size: 612 696
XmnI GAAnn'nnTTC
Cuts at: 0 316 1308
Size: 316 992
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApoI BamHI
BanI BanII BbsI BcgI BglI BglII Bme1580I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI BstYI
Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoRI FspI
Hin4I HincII HpaI MmeI MslI MspA1I NaeI NcoI
NgoMIV NspI PpiI PstI SacI SapI SfcI SmlI
SphI StyI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BbvCI BciVI BclI BfrBI BlpI BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaWI
BsiWI BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstXI BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoNI EcoO109I EcoRV FalI
FseI FspAI HaeII HindIII KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TaqII TatI XbaI XhoI XmaI ZraI