Restriction map for
pF1KB4329
(Linear) (Six-base) MAPSORT of: KIBB4329.seq Check: 8084 from: 1 to: 2811
KIBB4329.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1795 2055 2811
Size: 1795 260 756
Fragments arranged by size:
1795 756 260
AccI GT'mk_AC
Cuts at: 0 344 2789 2811
Size: 344 2445 22
Fragments arranged by size:
2445 344 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 2430 2533 2692 2767 2783 2811
Size: 862 1568 103 159 75 16 28
Fragments arranged by size:
1568 862 159 103 75 28 16
AfeI AGC'GCT
Cuts at: 0 1168 2811
Size: 1168 1643
AhdI GACnn_n'nnGTC
Cuts at: 0 675 2811
Size: 675 2136
AleI CACnn'nnGTG
Cuts at: 0 1435 2748 2811
Size: 1435 1313 63
Fragments arranged by size:
1435 1313 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 1453 2811
Size: 444 1009 1358
Fragments arranged by size:
1358 1009 444
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2696 2728 2811
Size: 2696 32 83
Fragments arranged by size:
2696 83 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1062 2811
Size: 1062 1749
ApaI G_GGCC'C
Cuts at: 0 2377 2811
Size: 2377 434
ApoI r'AATT_y
Cuts at: 0 1760 2811
Size: 1760 1051
AseI AT'TA_AT
Cuts at: 0 611 2811
Size: 611 2200
AvaI C'yCGr_G
Cuts at: 0 1331 2811
Size: 1331 1480
AvrII C'CTAG_G
Cuts at: 0 1965 2811
Size: 1965 846
BamHI G'GATC_C
Cuts at: 0 399 2811
Size: 399 2412
BanI G'GyrC_C
Cuts at: 0 1580 2106 2811
Size: 1580 526 705
Fragments arranged by size:
1580 705 526
BanII G_rGCy'C
Cuts at: 0 2377 2760 2811
Size: 2377 383 51
Fragments arranged by size:
2377 383 51
BbeI G_GCGC'C
Cuts at: 0 1584 2811
Size: 1584 1227
BbsI GAAGACnn'nnnn_
Cuts at: 0 960 2430 2499 2811
Size: 960 1470 69 312
Fragments arranged by size:
1470 960 312 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 2722 2811
Size: 2722 89
BglI GCCn_nnn'nGGC
Cuts at: 0 256 544 1820 2811
Size: 256 288 1276 991
Fragments arranged by size:
1276 991 288 256
Bme1580I G_kGCm'C
Cuts at: 0 2204 2377 2811
Size: 2204 173 434
Fragments arranged by size:
2204 434 173
BmrI ACTGGGnnnn_n'
Cuts at: 0 1909 2371 2811
Size: 1909 462 440
Fragments arranged by size:
1909 462 440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 2392 2811
Size: 1429 963 419
Fragments arranged by size:
1429 963 419
Bpu10I CC'TnA_GC
Cuts at: 0 1055 2811
Size: 1055 1756
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1025 1462 1539 1853 2811
Size: 1025 437 77 314 958
Fragments arranged by size:
1025 958 437 314 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 1553 2811
Size: 1553 1258
BsaAI yAC'GTr
Cuts at: 0 2710 2811
Size: 2710 101
BsaBI GATnn'nnATC
Cuts at: 0 293 398 515 661 2811
Size: 293 105 117 146 2150
Fragments arranged by size:
2150 293 146 117 105
BsaHI Gr'CG_yC
Cuts at: 0 1581 2811
Size: 1581 1230
BsaWI w'CCGG_w
Cuts at: 0 2176 2811
Size: 2176 635
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2016 2046 2811
Size: 2016 30 765
Fragments arranged by size:
2016 765 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1663 1985 2294 2811
Size: 1663 322 309 517
Fragments arranged by size:
1663 517 322 309
BseYI C'CCAG_C
Cuts at: 0 2296 2811
Size: 2296 515
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1459 2811
Size: 913 546 1352
Fragments arranged by size:
1352 913 546
BsiHKAI G_wGCw'C
Cuts at: 0 1798 2760 2811
Size: 1798 962 51
Fragments arranged by size:
1798 962 51
BsmI GAATG_Cn'
Cuts at: 0 643 2811
Size: 643 2168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1645 2811
Size: 1645 1166
Bsp1286I G_dGCh'C
Cuts at: 0 1798 2204 2377 2760 2811
Size: 1798 406 173 383 51
Fragments arranged by size:
1798 406 383 173 51
BspEI T'CCGG_A
Cuts at: 0 2176 2811
Size: 2176 635
BspHI T'CATG_A
Cuts at: 0 1022 2381 2811
Size: 1022 1359 430
Fragments arranged by size:
1359 1022 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1503 1795 2055 2154 2811
Size: 1503 292 260 99 657
Fragments arranged by size:
1503 657 292 260 99
BsrBI CCG'CTC
Cuts at: 0 375 726 2811
Size: 375 351 2085
Fragments arranged by size:
2085 375 351
BsrDI GCAATG_nn'
Cuts at: 0 1049 1180 2811
Size: 1049 131 1631
Fragments arranged by size:
1631 1049 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2029 2655 2811
Size: 2029 626 156
Fragments arranged by size:
2029 626 156
BstBI TT'CG_AA
Cuts at: 0 1110 2811
Size: 1110 1701
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1149 1947 2811
Size: 1149 798 864
Fragments arranged by size:
1149 864 798
BstYI r'GATC_y
Cuts at: 0 288 399 498 1887 1959 2811
Size: 288 111 99 1389 72 852
Fragments arranged by size:
1389 852 288 111 99 72
Bsu36I CC'TnA_GG
Cuts at: 0 812 907 1853 2129 2811
Size: 812 95 946 276 682
Fragments arranged by size:
946 812 682 276 95
BtgI C'CryG_G
Cuts at: 0 125 326 2811
Size: 125 201 2485
Fragments arranged by size:
2485 201 125
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1269 2811
Size: 1269 1542
BtsI GCAGTG_nn'
Cuts at: 0 767 1829 1961 2067 2811
Size: 767 1062 132 106 744
Fragments arranged by size:
1062 767 744 132 106
ClaI AT'CG_AT
Cuts at: 0 2432 2811
Size: 2432 379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 509 544 2811
Size: 509 35 2267
Fragments arranged by size:
2267 509 35
DraI TTT'AAA
Cuts at: 0 1895 2576 2811
Size: 1895 681 235
Fragments arranged by size:
1895 681 235
DraIII CAC_nnn'GTG
Cuts at: 0 1295 2113 2811
Size: 1295 818 698
Fragments arranged by size:
1295 818 698
DrdI GACnn_nn'nnGTC
Cuts at: 0 1982 2811
Size: 1982 829
EaeI y'GGCC_r
Cuts at: 0 248 2309 2699 2811
Size: 248 2061 390 112
Fragments arranged by size:
2061 390 248 112
EcoICRI GAG'CTC
Cuts at: 0 2758 2811
Size: 2758 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 1429 2392 2430 2533 2692 2767 2783
Size: 862 567 963 38 103 159 75 16
Cuts at: 2783 2811
Size: 28
Fragments arranged by size:
963 862 567 159 103 75 38 28
16
EcoRI G'AATT_C
Cuts at: 0 1760 2811
Size: 1760 1051
EcoRV GAT'ATC
Cuts at: 0 450 2811
Size: 450 2361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1894 2811
Size: 1894 917
HaeII r_GCGC'y
Cuts at: 0 1170 1584 2502 2811
Size: 1170 414 918 309
Fragments arranged by size:
1170 918 414 309
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 661 693 2379 2411 2811
Size: 168 32 461 32 1686 32 400
Fragments arranged by size:
1686 461 400 168 32 32 32
HincII GTy'rAC
Cuts at: 0 1101 2811
Size: 1101 1710
HindIII A'AGCT_T
Cuts at: 0 199 1401 2811
Size: 199 1202 1410
Fragments arranged by size:
1410 1202 199
KasI G'GCGC_C
Cuts at: 0 1580 2811
Size: 1580 1231
MfeI C'AATT_G
Cuts at: 0 1229 2811
Size: 1229 1582
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 995 2811
Size: 995 1816
MscI TGG'CCA
Cuts at: 0 250 2311 2811
Size: 250 2061 500
Fragments arranged by size:
2061 500 250
MslI CAynn'nnrTG
Cuts at: 0 1147 1435 1945 2209 2386 2576 2748 2811
Size: 1147 288 510 264 177 190 172 63
Fragments arranged by size:
1147 510 288 264 190 177 172 63
MspA1I CmG'CkG
Cuts at: 0 775 2450 2811
Size: 775 1675 361
Fragments arranged by size:
1675 775 361
NarI GG'CG_CC
Cuts at: 0 1581 2811
Size: 1581 1230
NcoI C'CATG_G
Cuts at: 0 326 2811
Size: 326 2485
NspI r_CATG'y
Cuts at: 0 2658 2811
Size: 2658 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 465 2811
Size: 465 2346
PfoI T'CCnGG_A
Cuts at: 0 665 1406 2811
Size: 665 741 1405
Fragments arranged by size:
1405 741 665
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1294 1326 2172 2204 2811
Size: 1294 32 846 32 607
Fragments arranged by size:
1294 846 607 32 32
PshAI GACnn'nnGTC
Cuts at: 0 1907 2811
Size: 1907 904
PspOMI G'GGCC_C
Cuts at: 0 2373 2811
Size: 2373 438
PspXI vC'TCGA_Gb
Cuts at: 0 1331 2811
Size: 1331 1480
PstI C_TGCA'G
Cuts at: 0 1164 2151 2243 2811
Size: 1164 987 92 568
Fragments arranged by size:
1164 987 568 92
PvuII CAG'CTG
Cuts at: 0 2450 2811
Size: 2450 361
SacI G_AGCT'C
Cuts at: 0 2760 2811
Size: 2760 51
SexAI A'CCwGG_T
Cuts at: 0 2109 2811
Size: 2109 702
SfcI C'TryA_G
Cuts at: 0 1085 1160 2147 2239 2811
Size: 1085 75 987 92 572
Fragments arranged by size:
1085 987 572 92 75
SfoI GGC'GCC
Cuts at: 0 1582 2811
Size: 1582 1229
SmlI C'TyrA_G
Cuts at: 0 1040 1331 1477 1518 1868 2811
Size: 1040 291 146 41 350 943
Fragments arranged by size:
1040 943 350 291 146 41
SnaBI TAC'GTA
Cuts at: 0 2710 2811
Size: 2710 101
SspI AAT'ATT
Cuts at: 0 44 2811
Size: 44 2767
StuI AGG'CCT
Cuts at: 0 939 2811
Size: 939 1872
StyI C'CwwG_G
Cuts at: 0 311 326 1965 2196 2369 2811
Size: 311 15 1639 231 173 442
Fragments arranged by size:
1639 442 311 231 173 15
SwaI ATTT'AAAT
Cuts at: 0 2576 2811
Size: 2576 235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 587 1447 1897 2004 2811
Size: 587 860 450 107 807
Fragments arranged by size:
860 807 587 450 107
TatI w'GTAC_w
Cuts at: 0 980 2811
Size: 980 1831
XbaI T'CTAG_A
Cuts at: 0 798 2811
Size: 798 2013
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 759 1740 2304 2637 2811
Size: 759 981 564 333 174
Fragments arranged by size:
981 759 564 333 174
XhoI C'TCGA_G
Cuts at: 0 1331 2811
Size: 1331 1480
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AleI AlfI AloI
AlwNI ApaI ApoI AseI AvaI AvrII BamHI BanI
BanII BbeI BbsI BciVI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EcoICRI Eco57MI EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NspI PflMI PfoI PpiI PshAI
PspOMI PspXI PstI PvuII SacI SexAI SfcI SfoI
SmlI SnaBI SspI StuI StyI SwaI TaqII TatI
XbaI XcmI XhoI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI ApaLI AscI
AsiSI BaeI BbvCI BcgI BclI BfrBI BglII BlpI
BmgBI BmtI BplI BsiEI BsiWI BsrFI BsrGI BssHII
BssSI BstEII BstZ17I EagI EarI EciI EcoNI EcoO109I
FseI FspI FspAI HpaI KpnI MluI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PpuMI PsiI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SpeI SphI SrfI TstI Tth111I XmaI XmnI
ZraI