Restriction map for
pF1KB4334
(Linear) (Six-base) MAPSORT of: KIBB4334.seq Check: 3226 from: 1 to: 1419
KIBB4334.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1419
Size: 727 692
AflIII A'CryG_T
Cuts at: 0 98 920 1419
Size: 98 822 499
Fragments arranged by size:
822 499 98
AhdI GACnn_n'nnGTC
Cuts at: 0 381 1350 1419
Size: 381 969 69
Fragments arranged by size:
969 381 69
AleI CACnn'nnGTG
Cuts at: 0 844 1419
Size: 844 575
ApoI r'AATT_y
Cuts at: 0 1167 1419
Size: 1167 252
AvaI C'yCGr_G
Cuts at: 0 91 657 832 1223 1419
Size: 91 566 175 391 196
Fragments arranged by size:
566 391 196 175 91
BamHI G'GATC_C
Cuts at: 0 302 1419
Size: 302 1117
BanI G'GyrC_C
Cuts at: 0 351 682 1419
Size: 351 331 737
Fragments arranged by size:
737 351 331
BanII G_rGCy'C
Cuts at: 0 458 623 1419
Size: 458 165 796
Fragments arranged by size:
796 458 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 418 663 1279 1286 1419
Size: 418 245 616 7 133
Fragments arranged by size:
616 418 245 133 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1318 1352 1419
Size: 1318 34 67
Fragments arranged by size:
1318 67 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 724 1419
Size: 724 695
BfrBI ATG'CAT
Cuts at: 0 482 811 1419
Size: 482 329 608
Fragments arranged by size:
608 482 329
Bme1580I G_kGCm'C
Cuts at: 0 356 1419
Size: 356 1063
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 847 1238 1419
Size: 847 391 181
Fragments arranged by size:
847 391 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 985 1419
Size: 985 434
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 877 1146 1419
Size: 660 217 269 273
Fragments arranged by size:
660 273 269 217
BsaBI GATnn'nnATC
Cuts at: 0 196 1419
Size: 196 1223
BsaWI w'CCGG_w
Cuts at: 0 601 1419
Size: 601 818
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 389 419 1419
Size: 389 30 1000
Fragments arranged by size:
1000 389 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1302 1419
Size: 1302 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 1419
Size: 507 912
BsiHKAI G_wGCw'C
Cuts at: 0 623 647 1419
Size: 623 24 772
Fragments arranged by size:
772 623 24
BsmI GAATG_Cn'
Cuts at: 0 1341 1419
Size: 1341 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 109 1419
Size: 109 1310
Bsp1286I G_dGCh'C
Cuts at: 0 356 458 623 647 1419
Size: 356 102 165 24 772
Fragments arranged by size:
772 356 165 102 24
BstAPI GCAn_nnn'nTGC
Cuts at: 0 817 1419
Size: 817 602
BstYI r'GATC_y
Cuts at: 0 302 1419
Size: 302 1117
Bsu36I CC'TnA_GG
Cuts at: 0 493 1419
Size: 493 926
BtgI C'CryG_G
Cuts at: 0 554 1419
Size: 554 865
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 189 1094 1419
Size: 189 905 325
Fragments arranged by size:
905 325 189
EarI CTCTTCn'nnn_
Cuts at: 0 712 993 1419
Size: 712 281 426
Fragments arranged by size:
712 426 281
EcoICRI GAG'CTC
Cuts at: 0 621 1419
Size: 621 798
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 985 1419
Size: 727 258 434
Fragments arranged by size:
727 434 258
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1226 1419
Size: 1226 193
EcoO109I rG'GnC_Cy
Cuts at: 0 6 172 1003 1419
Size: 6 166 831 416
Fragments arranged by size:
831 416 166 6
EcoRI G'AATT_C
Cuts at: 0 1167 1419
Size: 1167 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 1336 1368 1419
Size: 367 32 937 32 51
Fragments arranged by size:
937 367 51 32 32
HincII GTy'rAC
Cuts at: 0 234 981 1106 1416 1419
Size: 234 747 125 310 3
Fragments arranged by size:
747 310 234 125 3
HindIII A'AGCT_T
Cuts at: 0 1021 1419
Size: 1021 398
HpaI GTT'AAC
Cuts at: 0 1416 1419
Size: 1416 3
MslI CAynn'nnrTG
Cuts at: 0 217 434 844 919 1419
Size: 217 217 410 75 500
Fragments arranged by size:
500 410 217 217 75
NsiI A_TGCA'T
Cuts at: 0 484 813 1419
Size: 484 329 606
Fragments arranged by size:
606 484 329
NspI r_CATG'y
Cuts at: 0 102 482 924 1221 1419
Size: 102 380 442 297 198
Fragments arranged by size:
442 380 297 198 102
PciI A'CATG_T
Cuts at: 0 98 920 1419
Size: 98 822 499
Fragments arranged by size:
822 499 98
PfoI T'CCnGG_A
Cuts at: 0 1354 1419
Size: 1354 65
PpuMI rG'GwC_Cy
Cuts at: 0 172 1419
Size: 172 1247
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 715 747 1419
Size: 715 32 672
Fragments arranged by size:
715 672 32
SacI G_AGCT'C
Cuts at: 0 623 1419
Size: 623 796
SanDI GG'GwC_CC
Cuts at: 0 172 1419
Size: 172 1247
SmaI CCC'GGG
Cuts at: 0 93 1419
Size: 93 1326
SmlI C'TyrA_G
Cuts at: 0 657 1223 1419
Size: 657 566 196
Fragments arranged by size:
657 566 196
SphI G_CATG'C
Cuts at: 0 482 1221 1419
Size: 482 739 198
Fragments arranged by size:
739 482 198
SspI AAT'ATT
Cuts at: 0 271 1098 1419
Size: 271 827 321
Fragments arranged by size:
827 321 271
StyI C'CwwG_G
Cuts at: 0 1040 1419
Size: 1040 379
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 774 886 1419
Size: 339 435 112 533
Fragments arranged by size:
533 435 339 112
TatI w'GTAC_w
Cuts at: 0 1202 1406 1419
Size: 1202 204 13
Fragments arranged by size:
1202 204 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 389 421 1419
Size: 389 32 998
Fragments arranged by size:
998 389 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 216 1419
Size: 216 1203
XhoI C'TCGA_G
Cuts at: 0 657 1223 1419
Size: 657 566 196
Fragments arranged by size:
657 566 196
XmaI C'CCGG_G
Cuts at: 0 91 1419
Size: 91 1328
Enzymes that do cut:
AcuI AflIII AhdI AleI ApoI AvaI BamHI BanI
BanII BbsI BcgI BciVI BfrBI Bme1580I BplI BpmI
BsaI BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BstAPI BstYI Bsu36I BtgI BtgZI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII HindIII
HpaI MslI NsiI NspI PciI PfoI PpuMI PsrI
SacI SanDI SmaI SmlI SphI SspI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BclI BglI BglII BlpI
BmgBI BmrI BmtI Bpu10I BpuEI BsaAI BsaHI BseYI
BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoRV FalI FseI FspI FspAI HaeII KasI KpnI
MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI StuI SwaI Tth111I XbaI XmnI ZraI