(Linear) (Six-base) MAPSORT of: KIBB4343.seq  Check: 132  from: 1  to: 1092



KIBB4343.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AccI GT'mk_AC

Cuts at:      0     599    1092 
   Size:        599     493
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     440     707    1092 
   Size:        440     267     385

  Fragments arranged by size: 

                 440     385     267
 
AleI CACnn'nnGTG

Cuts at:      0     744    1092 
   Size:        744     348
 
AlwNI CAG_nnn'CTG

Cuts at:      0      24    1092 
   Size:         24    1068
 
ApaI G_GGCC'C

Cuts at:      0       9    1092 
   Size:          9    1083
 
ApaLI G'TGCA_C

Cuts at:      0     659     999    1092 
   Size:        659     340      93

  Fragments arranged by size: 

                 659     340      93
 
ApoI r'AATT_y

Cuts at:      0     605    1055    1092 
   Size:        605     450      37

  Fragments arranged by size: 

                 605     450      37
 
BanII G_rGCy'C

Cuts at:      0       9    1040    1092 
   Size:          9    1031      52

  Fragments arranged by size: 

                1031      52       9
 
BbvCI CC'TCA_GC

Cuts at:      0     289    1092 
   Size:        289     803
 
Bme1580I G_kGCm'C

Cuts at:      0       9     663    1003    1092 
   Size:          9     654     340      89

  Fragments arranged by size: 

                 654     340      89       9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      34    1092 
   Size:         34    1058
 
Bpu10I CC'TnA_GC

Cuts at:      0     289    1092 
   Size:        289     803
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181    1092 
   Size:        181     911
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     219     510    1092 
   Size:        219     291     582

  Fragments arranged by size: 

                 582     291     219
 
BsaBI GATnn'nnATC

Cuts at:      0     404    1092 
   Size:        404     688
 
BsaHI Gr'CG_yC

Cuts at:      0     449    1092 
   Size:        449     643
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     976    1006    1092 
   Size:        976      30      86

  Fragments arranged by size: 

                 976      86      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     768     936    1092 
   Size:        768     168     156

  Fragments arranged by size: 

                 768     168     156
 
BsiHKAI G_wGCw'C

Cuts at:      0     663    1003    1040    1092 
   Size:        663     340      37      52

  Fragments arranged by size: 

                 663     340      52      37
 
BsmI GAATG_Cn'

Cuts at:      0     585    1092 
   Size:        585     507
 
Bsp1286I G_dGCh'C

Cuts at:      0       9     663    1003    1040    1092 
   Size:          9     654     340      37      52

  Fragments arranged by size: 

                 654     340      52      37       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     256    1092 
   Size:        256     836
 
BtgI C'CryG_G

Cuts at:      0      10    1092 
   Size:         10    1082
 
DraIII CAC_nnn'GTG

Cuts at:      0    1007    1092 
   Size:       1007      85
 
EarI CTCTTCn'nnn_

Cuts at:      0      71     142    1092 
   Size:         71      71     950

  Fragments arranged by size: 

                 950      71      71
 
EcoICRI GAG'CTC

Cuts at:      0    1038    1092 
   Size:       1038      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     440     707    1092 
   Size:        440     267     385

  Fragments arranged by size: 

                 440     385     267
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     320    1092 
   Size:        320     772
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6    1092 
   Size:          6    1086
 
EcoRI G'AATT_C

Cuts at:      0    1055    1092 
   Size:       1055      37
 
EcoRV GAT'ATC

Cuts at:      0      91    1092 
   Size:         91    1001
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     503    1092 
   Size:        503     589
 
MslI CAynn'nnrTG

Cuts at:      0     744    1092 
   Size:        744     348
 
MspA1I CmG'CkG

Cuts at:      0      12    1092 
   Size:         12    1080
 
PasI CC'CwG_GG

Cuts at:      0      71     628    1092 
   Size:         71     557     464

  Fragments arranged by size: 

                 557     464      71
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     634    1092 
   Size:        634     458
 
PspOMI G'GGCC_C

Cuts at:      0       5    1092 
   Size:          5    1087
 
SacI G_AGCT'C

Cuts at:      0    1040    1092 
   Size:       1040      52
 
SacII CC_GC'GG

Cuts at:      0      13    1092 
   Size:         13    1079
 
SmlI C'TyrA_G

Cuts at:      0     160    1092 
   Size:        160     932
 
SspI AAT'ATT

Cuts at:      0     551    1092 
   Size:        551     541
 
StyI C'CwwG_G

Cuts at:      0      53    1092 
   Size:         53    1039
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     365    1092 
   Size:        365     727
 
TatI w'GTAC_w

Cuts at:      0     469     886    1092 
   Size:        469     417     206

  Fragments arranged by size: 

                 469     417     206
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     530    1092 
   Size:        530     562
 

 Enzymes that do cut:

     AccI     AcuI     AleI    AlwNI     ApaI    ApaLI     ApoI    BanII
    BbvCI Bme1580I     BmrI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI
    BsaXI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI     BtgI   DraIII
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     MslI   MspA1I     PasI    PflMI   PspOMI     SacI    SacII     SmlI
     SspI     StyI    TaqII     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI     BpmI
    BsaAI    BsaWI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EciI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI