Restriction map for
pF1KB4351
(Linear) (Six-base) MAPSORT of: KIBB4351.seq Check: 24 from: 1 to: 1080
KIBB4351.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 39 1080
Size: 39 1041
AclI AA'CG_TT
Cuts at: 0 431 1080
Size: 431 649
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 1080
Size: 216 864
AflIII A'CryG_T
Cuts at: 0 568 1080
Size: 568 512
AlwNI CAG_nnn'CTG
Cuts at: 0 921 1080
Size: 921 159
ApaI G_GGCC'C
Cuts at: 0 31 1080
Size: 31 1049
ApoI r'AATT_y
Cuts at: 0 600 768 1080
Size: 600 168 312
Fragments arranged by size:
600 312 168
AvaI C'yCGr_G
Cuts at: 0 954 1080
Size: 954 126
BamHI G'GATC_C
Cuts at: 0 733 1080
Size: 733 347
BanI G'GyrC_C
Cuts at: 0 100 816 1080
Size: 100 716 264
Fragments arranged by size:
716 264 100
BanII G_rGCy'C
Cuts at: 0 31 585 968 1080
Size: 31 554 383 112
Fragments arranged by size:
554 383 112 31
BbeI G_GCGC'C
Cuts at: 0 104 1080
Size: 104 976
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 755 1080
Size: 355 400 325
Fragments arranged by size:
400 355 325
BbvCI CC'TCA_GC
Cuts at: 0 361 1080
Size: 361 719
BciVI GTATCCnnnnn_n'
Cuts at: 0 1063 1080
Size: 1063 17
BclI T'GATC_A
Cuts at: 0 534 1080
Size: 534 546
BglII A'GATC_T
Cuts at: 0 330 491 1075 1080
Size: 330 161 584 5
Fragments arranged by size:
584 330 161 5
Bme1580I G_kGCm'C
Cuts at: 0 31 1080
Size: 31 1049
BmgBI CAC'GTC
Cuts at: 0 60 1080
Size: 60 1020
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 958 1080
Size: 569 389 122
Fragments arranged by size:
569 389 122
Bpu10I CC'TnA_GC
Cuts at: 0 361 1080
Size: 361 719
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 472 1080
Size: 433 39 608
Fragments arranged by size:
608 433 39
BsaBI GATnn'nnATC
Cuts at: 0 217 738 1074 1080
Size: 217 521 336 6
Fragments arranged by size:
521 336 217 6
BsaHI Gr'CG_yC
Cuts at: 0 101 1080
Size: 101 979
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 421 1080
Size: 421 659
BsiHKAI G_wGCw'C
Cuts at: 0 121 182 276 1080
Size: 121 61 94 804
Fragments arranged by size:
804 121 94 61
BsmI GAATG_Cn'
Cuts at: 0 647 1080
Size: 647 433
Bsp1286I G_dGCh'C
Cuts at: 0 31 121 182 276 585 968 1080
Size: 31 90 61 94 309 383 112
Fragments arranged by size:
383 309 112 94 90 61 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 39 470 1080
Size: 39 431 610
Fragments arranged by size:
610 431 39
BsrDI GCAATG_nn'
Cuts at: 0 372 1080
Size: 372 708
BstAPI GCAn_nnn'nTGC
Cuts at: 0 116 1080
Size: 116 964
BstXI CCAn_nnnn'nTGG
Cuts at: 0 858 1080
Size: 858 222
BstYI r'GATC_y
Cuts at: 0 330 395 491 522 733 1075 1080
Size: 330 65 96 31 211 342 5
Fragments arranged by size:
342 330 211 96 65 31 5
Bsu36I CC'TnA_GG
Cuts at: 0 205 1016 1080
Size: 205 811 64
Fragments arranged by size:
811 205 64
BtgI C'CryG_G
Cuts at: 0 42 820 1080
Size: 42 778 260
Fragments arranged by size:
778 260 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 954 1080
Size: 954 126
DraI TTT'AAA
Cuts at: 0 773 1080
Size: 773 307
EaeI y'GGCC_r
Cuts at: 0 563 1080
Size: 563 517
EarI CTCTTCn'nnn_
Cuts at: 0 803 1040 1080
Size: 803 237 40
Fragments arranged by size:
803 237 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 569 958 1080
Size: 216 353 389 122
Fragments arranged by size:
389 353 216 122
EcoO109I rG'GnC_Cy
Cuts at: 0 315 1080
Size: 315 765
EcoRI G'AATT_C
Cuts at: 0 600 1080
Size: 600 480
HaeII r_GCGC'y
Cuts at: 0 104 1080
Size: 104 976
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 140 172 619 651 1080
Size: 140 32 447 32 429
Fragments arranged by size:
447 429 140 32 32
HincII GTy'rAC
Cuts at: 0 138 881 1080
Size: 138 743 199
Fragments arranged by size:
743 199 138
KasI G'GCGC_C
Cuts at: 0 100 1080
Size: 100 980
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1080
Size: 482 598
MscI TGG'CCA
Cuts at: 0 565 1080
Size: 565 515
MspA1I CmG'CkG
Cuts at: 0 44 708 1080
Size: 44 664 372
Fragments arranged by size:
664 372 44
NarI GG'CG_CC
Cuts at: 0 101 1080
Size: 101 979
NcoI C'CATG_G
Cuts at: 0 820 1080
Size: 820 260
PflMI CCAn_nnn'nTGG
Cuts at: 0 572 720 1080
Size: 572 148 360
Fragments arranged by size:
572 360 148
PpuMI rG'GwC_Cy
Cuts at: 0 315 1080
Size: 315 765
PspOMI G'GGCC_C
Cuts at: 0 27 1080
Size: 27 1053
PvuII CAG'CTG
Cuts at: 0 708 1080
Size: 708 372
SacII CC_GC'GG
Cuts at: 0 45 1080
Size: 45 1035
SfcI C'TryA_G
Cuts at: 0 638 1080
Size: 638 442
SfoI GGC'GCC
Cuts at: 0 102 1080
Size: 102 978
SmlI C'TyrA_G
Cuts at: 0 448 487 1080
Size: 448 39 593
Fragments arranged by size:
593 448 39
StyI C'CwwG_G
Cuts at: 0 605 820 1080
Size: 605 215 260
Fragments arranged by size:
605 260 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 944 1080
Size: 944 136
TatI w'GTAC_w
Cuts at: 0 309 1080
Size: 309 771
Enzymes that do cut:
AarI AclI AcuI AflIII AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaBI BsaHI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI DraI EaeI EarI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI MscI
MspA1I NarI NcoI PflMI PpuMI PspOMI PvuII SacII
SfcI SfoI SmlI StyI TaqII TatI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BfrBI BglI BlpI BmrI BmtI BplI BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MslI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI