Restriction map for
pF1KB4360
(Linear) (Six-base) MAPSORT of: KIBB4360.seq Check: 692 from: 1 to: 2385
KIBB4360.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1496 2385
Size: 1496 889
AatII G_ACGT'C
Cuts at: 0 1328 2385
Size: 1328 1057
AccI GT'mk_AC
Cuts at: 0 1045 2060 2385
Size: 1045 1015 325
Fragments arranged by size:
1045 1015 325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2317 2385
Size: 2317 68
AfeI AGC'GCT
Cuts at: 0 596 2385
Size: 596 1789
AleI CACnn'nnGTG
Cuts at: 0 1204 2385
Size: 1204 1181
AlwNI CAG_nnn'CTG
Cuts at: 0 448 2385
Size: 448 1937
ApaI G_GGCC'C
Cuts at: 0 2188 2385
Size: 2188 197
ApoI r'AATT_y
Cuts at: 0 522 721 896 2385
Size: 522 199 175 1489
Fragments arranged by size:
1489 522 199 175
AvaI C'yCGr_G
Cuts at: 0 182 754 797 1727 1782 1831 2179 2385
Size: 182 572 43 930 55 49 348 206
Fragments arranged by size:
930 572 348 206 182 55 49 43
BanI G'GyrC_C
Cuts at: 0 750 1438 1704 1722 2175 2218 2385
Size: 750 688 266 18 453 43 167
Fragments arranged by size:
750 688 453 266 167 43 18
BanII G_rGCy'C
Cuts at: 0 221 623 657 1832 2188 2385
Size: 221 402 34 1175 356 197
Fragments arranged by size:
1175 402 356 221 197 34
BbeI G_GCGC'C
Cuts at: 0 1708 2385
Size: 1708 677
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2063 2097 2385
Size: 2063 34 288
Fragments arranged by size:
2063 288 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1694 2385
Size: 1694 691
BfrBI ATG'CAT
Cuts at: 0 198 2385
Size: 198 2187
BglI GCCn_nnn'nGGC
Cuts at: 0 142 1570 2385
Size: 142 1428 815
Fragments arranged by size:
1428 815 142
BlpI GC'TnA_GC
Cuts at: 0 622 1618 1885 2385
Size: 622 996 267 500
Fragments arranged by size:
996 622 500 267
Bme1580I G_kGCm'C
Cuts at: 0 755 1727 2180 2188 2385
Size: 755 972 453 8 197
Fragments arranged by size:
972 755 453 197 8
BmgBI CAC'GTC
Cuts at: 0 2211 2385
Size: 2211 174
BmtI G_CTAG'C
Cuts at: 0 16 2385
Size: 16 2369
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 2385
Size: 96 2289
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 2385
Size: 1038 1347
Bpu10I CC'TnA_GC
Cuts at: 0 617 2385
Size: 617 1768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1801 2385
Size: 1801 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 1323 1512 2385
Size: 1323 189 873
Fragments arranged by size:
1323 873 189
BsaAI yAC'GTr
Cuts at: 0 1974 2385
Size: 1974 411
BsaHI Gr'CG_yC
Cuts at: 0 1325 1655 1705 2385
Size: 1325 330 50 680
Fragments arranged by size:
1325 680 330 50
BsaWI w'CCGG_w
Cuts at: 0 2263 2363 2385
Size: 2263 100 22
Fragments arranged by size:
2263 100 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 268 298 1591 1621 1978 2008 2385
Size: 268 30 1293 30 357 30 377
Fragments arranged by size:
1293 377 357 268 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 2157 2385
Size: 622 1535 228
Fragments arranged by size:
1535 622 228
BseYI C'CCAG_C
Cuts at: 0 437 1997 2385
Size: 437 1560 388
Fragments arranged by size:
1560 437 388
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 623 1384 1954 2157 2385
Size: 361 262 761 570 203 228
Fragments arranged by size:
761 570 361 262 228 203
BsiEI CG_ry'CG
Cuts at: 0 1736 2162 2166 2385
Size: 1736 426 4 219
Fragments arranged by size:
1736 426 219 4
BsiHKAI G_wGCw'C
Cuts at: 0 221 436 623 1301 1979 2114 2385
Size: 221 215 187 678 678 135 271
Fragments arranged by size:
678 678 271 221 215 187 135
BsmI GAATG_Cn'
Cuts at: 0 200 2385
Size: 200 2185
Bsp1286I G_dGCh'C
Cuts at: 0 221 436 623 657 755 1301 1727 1832
Size: 221 215 187 34 98 546 426 105
Cuts at: 1832 1979 2114 2180 2188 2385
Size: 147 135 66 8 197
Fragments arranged by size:
546 426 221 215 197 187 147 135
105 98 66 34 8
BspEI T'CCGG_A
Cuts at: 0 2363 2385
Size: 2363 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1496 2015 2385
Size: 1496 519 370
Fragments arranged by size:
1496 519 370
BsrBI CCG'CTC
Cuts at: 0 612 796 2216 2385
Size: 612 184 1420 169
Fragments arranged by size:
1420 612 184 169
BsrFI r'CCGG_y
Cuts at: 0 241 2278 2385
Size: 241 2037 107
Fragments arranged by size:
2037 241 107
BssHII G'CGCG_C
Cuts at: 0 188 2385
Size: 188 2197
BstEII G'GTnAC_C
Cuts at: 0 1452 1752 2385
Size: 1452 300 633
Fragments arranged by size:
1452 633 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1764 2332 2385
Size: 1764 568 53
Fragments arranged by size:
1764 568 53
Bsu36I CC'TnA_GG
Cuts at: 0 1543 2385
Size: 1543 842
BtgI C'CryG_G
Cuts at: 0 1602 1823 2385
Size: 1602 221 562
Fragments arranged by size:
1602 562 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 866 2385
Size: 866 1519
BtsI GCAGTG_nn'
Cuts at: 0 635 1416 2132 2385
Size: 635 781 716 253
Fragments arranged by size:
781 716 635 253
EaeI y'GGCC_r
Cuts at: 0 134 176 1527 1733 1901 1955 2385
Size: 134 42 1351 206 168 54 430
Fragments arranged by size:
1351 430 206 168 134 54 42
EagI C'GGCC_G
Cuts at: 0 1733 2385
Size: 1733 652
EarI CTCTTCn'nnn_
Cuts at: 0 2101 2385
Size: 2101 284
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 647 2385
Size: 647 1738
EcoICRI GAG'CTC
Cuts at: 0 219 621 2385
Size: 219 402 1764
Fragments arranged by size:
1764 402 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 2317 2385
Size: 1038 1279 68
Fragments arranged by size:
1279 1038 68
EcoO109I rG'GnC_Cy
Cuts at: 0 972 1563 2184 2185 2225 2385
Size: 972 591 621 1 40 160
Fragments arranged by size:
972 621 591 160 40 1
FspI TGC'GCA
Cuts at: 0 1870 2385
Size: 1870 515
FspAI rTGC'GCAy
Cuts at: 0 1870 2385
Size: 1870 515
HaeII r_GCGC'y
Cuts at: 0 598 1421 1618 1633 1708 2333 2385
Size: 598 823 197 15 75 625 52
Fragments arranged by size:
823 625 598 197 75 52 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1417 1449 1591 1623 1765 1797
Size: 1102 32 283 32 142 32 142 32
Cuts at: 1797 1978 2010 2385
Size: 181 32 375
Fragments arranged by size:
1102 375 283 181 142 142 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 1704 2385
Size: 1704 681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1346 2385
Size: 1346 1039
MscI TGG'CCA
Cuts at: 0 136 178 2385
Size: 136 42 2207
Fragments arranged by size:
2207 136 42
MslI CAynn'nnrTG
Cuts at: 0 533 1204 1270 2385
Size: 533 671 66 1115
Fragments arranged by size:
1115 671 533 66
MspA1I CmG'CkG
Cuts at: 0 1604 1689 1812 2013 2385
Size: 1604 85 123 201 372
Fragments arranged by size:
1604 372 201 123 85
NaeI GCC'GGC
Cuts at: 0 2280 2385
Size: 2280 105
NarI GG'CG_CC
Cuts at: 0 1705 2385
Size: 1705 680
NdeI CA'TA_TG
Cuts at: 0 819 2385
Size: 819 1566
NgoMIV G'CCGG_C
Cuts at: 0 2278 2385
Size: 2278 107
NheI G'CTAG_C
Cuts at: 0 12 2385
Size: 12 2373
NsiI A_TGCA'T
Cuts at: 0 200 2385
Size: 200 2185
NspI r_CATG'y
Cuts at: 0 1848 2385
Size: 1848 537
PasI CC'CwG_GG
Cuts at: 0 2325 2385
Size: 2325 60
PfoI T'CCnGG_A
Cuts at: 0 667 2385
Size: 667 1718
PmlI CAC'GTG
Cuts at: 0 1974 2385
Size: 1974 411
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2275 2307 2385
Size: 2275 32 78
Fragments arranged by size:
2275 78 32
PpuMI rG'GwC_Cy
Cuts at: 0 2225 2385
Size: 2225 160
PshAI GACnn'nnGTC
Cuts at: 0 155 2385
Size: 155 2230
PsiI TTA'TAA
Cuts at: 0 941 2385
Size: 941 1444
PspOMI G'GGCC_C
Cuts at: 0 2184 2385
Size: 2184 201
PstI C_TGCA'G
Cuts at: 0 365 1571 1694 2385
Size: 365 1206 123 691
Fragments arranged by size:
1206 691 365 123
PvuII CAG'CTG
Cuts at: 0 1812 2385
Size: 1812 573
SacI G_AGCT'C
Cuts at: 0 221 623 2385
Size: 221 402 1762
Fragments arranged by size:
1762 402 221
SacII CC_GC'GG
Cuts at: 0 1605 2385
Size: 1605 780
SanDI GG'GwC_CC
Cuts at: 0 2225 2385
Size: 2225 160
SapI GCTCTTCn'nnn_
Cuts at: 0 2101 2385
Size: 2101 284
SbfI CC_TGCA'GG
Cuts at: 0 1571 2385
Size: 1571 814
SexAI A'CCwGG_T
Cuts at: 0 1748 2063 2385
Size: 1748 315 322
Fragments arranged by size:
1748 322 315
SfcI C'TryA_G
Cuts at: 0 361 998 1085 1567 1690 2385
Size: 361 637 87 482 123 695
Fragments arranged by size:
695 637 482 361 123 87
SfoI GGC'GCC
Cuts at: 0 1706 2385
Size: 1706 679
SmaI CCC'GGG
Cuts at: 0 1729 1833 2385
Size: 1729 104 552
Fragments arranged by size:
1729 552 104
SmlI C'TyrA_G
Cuts at: 0 797 1816 2385
Size: 797 1019 569
Fragments arranged by size:
1019 797 569
SrfI GCCC'GGGC
Cuts at: 0 1833 2385
Size: 1833 552
StyI C'CwwG_G
Cuts at: 0 846 1757 2385
Size: 846 911 628
Fragments arranged by size:
911 846 628
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 451 1296 1366 2152 2385
Size: 451 845 70 786 233
Fragments arranged by size:
845 786 451 233 70
TatI w'GTAC_w
Cuts at: 0 1244 2385
Size: 1244 1141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 268 300 2385
Size: 268 32 2085
Fragments arranged by size:
2085 268 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1911 2385
Size: 1911 474
XhoI C'TCGA_G
Cuts at: 0 797 2385
Size: 797 1588
XmaI C'CCGG_G
Cuts at: 0 1727 1831 2385
Size: 1727 104 554
Fragments arranged by size:
1727 554 104
ZraI GAC'GTC
Cuts at: 0 1326 2385
Size: 1326 1059
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BcgI BciVI BfrBI
BglI BlpI Bme1580I BmgBI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BstEII BstXI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NsiI NspI PasI
PfoI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuII SacI SacII SanDI SapI SbfI SexAI SfcI
SfoI SmaI SmlI SrfI StyI TaqII TatI TstI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AlfI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BclI BglII BmrI BsaBI BsiWI BsmBI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstYI BstZ17I ClaI
CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI HincII HindIII HpaI KpnI MfeI MluI NcoI
NotI NruI PacI PciI PflMI PmeI PspXI PsrI
PvuI RsrII SalI ScaI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI Tth111I XbaI XmnI