(Linear) (Six-base) MAPSORT of: KIBB4371.seq  Check: 4642  from: 1  to: 474



KIBB4371.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

Acc65I G'GTAC_C

Cuts at:      0      75     474 
   Size:         75     399
 
AfeI AGC'GCT

Cuts at:      0     307     474 
   Size:        307     167
 
AflIII A'CryG_T

Cuts at:      0     465     474 
   Size:        465       9
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      59      92     474 
   Size:         59      33     382

  Fragments arranged by size: 

                 382      59      33
 
BanI G'GyrC_C

Cuts at:      0      75     474 
   Size:         75     399
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     116     474 
   Size:        116     358
 
Bme1580I G_kGCm'C

Cuts at:      0     371     474 
   Size:        371     103
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130     474 
   Size:        130     344
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     350     443     474 
   Size:        319      31      93      31

  Fragments arranged by size: 

                 319      93      31      31
 
BsaBI GATnn'nnATC

Cuts at:      0     211     474 
   Size:        211     263
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     316     474 
   Size:        316     158
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     474 
   Size:        241     233
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     474 
   Size:        203     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     371     474 
   Size:        203     168     103

  Fragments arranged by size: 

                 203     168     103
 
Bsu36I CC'TnA_GG

Cuts at:      0     410     474 
   Size:        410      64
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     474 
   Size:         40     434
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     350     443     474 
   Size:        319      31      93      31

  Fragments arranged by size: 

                 319      93      31      31
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     298     474 
   Size:        298     176
 
EcoO109I rG'GnC_Cy

Cuts at:      0     325     406     474 
   Size:        325      81      68

  Fragments arranged by size: 

                 325      81      68
 
HaeII r_GCGC'y

Cuts at:      0     309     474 
   Size:        309     165
 
KpnI G_GTAC'C

Cuts at:      0      79     474 
   Size:         79     395
 
MslI CAynn'nnrTG

Cuts at:      0     217     474 
   Size:        217     257
 
NspI r_CATG'y

Cuts at:      0     469     474 
   Size:        469       5
 
PciI A'CATG_T

Cuts at:      0     465     474 
   Size:        465       9
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     120     195     474 
   Size:        120      75     279

  Fragments arranged by size: 

                 279     120      75
 
PfoI T'CCnGG_A

Cuts at:      0     459     474 
   Size:        459      15
 
PpuMI rG'GwC_Cy

Cuts at:      0     406     474 
   Size:        406      68
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40     474 
   Size:         40     434
 
SfcI C'TryA_G

Cuts at:      0     237     474 
   Size:        237     237
 
StuI AGG'CCT

Cuts at:      0     375     474 
   Size:        375      99
 
StyI C'CwwG_G

Cuts at:      0      71     194     474 
   Size:         71     123     280

  Fragments arranged by size: 

                 280     123      71
 

 Enzymes that do cut:

   Acc65I     AfeI   AflIII     BaeI     BanI     BbsI Bme1580I     BmrI
     BpmI    BsaBI    BseRI     BsgI  BsiHKAI Bsp1286I   Bsu36I     EarI
  Eco57MI    EcoNI EcoO109I    HaeII     KpnI     MslI     NspI     PciI
    PflMI     PfoI    PpuMI     SapI     SfcI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI