Restriction map for
pF1KB4387
(Linear) (Six-base) MAPSORT of: KIBB4387.seq Check: 9967 from: 1 to: 2574
KIBB4387.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 208 484 1096 2574
Size: 208 276 612 1478
Fragments arranged by size:
1478 612 276 208
AclI AA'CG_TT
Cuts at: 0 1528 2574
Size: 1528 1046
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 2574
Size: 201 2373
AfeI AGC'GCT
Cuts at: 0 147 547 2574
Size: 147 400 2027
Fragments arranged by size:
2027 400 147
AflII C'TTAA_G
Cuts at: 0 994 2443 2574
Size: 994 1449 131
Fragments arranged by size:
1449 994 131
AflIII A'CryG_T
Cuts at: 0 563 2574
Size: 563 2011
AleI CACnn'nnGTG
Cuts at: 0 1303 2281 2574
Size: 1303 978 293
Fragments arranged by size:
1303 978 293
AlwNI CAG_nnn'CTG
Cuts at: 0 2037 2143 2574
Size: 2037 106 431
Fragments arranged by size:
2037 431 106
ApaI G_GGCC'C
Cuts at: 0 8 2574
Size: 8 2566
ApoI r'AATT_y
Cuts at: 0 718 2574
Size: 718 1856
BamHI G'GATC_C
Cuts at: 0 1077 1448 1572 2574
Size: 1077 371 124 1002
Fragments arranged by size:
1077 1002 371 124
BanII G_rGCy'C
Cuts at: 0 8 1189 1377 2276 2367 2574
Size: 8 1181 188 899 91 207
Fragments arranged by size:
1181 899 207 188 91 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1792 2391 2409 2494 2574
Size: 1792 599 18 85 80
Fragments arranged by size:
1792 599 85 80 18
BbvCI CC'TCA_GC
Cuts at: 0 2144 2574
Size: 2144 430
BciVI GTATCCnnnnn_n'
Cuts at: 0 1497 2574
Size: 1497 1077
BfrBI ATG'CAT
Cuts at: 0 116 866 2574
Size: 116 750 1708
Fragments arranged by size:
1708 750 116
BglI GCCn_nnn'nGGC
Cuts at: 0 161 617 1025 1177 2099 2574
Size: 161 456 408 152 922 475
Fragments arranged by size:
922 475 456 408 161 152
BglII A'GATC_T
Cuts at: 0 1704 2574
Size: 1704 870
Bme1580I G_kGCm'C
Cuts at: 0 8 1021 1489 2574
Size: 8 1013 468 1085
Fragments arranged by size:
1085 1013 468 8
BmgBI CAC'GTC
Cuts at: 0 185 323 981 2568 2574
Size: 185 138 658 1587 6
Fragments arranged by size:
1587 658 185 138 6
BmrI ACTGGGnnnn_n'
Cuts at: 0 1614 1737 2345 2574
Size: 1614 123 608 229
Fragments arranged by size:
1614 608 229 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1563 2574
Size: 1563 1011
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 618 1132 2574
Size: 279 339 514 1442
Fragments arranged by size:
1442 514 339 279
Bpu10I CC'TnA_GC
Cuts at: 0 2014 2144 2574
Size: 2014 130 430
Fragments arranged by size:
2014 430 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1740 2574
Size: 1740 834
BsaAI yAC'GTr
Cuts at: 0 564 2574
Size: 564 2010
BsaHI Gr'CG_yC
Cuts at: 0 1382 2574
Size: 1382 1192
BsaWI w'CCGG_w
Cuts at: 0 1244 1781 2574
Size: 1244 537 793
Fragments arranged by size:
1244 793 537
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 406 436 1810 1840 2574
Size: 406 30 1374 30 734
Fragments arranged by size:
1374 734 406 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 1699 2000 2139 2383 2574
Size: 34 1665 301 139 244 191
Fragments arranged by size:
1665 301 244 191 139 34
BseYI C'CCAG_C
Cuts at: 0 485 1043 1361 1976 2574
Size: 485 558 318 615 598
Fragments arranged by size:
615 598 558 485 318
BsiHKAI G_wGCw'C
Cuts at: 0 328 437 730 2393 2574
Size: 328 109 293 1663 181
Fragments arranged by size:
1663 328 293 181 109
BsmI GAATG_Cn'
Cuts at: 0 245 868 955 2574
Size: 245 623 87 1619
Fragments arranged by size:
1619 623 245 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 175 1389 2574
Size: 175 1214 1185
Fragments arranged by size:
1214 1185 175
Bsp1286I G_dGCh'C
Cuts at: 0 8 328 437 730 1021 1189 1377 1489
Size: 8 320 109 293 291 168 188 112
Cuts at: 1489 2276 2367 2393 2574
Size: 787 91 26 181
Fragments arranged by size:
787 320 293 291 188 181 168 112
109 91 26 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 208 484 1096 2092 2574
Size: 208 276 612 996 482
Fragments arranged by size:
996 612 482 276 208
BsrDI GCAATG_nn'
Cuts at: 0 993 2025 2574
Size: 993 1032 549
Fragments arranged by size:
1032 993 549
BsrFI r'CCGG_y
Cuts at: 0 755 1214 2574
Size: 755 459 1360
Fragments arranged by size:
1360 755 459
BssSI C'ACGA_G
Cuts at: 0 1219 2574
Size: 1219 1355
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1569 2574
Size: 1569 1005
BstYI r'GATC_y
Cuts at: 0 1077 1448 1572 1704 2574
Size: 1077 371 124 132 870
Fragments arranged by size:
1077 870 371 132 124
Bsu36I CC'TnA_GG
Cuts at: 0 1276 2574
Size: 1276 1298
BtgI C'CryG_G
Cuts at: 0 50 747 1377 2279 2574
Size: 50 697 630 902 295
Fragments arranged by size:
902 697 630 295 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 152 281 809 1176 2574
Size: 152 129 528 367 1398
Fragments arranged by size:
1398 528 367 152 129
BtsI GCAGTG_nn'
Cuts at: 0 71 606 766 2159 2574
Size: 71 535 160 1393 415
Fragments arranged by size:
1393 535 415 160 71
ClaI AT'CG_AT
Cuts at: 0 1064 1763 2574
Size: 1064 699 811
Fragments arranged by size:
1064 811 699
DrdI GACnn_nn'nnGTC
Cuts at: 0 189 2476 2574
Size: 189 2287 98
Fragments arranged by size:
2287 189 98
EaeI y'GGCC_r
Cuts at: 0 270 538 698 1307 1716 1832 2111 2156
Size: 270 268 160 609 409 116 279 45
Cuts at: 2156 2574
Size: 418
Fragments arranged by size:
609 418 409 279 270 268 160 116
45
EarI CTCTTCn'nnn_
Cuts at: 0 1207 1392 1513 1966 2574
Size: 1207 185 121 453 608
Fragments arranged by size:
1207 608 453 185 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 279 618 1132 2574
Size: 201 78 339 514 1442
Fragments arranged by size:
1442 514 339 201 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2137 2574
Size: 2137 437
EcoO109I rG'GnC_Cy
Cuts at: 0 4 5 832 1422 1635 2574
Size: 4 1 827 590 213 939
Fragments arranged by size:
939 827 590 213 4 1
EcoRI G'AATT_C
Cuts at: 0 718 2574
Size: 718 1856
EcoRV GAT'ATC
Cuts at: 0 1062 1866 2574
Size: 1062 804 708
Fragments arranged by size:
1062 804 708
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1809 2574
Size: 1809 765
HaeII r_GCGC'y
Cuts at: 0 149 549 645 2574
Size: 149 400 96 1929
Fragments arranged by size:
1929 400 149 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 406 438 1642 1674 2286 2318
Size: 277 32 97 32 1204 32 612 32
Cuts at: 2318 2538 2570 2574
Size: 220 32 4
Fragments arranged by size:
1204 612 277 220 97 32 32 32
32 32 4
HincII GTy'rAC
Cuts at: 0 237 1734 1770 1917 2574
Size: 237 1497 36 147 657
Fragments arranged by size:
1497 657 237 147 36
MscI TGG'CCA
Cuts at: 0 272 700 1309 2113 2158 2574
Size: 272 428 609 804 45 416
Fragments arranged by size:
804 609 428 416 272 45
MslI CAynn'nnrTG
Cuts at: 0 1303 1567 2281 2574
Size: 1303 264 714 293
Fragments arranged by size:
1303 714 293 264
MspA1I CmG'CkG
Cuts at: 0 52 221 242 489 638 857 1035 1152
Size: 52 169 21 247 149 219 178 117
Cuts at: 1152 2574
Size: 1422
Fragments arranged by size:
1422 247 219 178 169 149 117 52
21
NaeI GCC'GGC
Cuts at: 0 757 2574
Size: 757 1817
NcoI C'CATG_G
Cuts at: 0 747 2574
Size: 747 1827
NgoMIV G'CCGG_C
Cuts at: 0 755 2574
Size: 755 1819
NsiI A_TGCA'T
Cuts at: 0 118 868 2574
Size: 118 750 1706
Fragments arranged by size:
1706 750 118
NspI r_CATG'y
Cuts at: 0 2106 2574
Size: 2106 468
PasI CC'CwG_GG
Cuts at: 0 8 2269 2574
Size: 8 2261 305
Fragments arranged by size:
2261 305 8
PflMI CCAn_nnn'nTGG
Cuts at: 0 1038 2313 2574
Size: 1038 1275 261
Fragments arranged by size:
1275 1038 261
PpuMI rG'GwC_Cy
Cuts at: 0 832 2574
Size: 832 1742
PspOMI G'GGCC_C
Cuts at: 0 4 2574
Size: 4 2570
PstI C_TGCA'G
Cuts at: 0 66 1093 1357 2154 2574
Size: 66 1027 264 797 420
Fragments arranged by size:
1027 797 420 264 66
PvuII CAG'CTG
Cuts at: 0 221 489 638 1035 1152 2574
Size: 221 268 149 397 117 1422
Fragments arranged by size:
1422 397 268 221 149 117
SacII CC_GC'GG
Cuts at: 0 53 2574
Size: 53 2521
SanDI GG'GwC_CC
Cuts at: 0 832 2574
Size: 832 1742
SfcI C'TryA_G
Cuts at: 0 62 549 1089 1353 2150 2532 2574
Size: 62 487 540 264 797 382 42
Fragments arranged by size:
797 540 487 382 264 62 42
SmlI C'TyrA_G
Cuts at: 0 994 1755 2443 2574
Size: 994 761 688 131
Fragments arranged by size:
994 761 688 131
SphI G_CATG'C
Cuts at: 0 2106 2574
Size: 2106 468
StuI AGG'CCT
Cuts at: 0 421 1171 2574
Size: 421 750 1403
Fragments arranged by size:
1403 750 421
StyI C'CwwG_G
Cuts at: 0 570 747 1508 1555 1829 2574
Size: 570 177 761 47 274 745
Fragments arranged by size:
761 745 570 274 177 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1375 1486 1990 2176 2574
Size: 1375 111 504 186 398
Fragments arranged by size:
1375 504 398 186 111
TatI w'GTAC_w
Cuts at: 0 1253 1592 2574
Size: 1253 339 982
Fragments arranged by size:
1253 982 339
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 429 461 1810 1842 2574
Size: 429 32 1349 32 732
Fragments arranged by size:
1349 732 429 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 880 1399 1477 2298 2574
Size: 880 519 78 821 276
Fragments arranged by size:
880 821 519 276 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 613 730 951 2574
Size: 613 117 221 1623
Fragments arranged by size:
1623 613 221 117
XmnI GAAnn'nnTTC
Cuts at: 0 1676 2574
Size: 1676 898
Enzymes that do cut:
AarI AclI AcuI AfeI AflII AflIII AleI AlwNI
ApaI ApoI BamHI BanII BbsI BbvCI BciVI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI EaeI
EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PflMI PpuMI PspOMI PstI PvuII
SacII SanDI SfcI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AgeI AhdI AlfI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI
BcgI BclI BlpI BmtI BsaI BsaBI BsgI BsiEI
BsiWI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I CspCI DraI DraIII EagI EciI EcoICRI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI XmaI ZraI