(Linear) (Six-base) MAPSORT of: KIBB4388.seq  Check: 2276  from: 1  to: 1047



KIBB4388.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     678    1047 
   Size:        355     323     369

  Fragments arranged by size: 

                 369     355     323
 
AfeI AGC'GCT

Cuts at:      0     745    1047 
   Size:        745     302
 
AlwNI CAG_nnn'CTG

Cuts at:      0     893    1047 
   Size:        893     154
 
ApaLI G'TGCA_C

Cuts at:      0     164    1047 
   Size:        164     883
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     471    1047 
   Size:        471     576
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     831     865    1047 
   Size:        831      34     182

  Fragments arranged by size: 

                 831     182      34
 
Bme1580I G_kGCm'C

Cuts at:      0     168    1047 
   Size:        168     879
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     510     725    1047 
   Size:        510     215     322

  Fragments arranged by size: 

                 510     322     215
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     450    1047 
   Size:        450     597
 
BsaBI GATnn'nnATC

Cuts at:      0      20    1047 
   Size:         20    1027
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     547     577    1047 
   Size:        547      30     470

  Fragments arranged by size: 

                 547     470      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     256     304    1047 
   Size:        256      48     743

  Fragments arranged by size: 

                 743     256      48
 
BseYI C'CCAG_C

Cuts at:      0     603    1047 
   Size:        603     444
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     745    1047 
   Size:        745     302
 
BsiHKAI G_wGCw'C

Cuts at:      0     168    1047 
   Size:        168     879
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     348    1047 
   Size:        348     699
 
Bsp1286I G_dGCh'C

Cuts at:      0     168    1047 
   Size:        168     879
 
BsrDI GCAATG_nn'

Cuts at:      0     383    1047 
   Size:        383     664
 
BstBI TT'CG_AA

Cuts at:      0     765    1047 
   Size:        765     282
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     313    1047 
   Size:        313     734
 
BtgI C'CryG_G

Cuts at:      0     347     752    1047 
   Size:        347     405     295

  Fragments arranged by size: 

                 405     347     295
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      22      57     855     890    1047 
   Size:         22      35     798      35     157

  Fragments arranged by size: 

                 798     157      35      35      22
 
EaeI y'GGCC_r

Cuts at:      0     834    1047 
   Size:        834     213
 
EarI CTCTTCn'nnn_

Cuts at:      0     331    1047 
   Size:        331     716
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     450     678    1047 
   Size:        355      95     228     369

  Fragments arranged by size: 

                 369     355     228      95
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1024    1047 
   Size:       1024      23
 
EcoRV GAT'ATC

Cuts at:      0     517     672    1047 
   Size:        517     155     375

  Fragments arranged by size: 

                 517     375     155
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1045    1047 
   Size:       1045       2
 
HaeII r_GCGC'y

Cuts at:      0     747     951    1047 
   Size:        747     204      96

  Fragments arranged by size: 

                 747     204      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1028    1047 
   Size:       1028      19
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     800     944    1047 
   Size:        800     144     103

  Fragments arranged by size: 

                 800     144     103
 
MscI TGG'CCA

Cuts at:      0     836    1047 
   Size:        836     211
 
MslI CAynn'nnrTG

Cuts at:      0     142     524     586    1047 
   Size:        142     382      62     461

  Fragments arranged by size: 

                 461     382     142      62
 
NcoI C'CATG_G

Cuts at:      0     347     752    1047 
   Size:        347     405     295

  Fragments arranged by size: 

                 405     347     295
 
NdeI CA'TA_TG

Cuts at:      0     216    1047 
   Size:        216     831
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     210    1047 
   Size:        210     837
 
PshAI GACnn'nnGTC

Cuts at:      0     475    1047 
   Size:        475     572
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     314     346    1047 
   Size:        314      32     701

  Fragments arranged by size: 

                 701     314      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0     331    1047 
   Size:        331     716
 
SfcI C'TryA_G

Cuts at:      0     989    1047 
   Size:        989      58
 
SpeI A'CTAG_T

Cuts at:      0     772    1047 
   Size:        772     275
 
StuI AGG'CCT

Cuts at:      0     730    1047 
   Size:        730     317
 
StyI C'CwwG_G

Cuts at:      0      11     347     752    1047 
   Size:         11     336     405     295

  Fragments arranged by size: 

                 405     336     295      11
 
TatI w'GTAC_w

Cuts at:      0     967    1047 
   Size:        967      80
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     547     579    1047 
   Size:        547      32     468

  Fragments arranged by size: 

                 547     468      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     354    1047 
   Size:        354     693
 

 Enzymes that do cut:

     AcuI     AfeI    AlwNI    ApaLI     BbsI     BcgI Bme1580I     BmrI
     BpmI    BsaBI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI    BsmBI
 Bsp1286I    BsrDI    BstBI    BstXI     BtgI    CspCI     EaeI     EarI
  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I     MmeI     MscI
     MslI     NcoI     NdeI    PflMI    PshAI     PsrI     SapI     SfcI
     SpeI     StuI     StyI     TatI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstYI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI   MspA1I     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     SwaI    TaqII
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI