Restriction map for
pF1KB4393
(Linear) (Six-base) MAPSORT of: KIBB4393.seq Check: 7469 from: 1 to: 4170
KIBB4393.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3157 4170
Size: 3157 1013
AccI GT'mk_AC
Cuts at: 0 2066 2576 4170
Size: 2066 510 1594
Fragments arranged by size:
2066 1594 510
Acc65I G'GTAC_C
Cuts at: 0 490 4170
Size: 490 3680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 394 565 716 1054 1440 1785 2149
Size: 276 118 171 151 338 386 345 364
Cuts at: 2149 2278 3169 4170
Size: 129 891 1001
Fragments arranged by size:
1001 891 386 364 345 338 276 171
151 129 118
AleI CACnn'nnGTG
Cuts at: 0 3823 4170
Size: 3823 347
AlwNI CAG_nnn'CTG
Cuts at: 0 375 2297 3091 3573 4170
Size: 375 1922 794 482 597
Fragments arranged by size:
1922 794 597 482 375
ApoI r'AATT_y
Cuts at: 0 573 672 1163 2911 4147 4170
Size: 573 99 491 1748 1236 23
Fragments arranged by size:
1748 1236 573 491 99 23
AvaI C'yCGr_G
Cuts at: 0 975 1593 2420 4170
Size: 975 618 827 1750
Fragments arranged by size:
1750 975 827 618
AvrII C'CTAG_G
Cuts at: 0 1962 4170
Size: 1962 2208
BamHI G'GATC_C
Cuts at: 0 558 1972 4170
Size: 558 1414 2198
Fragments arranged by size:
2198 1414 558
BanI G'GyrC_C
Cuts at: 0 490 768 817 996 1642 3133 3727 4170
Size: 490 278 49 179 646 1491 594 443
Fragments arranged by size:
1491 646 594 490 443 278 179 49
BanII G_rGCy'C
Cuts at: 0 974 1331 2990 3550 4076 4170
Size: 974 357 1659 560 526 94
Fragments arranged by size:
1659 974 560 526 357 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 2784 3157 3973 4170
Size: 2784 373 816 197
Fragments arranged by size:
2784 816 373 197
BbvCI CC'TCA_GC
Cuts at: 0 2231 4170
Size: 2231 1939
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3825 3859 4170
Size: 3825 34 311
Fragments arranged by size:
3825 311 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2599 4170
Size: 2599 1571
BclI T'GATC_A
Cuts at: 0 914 4170
Size: 914 3256
BglI GCCn_nnn'nGGC
Cuts at: 0 2056 4170
Size: 2056 2114
BglII A'GATC_T
Cuts at: 0 1796 1887 2640 4170
Size: 1796 91 753 1530
Fragments arranged by size:
1796 1530 753 91
Bme1580I G_kGCm'C
Cuts at: 0 642 954 3075 3114 3203 3730 4170
Size: 642 312 2121 39 89 527 440
Fragments arranged by size:
2121 642 527 440 312 89 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 936 2019 2646 2798 4170
Size: 936 1083 627 152 1372
Fragments arranged by size:
1372 1083 936 627 152
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1081 4170
Size: 1081 3089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 2263 3064 4170
Size: 283 1980 801 1106
Fragments arranged by size:
1980 1106 801 283
Bpu10I CC'TnA_GC
Cuts at: 0 2231 4170
Size: 2231 1939
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1230 1307 1315 1645 4170
Size: 1230 77 8 330 2525
Fragments arranged by size:
2525 1230 330 77 8
BsaI GGTCTCn'nnnn_
Cuts at: 0 2230 2931 3040 4170
Size: 2230 701 109 1130
Fragments arranged by size:
2230 1130 701 109
BsaAI yAC'GTr
Cuts at: 0 3561 3858 4170
Size: 3561 297 312
Fragments arranged by size:
3561 312 297
BsaBI GATnn'nnATC
Cuts at: 0 1829 4170
Size: 1829 2341
BsaWI w'CCGG_w
Cuts at: 0 1124 1448 2518 4170
Size: 1124 324 1070 1652
Fragments arranged by size:
1652 1124 1070 324
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 256 286 1361 1391 4170
Size: 256 30 1075 30 2779
Fragments arranged by size:
2779 1075 256 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 373 1642 2248 2723 4170
Size: 91 282 1269 606 475 1447
Fragments arranged by size:
1447 1269 606 475 282 91
BseYI C'CCAG_C
Cuts at: 0 630 690 2481 4170
Size: 630 60 1791 1689
Fragments arranged by size:
1791 1689 630 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1387 2208 3444 3622 4170
Size: 1387 821 1236 178 548
Fragments arranged by size:
1387 1236 821 548 178
BsiEI CG_ry'CG
Cuts at: 0 2562 4170
Size: 2562 1608
BsiHKAI G_wGCw'C
Cuts at: 0 167 974 1331 3105 3550 4170
Size: 167 807 357 1774 445 620
Fragments arranged by size:
1774 807 620 445 357 167
BsmI GAATG_Cn'
Cuts at: 0 980 2157 3608 4170
Size: 980 1177 1451 562
Fragments arranged by size:
1451 1177 980 562
BsmBI CGTCTCn'nnnn_
Cuts at: 0 16 4170
Size: 16 4154
Bsp1286I G_dGCh'C
Cuts at: 0 167 642 954 974 1331 2990 3075 3105
Size: 167 475 312 20 357 1659 85 30
Cuts at: 3105 3114 3203 3550 3730 4076 4170
Size: 9 89 347 180 346 94
Fragments arranged by size:
1659 475 357 347 346 312 180 167
94 89 85 30 20 9
BspEI T'CCGG_A
Cuts at: 0 1124 4170
Size: 1124 3046
BspHI T'CATG_A
Cuts at: 0 525 2855 3878 4170
Size: 525 2330 1023 292
Fragments arranged by size:
2330 1023 525 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 340 1856 3157 3701 4170
Size: 340 1516 1301 544 469
Fragments arranged by size:
1516 1301 544 469 340
BsrDI GCAATG_nn'
Cuts at: 0 1700 4170
Size: 1700 2470
BsrFI r'CCGG_y
Cuts at: 0 2155 2557 4170
Size: 2155 402 1613
Fragments arranged by size:
2155 1613 402
BsrGI T'GTAC_A
Cuts at: 0 122 1477 4170
Size: 122 1355 2693
Fragments arranged by size:
2693 1355 122
BssSI C'ACGA_G
Cuts at: 0 3298 4170
Size: 3298 872
BstAPI GCAn_nnn'nTGC
Cuts at: 0 332 2297 3100 3861 4170
Size: 332 1965 803 761 309
Fragments arranged by size:
1965 803 761 332 309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 3121 3597 4170
Size: 475 2646 476 573
Fragments arranged by size:
2646 573 476 475
BstYI r'GATC_y
Cuts at: 0 558 890 1796 1887 1972 2640 2721 3582
Size: 558 332 906 91 85 668 81 861
Cuts at: 3582 4170
Size: 588
Fragments arranged by size:
906 861 668 588 558 332 91 85
81
Bsu36I CC'TnA_GG
Cuts at: 0 74 1235 1813 4170
Size: 74 1161 578 2357
Fragments arranged by size:
2357 1161 578 74
BtgI C'CryG_G
Cuts at: 0 2079 2336 3821 3831 4170
Size: 2079 257 1485 10 339
Fragments arranged by size:
2079 1485 339 257 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1838 4170
Size: 1838 2332
BtsI GCAGTG_nn'
Cuts at: 0 952 2225 2298 3101 4170
Size: 952 1273 73 803 1069
Fragments arranged by size:
1273 1069 952 803 73
ClaI AT'CG_AT
Cuts at: 0 1364 4170
Size: 1364 2806
DraI TTT'AAA
Cuts at: 0 681 2763 4170
Size: 681 2082 1407
Fragments arranged by size:
2082 1407 681
DraIII CAC_nnn'GTG
Cuts at: 0 1781 3718 3720 4170
Size: 1781 1937 2 450
Fragments arranged by size:
1937 1781 450 2
DrdI GACnn_nn'nnGTC
Cuts at: 0 2847 4170
Size: 2847 1323
EaeI y'GGCC_r
Cuts at: 0 32 1157 1430 2115 2555 2559 3707 3923
Size: 32 1125 273 685 440 4 1148 216
Cuts at: 3923 4170
Size: 247
Fragments arranged by size:
1148 1125 685 440 273 247 216 32
4
EagI C'GGCC_G
Cuts at: 0 2559 4170
Size: 2559 1611
EarI CTCTTCn'nnn_
Cuts at: 0 2857 4081 4170
Size: 2857 1224 89
Fragments arranged by size:
2857 1224 89
EcoICRI GAG'CTC
Cuts at: 0 972 1329 3548 4170
Size: 972 357 2219 622
Fragments arranged by size:
2219 972 622 357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 283 394 565 716 1054 1440 1785
Size: 276 7 111 171 151 338 386 345
Cuts at: 1785 2149 2263 2278 3064 3169 4170
Size: 364 114 15 786 105 1001
Fragments arranged by size:
1001 786 386 364 345 338 276 171
151 114 111 105 15 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2117 3577 4170
Size: 2117 1460 593
Fragments arranged by size:
2117 1460 593
EcoO109I rG'GnC_Cy
Cuts at: 0 1554 2447 4104 4124 4170
Size: 1554 893 1657 20 46
Fragments arranged by size:
1657 1554 893 46 20
EcoRI G'AATT_C
Cuts at: 0 4147 4170
Size: 4147 23
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1600 1815 3379 4170
Size: 1600 215 1564 791
Fragments arranged by size:
1600 1564 791 215
FseI GG_CCGG'CC
Cuts at: 0 2561 4170
Size: 2561 1609
HaeII r_GCGC'y
Cuts at: 0 482 4170
Size: 482 3688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 796 828 1007 1039 1346 1378 2023 2055
Size: 796 32 179 32 307 32 645 32
Cuts at: 2055 2632 2664 3440 3472 3617 3649 4170
Size: 577 32 776 32 145 32 521
Fragments arranged by size:
796 776 645 577 521 307 179 145
32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 2577 4170
Size: 2577 1593
HindIII A'AGCT_T
Cuts at: 0 3670 4170
Size: 3670 500
KpnI G_GTAC'C
Cuts at: 0 494 4170
Size: 494 3676
MfeI C'AATT_G
Cuts at: 0 2090 4170
Size: 2090 2080
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1050 3896 4170
Size: 1050 2846 274
Fragments arranged by size:
2846 1050 274
MscI TGG'CCA
Cuts at: 0 34 1159 1432 2117 3709 3925 4170
Size: 34 1125 273 685 1592 216 245
Fragments arranged by size:
1592 1125 685 273 245 216 34
MslI CAynn'nnrTG
Cuts at: 0 473 3139 3823 4042 4170
Size: 473 2666 684 219 128
Fragments arranged by size:
2666 684 473 219 128
MspA1I CmG'CkG
Cuts at: 0 694 942 1611 2485 2596 3006 4170
Size: 694 248 669 874 111 410 1164
Fragments arranged by size:
1164 874 694 669 410 248 111
NaeI GCC'GGC
Cuts at: 0 2157 2559 4170
Size: 2157 402 1611
Fragments arranged by size:
2157 1611 402
NcoI C'CATG_G
Cuts at: 0 2336 3821 4170
Size: 2336 1485 349
Fragments arranged by size:
2336 1485 349
NgoMIV G'CCGG_C
Cuts at: 0 2155 2557 4170
Size: 2155 402 1613
Fragments arranged by size:
2155 1613 402
NspI r_CATG'y
Cuts at: 0 145 2613 4170
Size: 145 2468 1557
Fragments arranged by size:
2468 1557 145
PasI CC'CwG_GG
Cuts at: 0 3192 4170
Size: 3192 978
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 1007 2553 4170
Size: 177 830 1546 1617
Fragments arranged by size:
1617 1546 830 177
PfoI T'CCnGG_A
Cuts at: 0 17 2303 4170
Size: 17 2286 1867
Fragments arranged by size:
2286 1867 17
PmlI CAC'GTG
Cuts at: 0 3858 4170
Size: 3858 312
PpuMI rG'GwC_Cy
Cuts at: 0 2447 4104 4170
Size: 2447 1657 66
Fragments arranged by size:
2447 1657 66
PstI C_TGCA'G
Cuts at: 0 941 947 2031 2210 2730 2900 3096 3569
Size: 941 6 1084 179 520 170 196 473
Cuts at: 3569 4170
Size: 601
Fragments arranged by size:
1084 941 601 520 473 196 179 170
6
PvuII CAG'CTG
Cuts at: 0 694 942 2485 2596 3006 4170
Size: 694 248 1543 111 410 1164
Fragments arranged by size:
1543 1164 694 410 248 111
SacI G_AGCT'C
Cuts at: 0 974 1331 3550 4170
Size: 974 357 2219 620
Fragments arranged by size:
2219 974 620 357
SalI G'TCGA_C
Cuts at: 0 2575 4170
Size: 2575 1595
SanDI GG'GwC_CC
Cuts at: 0 4104 4170
Size: 4104 66
SapI GCTCTTCn'nnn_
Cuts at: 0 4081 4170
Size: 4081 89
SbfI CC_TGCA'GG
Cuts at: 0 2900 4170
Size: 2900 1270
ScaI AGT'ACT
Cuts at: 0 1870 4170
Size: 1870 2300
SfcI C'TryA_G
Cuts at: 0 937 943 2027 2206 2726 2896 3051 3092
Size: 937 6 1084 179 520 170 155 41
Cuts at: 3092 3565 4170
Size: 473 605
Fragments arranged by size:
1084 937 605 520 473 179 170 155
41 6
SmlI C'TyrA_G
Cuts at: 0 1209 1322 1330 1660 2420 4170
Size: 1209 113 8 330 760 1750
Fragments arranged by size:
1750 1209 760 330 113 8
SspI AAT'ATT
Cuts at: 0 1986 4170
Size: 1986 2184
StuI AGG'CCT
Cuts at: 0 1818 4170
Size: 1818 2352
StyI C'CwwG_G
Cuts at: 0 1685 1856 1962 2118 2336 3230 3590 3821
Size: 1685 171 106 156 218 894 360 231
Cuts at: 3821 4170
Size: 349
Fragments arranged by size:
1685 894 360 349 231 218 171 156
106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1014 1259 3040 3088 3151 4170
Size: 1014 245 1781 48 63 1019
Fragments arranged by size:
1781 1019 1014 245 63 48
TatI w'GTAC_w
Cuts at: 0 122 1477 1868 2177 3062 3554 3665 3815
Size: 122 1355 391 309 885 492 111 150
Cuts at: 3815 4170
Size: 355
Fragments arranged by size:
1355 885 492 391 355 309 150 122
111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 256 288 1064 1096 1361 1393 4003 4035
Size: 256 32 776 32 265 32 2610 32
Cuts at: 4035 4170
Size: 135
Fragments arranged by size:
2610 776 265 256 135 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 55 549 2496 2701 4170
Size: 55 494 1947 205 1469
Fragments arranged by size:
1947 1469 494 205 55
XhoI C'TCGA_G
Cuts at: 0 2420 4170
Size: 2420 1750
XmnI GAAnn'nnTTC
Cuts at: 0 701 3038 3154 3359 4170
Size: 701 2337 116 205 811
Fragments arranged by size:
2337 811 701 205 116
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AlwNI ApoI AvaI
AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI HaeII Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PmlI PpuMI PstI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SmlI SspI
StuI StyI TaqII TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AloI ApaI ApaLI AscI AseI AsiSI BaeI BbeI
BfrBI BlpI BmgBI BmtI BsaHI BsiWI BsrBI BssHII
BstBI BstEII BstZ17I CspCI EciI EcoRV FspI FspAI
HpaI KasI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XmaI ZraI