Restriction map for
pF1KB4394
(Linear) (Six-base) MAPSORT of: KIBB4394.seq Check: 3470 from: 1 to: 2502
KIBB4394.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 2162 2502
Size: 2162 340
Acc65I G'GTAC_C
Cuts at: 0 911 2502
Size: 911 1591
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1776 1802 2154 2335 2502
Size: 110 1666 26 352 181 167
Fragments arranged by size:
1666 352 181 167 110 26
AflIII A'CryG_T
Cuts at: 0 1518 2502
Size: 1518 984
AleI CACnn'nnGTG
Cuts at: 0 465 2294 2502
Size: 465 1829 208
Fragments arranged by size:
1829 465 208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 770 2204 2502
Size: 770 1434 298
Fragments arranged by size:
1434 770 298
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 1744 1776 2502
Size: 136 32 1576 32 726
Fragments arranged by size:
1576 726 136 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 162 738 1098 1216 2205 2391 2502
Size: 162 576 360 118 989 186 111
Fragments arranged by size:
989 576 360 186 162 118 111
ApaI G_GGCC'C
Cuts at: 0 1926 2143 2502
Size: 1926 217 359
Fragments arranged by size:
1926 359 217
ApaLI G'TGCA_C
Cuts at: 0 384 2285 2502
Size: 384 1901 217
Fragments arranged by size:
1901 384 217
ApoI r'AATT_y
Cuts at: 0 1951 2502
Size: 1951 551
AvaI C'yCGr_G
Cuts at: 0 76 1571 1968 2011 2125 2136 2228 2502
Size: 76 1495 397 43 114 11 92 274
Fragments arranged by size:
1495 397 274 114 92 76 43 11
AvrII C'CTAG_G
Cuts at: 0 288 2502
Size: 288 2214
BanI G'GyrC_C
Cuts at: 0 276 341 538 911 1498 2395 2502
Size: 276 65 197 373 587 897 107
Fragments arranged by size:
897 587 373 276 197 107 65
BanII G_rGCy'C
Cuts at: 0 62 135 674 876 1091 1369 1447 1858
Size: 62 73 539 202 215 278 78 411
Cuts at: 1858 1926 2143 2502
Size: 68 217 359
Fragments arranged by size:
539 411 359 278 217 215 202 78
73 68 62
BbeI G_GCGC'C
Cuts at: 0 280 345 1502 2502
Size: 280 65 1157 1000
Fragments arranged by size:
1157 1000 280 65
BbsI GAAGACnn'nnnn_
Cuts at: 0 1458 1550 1679 2502
Size: 1458 92 129 823
Fragments arranged by size:
1458 823 129 92
BbvCI CC'TCA_GC
Cuts at: 0 1156 1843 2234 2502
Size: 1156 687 391 268
Fragments arranged by size:
1156 687 391 268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 189 223 1636 1670 2502
Size: 189 34 1413 34 832
Fragments arranged by size:
1413 832 189 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1203 2502
Size: 1203 1299
BclI T'GATC_A
Cuts at: 0 1529 2502
Size: 1529 973
BglI GCCn_nnn'nGGC
Cuts at: 0 803 1507 2098 2502
Size: 803 704 591 404
Fragments arranged by size:
803 704 591 404
Bme1580I G_kGCm'C
Cuts at: 0 199 265 388 1309 1926 2012 2143 2289
Size: 199 66 123 921 617 86 131 146
Cuts at: 2289 2398 2502
Size: 109 104
Fragments arranged by size:
921 617 199 146 131 123 109 104
86 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 1296 2502
Size: 1296 1206
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 102 1983 2502
Size: 102 1881 519
Fragments arranged by size:
1881 519 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 913 1594 1840 1948 2502
Size: 262 651 681 246 108 554
Fragments arranged by size:
681 651 554 262 246 108
Bpu10I CC'TnA_GC
Cuts at: 0 231 875 1156 1843 2234 2502
Size: 231 644 281 687 391 268
Fragments arranged by size:
687 644 391 281 268 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 658 1684 2017 2502
Size: 460 198 1026 333 485
Fragments arranged by size:
1026 485 460 333 198
BsaI GGTCTCn'nnnn_
Cuts at: 0 73 814 2136 2502
Size: 73 741 1322 366
Fragments arranged by size:
1322 741 366 73
BsaAI yAC'GTr
Cuts at: 0 867 1488 2502
Size: 867 621 1014
Fragments arranged by size:
1014 867 621
BsaHI Gr'CG_yC
Cuts at: 0 247 277 342 722 1499 2502
Size: 247 30 65 380 777 1003
Fragments arranged by size:
1003 777 380 247 65 30
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 54 84 136 142 166 172 1339 1369
Size: 54 30 52 6 24 6 1167 30
Cuts at: 1369 1552 1582 2502
Size: 183 30 920
Fragments arranged by size:
1167 920 183 54 52 30 30 30
24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 163 427 727 841 943 1099 1351
Size: 160 3 264 300 114 102 156 252
Cuts at: 1351 2113 2502
Size: 762 389
Fragments arranged by size:
762 389 300 264 252 160 156 114
102 3
BseYI C'CCAG_C
Cuts at: 0 32 103 109 115 801 945 1387 1624
Size: 32 71 6 6 686 144 442 237
Cuts at: 1624 2144 2153 2366 2492 2502
Size: 520 9 213 126 10
Fragments arranged by size:
686 520 442 237 213 144 126 71
32 10 9 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 314 1018 1311 2230 2502
Size: 202 112 704 293 919 272
Fragments arranged by size:
919 704 293 272 202 112
BsiEI CG_ry'CG
Cuts at: 0 407 791 2502
Size: 407 384 1711
Fragments arranged by size:
1711 407 384
BsiHKAI G_wGCw'C
Cuts at: 0 232 388 674 1369 1858 2289 2502
Size: 232 156 286 695 489 431 213
Fragments arranged by size:
695 489 431 286 232 213 156
Bsp1286I G_dGCh'C
Cuts at: 0 62 135 199 232 265 388 674 876
Size: 62 73 64 33 33 123 286 202
Cuts at: 876 1091 1309 1369 1447 1858 1926 2012 2143
Size: 215 218 60 78 411 68 86 131
Cuts at: 2143 2289 2398 2502
Size: 146 109 104
Fragments arranged by size:
411 286 218 215 202 146 131 123
109 104 86 78 73 68 64 62
60 33 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2390 2502
Size: 2390 112
BsrDI GCAATG_nn'
Cuts at: 0 181 2502
Size: 181 2321
BsrFI r'CCGG_y
Cuts at: 0 356 2300 2502
Size: 356 1944 202
Fragments arranged by size:
1944 356 202
BssHII G'CGCG_C
Cuts at: 0 644 809 989 2502
Size: 644 165 180 1513
Fragments arranged by size:
1513 644 180 165
BssSI C'ACGA_G
Cuts at: 0 481 639 2502
Size: 481 158 1863
Fragments arranged by size:
1863 481 158
BstAPI GCAn_nnn'nTGC
Cuts at: 0 260 392 608 738 2502
Size: 260 132 216 130 1764
Fragments arranged by size:
1764 260 216 132 130
BstXI CCAn_nnnn'nTGG
Cuts at: 0 945 1423 2502
Size: 945 478 1079
Fragments arranged by size:
1079 945 478
BstYI r'GATC_y
Cuts at: 0 695 2502
Size: 695 1807
Bsu36I CC'TnA_GG
Cuts at: 0 1633 2502
Size: 1633 869
BtgI C'CryG_G
Cuts at: 0 2084 2469 2502
Size: 2084 385 33
Fragments arranged by size:
2084 385 33
BtsI GCAGTG_nn'
Cuts at: 0 265 1599 2188 2502
Size: 265 1334 589 314
Fragments arranged by size:
1334 589 314 265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2442 2477 2502
Size: 2442 35 25
Fragments arranged by size:
2442 35 25
DraIII CAC_nnn'GTG
Cuts at: 0 227 2262 2502
Size: 227 2035 240
Fragments arranged by size:
2035 240 227
DrdI GACnn_nn'nnGTC
Cuts at: 0 1255 2502
Size: 1255 1247
EaeI y'GGCC_r
Cuts at: 0 404 788 795 935 1490 2099 2466 2502
Size: 404 384 7 140 555 609 367 36
Fragments arranged by size:
609 555 404 384 367 140 36 7
EagI C'GGCC_G
Cuts at: 0 404 788 2502
Size: 404 384 1714
Fragments arranged by size:
1714 404 384
EarI CTCTTCn'nnn_
Cuts at: 0 1276 2502
Size: 1276 1226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 199 2502
Size: 199 2303
EcoICRI GAG'CTC
Cuts at: 0 672 1367 1856 2502
Size: 672 695 489 646
Fragments arranged by size:
695 672 646 489
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 262 913 1594 1776 1802 1840 1948
Size: 110 152 651 681 182 26 38 108
Cuts at: 1948 2154 2335 2502
Size: 206 181 167
Fragments arranged by size:
681 651 206 182 181 167 152 110
108 38 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1057 1078 2125 2209 2416 2502
Size: 1057 21 1047 84 207 86
Fragments arranged by size:
1057 1047 207 86 84 21
EcoO109I rG'GnC_Cy
Cuts at: 0 29 39 1152 1233 1621 1638 1897 1923
Size: 29 10 1113 81 388 17 259 26
Cuts at: 1923 2140 2310 2486 2502
Size: 217 170 176 16
Fragments arranged by size:
1113 388 259 217 176 170 81 29
26 17 16 10
EcoRI G'AATT_C
Cuts at: 0 1951 2502
Size: 1951 551
FspI TGC'GCA
Cuts at: 0 374 508 1114 2502
Size: 374 134 606 1388
Fragments arranged by size:
1388 606 374 134
FspAI rTGC'GCAy
Cuts at: 0 1114 2502
Size: 1114 1388
HaeII r_GCGC'y
Cuts at: 0 280 345 739 1066 1502 2084 2431 2502
Size: 280 65 394 327 436 582 347 71
Fragments arranged by size:
582 436 394 347 327 280 71 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 924 956 1144 1176 2502
Size: 924 32 188 32 1326
Fragments arranged by size:
1326 924 188 32 32
HincII GTy'rAC
Cuts at: 0 1248 2502
Size: 1248 1254
KasI G'GCGC_C
Cuts at: 0 276 341 1498 2502
Size: 276 65 1157 1004
Fragments arranged by size:
1157 1004 276 65
KpnI G_GTAC'C
Cuts at: 0 915 2502
Size: 915 1587
MluI A'CGCG_T
Cuts at: 0 1518 2502
Size: 1518 984
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 2396 2502
Size: 593 1803 106
Fragments arranged by size:
1803 593 106
MscI TGG'CCA
Cuts at: 0 937 1492 2101 2502
Size: 937 555 609 401
Fragments arranged by size:
937 609 555 401
MslI CAynn'nnrTG
Cuts at: 0 465 1324 1421 2294 2502
Size: 465 859 97 873 208
Fragments arranged by size:
873 859 465 208 97
MspA1I CmG'CkG
Cuts at: 0 339 392 630 801 1189 1614 1734 2148
Size: 339 53 238 171 388 425 120 414
Cuts at: 2148 2218 2502
Size: 70 284
Fragments arranged by size:
425 414 388 339 284 238 171 120
70 53
NarI GG'CG_CC
Cuts at: 0 277 342 1499 2502
Size: 277 65 1157 1003
Fragments arranged by size:
1157 1003 277 65
NcoI C'CATG_G
Cuts at: 0 2084 2502
Size: 2084 418
NspI r_CATG'y
Cuts at: 0 258 1044 2161 2502
Size: 258 786 1117 341
Fragments arranged by size:
1117 786 341 258
PasI CC'CwG_GG
Cuts at: 0 1173 1229 2502
Size: 1173 56 1273
Fragments arranged by size:
1273 1173 56
PflMI CCAn_nnn'nTGG
Cuts at: 0 2464 2502
Size: 2464 38
PfoI T'CCnGG_A
Cuts at: 0 530 1640 1955 1997 2502
Size: 530 1110 315 42 505
Fragments arranged by size:
1110 530 505 315 42
PmlI CAC'GTG
Cuts at: 0 867 1488 2502
Size: 867 621 1014
Fragments arranged by size:
1014 867 621
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 2502
Size: 136 32 2334
Fragments arranged by size:
2334 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 39 1152 1233 1638 2502
Size: 39 1113 81 405 864
Fragments arranged by size:
1113 864 405 81 39
PspOMI G'GGCC_C
Cuts at: 0 1922 2139 2502
Size: 1922 217 363
Fragments arranged by size:
1922 363 217
PspXI vC'TCGA_Gb
Cuts at: 0 2125 2502
Size: 2125 377
PstI C_TGCA'G
Cuts at: 0 338 743 971 1151 1835 2183 2201 2345
Size: 338 405 228 180 684 348 18 144
Cuts at: 2345 2387 2502
Size: 42 115
Fragments arranged by size:
684 405 348 338 228 180 144 115
42 18
PvuII CAG'CTG
Cuts at: 0 801 1189 1614 1734 2148 2218 2502
Size: 801 388 425 120 414 70 284
Fragments arranged by size:
801 425 414 388 284 120 70
SacI G_AGCT'C
Cuts at: 0 674 1369 1858 2502
Size: 674 695 489 644
Fragments arranged by size:
695 674 644 489
SanDI GG'GwC_CC
Cuts at: 0 1233 2502
Size: 1233 1269
SbfI CC_TGCA'GG
Cuts at: 0 1151 2387 2502
Size: 1151 1236 115
Fragments arranged by size:
1236 1151 115
SfcI C'TryA_G
Cuts at: 0 122 334 739 967 1147 1831 2179 2197
Size: 122 212 405 228 180 684 348 18
Cuts at: 2197 2341 2383 2502
Size: 144 42 119
Fragments arranged by size:
684 405 348 228 212 180 144 122
119 42 18
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 803 2502
Size: 803 1699
SfoI GGC'GCC
Cuts at: 0 278 343 1500 2502
Size: 278 65 1157 1002
Fragments arranged by size:
1157 1002 278 65
SmaI CCC'GGG
Cuts at: 0 2013 2138 2230 2502
Size: 2013 125 92 272
Fragments arranged by size:
2013 272 125 92
SmlI C'TyrA_G
Cuts at: 0 475 673 1699 2032 2125 2502
Size: 475 198 1026 333 93 377
Fragments arranged by size:
1026 475 377 333 198 93
SphI G_CATG'C
Cuts at: 0 258 2502
Size: 258 2244
StuI AGG'CCT
Cuts at: 0 2411 2502
Size: 2411 91
StyI C'CwwG_G
Cuts at: 0 288 1397 1805 2084 2502
Size: 288 1109 408 279 418
Fragments arranged by size:
1109 418 408 288 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 838 1186 2502
Size: 838 348 1316
Fragments arranged by size:
1316 838 348
TatI w'GTAC_w
Cuts at: 0 2321 2502
Size: 2321 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 54 86 178 210 2502
Size: 54 32 92 32 2292
Fragments arranged by size:
2292 92 54 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1492 2502
Size: 1492 1010
XhoI C'TCGA_G
Cuts at: 0 2125 2502
Size: 2125 377
XmaI C'CCGG_G
Cuts at: 0 2011 2136 2228 2502
Size: 2011 125 92 274
Fragments arranged by size:
2011 274 125 92
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NarI NcoI NspI PasI PflMI PfoI PmlI
PpiI PpuMI PspOMI PspXI PstI PvuII SacI SanDI
SbfI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AscI
AseI AsiSI BaeI BamHI BfrBI BglII BlpI BmgBI
BmtI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BstBI BstEII BstZ17I BtgZI ClaI DraI
EcoRV FalI FseI HindIII HpaI MfeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PshAI PsiI PsrI PvuI RsrII SacII SalI SapI
ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI Tth111I XbaI XmnI ZraI