(Linear) (Six-base) MAPSORT of: KIBB4401.seq  Check: 944  from: 1  to: 609



KIBB4401.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     609 
   Size:        431     178
 
AflIII A'CryG_T

Cuts at:      0     434     609 
   Size:        434     175
 
AleI CACnn'nnGTG

Cuts at:      0     535     609 
   Size:        535      74
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     532     564     609 
   Size:        532      32      45

  Fragments arranged by size: 

                 532      45      32
 
ApoI r'AATT_y

Cuts at:      0     415     609 
   Size:        415     194
 
AseI AT'TA_AT

Cuts at:      0     158     609 
   Size:        158     451
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      70     609 
   Size:         70     539
 
BbvCI CC'TCA_GC

Cuts at:      0     453     609 
   Size:        453     156
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     558     609 
   Size:        558      51
 
BglII A'GATC_T

Cuts at:      0     182     609 
   Size:        182     427
 
BmgBI CAC'GTC

Cuts at:      0      68     609 
   Size:         68     541
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     584     609 
   Size:        466     118      25

  Fragments arranged by size: 

                 466     118      25
 
Bpu10I CC'TnA_GC

Cuts at:      0     428     453     609 
   Size:        428      25     156

  Fragments arranged by size: 

                 428     156      25
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     442     609 
   Size:        442     167
 
BseYI C'CCAG_C

Cuts at:      0     398     609 
   Size:        398     211
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     187     609 
   Size:        187     422
 
BstYI r'GATC_y

Cuts at:      0     182     609 
   Size:        182     427
 
EarI CTCTTCn'nnn_

Cuts at:      0     490     609 
   Size:        490     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     466     584     609 
   Size:        431      35     118      25

  Fragments arranged by size: 

                 431     118      35      25
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     566     609 
   Size:        566      43
 
EcoRI G'AATT_C

Cuts at:      0     415     609 
   Size:        415     194
 
HaeII r_GCGC'y

Cuts at:      0     245     609 
   Size:        245     364
 
MfeI C'AATT_G

Cuts at:      0     122     609 
   Size:        122     487
 
MslI CAynn'nnrTG

Cuts at:      0     349     535     609 
   Size:        349     186      74

  Fragments arranged by size: 

                 349     186      74
 
NspI r_CATG'y

Cuts at:      0     438     503     609 
   Size:        438      65     106

  Fragments arranged by size: 

                 438     106      65
 
PciI A'CATG_T

Cuts at:      0     434     609 
   Size:        434     175
 
SfcI C'TryA_G

Cuts at:      0     178     609 
   Size:        178     431
 
SphI G_CATG'C

Cuts at:      0     503     609 
   Size:        503     106
 
StyI C'CwwG_G

Cuts at:      0     458     609 
   Size:        458     151
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     203     609 
   Size:        203     406
 
XmnI GAAnn'nnTTC

Cuts at:      0     484     609 
   Size:        484     125
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AloI     ApoI     AseI     BbsI    BbvCI
    BciVI    BglII    BmgBI     BpmI   Bpu10I     BsaI    BseYI    BspMI
    BstYI     EarI  Eco57MI    EcoNI    EcoRI    HaeII     MfeI     MslI
     NspI     PciI     SfcI     SphI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI    AlwNI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI