(Linear) (Six-base) MAPSORT of: KIBB4407.seq Check: 6732 from: 1 to: 5229 KIBB4407.orf With 182 enzymes: SgfI * January 29, 2016 11:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2566 3100 4146 5229 Size: 2566 534 1046 1083 Fragments arranged by size: 2566 1083 1046 534 AatII G_ACGT'C Cuts at: 0 3102 5229 Size: 3102 2127 AccI GT'mk_AC Cuts at: 0 802 5229 Size: 802 4427 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 854 966 1198 1250 1805 2219 3617 4907 Size: 854 112 232 52 555 414 1398 1290 Cuts at: 4907 5229 Size: 322 Fragments arranged by size: 1398 1290 854 555 414 322 232 112 52 AfeI AGC'GCT Cuts at: 0 5068 5229 Size: 5068 161 AflII C'TTAA_G Cuts at: 0 382 5229 Size: 382 4847 AflIII A'CryG_T Cuts at: 0 2863 3766 5229 Size: 2863 903 1463 Fragments arranged by size: 2863 1463 903 AhdI GACnn_n'nnGTC Cuts at: 0 1629 3843 4479 5229 Size: 1629 2214 636 750 Fragments arranged by size: 2214 1629 750 636 AleI CACnn'nnGTG Cuts at: 0 4006 5229 Size: 4006 1223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1432 2506 4178 4447 4645 5229 Size: 1432 1074 1672 269 198 584 Fragments arranged by size: 1672 1432 1074 584 269 198 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 4627 4659 5229 Size: 97 32 4498 32 570 Fragments arranged by size: 4498 570 97 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 814 2454 2558 2952 4737 5229 Size: 814 1640 104 394 1785 492 Fragments arranged by size: 1785 1640 814 492 394 104 ApaI G_GGCC'C Cuts at: 0 1516 5112 5229 Size: 1516 3596 117 Fragments arranged by size: 3596 1516 117 ApaLI G'TGCA_C Cuts at: 0 2612 5229 Size: 2612 2617 ApoI r'AATT_y Cuts at: 0 232 579 703 749 1283 1768 2571 2812 Size: 232 347 124 46 534 485 803 241 Cuts at: 2812 3284 5229 Size: 472 1945 Fragments arranged by size: 1945 803 534 485 472 347 241 232 124 46 AvaI C'yCGr_G Cuts at: 0 890 4467 4981 5229 Size: 890 3577 514 248 Fragments arranged by size: 3577 890 514 248 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 3876 3909 5229 Size: 3876 33 1320 Fragments arranged by size: 3876 1320 33 BamHI G'GATC_C Cuts at: 0 2727 5229 Size: 2727 2502 BanI G'GyrC_C Cuts at: 0 3226 4986 5229 Size: 3226 1760 243 Fragments arranged by size: 3226 1760 243 BanII G_rGCy'C Cuts at: 0 715 915 1516 3341 3877 4577 4982 5037 Size: 715 200 601 1825 536 700 405 55 Cuts at: 5037 5112 5229 Size: 75 117 Fragments arranged by size: 1825 715 700 601 536 405 200 117 75 55 BbeI G_GCGC'C Cuts at: 0 4990 5229 Size: 4990 239 BbsI GAAGACnn'nnnn_ Cuts at: 0 2143 4829 5229 Size: 2143 2686 400 Fragments arranged by size: 2686 2143 400 BbvCI CC'TCA_GC Cuts at: 0 596 3380 4397 5229 Size: 596 2784 1017 832 Fragments arranged by size: 2784 1017 832 596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 688 722 5229 Size: 688 34 4507 Fragments arranged by size: 4507 688 34 BciVI GTATCCnnnnn_n' Cuts at: 0 5178 5229 Size: 5178 51 BclI T'GATC_A Cuts at: 0 1676 3114 3453 4724 5229 Size: 1676 1438 339 1271 505 Fragments arranged by size: 1676 1438 1271 505 339 BfrBI ATG'CAT Cuts at: 0 2035 5229 Size: 2035 3194 BglI GCCn_nnn'nGGC Cuts at: 0 3997 5229 Size: 3997 1232 BglII A'GATC_T Cuts at: 0 1483 5229 Size: 1483 3746 BlpI GC'TnA_GC Cuts at: 0 2945 4555 5229 Size: 2945 1610 674 Fragments arranged by size: 2945 1610 674 Bme1580I G_kGCm'C Cuts at: 0 1516 2013 2616 5112 5229 Size: 1516 497 603 2496 117 Fragments arranged by size: 2496 1516 603 497 117 BmrI ACTGGGnnnn_n' Cuts at: 0 1451 2403 5229 Size: 1451 952 2826 Fragments arranged by size: 2826 1451 952 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4482 5085 5229 Size: 4482 603 144 Fragments arranged by size: 4482 603 144 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 929 3518 3873 4046 4246 4342 4540 4561 Size: 929 2589 355 173 200 96 198 21 Cuts at: 4561 4642 4654 4759 4996 5039 5227 5229 Size: 81 12 105 237 43 188 2 Fragments arranged by size: 2589 929 355 237 200 198 188 173 105 96 81 43 21 12 2 Bpu10I CC'TnA_GC Cuts at: 0 596 1742 3010 3303 3380 4397 5229 Size: 596 1146 1268 293 77 1017 832 Fragments arranged by size: 1268 1146 1017 832 596 293 77 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 188 2081 3829 5229 Size: 188 1893 1748 1400 Fragments arranged by size: 1893 1748 1400 188 BsaI GGTCTCn'nnnn_ Cuts at: 0 189 2063 3371 5229 Size: 189 1874 1308 1858 Fragments arranged by size: 1874 1858 1308 189 BsaAI yAC'GTr Cuts at: 0 1365 5229 Size: 1365 3864 BsaBI GATnn'nnATC Cuts at: 0 2732 3734 4540 5229 Size: 2732 1002 806 689 Fragments arranged by size: 2732 1002 806 689 BsaHI Gr'CG_yC Cuts at: 0 398 1336 1429 3099 3580 4505 4987 5042 Size: 398 938 93 1670 481 925 482 55 Cuts at: 5042 5229 Size: 187 Fragments arranged by size: 1670 938 925 482 481 398 187 93 55 BsaWI w'CCGG_w Cuts at: 0 1137 4587 5229 Size: 1137 3450 642 Fragments arranged by size: 3450 1137 642 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1119 1149 4252 4282 4627 4657 5012 5042 Size: 1119 30 3103 30 345 30 355 30 Cuts at: 5042 5229 Size: 187 Fragments arranged by size: 3103 1119 355 345 187 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 37 1044 1577 2737 3112 3586 3668 3671 Size: 37 1007 533 1160 375 474 82 3 Cuts at: 3671 3867 4243 4246 4471 4618 4705 4708 4711 Size: 196 376 3 225 147 87 3 3 Cuts at: 4711 4786 4876 4879 5229 Size: 75 90 3 350 Fragments arranged by size: 1160 1007 533 474 376 375 350 225 196 147 90 87 82 75 37 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 914 3648 4019 5229 Size: 914 2734 371 1210 Fragments arranged by size: 2734 1210 914 371 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1438 2224 2512 2734 2921 3549 4307 5229 Size: 1438 786 288 222 187 628 758 922 Fragments arranged by size: 1438 922 786 758 628 288 222 187 BsiEI CG_ry'CG Cuts at: 0 15 101 4418 4614 5229 Size: 15 86 4317 196 615 Fragments arranged by size: 4317 615 196 86 15 BsiHKAI G_wGCw'C Cuts at: 0 715 1606 2386 2616 3341 3877 4577 5229 Size: 715 891 780 230 725 536 700 652 Fragments arranged by size: 891 780 725 715 700 652 536 230 BsmI GAATG_Cn' Cuts at: 0 257 701 918 5229 Size: 257 444 217 4311 Fragments arranged by size: 4311 444 257 217 BsmBI CGTCTCn'nnnn_ Cuts at: 0 4020 4874 5229 Size: 4020 854 355 Fragments arranged by size: 4020 854 355 Bsp1286I G_dGCh'C Cuts at: 0 715 915 1516 1606 2013 2386 2616 3341 Size: 715 200 601 90 407 373 230 725 Cuts at: 3341 3877 4577 4982 5037 5112 5229 Size: 536 700 405 55 75 117 Fragments arranged by size: 725 715 700 601 536 407 405 373 230 200 117 90 75 55 BspEI T'CCGG_A Cuts at: 0 1137 5229 Size: 1137 4092 BspHI T'CATG_A Cuts at: 0 3179 5229 Size: 3179 2050 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1764 2566 2837 3100 4046 4146 4958 5229 Size: 1764 802 271 263 946 100 812 271 Fragments arranged by size: 1764 946 812 802 271 271 263 100 BsrBI CCG'CTC Cuts at: 0 27 1656 3813 4236 4776 5229 Size: 27 1629 2157 423 540 453 Fragments arranged by size: 2157 1629 540 453 423 27 BsrDI GCAATG_nn' Cuts at: 0 523 1940 5229 Size: 523 1417 3289 Fragments arranged by size: 3289 1417 523 BsrFI r'CCGG_y Cuts at: 0 68 1877 3996 4075 4613 4850 5229 Size: 68 1809 2119 79 538 237 379 Fragments arranged by size: 2119 1809 538 379 237 79 68 BsrGI T'GTAC_A Cuts at: 0 336 5229 Size: 336 4893 BssHII G'CGCG_C Cuts at: 0 4963 5229 Size: 4963 266 BstAPI GCAn_nnn'nTGC Cuts at: 0 823 3031 4536 5229 Size: 823 2208 1505 693 Fragments arranged by size: 2208 1505 823 693 BstEII G'GTnAC_C Cuts at: 0 2358 3933 5229 Size: 2358 1575 1296 Fragments arranged by size: 2358 1575 1296 BstXI CCAn_nnnn'nTGG Cuts at: 0 72 1273 1347 1614 2879 4008 5229 Size: 72 1201 74 267 1265 1129 1221 Fragments arranged by size: 1265 1221 1201 1129 267 74 72 BstYI r'GATC_y Cuts at: 0 810 1483 2640 2727 4565 5011 5229 Size: 810 673 1157 87 1838 446 218 Fragments arranged by size: 1838 1157 810 673 446 218 87 Bsu36I CC'TnA_GG Cuts at: 0 410 650 872 5229 Size: 410 240 222 4357 Fragments arranged by size: 4357 410 240 222 BtgI C'CryG_G Cuts at: 0 117 2513 3237 4097 4492 5229 Size: 117 2396 724 860 395 737 Fragments arranged by size: 2396 860 737 724 395 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 870 5229 Size: 870 4359 BtsI GCAGTG_nn' Cuts at: 0 2675 2703 5229 Size: 2675 28 2526 Fragments arranged by size: 2675 2526 28 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 3179 3214 5229 Size: 3179 35 2015 Fragments arranged by size: 3179 2015 35 DraI TTT'AAA Cuts at: 0 767 2133 5229 Size: 767 1366 3096 Fragments arranged by size: 3096 1366 767 DraIII CAC_nnn'GTG Cuts at: 0 4545 5229 Size: 4545 684 EaeI y'GGCC_r Cuts at: 0 12 38 991 3245 3694 3969 3998 4010 Size: 12 26 953 2254 449 275 29 12 Cuts at: 4010 4363 5229 Size: 353 866 Fragments arranged by size: 2254 953 866 449 353 275 29 26 12 12 EagI C'GGCC_G Cuts at: 0 12 5229 Size: 12 5217 EarI CTCTTCn'nnn_ Cuts at: 0 502 1021 2195 2314 2326 4645 4746 4793 Size: 502 519 1174 119 12 2319 101 47 Cuts at: 4793 4838 5229 Size: 45 391 Fragments arranged by size: 2319 1174 519 502 391 119 101 47 45 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 45 3575 4034 4116 5229 Size: 45 3530 459 82 1113 Fragments arranged by size: 3530 1113 459 82 45 EcoICRI GAG'CTC Cuts at: 0 713 3339 3875 4575 5229 Size: 713 2626 536 700 654 Fragments arranged by size: 2626 713 700 654 536 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 854 929 966 1198 1250 1805 2219 3518 Size: 854 75 37 232 52 555 414 1299 Cuts at: 3518 3617 3873 4046 4246 4342 4540 4561 4642 Size: 99 256 173 200 96 198 21 81 Cuts at: 4642 4654 4759 4907 4996 5039 5227 5229 Size: 12 105 148 89 43 188 2 Fragments arranged by size: 1299 854 555 414 256 232 200 198 188 173 148 105 99 96 89 81 75 52 43 37 21 12 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 560 731 4228 5229 Size: 560 171 3497 1001 Fragments arranged by size: 3497 1001 560 171 EcoO109I rG'GnC_Cy Cuts at: 0 1512 5108 5109 5229 Size: 1512 3596 1 120 Fragments arranged by size: 3596 1512 120 1 EcoRI G'AATT_C Cuts at: 0 1283 1768 5229 Size: 1283 485 3461 Fragments arranged by size: 3461 1283 485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1086 3799 4477 5229 Size: 1086 2713 678 752 Fragments arranged by size: 2713 1086 752 678 FspI TGC'GCA Cuts at: 0 4154 4282 4621 4807 5229 Size: 4154 128 339 186 422 Fragments arranged by size: 4154 422 339 186 128 FspAI rTGC'GCAy Cuts at: 0 4282 5229 Size: 4282 947 HaeII r_GCGC'y Cuts at: 0 4601 4737 4851 4990 5070 5229 Size: 4601 136 114 139 80 159 Fragments arranged by size: 4601 159 139 136 114 80 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1243 1275 1615 1647 1895 1927 2394 2426 Size: 1243 32 340 32 248 32 467 32 Cuts at: 2426 3663 3695 3829 3861 4078 4110 4269 4301 Size: 1237 32 134 32 217 32 159 32 Cuts at: 4301 4465 4497 5229 Size: 164 32 732 Fragments arranged by size: 1243 1237 732 467 340 248 217 164 159 134 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1953 5229 Size: 1953 3276 HindIII A'AGCT_T Cuts at: 0 836 1201 3797 5183 5229 Size: 836 365 2596 1386 46 Fragments arranged by size: 2596 1386 836 365 46 KasI G'GCGC_C Cuts at: 0 4986 5229 Size: 4986 243 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1229 1916 2059 2699 3129 3955 5010 5229 Size: 1229 687 143 640 430 826 1055 219 Fragments arranged by size: 1229 1055 826 687 640 430 219 143 MscI TGG'CCA Cuts at: 0 993 3247 3696 5229 Size: 993 2254 449 1533 Fragments arranged by size: 2254 1533 993 449 MslI CAynn'nnrTG Cuts at: 0 923 1298 1414 2335 4006 5229 Size: 923 375 116 921 1671 1223 Fragments arranged by size: 1671 1223 923 921 375 116 MspA1I CmG'CkG Cuts at: 0 296 2742 3384 4362 5026 5229 Size: 296 2446 642 978 664 203 Fragments arranged by size: 2446 978 664 642 296 203 NaeI GCC'GGC Cuts at: 0 4852 5229 Size: 4852 377 NarI GG'CG_CC Cuts at: 0 4987 5229 Size: 4987 242 NcoI C'CATG_G Cuts at: 0 117 2513 4097 5229 Size: 117 2396 1584 1132 Fragments arranged by size: 2396 1584 1132 117 NdeI CA'TA_TG Cuts at: 0 1857 3513 3719 5229 Size: 1857 1656 206 1510 Fragments arranged by size: 1857 1656 1510 206 NgoMIV G'CCGG_C Cuts at: 0 4850 5229 Size: 4850 379 NsiI A_TGCA'T Cuts at: 0 2037 5229 Size: 2037 3192 NspI r_CATG'y Cuts at: 0 292 615 1877 2178 4554 5229 Size: 292 323 1262 301 2376 675 Fragments arranged by size: 2376 1262 675 323 301 292 PflMI CCAn_nnn'nTGG Cuts at: 0 2764 3243 5229 Size: 2764 479 1986 Fragments arranged by size: 2764 1986 479 PfoI T'CCnGG_A Cuts at: 0 906 3495 4568 4658 5229 Size: 906 2589 1073 90 571 Fragments arranged by size: 2589 1073 906 571 90 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3907 3939 4627 4659 5229 Size: 3907 32 688 32 570 Fragments arranged by size: 3907 688 570 32 32 PshAI GACnn'nnGTC Cuts at: 0 1457 2075 5045 5229 Size: 1457 618 2970 184 Fragments arranged by size: 2970 1457 618 184 PsiI TTA'TAA Cuts at: 0 1810 5229 Size: 1810 3419 PspOMI G'GGCC_C Cuts at: 0 1512 5108 5229 Size: 1512 3596 121 Fragments arranged by size: 3596 1512 121 PstI C_TGCA'G Cuts at: 0 819 1403 2281 2741 3090 3111 4394 5229 Size: 819 584 878 460 349 21 1283 835 Fragments arranged by size: 1283 878 835 819 584 460 349 21 PvuI CG_AT'CG Cuts at: 0 4418 5229 Size: 4418 811 PvuII CAG'CTG Cuts at: 0 2742 3384 5026 5229 Size: 2742 642 1642 203 Fragments arranged by size: 2742 1642 642 203 RsrII CG'GwC_CG Cuts at: 0 4590 5229 Size: 4590 639 SacI G_AGCT'C Cuts at: 0 715 3341 3877 4577 5229 Size: 715 2626 536 700 652 Fragments arranged by size: 2626 715 700 652 536 SapI GCTCTTCn'nnn_ Cuts at: 0 2314 2326 4746 4838 5229 Size: 2314 12 2420 92 391 Fragments arranged by size: 2420 2314 391 92 12 ScaI AGT'ACT Cuts at: 0 192 5229 Size: 192 5037 SexAI A'CCwGG_T Cuts at: 0 2871 5229 Size: 2871 2358 SfcI C'TryA_G Cuts at: 0 806 815 1399 1829 1919 2277 2737 3086 Size: 806 9 584 430 90 358 460 349 Cuts at: 3086 3107 4390 5229 Size: 21 1283 839 Fragments arranged by size: 1283 839 806 584 460 430 358 349 90 21 9 SfoI GGC'GCC Cuts at: 0 4988 5229 Size: 4988 241 SgrAI Cr'CCGG_yG Cuts at: 0 4850 5229 Size: 4850 379 SmaI CCC'GGG Cuts at: 0 892 5229 Size: 892 4337 SmlI C'TyrA_G Cuts at: 0 203 382 2096 3808 5229 Size: 203 179 1714 1712 1421 Fragments arranged by size: 1714 1712 1421 203 179 SphI G_CATG'C Cuts at: 0 1877 5229 Size: 1877 3352 SspI AAT'ATT Cuts at: 0 777 2001 5229 Size: 777 1224 3228 Fragments arranged by size: 3228 1224 777 StuI AGG'CCT Cuts at: 0 1499 5229 Size: 1499 3730 StyI C'CwwG_G Cuts at: 0 117 561 1088 2513 3388 4097 4508 5229 Size: 117 444 527 1425 875 709 411 721 Fragments arranged by size: 1425 875 721 709 527 444 411 117 SwaI ATTT'AAAT Cuts at: 0 2133 5229 Size: 2133 3096 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 115 1101 2081 2377 3854 3921 4841 5229 Size: 115 986 980 296 1477 67 920 388 Fragments arranged by size: 1477 986 980 920 388 296 115 67 TatI w'GTAC_w Cuts at: 0 190 336 1898 4436 5229 Size: 190 146 1562 2538 793 Fragments arranged by size: 2538 1562 793 190 146 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1119 1151 2696 2728 3044 3076 4049 4081 Size: 1119 32 1545 32 316 32 973 32 Cuts at: 4081 5229 Size: 1148 Fragments arranged by size: 1545 1148 1119 973 316 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 3140 5229 Size: 3140 2089 XbaI T'CTAG_A Cuts at: 0 1186 2187 5229 Size: 1186 1001 3042 Fragments arranged by size: 3042 1186 1001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1068 4515 5229 Size: 1068 3447 714 Fragments arranged by size: 3447 1068 714 XmaI C'CCGG_G Cuts at: 0 890 5229 Size: 890 4339 XmnI GAAnn'nnTTC Cuts at: 0 639 947 3782 5229 Size: 639 308 2835 1447 Fragments arranged by size: 2835 1447 639 308 ZraI GAC'GTC Cuts at: 0 3100 5229 Size: 3100 2129 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PflMI PfoI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SapI ScaI SexAI SfcI SfoI SgrAI SmaI SmlI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AgeI AscI AseI AsiSI AvrII BmgBI BmtI BsiWI BssSI BstBI BstZ17I ClaI DrdI EcoRV FseI HpaI KpnI MfeI MluI NheI NotI NruI PacI PasI PciI PmeI PmlI PpuMI PspXI PsrI SacII SalI SanDI SbfI SfiI SgfI SnaBI SpeI SrfI XhoI