Restriction map for
pF1KB4411
(Linear) (Six-base) MAPSORT of: KIBB4411.seq Check: 1862 from: 1 to: 558
KIBB4411.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 36 558
Size: 36 522
AlwNI CAG_nnn'CTG
Cuts at: 0 154 438 558
Size: 154 284 120
Fragments arranged by size:
284 154 120
ApaI G_GGCC'C
Cuts at: 0 552 558
Size: 552 6
AvaI C'yCGr_G
Cuts at: 0 413 558
Size: 413 145
BanII G_rGCy'C
Cuts at: 0 90 552 558
Size: 90 462 6
Fragments arranged by size:
462 90 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 558
Size: 145 413
BbvCI CC'TCA_GC
Cuts at: 0 407 558
Size: 407 151
BfrBI ATG'CAT
Cuts at: 0 23 558
Size: 23 535
BglII A'GATC_T
Cuts at: 0 68 558
Size: 68 490
Bme1580I G_kGCm'C
Cuts at: 0 552 558
Size: 552 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 331 558
Size: 317 14 227
Fragments arranged by size:
317 227 14
Bpu10I CC'TnA_GC
Cuts at: 0 407 558
Size: 407 151
BsaI GGTCTCn'nnnn_
Cuts at: 0 29 558
Size: 29 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 155 185 558
Size: 155 30 373
Fragments arranged by size:
373 155 30
BseYI C'CCAG_C
Cuts at: 0 39 426 558
Size: 39 387 132
Fragments arranged by size:
387 132 39
Bsp1286I G_dGCh'C
Cuts at: 0 90 552 558
Size: 90 462 6
Fragments arranged by size:
462 90 6
BsrFI r'CCGG_y
Cuts at: 0 382 558
Size: 382 176
BstBI TT'CG_AA
Cuts at: 0 479 558
Size: 479 79
BstYI r'GATC_y
Cuts at: 0 68 558
Size: 68 490
BtsI GCAGTG_nn'
Cuts at: 0 50 84 401 492 558
Size: 50 34 317 91 66
Fragments arranged by size:
317 91 66 50 34
EaeI y'GGCC_r
Cuts at: 0 380 558
Size: 380 178
EarI CTCTTCn'nnn_
Cuts at: 0 184 558
Size: 184 374
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 331 558
Size: 317 14 227
Fragments arranged by size:
317 227 14
EcoO109I rG'GnC_Cy
Cuts at: 0 435 549 558
Size: 435 114 9
Fragments arranged by size:
435 114 9
HaeII r_GCGC'y
Cuts at: 0 427 558
Size: 427 131
MspA1I CmG'CkG
Cuts at: 0 406 519 558
Size: 406 113 39
Fragments arranged by size:
406 113 39
NaeI GCC'GGC
Cuts at: 0 384 558
Size: 384 174
NgoMIV G'CCGG_C
Cuts at: 0 382 558
Size: 382 176
NsiI A_TGCA'T
Cuts at: 0 25 558
Size: 25 533
PflMI CCAn_nnn'nTGG
Cuts at: 0 438 558
Size: 438 120
PspOMI G'GGCC_C
Cuts at: 0 548 558
Size: 548 10
PstI C_TGCA'G
Cuts at: 0 301 558
Size: 301 257
PvuII CAG'CTG
Cuts at: 0 406 519 558
Size: 406 113 39
Fragments arranged by size:
406 113 39
SfcI C'TryA_G
Cuts at: 0 297 558
Size: 297 261
SmaI CCC'GGG
Cuts at: 0 415 558
Size: 415 143
SrfI GCCC'GGGC
Cuts at: 0 415 558
Size: 415 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 311 558
Size: 311 247
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 56 88 155 187 217 249 558
Size: 56 32 67 32 30 32 309
Fragments arranged by size:
309 67 56 32 32 32 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 558
Size: 24 534
XmaI C'CCGG_G
Cuts at: 0 413 558
Size: 413 145
Enzymes that do cut:
AlfI AlwNI ApaI AvaI BanII BbsI BbvCI BfrBI
BglII Bme1580I BpmI Bpu10I BsaI BsaXI BseYI Bsp1286I
BsrFI BstBI BstYI BtsI EaeI EarI Eco57MI EcoO109I
HaeII MspA1I NaeI NgoMIV NsiI PflMI PspOMI PstI
PvuII SfcI SmaI SrfI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BcgI
BciVI BclI BglI BlpI BmgBI BmrI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NarI NcoI NdeI
NheI NotI NruI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI