(Linear) (Six-base) MAPSORT of: KIBB4415.seq  Check: 7747  from: 1  to: 390



KIBB4415.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     365     390 
   Size:        365      25
 
ApaLI G'TGCA_C

Cuts at:      0      91     390 
   Size:         91     299
 
AscI GG'CGCG_CC

Cuts at:      0     140     390 
   Size:        140     250
 
BanII G_rGCy'C

Cuts at:      0     281     390 
   Size:        281     109
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     232     364     390 
   Size:        232     132      26

  Fragments arranged by size: 

                 232     132      26
 
Bme1580I G_kGCm'C

Cuts at:      0      95     390 
   Size:         95     295
 
BmgBI CAC'GTC

Cuts at:      0      10     390 
   Size:         10     380
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     211     390 
   Size:         55     156     179

  Fragments arranged by size: 

                 179     156      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     311     390 
   Size:        311      79
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      73     390 
   Size:         73     317
 
BsaHI Gr'CG_yC

Cuts at:      0     320     390 
   Size:        320      70
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     289     390 
   Size:        289     101
 
BsiHKAI G_wGCw'C

Cuts at:      0      95     281     390 
   Size:         95     186     109

  Fragments arranged by size: 

                 186     109      95
 
Bsp1286I G_dGCh'C

Cuts at:      0      95     281     390 
   Size:         95     186     109

  Fragments arranged by size: 

                 186     109      95
 
BsrBI CCG'CTC

Cuts at:      0      53     126     390 
   Size:         53      73     264

  Fragments arranged by size: 

                 264      73      53
 
BsrDI GCAATG_nn'

Cuts at:      0     305     390 
   Size:        305      85
 
BsrFI r'CCGG_y

Cuts at:      0     144     199     390 
   Size:        144      55     191

  Fragments arranged by size: 

                 191     144      55
 
BssHII G'CGCG_C

Cuts at:      0     140     390 
   Size:        140     250
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     390 
   Size:         99     291
 
BstEII G'GTnAC_C

Cuts at:      0     300     390 
   Size:        300      90
 
BstYI r'GATC_y

Cuts at:      0     185     390 
   Size:        185     205
 
EaeI y'GGCC_r

Cuts at:      0     197     257     390 
   Size:        197      60     133

  Fragments arranged by size: 

                 197     133      60
 
EarI CTCTTCn'nnn_

Cuts at:      0      60     390 
   Size:         60     330
 
EcoICRI GAG'CTC

Cuts at:      0     279     390 
   Size:        279     111
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     211     390 
   Size:         55     156     179

  Fragments arranged by size: 

                 179     156      55
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     294     326     351     383     390 
   Size:        294      32      25      32       7

  Fragments arranged by size: 

                 294      32      32      25       7
 
MscI TGG'CCA

Cuts at:      0     259     390 
   Size:        259     131
 
MspA1I CmG'CkG

Cuts at:      0     255     390 
   Size:        255     135
 
NaeI GCC'GGC

Cuts at:      0     201     390 
   Size:        201     189
 
NgoMIV G'CCGG_C

Cuts at:      0     199     390 
   Size:        199     191
 
NruI TCG'CGA

Cuts at:      0     215     390 
   Size:        215     175
 
PfoI T'CCnGG_A

Cuts at:      0     188     390 
   Size:        188     202
 
PvuII CAG'CTG

Cuts at:      0     255     390 
   Size:        255     135
 
SacI G_AGCT'C

Cuts at:      0     281     390 
   Size:        281     109
 
SapI GCTCTTCn'nnn_

Cuts at:      0      60     390 
   Size:         60     330
 
SgrAI Cr'CCGG_yG

Cuts at:      0     144     390 
   Size:        144     246
 
StyI C'CwwG_G

Cuts at:      0     380     390 
   Size:        380      10
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     376     390 
   Size:        376      14
 

 Enzymes that do cut:

     AhdI    ApaLI     AscI    BanII     BbsI Bme1580I    BmgBI     BpmI
   Bpu10I     BsaI    BsaHI    BseRI  BsiHKAI Bsp1286I    BsrBI    BsrDI
    BsrFI   BssHII   BstAPI   BstEII    BstYI     EaeI     EarI  EcoICRI
  Eco57MI    Hin4I     MscI   MspA1I     NaeI   NgoMIV     NruI     PfoI
    PvuII     SacI     SapI    SgrAI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI     ApoI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmrI     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrGI    BssSI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI     NotI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI