(Linear) (Six-base) MAPSORT of: KIBB4445.seq  Check: 5969  from: 1  to: 447



KIBB4445.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:25  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     447 
   Size:        155     292
 
BclI T'GATC_A

Cuts at:      0     155     374     447 
   Size:        155     219      73

  Fragments arranged by size: 

                 219     155      73
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      66     447 
   Size:         66     381
 
BsaAI yAC'GTr

Cuts at:      0     324     447 
   Size:        324     123
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      34     262     265     373     447 
   Size:         34     228       3     108      74

  Fragments arranged by size: 

                 228     108      74      34       3
 
BsrGI T'GTAC_A

Cuts at:      0     426     447 
   Size:        426      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96     447 
   Size:         96     351
 
BstYI r'GATC_y

Cuts at:      0     112     254     447 
   Size:        112     142     193

  Fragments arranged by size: 

                 193     142     112
 
ClaI AT'CG_AT

Cuts at:      0     392     447 
   Size:        392      55
 
EaeI y'GGCC_r

Cuts at:      0     218     402     447 
   Size:        218     184      45

  Fragments arranged by size: 

                 218     184      45
 
EarI CTCTTCn'nnn_

Cuts at:      0     413     447 
   Size:        413      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155     447 
   Size:        155     292
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      63     447 
   Size:         63     384
 
HaeII r_GCGC'y

Cuts at:      0     308     447 
   Size:        308     139
 
MscI TGG'CCA

Cuts at:      0     220     404     447 
   Size:        220     184      43

  Fragments arranged by size: 

                 220     184      43
 
MspA1I CmG'CkG

Cuts at:      0      12     447 
   Size:         12     435
 
PasI CC'CwG_GG

Cuts at:      0     117     447 
   Size:        117     330
 
PfoI T'CCnGG_A

Cuts at:      0     197     447 
   Size:        197     250
 
PstI C_TGCA'G

Cuts at:      0     149     447 
   Size:        149     298
 
PvuII CAG'CTG

Cuts at:      0      12     447 
   Size:         12     435
 
SfcI C'TryA_G

Cuts at:      0     145     447 
   Size:        145     302
 
StuI AGG'CCT

Cuts at:      0      46     447 
   Size:         46     401
 
StyI C'CwwG_G

Cuts at:      0      89     447 
   Size:         89     358
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     367     447 
   Size:        367      80
 
TatI w'GTAC_w

Cuts at:      0     426     447 
   Size:        426      21
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     447 
   Size:         93     354
 

 Enzymes that do cut:

     AcuI     BclI     BplI    BsaAI    BseRI    BsrGI    BstXI    BstYI
     ClaI     EaeI     EarI  Eco57MI     FalI    HaeII     MscI   MspA1I
     PasI     PfoI     PstI    PvuII     SfcI     StuI     StyI    TaqII
     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI