Restriction map for
pF1KB4450
(Linear) (Six-base) MAPSORT of: KIBB4450.seq Check: 1972 from: 1 to: 1140
KIBB4450.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 892 977 1140
Size: 892 85 163
Fragments arranged by size:
892 163 85
AflIII A'CryG_T
Cuts at: 0 104 263 335 1140
Size: 104 159 72 805
Fragments arranged by size:
805 159 104 72
AhdI GACnn_n'nnGTC
Cuts at: 0 45 1140
Size: 45 1095
AleI CACnn'nnGTG
Cuts at: 0 532 619 1140
Size: 532 87 521
Fragments arranged by size:
532 521 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 844 982 1140
Size: 844 138 158
Fragments arranged by size:
844 158 138
ApaI G_GGCC'C
Cuts at: 0 87 1061 1140
Size: 87 974 79
Fragments arranged by size:
974 87 79
AvaI C'yCGr_G
Cuts at: 0 78 1140
Size: 78 1062
BanI G'GyrC_C
Cuts at: 0 124 633 728 997 1140
Size: 124 509 95 269 143
Fragments arranged by size:
509 269 143 124 95
BanII G_rGCy'C
Cuts at: 0 87 577 1061 1140
Size: 87 490 484 79
Fragments arranged by size:
490 484 87 79
BbeI G_GCGC'C
Cuts at: 0 732 1140
Size: 732 408
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 342 808 1140
Size: 105 237 466 332
Fragments arranged by size:
466 332 237 105
BbvCI CC'TCA_GC
Cuts at: 0 367 418 1140
Size: 367 51 722
Fragments arranged by size:
722 367 51
BglI GCCn_nnn'nGGC
Cuts at: 0 362 478 1140
Size: 362 116 662
Fragments arranged by size:
662 362 116
BlpI GC'TnA_GC
Cuts at: 0 186 310 739 1140
Size: 186 124 429 401
Fragments arranged by size:
429 401 186 124
Bme1580I G_kGCm'C
Cuts at: 0 87 127 638 1049 1061 1140
Size: 87 40 511 411 12 79
Fragments arranged by size:
511 411 87 79 40 12
BmtI G_CTAG'C
Cuts at: 0 209 1140
Size: 209 931
Bpu10I CC'TnA_GC
Cuts at: 0 367 418 1140
Size: 367 51 722
Fragments arranged by size:
722 367 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 612 1117 1140
Size: 612 505 23
Fragments arranged by size:
612 505 23
BsaAI yAC'GTr
Cuts at: 0 266 1140
Size: 266 874
BsaHI Gr'CG_yC
Cuts at: 0 729 1140
Size: 729 411
BseYI C'CCAG_C
Cuts at: 0 816 1140
Size: 816 324
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 814 864 942 1140
Size: 556 258 50 78 198
Fragments arranged by size:
556 258 198 78 50
BsiEI CG_ry'CG
Cuts at: 0 378 1140
Size: 378 762
BsiHKAI G_wGCw'C
Cuts at: 0 149 577 1140
Size: 149 428 563
Fragments arranged by size:
563 428 149
Bsp1286I G_dGCh'C
Cuts at: 0 87 127 149 577 638 1049 1061 1140
Size: 87 40 22 428 61 411 12 79
Fragments arranged by size:
428 411 87 79 61 40 22 12
BspHI T'CATG_A
Cuts at: 0 860 1140
Size: 860 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 892 977 1140
Size: 892 85 163
Fragments arranged by size:
892 163 85
BsrDI GCAATG_nn'
Cuts at: 0 200 1140
Size: 200 940
BsrFI r'CCGG_y
Cuts at: 0 361 731 1140
Size: 361 370 409
Fragments arranged by size:
409 370 361
BsrGI T'GTAC_A
Cuts at: 0 809 1140
Size: 809 331
BssSI C'ACGA_G
Cuts at: 0 1096 1140
Size: 1096 44
BstEII G'GTnAC_C
Cuts at: 0 762 1140
Size: 762 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 621 1140
Size: 621 519
BtgI C'CryG_G
Cuts at: 0 128 440 446 557 620 1140
Size: 128 312 6 111 63 520
Fragments arranged by size:
520 312 128 111 63 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 654 666 1140
Size: 654 12 474
Fragments arranged by size:
654 474 12
BtsI GCAGTG_nn'
Cuts at: 0 544 1020 1140
Size: 544 476 120
Fragments arranged by size:
544 476 120
DraIII CAC_nnn'GTG
Cuts at: 0 798 1140
Size: 798 342
EaeI y'GGCC_r
Cuts at: 0 386 404 443 467 479 563 687 978
Size: 386 18 39 24 12 84 124 291
Cuts at: 978 1140
Size: 162
Fragments arranged by size:
386 291 162 124 84 39 24 18
12
EarI CTCTTCn'nnn_
Cuts at: 0 172 859 1044 1140
Size: 172 687 185 96
Fragments arranged by size:
687 185 172 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 937 1140
Size: 937 203
EcoICRI GAG'CTC
Cuts at: 0 575 1140
Size: 575 565
EcoO109I rG'GnC_Cy
Cuts at: 0 83 84 293 714 1057 1140
Size: 83 1 209 421 343 83
Fragments arranged by size:
421 343 209 83 83 1
EcoRV GAT'ATC
Cuts at: 0 195 1140
Size: 195 945
FspI TGC'GCA
Cuts at: 0 993 1140
Size: 993 147
HaeII r_GCGC'y
Cuts at: 0 186 732 1140
Size: 186 546 408
Fragments arranged by size:
546 408 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 58 63 90 1140
Size: 31 27 5 27 1050
Fragments arranged by size:
1050 31 27 27 5
HincII GTy'rAC
Cuts at: 0 333 900 1137 1140
Size: 333 567 237 3
Fragments arranged by size:
567 333 237 3
KasI G'GCGC_C
Cuts at: 0 728 1140
Size: 728 412
MscI TGG'CCA
Cuts at: 0 388 406 445 481 565 689 980 1140
Size: 388 18 39 36 84 124 291 160
Fragments arranged by size:
388 291 160 124 84 39 36 18
MslI CAynn'nnrTG
Cuts at: 0 103 532 619 646 748 751 964 1140
Size: 103 429 87 27 102 3 213 176
Fragments arranged by size:
429 213 176 103 102 87 27 3
MspA1I CmG'CkG
Cuts at: 0 190 434 905 1140
Size: 190 244 471 235
Fragments arranged by size:
471 244 235 190
NaeI GCC'GGC
Cuts at: 0 733 1140
Size: 733 407
NarI GG'CG_CC
Cuts at: 0 729 1140
Size: 729 411
NcoI C'CATG_G
Cuts at: 0 557 1140
Size: 557 583
NgoMIV G'CCGG_C
Cuts at: 0 731 1140
Size: 731 409
NheI G'CTAG_C
Cuts at: 0 205 1140
Size: 205 935
NspI r_CATG'y
Cuts at: 0 108 339 816 1140
Size: 108 231 477 324
Fragments arranged by size:
477 324 231 108
PciI A'CATG_T
Cuts at: 0 104 335 1140
Size: 104 231 805
Fragments arranged by size:
805 231 104
PpuMI rG'GwC_Cy
Cuts at: 0 293 1140
Size: 293 847
PspOMI G'GGCC_C
Cuts at: 0 83 1057 1140
Size: 83 974 83
Fragments arranged by size:
974 83 83
PvuII CAG'CTG
Cuts at: 0 190 905 1140
Size: 190 715 235
Fragments arranged by size:
715 235 190
SacI G_AGCT'C
Cuts at: 0 577 1140
Size: 577 563
SexAI A'CCwGG_T
Cuts at: 0 794 1140
Size: 794 346
SfcI C'TryA_G
Cuts at: 0 1113 1140
Size: 1113 27
SfoI GGC'GCC
Cuts at: 0 730 1140
Size: 730 410
StuI AGG'CCT
Cuts at: 0 955 1140
Size: 955 185
StyI C'CwwG_G
Cuts at: 0 557 600 1140
Size: 557 43 540
Fragments arranged by size:
557 540 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 1140
Size: 450 690
TatI w'GTAC_w
Cuts at: 0 59 809 1140
Size: 59 750 331
Fragments arranged by size:
750 331 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1087 1119 1140
Size: 1087 32 21
Fragments arranged by size:
1087 32 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 105 489 513 682 1012 1140
Size: 105 384 24 169 330 128
Fragments arranged by size:
384 330 169 128 105 24
XmnI GAAnn'nnTTC
Cuts at: 0 868 1140
Size: 868 272
Enzymes that do cut:
AarI AflIII AhdI AleI AlfI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BmtI
Bpu10I BsaI BsaAI BsaHI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BssSI BstEII
BstXI BtgI BtgZI BtsI DraIII EaeI EarI EciI
EcoICRI EcoO109I EcoRV FspI HaeII Hin4I HincII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NheI
NspI PciI PpuMI PspOMI PvuII SacI SexAI SfcI
SfoI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BmgBI
BmrI BplI BpmI BpuEI BsaBI BsaWI BsaXI BseRI
BsiWI BsmI BsmBI BspEI BsrBI BssHII BstAPI BstBI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI
Eco57MI EcoNI EcoRI FalI FseI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NdeI NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI