Restriction map for
pF1KB4456
(Linear) (Six-base) MAPSORT of: KIBB4456.seq Check: 7758 from: 1 to: 2376
KIBB4456.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 273 688 1021 1257 1753 2376
Size: 273 415 333 236 496 623
Fragments arranged by size:
623 496 415 333 273 236
AatII G_ACGT'C
Cuts at: 0 2198 2376
Size: 2198 178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 586 2072 2376
Size: 345 241 1486 304
Fragments arranged by size:
1486 345 304 241
AfeI AGC'GCT
Cuts at: 0 742 2376
Size: 742 1634
AflIII A'CryG_T
Cuts at: 0 1286 2376
Size: 1286 1090
AhdI GACnn_n'nnGTC
Cuts at: 0 1569 1785 2376
Size: 1569 216 591
Fragments arranged by size:
1569 591 216
AleI CACnn'nnGTG
Cuts at: 0 362 433 2376
Size: 362 71 1943
Fragments arranged by size:
1943 362 71
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 631 2376
Size: 631 1745
AlwNI CAG_nnn'CTG
Cuts at: 0 279 288 535 2376
Size: 279 9 247 1841
Fragments arranged by size:
1841 279 247 9
ApaI G_GGCC'C
Cuts at: 0 121 212 272 450 898 1348 1413 1652
Size: 121 91 60 178 448 450 65 239
Cuts at: 1652 2376
Size: 724
Fragments arranged by size:
724 450 448 239 178 121 91 65
60
ApaLI G'TGCA_C
Cuts at: 0 1231 1927 2376
Size: 1231 696 449
Fragments arranged by size:
1231 696 449
AvaI C'yCGr_G
Cuts at: 0 114 190 390 466 1372 1406 1719 1756
Size: 114 76 200 76 906 34 313 37
Cuts at: 1756 1808 1919 2245 2376
Size: 52 111 326 131
Fragments arranged by size:
906 326 313 200 131 114 111 76
76 52 37 34
BanI G'GyrC_C
Cuts at: 0 4 55 243 654 1630 2224 2376
Size: 4 51 188 411 976 594 152
Fragments arranged by size:
976 594 411 188 152 51 4
BanII G_rGCy'C
Cuts at: 0 121 128 212 272 450 898 1222 1300
Size: 121 7 84 60 178 448 324 78
Cuts at: 1300 1348 1373 1413 1652 2009 2040 2342 2376
Size: 48 25 40 239 357 31 302 34
Fragments arranged by size:
448 357 324 302 239 178 121 84
78 60 48 40 34 31 25 7
BbeI G_GCGC'C
Cuts at: 0 8 59 1634 2228 2376
Size: 8 51 1575 594 148
Fragments arranged by size:
1575 594 148 51 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 410 1957 2376
Size: 141 269 1547 419
Fragments arranged by size:
1547 419 269 141
BbvCI CC'TCA_GC
Cuts at: 0 422 1129 2376
Size: 422 707 1247
Fragments arranged by size:
1247 707 422
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1363 1397 2376
Size: 1363 34 979
Fragments arranged by size:
1363 979 34
BclI T'GATC_A
Cuts at: 0 2106 2376
Size: 2106 270
BglI GCCn_nnn'nGGC
Cuts at: 0 49 276 499 902 1022 2376
Size: 49 227 223 403 120 1354
Fragments arranged by size:
1354 403 227 223 120 49
BglII A'GATC_T
Cuts at: 0 1238 1989 2376
Size: 1238 751 387
Fragments arranged by size:
1238 751 387
BlpI GC'TnA_GC
Cuts at: 0 175 2376
Size: 175 2201
Bme1580I G_kGCm'C
Cuts at: 0 121 212 248 272 450 659 898 1235
Size: 121 91 36 24 178 209 239 337
Cuts at: 1235 1348 1413 1652 1931 2376
Size: 113 65 239 279 445
Fragments arranged by size:
445 337 279 239 239 209 178 121
113 91 65 36 24
BmgBI CAC'GTC
Cuts at: 0 732 810 1926 2349 2376
Size: 732 78 1116 423 27
Fragments arranged by size:
1116 732 423 78 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 987 2257 2376
Size: 987 1270 119
Fragments arranged by size:
1270 987 119
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 1297 2254 2376
Size: 757 540 957 122
Fragments arranged by size:
957 757 540 122
Bpu10I CC'TnA_GC
Cuts at: 0 422 1129 2376
Size: 422 707 1247
Fragments arranged by size:
1247 707 422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 910 1240 2376
Size: 847 63 330 1136
Fragments arranged by size:
1136 847 330 63
BsaAI yAC'GTr
Cuts at: 0 870 1659 2376
Size: 870 789 717
Fragments arranged by size:
870 789 717
BsaBI GATnn'nnATC
Cuts at: 0 1897 2376
Size: 1897 479
BsaHI Gr'CG_yC
Cuts at: 0 5 56 998 1631 2195 2225 2376
Size: 5 51 942 633 564 30 151
Fragments arranged by size:
942 633 564 151 51 30 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 292 322 366 396 682 712 1225 1255
Size: 292 30 44 30 286 30 513 30
Cuts at: 1255 1813 1843 2376
Size: 558 30 533
Fragments arranged by size:
558 533 513 292 286 44 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 1093 1096 1774 2048 2266 2376
Size: 51 1042 3 678 274 218 110
Fragments arranged by size:
1042 678 274 218 110 51 3
BseYI C'CCAG_C
Cuts at: 0 272 528 798 1626 1999 2264 2327 2376
Size: 272 256 270 828 373 265 63 49
Fragments arranged by size:
828 373 272 270 265 256 63 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1620 2071 2376
Size: 1620 451 305
Fragments arranged by size:
1620 451 305
BsiEI CG_ry'CG
Cuts at: 0 503 2376
Size: 503 1873
BsiHKAI G_wGCw'C
Cuts at: 0 596 926 1235 1300 1373 1931 2040 2376
Size: 596 330 309 65 73 558 109 336
Fragments arranged by size:
596 558 336 330 309 109 73 65
BsmI GAATG_Cn'
Cuts at: 0 297 2376
Size: 297 2079
Bsp1286I G_dGCh'C
Cuts at: 0 121 128 212 248 272 450 596 659
Size: 121 7 84 36 24 178 146 63
Cuts at: 659 898 926 1222 1235 1300 1348 1373 1413
Size: 239 28 296 13 65 48 25 40
Cuts at: 1413 1652 1931 2009 2040 2342 2376
Size: 239 279 78 31 302 34
Fragments arranged by size:
302 296 279 239 239 178 146 121
84 78 65 63 48 40 36 34
31 28 25 24 13 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 273 688 1021 1257 1631 1697 1753 2316
Size: 273 415 333 236 374 66 56 563
Cuts at: 2316 2376
Size: 60
Fragments arranged by size:
563 415 374 333 273 236 66 60
56
BsrBI CCG'CTC
Cuts at: 0 103 471 1254 2376
Size: 103 368 783 1122
Fragments arranged by size:
1122 783 368 103
BsrDI GCAATG_nn'
Cuts at: 0 1872 2376
Size: 1872 504
BsrFI r'CCGG_y
Cuts at: 0 52 326 498 752 777 2376
Size: 52 274 172 254 25 1599
Fragments arranged by size:
1599 274 254 172 52 25
BsrGI T'GTAC_A
Cuts at: 0 830 2100 2376
Size: 830 1270 276
Fragments arranged by size:
1270 830 276
BssSI C'ACGA_G
Cuts at: 0 301 2376
Size: 301 2075
BstEII G'GTnAC_C
Cuts at: 0 1684 1875 2376
Size: 1684 191 501
Fragments arranged by size:
1684 501 191
BstYI r'GATC_y
Cuts at: 0 1238 1989 2376
Size: 1238 751 387
Fragments arranged by size:
1238 751 387
Bsu36I CC'TnA_GG
Cuts at: 0 1335 2278 2376
Size: 1335 943 98
Fragments arranged by size:
1335 943 98
BtgI C'CryG_G
Cuts at: 0 38 203 585 666 2376
Size: 38 165 382 81 1710
Fragments arranged by size:
1710 382 165 81 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1305 2376
Size: 1305 1071
BtsI GCAGTG_nn'
Cuts at: 0 634 819 1116 2338 2376
Size: 634 185 297 1222 38
Fragments arranged by size:
1222 634 297 185 38
EaeI y'GGCC_r
Cuts at: 0 35 41 50 129 491 500 779 1355
Size: 35 6 9 79 362 9 279 576
Cuts at: 1355 1452 1676 2251 2376
Size: 97 224 575 125
Fragments arranged by size:
576 575 362 279 224 125 97 79
35 9 9 6
EagI C'GGCC_G
Cuts at: 0 500 2376
Size: 500 1876
EarI CTCTTCn'nnn_
Cuts at: 0 2045 2376
Size: 2045 331
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1483 2376
Size: 1483 893
EcoICRI GAG'CTC
Cuts at: 0 1298 1371 2038 2376
Size: 1298 73 667 338
Fragments arranged by size:
1298 667 338 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 586 757 1297 2072 2254 2376
Size: 345 241 171 540 775 182 122
Fragments arranged by size:
775 540 345 241 182 171 122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1438 2140 2376
Size: 1438 702 236
Fragments arranged by size:
1438 702 236
EcoO109I rG'GnC_Cy
Cuts at: 0 208 268 269 314 446 447 1344 1345
Size: 208 60 1 45 132 1 897 1
Cuts at: 1345 1554 1649 1779 1851 1975 2170 2376
Size: 209 95 130 72 124 195 206
Fragments arranged by size:
897 209 208 206 195 132 130 124
95 72 60 45 1 1 1
FspI TGC'GCA
Cuts at: 0 1195 1318 2376
Size: 1195 123 1058
Fragments arranged by size:
1195 1058 123
FspAI rTGC'GCAy
Cuts at: 0 1318 2376
Size: 1318 1058
HaeII r_GCGC'y
Cuts at: 0 8 59 744 941 1634 2228 2376
Size: 8 51 685 197 693 594 148
Fragments arranged by size:
693 685 594 197 148 51 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 366 370 398 402 1555 1587 1771 1803
Size: 366 4 28 4 1153 32 184 32
Cuts at: 1803 2376
Size: 573
Fragments arranged by size:
1153 573 366 184 32 32 28 4
4
KasI G'GCGC_C
Cuts at: 0 4 55 1630 2224 2376
Size: 4 51 1575 594 152
Fragments arranged by size:
1575 594 152 51 4
MluI A'CGCG_T
Cuts at: 0 1286 2376
Size: 1286 1090
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1307 1325 1541 2376
Size: 1307 18 216 835
Fragments arranged by size:
1307 835 216 18
MscI TGG'CCA
Cuts at: 0 43 1357 2253 2376
Size: 43 1314 896 123
Fragments arranged by size:
1314 896 123 43
MslI CAynn'nnrTG
Cuts at: 0 362 433 1162 2027 2108 2376
Size: 362 71 729 865 81 268
Fragments arranged by size:
865 729 362 268 81 71
MspA1I CmG'CkG
Cuts at: 0 158 276 323 333 524 535 587 668
Size: 158 118 47 10 191 11 52 81
Cuts at: 668 2161 2376
Size: 1493 215
Fragments arranged by size:
1493 215 191 158 118 81 52 47
11 10
NaeI GCC'GGC
Cuts at: 0 54 328 500 779 2376
Size: 54 274 172 279 1597
Fragments arranged by size:
1597 279 274 172 54
NarI GG'CG_CC
Cuts at: 0 5 56 1631 2225 2376
Size: 5 51 1575 594 151
Fragments arranged by size:
1575 594 151 51 5
NcoI C'CATG_G
Cuts at: 0 203 2376
Size: 203 2173
NgoMIV G'CCGG_C
Cuts at: 0 52 326 498 777 2376
Size: 52 274 172 279 1599
Fragments arranged by size:
1599 279 274 172 52
NspI r_CATG'y
Cuts at: 0 2129 2376
Size: 2129 247
PasI CC'CwG_GG
Cuts at: 0 1422 2376
Size: 1422 954
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 535 560 2068 2376
Size: 218 317 25 1508 308
Fragments arranged by size:
1508 317 308 218 25
PmlI CAC'GTG
Cuts at: 0 870 2376
Size: 870 1506
PpuMI rG'GwC_Cy
Cuts at: 0 1554 1779 1851 1975 2170 2376
Size: 1554 225 72 124 195 206
Fragments arranged by size:
1554 225 206 195 124 72
PspOMI G'GGCC_C
Cuts at: 0 117 208 268 446 894 1344 1409 1648
Size: 117 91 60 178 448 450 65 239
Cuts at: 1648 2376
Size: 728
Fragments arranged by size:
728 450 448 239 178 117 91 65
60
PspXI vC'TCGA_Gb
Cuts at: 0 1372 2376
Size: 1372 1004
PstI C_TGCA'G
Cuts at: 0 284 767 2376
Size: 284 483 1609
Fragments arranged by size:
1609 483 284
PvuII CAG'CTG
Cuts at: 0 323 2161 2376
Size: 323 1838 215
Fragments arranged by size:
1838 323 215
RsrII CG'GwC_CG
Cuts at: 0 663 2376
Size: 663 1713
SacI G_AGCT'C
Cuts at: 0 1300 1373 2040 2376
Size: 1300 73 667 336
Fragments arranged by size:
1300 667 336 73
SacII CC_GC'GG
Cuts at: 0 588 669 2376
Size: 588 81 1707
Fragments arranged by size:
1707 588 81
SapI GCTCTTCn'nnn_
Cuts at: 0 2045 2376
Size: 2045 331
SbfI CC_TGCA'GG
Cuts at: 0 767 2376
Size: 767 1609
ScaI AGT'ACT
Cuts at: 0 1822 2240 2376
Size: 1822 418 136
Fragments arranged by size:
1822 418 136
SexAI A'CCwGG_T
Cuts at: 0 1565 1781 1799 1961 2376
Size: 1565 216 18 162 415
Fragments arranged by size:
1565 415 216 162 18
SfcI C'TryA_G
Cuts at: 0 280 318 763 2376
Size: 280 38 445 1613
Fragments arranged by size:
1613 445 280 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 49 499 2376
Size: 49 450 1877
Fragments arranged by size:
1877 450 49
SfoI GGC'GCC
Cuts at: 0 6 57 1632 2226 2376
Size: 6 51 1575 594 150
Fragments arranged by size:
1575 594 150 51 6
SmaI CCC'GGG
Cuts at: 0 116 192 1721 1810 1921 2247 2376
Size: 116 76 1529 89 111 326 129
Fragments arranged by size:
1529 326 129 116 111 89 76
SmlI C'TyrA_G
Cuts at: 0 862 925 1255 1372 2376
Size: 862 63 330 117 1004
Fragments arranged by size:
1004 862 330 117 63
SphI G_CATG'C
Cuts at: 0 2129 2376
Size: 2129 247
StuI AGG'CCT
Cuts at: 0 1214 2376
Size: 1214 1162
StyI C'CwwG_G
Cuts at: 0 71 203 1434 1679 2376
Size: 71 132 1231 245 697
Fragments arranged by size:
1231 697 245 132 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1894 2341 2376
Size: 1894 447 35
Fragments arranged by size:
1894 447 35
TatI w'GTAC_w
Cuts at: 0 830 1007 1820 2100 2238 2376
Size: 830 177 813 280 138 138
Fragments arranged by size:
830 813 280 177 138 138
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 292 324 1225 1257 2376
Size: 292 32 901 32 1119
Fragments arranged by size:
1119 901 292 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2180 2376
Size: 2180 196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1212 1308 2376
Size: 1212 96 1068
Fragments arranged by size:
1212 1068 96
XhoI C'TCGA_G
Cuts at: 0 1372 2376
Size: 1372 1004
XmaI C'CCGG_G
Cuts at: 0 114 190 1719 1808 1919 2245 2376
Size: 114 76 1529 89 111 326 131
Fragments arranged by size:
1529 326 131 114 111 89 76
ZraI GAC'GTC
Cuts at: 0 2196 2376
Size: 2196 180
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstEII BstYI Bsu36I
BtgI BtgZI BtsI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI FspAI HaeII Hin4I KasI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PmlI PpuMI PspOMI PspXI
PstI PvuII RsrII SacI SacII SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AloI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BfrBI BmrI
BmtI BsaI BsaWI BsiWI BsmBI BspEI BspHI BssHII
BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI DraIII
DrdI EcoRI EcoRV FalI FseI HincII HindIII HpaI
KpnI MfeI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PpiI PshAI PsiI PsrI PvuI
SalI SanDI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XmnI