Restriction map for
pF1KB4458
(Linear) (Six-base) MAPSORT of: KIBB4458.seq Check: 6526 from: 1 to: 3630
KIBB4458.orf
With 182 enzymes: SgfI *
March 2, 2009 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 796 877 1717 3630
Size: 796 81 840 1913
Fragments arranged by size:
1913 840 796 81
AccI GT'mk_AC
Cuts at: 0 2828 3630
Size: 2828 802
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 3054 3630
Size: 415 2639 576
Fragments arranged by size:
2639 576 415
AfeI AGC'GCT
Cuts at: 0 29 2929 3630
Size: 29 2900 701
Fragments arranged by size:
2900 701 29
AflIII A'CryG_T
Cuts at: 0 524 2318 3630
Size: 524 1794 1312
Fragments arranged by size:
1794 1312 524
AhdI GACnn_n'nnGTC
Cuts at: 0 1451 3630
Size: 1451 2179
AleI CACnn'nnGTG
Cuts at: 0 871 2681 3394 3630
Size: 871 1810 713 236
Fragments arranged by size:
1810 871 713 236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1321 1353 3630
Size: 1321 32 2277
Fragments arranged by size:
2277 1321 32
AlwNI CAG_nnn'CTG
Cuts at: 0 627 689 1173 2439 3268 3342 3630
Size: 627 62 484 1266 829 74 288
Fragments arranged by size:
1266 829 627 484 288 74 62
ApaI G_GGCC'C
Cuts at: 0 1552 1959 3474 3630
Size: 1552 407 1515 156
Fragments arranged by size:
1552 1515 407 156
ApaLI G'TGCA_C
Cuts at: 0 2500 3630
Size: 2500 1130
ApoI r'AATT_y
Cuts at: 0 189 293 342 414 757 1030 1401 2131
Size: 189 104 49 72 343 273 371 730
Cuts at: 2131 2216 2899 3616 3630
Size: 85 683 717 14
Fragments arranged by size:
730 717 683 371 343 273 189 104
85 72 49 14
AvaI C'yCGr_G
Cuts at: 0 728 841 1534 2915 3406 3630
Size: 728 113 693 1381 491 224
Fragments arranged by size:
1381 728 693 491 224 113
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1719 1752 3630
Size: 1719 33 1878
Fragments arranged by size:
1878 1719 33
BamHI G'GATC_C
Cuts at: 0 1161 2192 2731 3630
Size: 1161 1031 539 899
Fragments arranged by size:
1161 1031 899 539
BanI G'GyrC_C
Cuts at: 0 436 862 2009 3223 3630
Size: 436 426 1147 1214 407
Fragments arranged by size:
1214 1147 436 426 407
BanII G_rGCy'C
Cuts at: 0 72 1552 1559 1959 2158 3109 3474 3568
Size: 72 1480 7 400 199 951 365 94
Cuts at: 3568 3630
Size: 62
Fragments arranged by size:
1480 951 400 365 199 94 72 62
7
BbeI G_GCGC'C
Cuts at: 0 440 2013 3227 3630
Size: 440 1573 1214 403
Fragments arranged by size:
1573 1214 440 403
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 3020 3191 3630
Size: 965 2055 171 439
Fragments arranged by size:
2055 965 439 171
BbvCI CC'TCA_GC
Cuts at: 0 2801 3630
Size: 2801 829
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3442 3476 3630
Size: 3442 34 154
Fragments arranged by size:
3442 154 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1747 3630
Size: 1747 1883
BclI T'GATC_A
Cuts at: 0 2834 2891 3630
Size: 2834 57 739
Fragments arranged by size:
2834 739 57
BfrBI ATG'CAT
Cuts at: 0 2961 3630
Size: 2961 669
BglI GCCn_nnn'nGGC
Cuts at: 0 934 2333 3469 3630
Size: 934 1399 1136 161
Fragments arranged by size:
1399 1136 934 161
BglII A'GATC_T
Cuts at: 0 2765 3630
Size: 2765 865
Bme1580I G_kGCm'C
Cuts at: 0 127 648 1552 1749 1959 2504 3000 3474
Size: 127 521 904 197 210 545 496 474
Cuts at: 3474 3630
Size: 156
Fragments arranged by size:
904 545 521 496 474 210 197 156
127
BmgBI CAC'GTC
Cuts at: 0 3027 3086 3630
Size: 3027 59 544
Fragments arranged by size:
3027 544 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 677 1952 2075 2427 3256 3477 3630
Size: 677 1275 123 352 829 221 153
Fragments arranged by size:
1275 829 677 352 221 153 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 86 2787 2812 3630
Size: 86 2701 25 818
Fragments arranged by size:
2701 818 86 25
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 1645 2712 2800 3630
Size: 94 42 1509 1067 88 830
Fragments arranged by size:
1509 1067 830 94 88 42
Bpu10I CC'TnA_GC
Cuts at: 0 354 2801 3103 3630
Size: 354 2447 302 527
Fragments arranged by size:
2447 527 354 302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1342 2130 3630
Size: 1342 788 1500
Fragments arranged by size:
1500 1342 788
BsaI GGTCTCn'nnnn_
Cuts at: 0 2912 3356 3630
Size: 2912 444 274
Fragments arranged by size:
2912 444 274
BsaAI yAC'GTr
Cuts at: 0 776 821 2292 2319 3630
Size: 776 45 1471 27 1311
Fragments arranged by size:
1471 1311 776 45 27
BsaHI Gr'CG_yC
Cuts at: 0 437 965 1835 2010 3224 3630
Size: 437 528 870 175 1214 406
Fragments arranged by size:
1214 870 528 437 406 175
BsaWI w'CCGG_w
Cuts at: 0 388 1450 2158 3630
Size: 388 1062 708 1472
Fragments arranged by size:
1472 1062 708 388
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1030 1060 1072 1102 1134 1164 2609 2639
Size: 1030 30 12 30 32 30 1445 30
Cuts at: 2639 3630
Size: 991
Fragments arranged by size:
1445 1030 991 32 30 30 30 30
12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 625 1142 1145 1951 2760 3630
Size: 625 517 3 806 809 870
Fragments arranged by size:
870 809 806 625 517 3
BseYI C'CCAG_C
Cuts at: 0 596 647 1544 2151 2331 2583 2924 3355
Size: 596 51 897 607 180 252 341 431
Cuts at: 3355 3630
Size: 275
Fragments arranged by size:
897 607 596 431 341 275 252 180
51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 623 702 1068 1695 1854 2476 3334
Size: 229 394 79 366 627 159 622 858
Cuts at: 3334 3630
Size: 296
Fragments arranged by size:
858 627 622 394 366 296 229 159
79
BsiEI CG_ry'CG
Cuts at: 0 20 3630
Size: 20 3610
BsiHKAI G_wGCw'C
Cuts at: 0 658 1531 2504 3109 3630
Size: 658 873 973 605 521
Fragments arranged by size:
973 873 658 605 521
BsmI GAATG_Cn'
Cuts at: 0 1606 2963 3630
Size: 1606 1357 667
Fragments arranged by size:
1606 1357 667
BsmBI CGTCTCn'nnnn_
Cuts at: 0 758 1575 3630
Size: 758 817 2055
Fragments arranged by size:
2055 817 758
Bsp1286I G_dGCh'C
Cuts at: 0 72 127 648 658 1531 1552 1559 1749
Size: 72 55 521 10 873 21 7 190
Cuts at: 1749 1959 2158 2504 3000 3109 3474 3568 3630
Size: 210 199 346 496 109 365 94 62
Fragments arranged by size:
873 521 496 365 346 210 199 190
109 94 72 62 55 21 10 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 796 877 1717 1789 2447 3630
Size: 317 479 81 840 72 658 1183
Fragments arranged by size:
1183 840 658 479 317 81 72
BsrDI GCAATG_nn'
Cuts at: 0 269 3630
Size: 269 3361
BsrFI r'CCGG_y
Cuts at: 0 1116 1837 3630
Size: 1116 721 1793
Fragments arranged by size:
1793 1116 721
BssSI C'ACGA_G
Cuts at: 0 99 915 3630
Size: 99 816 2715
Fragments arranged by size:
2715 816 99
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1601 1670 2376 3630
Size: 1601 69 706 1254
Fragments arranged by size:
1601 1254 706 69
BstEII G'GTnAC_C
Cuts at: 0 2703 3630
Size: 2703 927
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1947 2583 3630
Size: 1947 636 1047
Fragments arranged by size:
1947 1047 636
BstYI r'GATC_y
Cuts at: 0 1161 1925 2114 2192 2731 2765 3630
Size: 1161 764 189 78 539 34 865
Fragments arranged by size:
1161 865 764 539 189 78 34
Bsu36I CC'TnA_GG
Cuts at: 0 1691 3592 3630
Size: 1691 1901 38
Fragments arranged by size:
1901 1691 38
BtgI C'CryG_G
Cuts at: 0 669 3137 3630
Size: 669 2468 493
Fragments arranged by size:
2468 669 493
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1920 3630
Size: 1920 1710
BtsI GCAGTG_nn'
Cuts at: 0 513 658 1767 3389 3401 3618 3630
Size: 513 145 1109 1622 12 217 12
Fragments arranged by size:
1622 1109 513 217 145 12 12
ClaI AT'CG_AT
Cuts at: 0 2822 3630
Size: 2822 808
DraI TTT'AAA
Cuts at: 0 1035 3630
Size: 1035 2595
DraIII CAC_nnn'GTG
Cuts at: 0 2083 3630
Size: 2083 1547
DrdI GACnn_nn'nnGTC
Cuts at: 0 745 3630
Size: 745 2885
EaeI y'GGCC_r
Cuts at: 0 17 1839 2297 2573 3630
Size: 17 1822 458 276 1057
Fragments arranged by size:
1822 1057 458 276 17
EagI C'GGCC_G
Cuts at: 0 17 3630
Size: 17 3613
EarI CTCTTCn'nnn_
Cuts at: 0 2002 2588 3630
Size: 2002 586 1042
Fragments arranged by size:
2002 1042 586
EcoICRI GAG'CTC
Cuts at: 0 3107 3630
Size: 3107 523
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 136 415 1645 2712 2800 3054 3630
Size: 94 42 279 1230 1067 88 254 576
Fragments arranged by size:
1230 1067 576 279 254 94 88 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1624 1690 3630
Size: 1624 66 1940
Fragments arranged by size:
1940 1624 66
EcoO109I rG'GnC_Cy
Cuts at: 0 724 991 1445 1548 1955 1956 3378 3630
Size: 724 267 454 103 407 1 1422 252
Fragments arranged by size:
1422 724 454 407 267 252 103 1
EcoRI G'AATT_C
Cuts at: 0 293 2131 2899 3630
Size: 293 1838 768 731
Fragments arranged by size:
1838 768 731 293
HaeII r_GCGC'y
Cuts at: 0 31 40 440 666 2013 2931 3227 3630
Size: 31 9 400 226 1347 918 296 403
Fragments arranged by size:
1347 918 403 400 296 226 31 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1030 1062 1437 1469 1555 1587
Size: 898 32 100 32 375 32 86 32
Cuts at: 1587 2177 2209 3630
Size: 590 32 1421
Fragments arranged by size:
1421 898 590 375 100 86 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 3441 3630
Size: 3441 189
KasI G'GCGC_C
Cuts at: 0 436 2009 3223 3630
Size: 436 1573 1214 407
Fragments arranged by size:
1573 1214 436 407
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1933 3008 3630
Size: 1933 1075 622
Fragments arranged by size:
1933 1075 622
MscI TGG'CCA
Cuts at: 0 2299 2575 3630
Size: 2299 276 1055
Fragments arranged by size:
2299 1055 276
MslI CAynn'nnrTG
Cuts at: 0 529 871 1945 2681 2811 2836 3394 3630
Size: 529 342 1074 736 130 25 558 236
Fragments arranged by size:
1074 736 558 529 342 236 130 25
MspA1I CmG'CkG
Cuts at: 0 557 1493 3304 3574 3630
Size: 557 936 1811 270 56
Fragments arranged by size:
1811 936 557 270 56
NaeI GCC'GGC
Cuts at: 0 1839 3630
Size: 1839 1791
NarI GG'CG_CC
Cuts at: 0 437 2010 3224 3630
Size: 437 1573 1214 406
Fragments arranged by size:
1573 1214 437 406
NdeI CA'TA_TG
Cuts at: 0 2742 2812 3630
Size: 2742 70 818
Fragments arranged by size:
2742 818 70
NgoMIV G'CCGG_C
Cuts at: 0 1837 3630
Size: 1837 1793
NsiI A_TGCA'T
Cuts at: 0 2963 3630
Size: 2963 667
NspI r_CATG'y
Cuts at: 0 528 2551 3630
Size: 528 2023 1079
Fragments arranged by size:
2023 1079 528
PasI CC'CwG_GG
Cuts at: 0 1983 3630
Size: 1983 1647
PciI A'CATG_T
Cuts at: 0 524 3630
Size: 524 3106
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 1915 3630
Size: 252 1663 1715
Fragments arranged by size:
1715 1663 252
PfoI T'CCnGG_A
Cuts at: 0 10 245 2393 2405 2777 3630
Size: 10 235 2148 12 372 853
Fragments arranged by size:
2148 853 372 235 12 10
PmlI CAC'GTG
Cuts at: 0 776 2319 3630
Size: 776 1543 1311
Fragments arranged by size:
1543 1311 776
PpuMI rG'GwC_Cy
Cuts at: 0 1445 3378 3630
Size: 1445 1933 252
Fragments arranged by size:
1933 1445 252
PsiI TTA'TAA
Cuts at: 0 1471 3630
Size: 1471 2159
PspOMI G'GGCC_C
Cuts at: 0 1548 1955 3470 3630
Size: 1548 407 1515 160
Fragments arranged by size:
1548 1515 407 160
PstI C_TGCA'G
Cuts at: 0 314 714 2051 3396 3630
Size: 314 400 1337 1345 234
Fragments arranged by size:
1345 1337 400 314 234
PvuII CAG'CTG
Cuts at: 0 557 1493 3574 3630
Size: 557 936 2081 56
Fragments arranged by size:
2081 936 557 56
SacI G_AGCT'C
Cuts at: 0 3109 3630
Size: 3109 521
SfcI C'TryA_G
Cuts at: 0 310 710 2047 2975 3392 3630
Size: 310 400 1337 928 417 238
Fragments arranged by size:
1337 928 417 400 310 238
SfoI GGC'GCC
Cuts at: 0 438 2011 3225 3630
Size: 438 1573 1214 405
Fragments arranged by size:
1573 1214 438 405
SmaI CCC'GGG
Cuts at: 0 730 3630
Size: 730 2900
SmlI C'TyrA_G
Cuts at: 0 1357 2109 3630
Size: 1357 752 1521
Fragments arranged by size:
1521 1357 752
SspI AAT'ATT
Cuts at: 0 1065 2424 3630
Size: 1065 1359 1206
Fragments arranged by size:
1359 1206 1065
StyI C'CwwG_G
Cuts at: 0 93 183 1340 1559 1636 3533 3630
Size: 93 90 1157 219 77 1897 97
Fragments arranged by size:
1897 1157 219 97 93 90 77
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1691 2094 2575 2823 3630
Size: 1691 403 481 248 807
Fragments arranged by size:
1691 807 481 403 248
TatI w'GTAC_w
Cuts at: 0 332 3630
Size: 332 3298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1134 1166 2609 2641 3630
Size: 1134 32 1443 32 989
Fragments arranged by size:
1443 1134 989 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 499 964 1567 2401 3630
Size: 499 465 603 834 1229
Fragments arranged by size:
1229 834 603 499 465
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 249 253 825 1947 2526 3630
Size: 249 4 572 1122 579 1104
Fragments arranged by size:
1122 1104 579 572 249 4
XmaI C'CCGG_G
Cuts at: 0 728 3630
Size: 728 2902
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI
PmlI PpuMI PsiI PspOMI PstI PvuII SacI SfcI
SfoI SmaI SmlI SspI StyI TaqII TatI TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AlfI AscI AseI
AsiSI AvrII BlpI BmtI BsaBI BsiWI BspEI BspHI
BsrBI BsrGI BssHII BstBI BstZ17I CspCI EciI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NcoI NheI NotI NruI PacI PmeI PpiI
PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI XbaI XhoI XmnI
ZraI