Restriction map for
pF1KB4461
(Linear) (Six-base) MAPSORT of: KIBB4461.seq Check: 3053 from: 1 to: 1722
KIBB4461.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1722
Size: 27 1695
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1722
Size: 1421 301
AfeI AGC'GCT
Cuts at: 0 627 1722
Size: 627 1095
AflIII A'CryG_T
Cuts at: 0 371 1722
Size: 371 1351
BanI G'GyrC_C
Cuts at: 0 16 92 663 1078 1722
Size: 16 76 571 415 644
Fragments arranged by size:
644 571 415 76 16
BciVI GTATCCnnnnn_n'
Cuts at: 0 1333 1497 1722
Size: 1333 164 225
Fragments arranged by size:
1333 225 164
BclI T'GATC_A
Cuts at: 0 842 848 959 1722
Size: 842 6 111 763
Fragments arranged by size:
842 763 111 6
BglI GCCn_nnn'nGGC
Cuts at: 0 347 1300 1722
Size: 347 953 422
Fragments arranged by size:
953 422 347
Bme1580I G_kGCm'C
Cuts at: 0 352 1081 1722
Size: 352 729 641
Fragments arranged by size:
729 641 352
BmrI ACTGGGnnnn_n'
Cuts at: 0 409 946 1322 1722
Size: 409 537 376 400
Fragments arranged by size:
537 409 400 376
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 721 1722
Size: 279 442 1001
Fragments arranged by size:
1001 442 279
Bpu10I CC'TnA_GC
Cuts at: 0 329 1722
Size: 329 1393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1722
Size: 430 1292
BsaBI GATnn'nnATC
Cuts at: 0 685 847 1722
Size: 685 162 875
Fragments arranged by size:
875 685 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1018 1048 1722
Size: 1018 30 674
Fragments arranged by size:
1018 674 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 685 1342 1722
Size: 622 63 657 380
Fragments arranged by size:
657 622 380 63
BseYI C'CCAG_C
Cuts at: 0 120 456 1468 1517 1722
Size: 120 336 1012 49 205
Fragments arranged by size:
1012 336 205 120 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1604 1722
Size: 1604 118
BsmI GAATG_Cn'
Cuts at: 0 56 1651 1722
Size: 56 1595 71
Fragments arranged by size:
1595 71 56
Bsp1286I G_dGCh'C
Cuts at: 0 352 1081 1722
Size: 352 729 641
Fragments arranged by size:
729 641 352
BspHI T'CATG_A
Cuts at: 0 254 845 1722
Size: 254 591 877
Fragments arranged by size:
877 591 254
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 1722
Size: 27 1695
BsrDI GCAATG_nn'
Cuts at: 0 386 746 899 1722
Size: 386 360 153 823
Fragments arranged by size:
823 386 360 153
BsrFI r'CCGG_y
Cuts at: 0 89 1722
Size: 89 1633
BsrGI T'GTAC_A
Cuts at: 0 944 1722
Size: 944 778
BssSI C'ACGA_G
Cuts at: 0 351 1722
Size: 351 1371
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1527 1722
Size: 1527 195
BstBI TT'CG_AA
Cuts at: 0 516 1722
Size: 516 1206
BstEII G'GTnAC_C
Cuts at: 0 1341 1722
Size: 1341 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 795 1191 1246 1722
Size: 39 756 396 55 476
Fragments arranged by size:
756 476 396 55 39
BtgI C'CryG_G
Cuts at: 0 359 978 1367 1722
Size: 359 619 389 355
Fragments arranged by size:
619 389 359 355
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 744 1722
Size: 744 978
BtsI GCAGTG_nn'
Cuts at: 0 179 1067 1695 1722
Size: 179 888 628 27
Fragments arranged by size:
888 628 179 27
EaeI y'GGCC_r
Cuts at: 0 339 1316 1722
Size: 339 977 406
Fragments arranged by size:
977 406 339
EarI CTCTTCn'nnn_
Cuts at: 0 529 1722
Size: 529 1193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 721 1421 1722
Size: 279 442 700 301
Fragments arranged by size:
700 442 301 279
EcoO109I rG'GnC_Cy
Cuts at: 0 460 696 1722
Size: 460 236 1026
Fragments arranged by size:
1026 460 236
HaeII r_GCGC'y
Cuts at: 0 629 1722
Size: 629 1093
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 765 797 1464 1496 1539 1571
Size: 469 32 264 32 667 32 43 32
Cuts at: 1571 1722
Size: 151
Fragments arranged by size:
667 469 264 151 43 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 1058 1722
Size: 1058 664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1155 1722
Size: 1155 567
MslI CAynn'nnrTG
Cuts at: 0 215 259 763 850 1189 1722
Size: 215 44 504 87 339 533
Fragments arranged by size:
533 504 339 215 87 44
MspA1I CmG'CkG
Cuts at: 0 456 1722
Size: 456 1266
NcoI C'CATG_G
Cuts at: 0 359 1722
Size: 359 1363
NspI r_CATG'y
Cuts at: 0 375 1722
Size: 375 1347
PasI CC'CwG_GG
Cuts at: 0 241 867 1074 1722
Size: 241 626 207 648
Fragments arranged by size:
648 626 241 207
PciI A'CATG_T
Cuts at: 0 371 1722
Size: 371 1351
PfoI T'CCnGG_A
Cuts at: 0 503 1256 1722
Size: 503 753 466
Fragments arranged by size:
753 503 466
PstI C_TGCA'G
Cuts at: 0 396 1690 1722
Size: 396 1294 32
Fragments arranged by size:
1294 396 32
PvuII CAG'CTG
Cuts at: 0 456 1722
Size: 456 1266
ScaI AGT'ACT
Cuts at: 0 398 1722
Size: 398 1324
SfcI C'TryA_G
Cuts at: 0 392 1686 1722
Size: 392 1294 36
Fragments arranged by size:
1294 392 36
SmlI C'TyrA_G
Cuts at: 0 445 1722
Size: 445 1277
StuI AGG'CCT
Cuts at: 0 320 968 1722
Size: 320 648 754
Fragments arranged by size:
754 648 320
StyI C'CwwG_G
Cuts at: 0 359 1705 1722
Size: 359 1346 17
Fragments arranged by size:
1346 359 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1441 1722
Size: 1441 281
TatI w'GTAC_w
Cuts at: 0 396 944 1722
Size: 396 548 778
Fragments arranged by size:
778 548 396
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1259 1291 1722
Size: 1259 32 431
Fragments arranged by size:
1259 431 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 795 1335 1497 1722
Size: 795 540 162 225
Fragments arranged by size:
795 540 225 162
Enzymes that do cut:
AarI AcuI AfeI AflIII BanI BciVI BclI BglI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoO109I HaeII Hin4I HindIII
MmeI MslI MspA1I NcoI NspI PasI PciI PfoI
PstI PvuII ScaI SfcI SmlI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BfrBI BglII BlpI BmgBI BmtI BplI
BsaI BsaAI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI
BspEI BsrBI BssHII BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI