Restriction map for
pF1KB4465
(Linear) (Six-base) MAPSORT of: KIBB4465.seq Check: 756 from: 1 to: 6963
KIBB4465.orf
With 182 enzymes: SgfI *
May 1, 2012 15:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 526 640 991 1208 1489 2284 2515 2734
Size: 526 114 351 217 281 795 231 219
Cuts at: 2734 2740 2968 3550 6540 6963
Size: 6 228 582 2990 423
Fragments arranged by size:
2990 795 582 526 423 351 281 231
228 219 217 114 6
AatII G_ACGT'C
Cuts at: 0 2301 6963
Size: 2301 4662
Acc65I G'GTAC_C
Cuts at: 0 865 6963
Size: 865 6098
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1455 1479 5444 6963
Size: 1455 24 3965 1519
Fragments arranged by size:
3965 1519 1455 24
AfeI AGC'GCT
Cuts at: 0 4198 6963
Size: 4198 2765
AflIII A'CryG_T
Cuts at: 0 1507 3317 6963
Size: 1507 1810 3646
Fragments arranged by size:
3646 1810 1507
AleI CACnn'nnGTG
Cuts at: 0 961 1840 3544 4379 6400 6963
Size: 961 879 1704 835 2021 563
Fragments arranged by size:
2021 1704 961 879 835 563
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 187 2910 4642 5552 5641 6963
Size: 187 2723 1732 910 89 1322
Fragments arranged by size:
2723 1732 1322 910 187 89
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4904 4936 6963
Size: 4904 32 2027
Fragments arranged by size:
4904 2027 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2196 2772 2795 2997 4322 5329 6823 6963
Size: 2196 576 23 202 1325 1007 1494 140
Fragments arranged by size:
2196 1494 1325 1007 576 202 140 23
ApaI G_GGCC'C
Cuts at: 0 15 413 1316 1955 2983 3521 4707 6089
Size: 15 398 903 639 1028 538 1186 1382
Cuts at: 6089 6230 6271 6341 6377 6519 6926 6963
Size: 141 41 70 36 142 407 37
Fragments arranged by size:
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142 141 70 41 37 36 15
AseI AT'TA_AT
Cuts at: 0 3476 6963
Size: 3476 3487
AvaI C'yCGr_G
Cuts at: 0 179 1488 2082 2201 2691 2965 3656 3792
Size: 179 1309 594 119 490 274 691 136
Cuts at: 3792 3981 4135 4700 4723 6199 6230 6264 6963
Size: 189 154 565 23 1476 31 34 699
Fragments arranged by size:
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189 179 154 136 119 34 31 23
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3579 3612 6963
Size: 3579 33 3351
Fragments arranged by size:
3579 3351 33
BanI G'GyrC_C
Cuts at: 0 298 320 391 514 594 628 865 1192
Size: 298 22 71 123 80 34 237 327
Cuts at: 1192 1555 2695 3524 3538 3634 4119 4131 4157
Size: 363 1140 829 14 96 485 12 26
Cuts at: 4157 4301 4509 5286 6446 6567 6963
Size: 144 208 777 1160 121 396
Fragments arranged by size:
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298 237 208 144 123 121 96 80
71 34 26 22 14 12
BanII G_rGCy'C
Cuts at: 0 15 413 500 1240 1316 1955 2108 2608
Size: 15 398 87 740 76 639 153 500
Cuts at: 2608 2871 2983 3521 4045 4707 5406 5487 5774
Size: 263 112 538 524 662 699 81 287
Cuts at: 5774 6089 6230 6271 6341 6377 6519 6926 6963
Size: 315 141 41 70 36 142 407 37
Fragments arranged by size:
740 699 662 639 538 524 500 407
398 315 287 263 153 142 141 112
87 81 76 70 41 37 36 15
BbeI G_GCGC'C
Cuts at: 0 1196 1559 4123 4305 6450 6571 6963
Size: 1196 363 2564 182 2145 121 392
Fragments arranged by size:
2564 2145 1196 392 363 182 121
BbsI GAAGACnn'nnnn_
Cuts at: 0 664 3197 3322 4656 5652 6963
Size: 664 2533 125 1334 996 1311
Fragments arranged by size:
2533 1334 1311 996 664 125
BbvCI CC'TCA_GC
Cuts at: 0 370 5222 6963
Size: 370 4852 1741
Fragments arranged by size:
4852 1741 370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 330 934 968 2946 2980 3399 3433
Size: 296 34 604 34 1978 34 419 34
Cuts at: 3433 4417 4451 5308 5342 6963
Size: 984 34 857 34 1621
Fragments arranged by size:
1978 1621 984 857 604 419 296 34
34 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3580 6963
Size: 3580 3383
BclI T'GATC_A
Cuts at: 0 4791 6963
Size: 4791 2172
BfrBI ATG'CAT
Cuts at: 0 3410 6963
Size: 3410 3553
BglI GCCn_nnn'nGGC
Cuts at: 0 206 329 443 512 1025 1490 1778 1781
Size: 206 123 114 69 513 465 288 3
Cuts at: 1781 1907 1940 1997 2054 2399 2597 2741 2855
Size: 126 33 57 57 345 198 144 114
Cuts at: 2855 3221 3899 3927 4177 4522 5363 5707 5746
Size: 366 678 28 250 345 841 344 39
Cuts at: 5746 5866 6225 6234 6832 6963
Size: 120 359 9 598 131
Fragments arranged by size:
841 678 598 513 465 366 359 345
345 344 288 250 206 198 144 131
126 123 120 114 114 69 57 57
39 33 28 9 3
BlpI GC'TnA_GC
Cuts at: 0 4255 4603 5486 6963
Size: 4255 348 883 1477
Fragments arranged by size:
4255 1477 883 348
Bme1580I G_kGCm'C
Cuts at: 0 15 202 325 387 396 413 599 631
Size: 15 187 123 62 9 17 186 32
Cuts at: 631 787 1316 1618 1729 1955 2100 2698 2983
Size: 156 529 302 111 226 145 598 285
Cuts at: 2983 3217 3521 3834 3895 4162 4707 5179 5499
Size: 234 304 313 61 267 545 472 320
Cuts at: 5499 6089 6230 6271 6341 6377 6506 6519 6678
Size: 590 141 41 70 36 129 13 159
Cuts at: 6678 6858 6926 6963
Size: 180 68 37
Fragments arranged by size:
598 590 545 529 472 320 313 304
302 285 267 234 226 187 186 180
159 156 145 141 129 123 111 70
68 62 61 41 37 36 32 17
15 13 9
BmgBI CAC'GTC
Cuts at: 0 474 822 1521 3366 4884 5055 6963
Size: 474 348 699 1845 1518 171 1908
Fragments arranged by size:
1908 1845 1518 699 474 348 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 3062 5841 6074 6710 6771 6839 6860 6963
Size: 3062 2779 233 636 61 68 21 103
Fragments arranged by size:
3062 2779 636 233 103 68 61 21
BmtI G_CTAG'C
Cuts at: 0 100 5456 6963
Size: 100 5356 1507
Fragments arranged by size:
5356 1507 100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3396 3645 5055 6963
Size: 3396 249 1410 1908
Fragments arranged by size:
3396 1908 1410 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 2190 3172 4771 4892 4921 5428 6119
Size: 253 1937 982 1599 121 29 507 691
Cuts at: 6119 6479 6524 6963
Size: 360 45 439
Fragments arranged by size:
1937 1599 982 691 507 439 360 253
121 45 29
Bpu10I CC'TnA_GC
Cuts at: 0 370 2674 3630 5222 6755 6902 6963
Size: 370 2304 956 1592 1533 147 61
Fragments arranged by size:
2304 1592 1533 956 370 147 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6388 6963
Size: 6388 575
BsaI GGTCTCn'nnnn_
Cuts at: 0 1279 4146 5911 6963
Size: 1279 2867 1765 1052
Fragments arranged by size:
2867 1765 1279 1052
BsaAI yAC'GTr
Cuts at: 0 1334 1508 2712 6963
Size: 1334 174 1204 4251
Fragments arranged by size:
4251 1334 1204 174
BsaBI GATnn'nnATC
Cuts at: 0 5671 6963
Size: 5671 1292
BsaHI Gr'CG_yC
Cuts at: 0 761 1058 1193 1556 2207 2298 2924 3090
Size: 761 297 135 363 651 91 626 166
Cuts at: 3090 3902 3919 4120 4302 6447 6568 6963
Size: 812 17 201 182 2145 121 395
Fragments arranged by size:
2145 812 761 651 626 395 363 297
201 182 166 135 121 91 17
BsaWI w'CCGG_w
Cuts at: 0 1107 6963
Size: 1107 5856
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1122 1152 1630 1660 2060 2090 2701 2731
Size: 1122 30 478 30 400 30 611 30
Cuts at: 2731 3145 3175 3874 3904 4027 4057 4141 4171
Size: 414 30 699 30 123 30 84 30
Cuts at: 4171 6452 6482 6963
Size: 2281 30 481
Fragments arranged by size:
2281 1122 699 611 481 478 414 400
123 84 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2302 4162 4486 4579 5218 5239 5929 6670
Size: 2302 1860 324 93 639 21 690 741
Cuts at: 6670 6963
Size: 293
Fragments arranged by size:
2302 1860 741 690 639 324 293 93
21
BseYI C'CCAG_C
Cuts at: 0 167 209 882 2219 2788 3269 3383 3456
Size: 167 42 673 1337 569 481 114 73
Cuts at: 3456 3755 3833 4232 4481 4915 5103 5581 6213
Size: 299 78 399 249 434 188 478 632
Cuts at: 6213 6578 6670 6816 6866 6889 6914 6918 6926
Size: 365 92 146 50 23 25 4 8
Cuts at: 6926 6963
Size: 37
Fragments arranged by size:
1337 673 632 569 481 478 434 399
365 299 249 188 167 146 114 92
78 73 50 42 37 25 23 8
4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 1461 1869 2247 2256 2307 2460 2487
Size: 795 666 408 378 9 51 153 27
Cuts at: 2487 2712 2940 3030 3555 5029 5611 6031 6963
Size: 225 228 90 525 1474 582 420 932
Fragments arranged by size:
1474 932 795 666 582 525 420 408
378 228 225 153 90 51 27 9
BsiEI CG_ry'CG
Cuts at: 0 1119 1260 1344 4022 4206 4446 6963
Size: 1119 141 84 2678 184 240 2517
Fragments arranged by size:
2678 2517 1119 240 184 141 84
BsiHKAI G_wGCw'C
Cuts at: 0 1186 1211 1713 3467 4544 5012 5774 6725
Size: 1186 25 502 1754 1077 468 762 951
Cuts at: 6725 6761 6963
Size: 36 202
Fragments arranged by size:
1754 1186 1077 951 762 502 468 202
36 25
BsmI GAATG_Cn'
Cuts at: 0 1534 1759 6963
Size: 1534 225 5204
Fragments arranged by size:
5204 1534 225
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2198 6609 6963
Size: 2198 4411 354
Fragments arranged by size:
4411 2198 354
Bsp1286I G_dGCh'C
Cuts at: 0 15 202 325 387 396 413 500 599
Size: 15 187 123 62 9 17 87 99
Cuts at: 599 631 787 1186 1211 1240 1316 1618 1713
Size: 32 156 399 25 29 76 302 95
Cuts at: 1713 1729 1955 2100 2108 2608 2698 2871 2983
Size: 16 226 145 8 500 90 173 112
Cuts at: 2983 3217 3467 3521 3834 3895 4045 4162 4544
Size: 234 250 54 313 61 150 117 382
Cuts at: 4544 4707 5012 5179 5406 5487 5499 5774 6089
Size: 163 305 167 227 81 12 275 315
Cuts at: 6089 6230 6271 6341 6377 6506 6519 6678 6725
Size: 141 41 70 36 129 13 159 47
Cuts at: 6725 6761 6858 6926 6963
Size: 36 97 68 37
Fragments arranged by size:
500 399 382 315 313 305 302 275
250 234 227 226 187 173 167 163
159 156 150 145 141 129 123 117
112 99 97 95 90 87 81 76
70 68 62 61 54 47 41 37
36 36 32 29 25 17 16 15
13 12 9 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 526 640 877 991 1208 1489 2284 2515
Size: 526 114 237 114 217 281 795 231
Cuts at: 2515 2734 2740 2780 2968 3301 3550 6540 6963
Size: 219 6 40 188 333 249 2990 423
Fragments arranged by size:
2990 795 526 423 333 281 249 237
231 219 217 188 114 114 40 6
BsrBI CCG'CTC
Cuts at: 0 222 3086 3861 4062 5684 6618 6963
Size: 222 2864 775 201 1622 934 345
Fragments arranged by size:
2864 1622 934 775 345 222 201
BsrDI GCAATG_nn'
Cuts at: 0 4285 6963
Size: 4285 2678
BsrFI r'CCGG_y
Cuts at: 0 317 2233 2638 4378 4512 4676 4763 5075
Size: 317 1916 405 1740 134 164 87 312
Cuts at: 5075 6963
Size: 1888
Fragments arranged by size:
1916 1888 1740 405 317 312 164 134
87
BssHII G'CGCG_C
Cuts at: 0 3868 4088 4164 6963
Size: 3868 220 76 2799
Fragments arranged by size:
3868 2799 220 76
BssSI C'ACGA_G
Cuts at: 0 1081 2586 3418 6963
Size: 1081 1505 832 3545
Fragments arranged by size:
3545 1505 1081 832
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1076 1544 1733 1883 2684 6862 6963
Size: 1076 468 189 150 801 4178 101
Fragments arranged by size:
4178 1076 801 468 189 150 101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1566 1579 4381 6457 6963
Size: 1566 13 2802 2076 506
Fragments arranged by size:
2802 2076 1566 506 13
BstYI r'GATC_y
Cuts at: 0 360 6963
Size: 360 6603
Bsu36I CC'TnA_GG
Cuts at: 0 5030 6963
Size: 5030 1933
BtgI C'CryG_G
Cuts at: 0 15 267 327 387 507 590 972 1022
Size: 15 252 60 60 120 83 382 50
Cuts at: 1022 1665 1776 2511 3297 3924 4035 4127 4153
Size: 643 111 735 786 627 111 92 26
Cuts at: 4153 4527 4658 5087 5294 6022 6108 6302 6322
Size: 374 131 429 207 728 86 194 20
Cuts at: 6322 6836 6963
Size: 514 127
Fragments arranged by size:
786 735 728 643 627 514 429 382
374 252 207 194 131 127 120 111
111 92 86 83 60 60 50 26
20 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1671 2267 2672 5760 6963
Size: 1671 596 405 3088 1203
Fragments arranged by size:
3088 1671 1203 596 405
BtsI GCAGTG_nn'
Cuts at: 0 2268 2757 2871 2992 3184 3895 4300 4794
Size: 2268 489 114 121 192 711 405 494
Cuts at: 4794 4873 4933 5041 6389 6395 6407 6821 6856
Size: 79 60 108 1348 6 12 414 35
Cuts at: 6856 6963
Size: 107
Fragments arranged by size:
2268 1348 711 494 489 414 405 192
121 114 108 107 79 60 35 12
6
ClaI AT'CG_AT
Cuts at: 0 944 6963
Size: 944 6019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5334 5369 6860 6895 6963
Size: 5334 35 1491 35 68
Fragments arranged by size:
5334 1491 68 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 580 1622 2458 5174 6963
Size: 580 1042 836 2716 1789
Fragments arranged by size:
2716 1789 1042 836 580
DrdI GACnn_nn'nnGTC
Cuts at: 0 731 1063 3738 6286 6963
Size: 731 332 2675 2548 677
Fragments arranged by size:
2675 2548 731 677 332
EaeI y'GGCC_r
Cuts at: 0 18 264 888 1116 2370 4019 4383 4443
Size: 18 246 624 228 1254 1649 364 60
Cuts at: 4443 4650 5120 5150 5933 6839 6963
Size: 207 470 30 783 906 124
Fragments arranged by size:
1649 1254 906 783 624 470 364 246
228 207 124 60 30 18
EagI C'GGCC_G
Cuts at: 0 1116 4019 4443 6963
Size: 1116 2903 424 2520
Fragments arranged by size:
2903 2520 1116 424
EarI CTCTTCn'nnn_
Cuts at: 0 4312 5420 5762 6178 6963
Size: 4312 1108 342 416 785
Fragments arranged by size:
4312 1108 785 416 342
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3695 4624 6963
Size: 3695 929 2339
Fragments arranged by size:
3695 2339 929
EcoICRI GAG'CTC
Cuts at: 0 5772 6963
Size: 5772 1191
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1455 1479 2190 3172 4771 4892 4921
Size: 253 1202 24 711 982 1599 121 29
Cuts at: 4921 5428 5444 6119 6479 6524 6963
Size: 507 16 675 360 45 439
Fragments arranged by size:
1599 1202 982 711 675 507 439 360
253 121 45 29 24 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 6901 6963
Size: 6901 62
EcoO109I rG'GnC_Cy
Cuts at: 0 11 237 253 340 409 1312 1313 1951
Size: 11 226 16 87 69 903 1 638
Cuts at: 1951 1952 2769 2810 3056 3290 3517 3518 3672
Size: 1 817 41 246 234 227 1 154
Cuts at: 3672 3718 3796 3961 6086 6118 6140 6194 6226
Size: 46 78 165 2125 32 22 54 32
Cuts at: 6226 6227 6268 6338 6373 6374 6544 6574 6596
Size: 1 41 70 35 1 170 30 22
Cuts at: 6596 6922 6923 6963
Size: 326 1 40
Fragments arranged by size:
2125 903 817 638 326 246 234 227
226 170 165 154 87 78 70 69
54 46 41 41 40 35 32 32
30 22 22 16 11 1 1 1
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 6717 6963
Size: 6717 246
FspI TGC'GCA
Cuts at: 0 4618 6963
Size: 4618 2345
HaeII r_GCGC'y
Cuts at: 0 79 1196 1559 2936 4123 4200 4291 4305
Size: 79 1117 363 1377 1187 77 91 14
Cuts at: 4305 4586 4610 4820 4857 4952 6450 6571 6963
Size: 281 24 210 37 95 1498 121 392
Fragments arranged by size:
1498 1377 1187 1117 392 363 281 210
121 95 91 79 77 37 24 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 410 442 918 950 1168 1200 1291 1323
Size: 410 32 476 32 218 32 91 32
Cuts at: 1323 1630 1662 5044 5076 5431 5463 6455 6487
Size: 307 32 3382 32 355 32 992 32
Cuts at: 6487 6963
Size: 476
Fragments arranged by size:
3382 992 476 476 410 355 307 218
91 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 765 1296 1437 6963
Size: 765 531 141 5526
Fragments arranged by size:
5526 765 531 141
HindIII A'AGCT_T
Cuts at: 0 3108 6963
Size: 3108 3855
KasI G'GCGC_C
Cuts at: 0 1192 1555 4119 4301 6446 6567 6963
Size: 1192 363 2564 182 2145 121 396
Fragments arranged by size:
2564 2145 1192 396 363 182 121
KpnI G_GTAC'C
Cuts at: 0 869 6963
Size: 869 6094
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 5489 5954 6963
Size: 5489 465 1009
Fragments arranged by size:
5489 1009 465
MscI TGG'CCA
Cuts at: 0 890 2372 5122 5152 6841 6963
Size: 890 1482 2750 30 1689 122
Fragments arranged by size:
2750 1689 1482 890 122 30
MslI CAynn'nnrTG
Cuts at: 0 318 961 1052 1085 1840 2093 3544 4379
Size: 318 643 91 33 755 253 1451 835
Cuts at: 4379 5281 5789 6400 6963
Size: 902 508 611 563
Fragments arranged by size:
1451 902 835 755 643 611 563 508
318 253 91 33
MspA1I CmG'CkG
Cuts at: 0 171 231 398 461 545 559 592 837
Size: 171 60 167 63 84 14 33 245
Cuts at: 837 896 1475 1499 1515 1595 1820 2039 2150
Size: 59 579 24 16 80 225 219 111
Cuts at: 2150 2183 2354 2376 2501 2840 2954 3159 3507
Size: 33 171 22 125 339 114 205 348
Cuts at: 3507 3569 3926 4013 4079 4129 4155 4322 4448
Size: 62 357 87 66 50 26 167 126
Cuts at: 4448 4529 4599 4822 4846 4899 4926 5635 6024
Size: 81 70 223 24 53 27 709 389
Cuts at: 6024 6324 6385 6582 6918 6963
Size: 300 61 197 336 45
Fragments arranged by size:
709 579 389 357 348 339 336 300
245 225 223 219 205 197 171 171
167 167 126 125 114 111 87 84
81 80 70 66 63 62 61 60
59 53 50 45 33 33 27 26
24 24 22 16 14
NaeI GCC'GGC
Cuts at: 0 2640 4514 5077 6963
Size: 2640 1874 563 1886
Fragments arranged by size:
2640 1886 1874 563
NarI GG'CG_CC
Cuts at: 0 1193 1556 4120 4302 6447 6568 6963
Size: 1193 363 2564 182 2145 121 395
Fragments arranged by size:
2564 2145 1193 395 363 182 121
NcoI C'CATG_G
Cuts at: 0 507 972 1022 1665 1776 2511 4658 5294
Size: 507 465 50 643 111 735 2147 636
Cuts at: 5294 6836 6963
Size: 1542 127
Fragments arranged by size:
2147 1542 735 643 636 507 465 127
111 50
NgoMIV G'CCGG_C
Cuts at: 0 2638 4512 5075 6963
Size: 2638 1874 563 1888
Fragments arranged by size:
2638 1888 1874 563
NheI G'CTAG_C
Cuts at: 0 96 5452 6963
Size: 96 5356 1511
Fragments arranged by size:
5356 1511 96
NotI GC'GGCC_GC
Cuts at: 0 4019 6963
Size: 4019 2944
NsiI A_TGCA'T
Cuts at: 0 3412 6963
Size: 3412 3551
NspI r_CATG'y
Cuts at: 0 752 1163 2246 3321 5901 6963
Size: 752 411 1083 1075 2580 1062
Fragments arranged by size:
2580 1083 1075 1062 752 411
PasI CC'CwG_GG
Cuts at: 0 2397 3949 5652 6592 6963
Size: 2397 1552 1703 940 371
Fragments arranged by size:
2397 1703 1552 940 371
PciI A'CATG_T
Cuts at: 0 3317 6963
Size: 3317 3646
PflMI CCAn_nnn'nTGG
Cuts at: 0 1173 2196 2997 4659 5159 6114 6649 6963
Size: 1173 1023 801 1662 500 955 535 314
Fragments arranged by size:
1662 1173 1023 955 801 535 500 314
PfoI T'CCnGG_A
Cuts at: 0 720 6963
Size: 720 6243
PmlI CAC'GTG
Cuts at: 0 1334 1508 2712 6963
Size: 1334 174 1204 4251
Fragments arranged by size:
4251 1334 1204 174
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2569 2601 6963
Size: 2569 32 4362
Fragments arranged by size:
4362 2569 32
PpuMI rG'GwC_Cy
Cuts at: 0 237 2769 2810 3290 3672 3718 3796 3961
Size: 237 2532 41 480 382 46 78 165
Cuts at: 3961 6574 6596 6963
Size: 2613 22 367
Fragments arranged by size:
2613 2532 480 382 367 237 165 78
46 41 22
PshAI GACnn'nnGTC
Cuts at: 0 242 716 1291 1415 6601 6963
Size: 242 474 575 124 5186 362
Fragments arranged by size:
5186 575 474 362 242 124
PspOMI G'GGCC_C
Cuts at: 0 11 409 1312 1951 2979 3517 4703 6085
Size: 11 398 903 639 1028 538 1186 1382
Cuts at: 6085 6226 6267 6337 6373 6515 6922 6963
Size: 141 41 70 36 142 407 41
Fragments arranged by size:
1382 1186 1028 903 639 538 407 398
142 141 70 41 41 36 11
PstI C_TGCA'G
Cuts at: 0 117 637 842 934 1549 2113 2188 2785
Size: 117 520 205 92 615 564 75 597
Cuts at: 2785 2932 3121 3671 3890 4295 4339 4598 4778
Size: 147 189 550 219 405 44 259 180
Cuts at: 4778 6402 6963
Size: 1624 561
Fragments arranged by size:
1624 615 597 564 561 550 520 405
259 219 205 189 180 147 117 92
75 44
PvuII CAG'CTG
Cuts at: 0 171 231 461 559 837 896 1475 1515
Size: 171 60 230 98 278 59 579 40
Cuts at: 1515 2376 2501 2840 3159 4013 5635 6385 6582
Size: 861 125 339 319 854 1622 750 197
Cuts at: 6582 6918 6963
Size: 336 45
Fragments arranged by size:
1622 861 854 750 579 339 336 319
278 230 197 171 125 98 60 59
45 40
SacI G_AGCT'C
Cuts at: 0 5774 6963
Size: 5774 1189
SacII CC_GC'GG
Cuts at: 0 593 3927 4130 4156 4530 6025 6325 6963
Size: 593 3334 203 26 374 1495 300 638
Fragments arranged by size:
3334 1495 638 593 374 300 203 26
SanDI GG'GwC_CC
Cuts at: 0 3961 6574 6596 6963
Size: 3961 2613 22 367
Fragments arranged by size:
3961 2613 367 22
SapI GCTCTTCn'nnn_
Cuts at: 0 5762 6178 6963
Size: 5762 416 785
Fragments arranged by size:
5762 785 416
SbfI CC_TGCA'GG
Cuts at: 0 637 1549 3121 3671 6963
Size: 637 912 1572 550 3292
Fragments arranged by size:
3292 1572 912 637 550
ScaI AGT'ACT
Cuts at: 0 4420 6963
Size: 4420 2543
SexAI A'CCwGG_T
Cuts at: 0 1796 3551 6963
Size: 1796 1755 3412
Fragments arranged by size:
3412 1796 1755
SfcI C'TryA_G
Cuts at: 0 113 633 838 930 1545 2109 2184 2781
Size: 113 520 205 92 615 564 75 597
Cuts at: 2781 2928 3117 3667 3886 4291 4335 4594 4774
Size: 147 189 550 219 405 44 259 180
Cuts at: 4774 5687 6398 6963
Size: 913 711 565
Fragments arranged by size:
913 711 615 597 565 564 550 520
405 259 219 205 189 180 147 113
92 75 44
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 4522 6225 6963
Size: 4522 1703 738
Fragments arranged by size:
4522 1703 738
SfoI GGC'GCC
Cuts at: 0 1194 1557 4121 4303 6448 6569 6963
Size: 1194 363 2564 182 2145 121 394
Fragments arranged by size:
2564 2145 1194 394 363 182 121
SgrAI Cr'CCGG_yG
Cuts at: 0 4378 6963
Size: 4378 2585
SmaI CCC'GGG
Cuts at: 0 181 2693 3658 3794 3983 4702 6201 6232
Size: 181 2512 965 136 189 719 1499 31
Cuts at: 6232 6266 6963
Size: 34 697
Fragments arranged by size:
2512 1499 965 719 697 189 181 136
34 31
SmlI C'TyrA_G
Cuts at: 0 2965 6367 6963
Size: 2965 3402 596
Fragments arranged by size:
3402 2965 596
SrfI GCCC'GGGC
Cuts at: 0 2693 6266 6963
Size: 2693 3573 697
Fragments arranged by size:
3573 2693 697
StyI C'CwwG_G
Cuts at: 0 507 972 1022 1665 1776 2511 4658 5123
Size: 507 465 50 643 111 735 2147 465
Cuts at: 5123 5294 6836 6963
Size: 171 1542 127
Fragments arranged by size:
2147 1542 735 643 507 465 465 171
127 111 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 181 201 205 481 874 1303 1472 3547
Size: 181 20 4 276 393 429 169 2075
Cuts at: 3547 4969 6276 6963
Size: 1422 1307 687
Fragments arranged by size:
2075 1422 1307 687 429 393 276 181
169 20 4
TatI w'GTAC_w
Cuts at: 0 4418 6468 6963
Size: 4418 2050 495
Fragments arranged by size:
4418 2050 495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1122 1154 1824 1856 2060 2092 2701 2733
Size: 1122 32 670 32 204 32 609 32
Cuts at: 2733 3145 3177 3282 3314 3874 3906 4027 4059
Size: 412 32 105 32 560 32 121 32
Cuts at: 4059 4141 4173 5158 5190 6963
Size: 82 32 985 32 1773
Fragments arranged by size:
1773 1122 985 670 609 560 412 204
121 105 82 32 32 32 32 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1459 1927 6963
Size: 1459 468 5036
Fragments arranged by size:
5036 1459 468
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 556 1258 1512 4229 5160 5571 5970 6834
Size: 556 702 254 2717 931 411 399 864
Cuts at: 6834 6963
Size: 129
Fragments arranged by size:
2717 931 864 702 556 411 399 254
129
XhoI C'TCGA_G
Cuts at: 0 2965 6963
Size: 2965 3998
XmaI C'CCGG_G
Cuts at: 0 179 2691 3656 3792 3981 4700 6199 6230
Size: 179 2512 965 136 189 719 1499 31
Cuts at: 6230 6264 6963
Size: 34 699
Fragments arranged by size:
2512 1499 965 719 699 189 179 136
34 31
XmnI GAAnn'nnTTC
Cuts at: 0 2833 6963
Size: 2833 4130
ZraI GAC'GTC
Cuts at: 0 2299 6963
Size: 2299 4664
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AleI AlfI
AloI AlwNI ApaI AseI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgrAI SmaI SmlI SrfI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AgeI AhdI ApaLI ApoI AscI
AsiSI AvrII BamHI BglII BsiWI BspEI BspHI BsrGI
BstBI BstEII BstZ17I DraI EcoRI EcoRV FseI FspAI
HpaI MfeI MluI NdeI NruI PacI PmeI PsiI
PspXI PsrI PvuI RsrII SalI SgfI SnaBI SpeI
SphI SspI StuI SwaI XbaI