Restriction map for
pF1KB4477
(Linear) (Six-base) MAPSORT of: KIBB4477.seq Check: 9982 from: 1 to: 3447
KIBB4477.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AatII G_ACGT'C
Cuts at: 0 1322 3447
Size: 1322 2125
Acc65I G'GTAC_C
Cuts at: 0 437 3447
Size: 437 3010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 3331 3381 3447
Size: 127 3204 50 66
Fragments arranged by size:
3204 127 66 50
AfeI AGC'GCT
Cuts at: 0 2191 3447
Size: 2191 1256
AflIII A'CryG_T
Cuts at: 0 3248 3447
Size: 3248 199
AhdI GACnn_n'nnGTC
Cuts at: 0 2277 3045 3447
Size: 2277 768 402
Fragments arranged by size:
2277 768 402
AleI CACnn'nnGTG
Cuts at: 0 1693 2324 3447
Size: 1693 631 1123
Fragments arranged by size:
1693 1123 631
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 970 1002 3447
Size: 970 32 2445
Fragments arranged by size:
2445 970 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 2773 3447
Size: 180 2593 674
Fragments arranged by size:
2593 674 180
ApaI G_GGCC'C
Cuts at: 0 2662 3447
Size: 2662 785
ApaLI G'TGCA_C
Cuts at: 0 2636 3447
Size: 2636 811
AvaI C'yCGr_G
Cuts at: 0 974 1279 1377 2267 2384 2472 2538 2808
Size: 974 305 98 890 117 88 66 270
Cuts at: 2808 3080 3447
Size: 272 367
Fragments arranged by size:
974 890 367 305 272 270 117 98
88 66
BanI G'GyrC_C
Cuts at: 0 437 2647 3130 3447
Size: 437 2210 483 317
Fragments arranged by size:
2210 483 437 317
BanII G_rGCy'C
Cuts at: 0 683 983 2073 2092 2479 2662 2809 3447
Size: 683 300 1090 19 387 183 147 638
Fragments arranged by size:
1090 683 638 387 300 183 147 19
BbeI G_GCGC'C
Cuts at: 0 3134 3447
Size: 3134 313
BbsI GAAGACnn'nnnn_
Cuts at: 0 874 1888 2295 2439 3041 3447
Size: 874 1014 407 144 602 406
Fragments arranged by size:
1014 874 602 407 406 144
BclI T'GATC_A
Cuts at: 0 1865 3447
Size: 1865 1582
BglI GCCn_nnn'nGGC
Cuts at: 0 1442 2830 3447
Size: 1442 1388 617
Fragments arranged by size:
1442 1388 617
BglII A'GATC_T
Cuts at: 0 1094 1143 3350 3447
Size: 1094 49 2207 97
Fragments arranged by size:
2207 1094 97 49
BlpI GC'TnA_GC
Cuts at: 0 478 2600 3447
Size: 478 2122 847
Fragments arranged by size:
2122 847 478
Bme1580I G_kGCm'C
Cuts at: 0 1992 2321 2640 2650 2662 3447
Size: 1992 329 319 10 12 785
Fragments arranged by size:
1992 785 329 319 12 10
BmgBI CAC'GTC
Cuts at: 0 1695 3447
Size: 1695 1752
BmrI ACTGGGnnnn_n'
Cuts at: 0 224 3447
Size: 224 3223
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 447 1754 2935 3447
Size: 447 1307 1181 512
Fragments arranged by size:
1307 1181 512 447
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1138 1268 1644 2166 2463 2807 3095
Size: 442 696 130 376 522 297 344 288
Cuts at: 3095 3247 3346 3447
Size: 152 99 101
Fragments arranged by size:
696 522 442 376 344 297 288 152
130 101 99
Bpu10I CC'TnA_GC
Cuts at: 0 1207 2091 2631 3447
Size: 1207 884 540 816
Fragments arranged by size:
1207 884 816 540
BsaI GGTCTCn'nnnn_
Cuts at: 0 2108 3447
Size: 2108 1339
BsaHI Gr'CG_yC
Cuts at: 0 1319 1598 3131 3447
Size: 1319 279 1533 316
Fragments arranged by size:
1533 1319 316 279
BsaWI w'CCGG_w
Cuts at: 0 2099 3447
Size: 2099 1348
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 601 631 970 1000 1288 1318 1492 1522
Size: 601 30 339 30 288 30 174 30
Cuts at: 1522 2236 2266 2353 2383 2680 2710 3447
Size: 714 30 87 30 297 30 737
Fragments arranged by size:
737 714 601 339 297 288 174 87
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 606 2176 2179 2683 2688 2697 3397 3447
Size: 606 1570 3 504 5 9 700 50
Fragments arranged by size:
1570 700 606 504 50 9 5 3
BseYI C'CCAG_C
Cuts at: 0 383 798 1584 2022 2041 2053 2899 2988
Size: 383 415 786 438 19 12 846 89
Cuts at: 2988 3447
Size: 459
Fragments arranged by size:
846 786 459 438 415 383 89 19
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 973 1259 3447
Size: 954 19 286 2188
Fragments arranged by size:
2188 954 286 19
BsiEI CG_ry'CG
Cuts at: 0 2825 3447
Size: 2825 622
BsiHKAI G_wGCw'C
Cuts at: 0 1679 2640 2936 3447
Size: 1679 961 296 511
Fragments arranged by size:
1679 961 511 296
BsmBI CGTCTCn'nnnn_
Cuts at: 0 618 3447
Size: 618 2829
Bsp1286I G_dGCh'C
Cuts at: 0 683 983 1679 1992 2073 2092 2321 2479
Size: 683 300 696 313 81 19 229 158
Cuts at: 2479 2640 2650 2662 2809 2936 3447
Size: 161 10 12 147 127 511
Fragments arranged by size:
696 683 511 313 300 229 161 158
147 127 81 19 12 10
BspHI T'CATG_A
Cuts at: 0 923 1007 3447
Size: 923 84 2440
Fragments arranged by size:
2440 923 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1202 2508 2754 3447
Size: 1202 1306 246 693
Fragments arranged by size:
1306 1202 693 246
BsrBI CCG'CTC
Cuts at: 0 193 3117 3447
Size: 193 2924 330
Fragments arranged by size:
2924 330 193
BsrDI GCAATG_nn'
Cuts at: 0 123 465 1765 2052 3447
Size: 123 342 1300 287 1395
Fragments arranged by size:
1395 1300 342 287 123
BsrFI r'CCGG_y
Cuts at: 0 1049 1502 1954 2155 2334 3232 3447
Size: 1049 453 452 201 179 898 215
Fragments arranged by size:
1049 898 453 452 215 201 179
BsrGI T'GTAC_A
Cuts at: 0 1079 3447
Size: 1079 2368
BssSI C'ACGA_G
Cuts at: 0 2859 3447
Size: 2859 588
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2773 3447
Size: 2773 674
BstEII G'GTnAC_C
Cuts at: 0 2379 2520 3447
Size: 2379 141 927
Fragments arranged by size:
2379 927 141
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 2865 3147 3447
Size: 894 1971 282 300
Fragments arranged by size:
1971 894 300 282
BstYI r'GATC_y
Cuts at: 0 1094 1143 3350 3447
Size: 1094 49 2207 97
Fragments arranged by size:
2207 1094 97 49
Bsu36I CC'TnA_GG
Cuts at: 0 173 1036 2580 3447
Size: 173 863 1544 867
Fragments arranged by size:
1544 867 863 173
BtgI C'CryG_G
Cuts at: 0 441 887 1067 1106 1602 2226 2282 2525
Size: 441 446 180 39 496 624 56 243
Cuts at: 2525 3447
Size: 922
Fragments arranged by size:
922 624 496 446 441 243 180 56
39
BtsI GCAGTG_nn'
Cuts at: 0 111 587 1492 2580 3227 3378 3447
Size: 111 476 905 1088 647 151 69
Fragments arranged by size:
1088 905 647 476 151 111 69
DraIII CAC_nnn'GTG
Cuts at: 0 1185 2246 3447
Size: 1185 1061 1201
Fragments arranged by size:
1201 1185 1061
EaeI y'GGCC_r
Cuts at: 0 148 275 375 531 641 671 860 1085
Size: 148 127 100 156 110 30 189 225
Cuts at: 1085 1932 2822 2939 3206 3383 3447
Size: 847 890 117 267 177 64
Fragments arranged by size:
890 847 267 225 189 177 156 148
127 117 110 100 64 30
EagI C'GGCC_G
Cuts at: 0 2822 3447
Size: 2822 625
EarI CTCTTCn'nnn_
Cuts at: 0 893 1958 2344 3095 3447
Size: 893 1065 386 751 352
Fragments arranged by size:
1065 893 751 386 352
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2199 2949 3447
Size: 2199 750 498
Fragments arranged by size:
2199 750 498
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 442 1138 1268 1644 2166 2463 2807
Size: 127 315 696 130 376 522 297 344
Cuts at: 2807 3095 3247 3331 3346 3381 3447
Size: 288 152 84 15 35 66
Fragments arranged by size:
696 522 376 344 315 297 288 152
130 127 84 66 35 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1393 3447
Size: 1393 2054
EcoO109I rG'GnC_Cy
Cuts at: 0 387 1629 1655 1789 2058 2585 2658 2659
Size: 387 1242 26 134 269 527 73 1
Cuts at: 2659 2916 2975 3447
Size: 257 59 472
Fragments arranged by size:
1242 527 472 387 269 257 134 73
59 26 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1146 1866 3318 3447
Size: 1146 720 1452 129
Fragments arranged by size:
1452 1146 720 129
HaeII r_GCGC'y
Cuts at: 0 1437 1824 1862 2193 3134 3172 3447
Size: 1437 387 38 331 941 38 275
Fragments arranged by size:
1437 941 387 331 275 38 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1492 1524 2236 2263 2268 2295 2353 2385
Size: 1492 32 712 27 5 27 58 32
Cuts at: 2385 3031 3063 3299 3331 3447
Size: 646 32 236 32 116
Fragments arranged by size:
1492 712 646 236 116 58 32 32
32 32 27 27 5
HincII GTy'rAC
Cuts at: 0 414 3447
Size: 414 3033
HindIII A'AGCT_T
Cuts at: 0 45 3447
Size: 45 3402
KasI G'GCGC_C
Cuts at: 0 3130 3447
Size: 3130 317
KpnI G_GTAC'C
Cuts at: 0 441 3447
Size: 441 3006
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1340 3447
Size: 178 1162 2107
Fragments arranged by size:
2107 1162 178
MscI TGG'CCA
Cuts at: 0 277 377 533 1087 3208 3447
Size: 277 100 156 554 2121 239
Fragments arranged by size:
2121 554 277 239 156 100
MslI CAynn'nnrTG
Cuts at: 0 116 784 892 1693 2324 3447
Size: 116 668 108 801 631 1123
Fragments arranged by size:
1123 801 668 631 116 108
MspA1I CmG'CkG
Cuts at: 0 147 466 485 2017 2599 3128 3382 3447
Size: 147 319 19 1532 582 529 254 65
Fragments arranged by size:
1532 582 529 319 254 147 65 19
NaeI GCC'GGC
Cuts at: 0 1956 2336 3234 3447
Size: 1956 380 898 213
Fragments arranged by size:
1956 898 380 213
NarI GG'CG_CC
Cuts at: 0 3131 3447
Size: 3131 316
NcoI C'CATG_G
Cuts at: 0 441 887 1106 2226 3447
Size: 441 446 219 1120 1221
Fragments arranged by size:
1221 1120 446 441 219
NgoMIV G'CCGG_C
Cuts at: 0 1954 2334 3232 3447
Size: 1954 380 898 215
Fragments arranged by size:
1954 898 380 215
NspI r_CATG'y
Cuts at: 0 1368 1481 3447
Size: 1368 113 1966
Fragments arranged by size:
1966 1368 113
PflMI CCAn_nnn'nTGG
Cuts at: 0 1268 3447
Size: 1268 2179
PfoI T'CCnGG_A
Cuts at: 0 1025 1547 1660 1919 2075 3152 3338 3447
Size: 1025 522 113 259 156 1077 186 109
Fragments arranged by size:
1077 1025 522 259 186 156 113 109
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 970 1002 3447
Size: 970 32 2445
Fragments arranged by size:
2445 970 32
PpuMI rG'GwC_Cy
Cuts at: 0 387 1655 1789 2058 2585 2975 3447
Size: 387 1268 134 269 527 390 472
Fragments arranged by size:
1268 527 472 390 387 269 134
PspOMI G'GGCC_C
Cuts at: 0 2658 3447
Size: 2658 789
PstI C_TGCA'G
Cuts at: 0 921 1628 1674 2598 2778 3447
Size: 921 707 46 924 180 669
Fragments arranged by size:
924 921 707 669 180 46
PvuII CAG'CTG
Cuts at: 0 2017 2599 3447
Size: 2017 582 848
Fragments arranged by size:
2017 848 582
SanDI GG'GwC_CC
Cuts at: 0 387 2058 2585 2975 3447
Size: 387 1671 527 390 472
Fragments arranged by size:
1671 527 472 390 387
ScaI AGT'ACT
Cuts at: 0 23 3447
Size: 23 3424
SexAI A'CCwGG_T
Cuts at: 0 947 2375 3447
Size: 947 1428 1072
Fragments arranged by size:
1428 1072 947
SfcI C'TryA_G
Cuts at: 0 917 1624 1670 2594 2774 3447
Size: 917 707 46 924 180 673
Fragments arranged by size:
924 917 707 673 180 46
SfoI GGC'GCC
Cuts at: 0 3132 3447
Size: 3132 315
SgrAI Cr'CCGG_yG
Cuts at: 0 2155 3447
Size: 2155 1292
SmaI CCC'GGG
Cuts at: 0 976 1379 2474 3447
Size: 976 403 1095 973
Fragments arranged by size:
1095 976 973 403
SrfI GCCC'GGGC
Cuts at: 0 2474 3447
Size: 2474 973
StyI C'CwwG_G
Cuts at: 0 206 378 441 887 960 1106 1715 2006
Size: 206 172 63 446 73 146 609 291
Cuts at: 2006 2226 2234 3140 3447
Size: 220 8 906 307
Fragments arranged by size:
906 609 446 307 291 220 206 172
146 73 63 8
TatI w'GTAC_w
Cuts at: 0 21 1079 3447
Size: 21 1058 2368
Fragments arranged by size:
2368 1058 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2230 2262 2680 2712 3447
Size: 2230 32 418 32 735
Fragments arranged by size:
2230 735 418 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 370 1113 3321 3447
Size: 370 743 2208 126
Fragments arranged by size:
2208 743 370 126
XmaI C'CCGG_G
Cuts at: 0 974 1377 2472 3447
Size: 974 403 1095 975
Fragments arranged by size:
1095 975 974 403
XmnI GAAnn'nnTTC
Cuts at: 0 140 215 1129 3447
Size: 140 75 914 2318
Fragments arranged by size:
2318 914 140 75
ZraI GAC'GTC
Cuts at: 0 1320 3447
Size: 1320 2127
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PflMI PfoI PpiI PpuMI PspOMI
PstI PvuII SanDI ScaI SexAI SfcI SfoI SgrAI
SmaI SrfI StyI TatI TstI XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AccI AclI AflII AgeI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI
BfrBI BmtI BpuEI BsaAI BsaBI BsiWI BsmI BspEI
BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI
EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI MfeI
MluI NdeI NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI SfiI SgfI
SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII
Tth111I XbaI XhoI