Restriction map for
pF1KB4506
(Linear) (Six-base) MAPSORT of: KIBB4506.seq Check: 9762 from: 1 to: 1965
KIBB4506.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 522 1965
Size: 522 1443
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 1965
Size: 1661 304
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1408 1965
Size: 1408 557
AlwNI CAG_nnn'CTG
Cuts at: 0 400 553 1965
Size: 400 153 1412
Fragments arranged by size:
1412 400 153
ApaI G_GGCC'C
Cuts at: 0 1859 1965
Size: 1859 106
AvaI C'yCGr_G
Cuts at: 0 1502 1511 1671 1965
Size: 1502 9 160 294
Fragments arranged by size:
1502 294 160 9
AvrII C'CTAG_G
Cuts at: 0 486 1965
Size: 486 1479
BamHI G'GATC_C
Cuts at: 0 733 1965
Size: 733 1232
BanI G'GyrC_C
Cuts at: 0 522 1965
Size: 522 1443
BanII G_rGCy'C
Cuts at: 0 1859 1965
Size: 1859 106
BbsI GAAGACnn'nnnn_
Cuts at: 0 1070 1256 1842 1965
Size: 1070 186 586 123
Fragments arranged by size:
1070 586 186 123
BclI T'GATC_A
Cuts at: 0 609 1965
Size: 609 1356
BglI GCCn_nnn'nGGC
Cuts at: 0 250 1703 1965
Size: 250 1453 262
Fragments arranged by size:
1453 262 250
BglII A'GATC_T
Cuts at: 0 1029 1965
Size: 1029 936
Bme1580I G_kGCm'C
Cuts at: 0 1859 1965
Size: 1859 106
BmgBI CAC'GTC
Cuts at: 0 1903 1965
Size: 1903 62
BmrI ACTGGGnnnn_n'
Cuts at: 0 809 1184 1965
Size: 809 375 781
Fragments arranged by size:
809 781 375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1744 1965
Size: 1744 221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 998 1547 1574 1623 1965
Size: 866 132 549 27 49 342
Fragments arranged by size:
866 549 342 132 49 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 1965
Size: 62 1903
BsaBI GATnn'nnATC
Cuts at: 0 1034 1192 1965
Size: 1034 158 773
Fragments arranged by size:
1034 773 158
BsaHI Gr'CG_yC
Cuts at: 0 4 1965
Size: 4 1961
BsaWI w'CCGG_w
Cuts at: 0 856 872 1965
Size: 856 16 1093
Fragments arranged by size:
1093 856 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 543 573 867 897 1388 1418 1523 1553
Size: 543 30 294 30 491 30 105 30
Cuts at: 1553 1965
Size: 412
Fragments arranged by size:
543 491 412 294 105 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 968 1484 1493 1544 1910 1965
Size: 968 516 9 51 366 55
Fragments arranged by size:
968 516 366 55 51 9
BseYI C'CCAG_C
Cuts at: 0 68 481 1965
Size: 68 413 1484
Fragments arranged by size:
1484 413 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 539 1965
Size: 386 153 1426
Fragments arranged by size:
1426 386 153
BsiHKAI G_wGCw'C
Cuts at: 0 1937 1965
Size: 1937 28
Bsp1286I G_dGCh'C
Cuts at: 0 1859 1937 1965
Size: 1859 78 28
Fragments arranged by size:
1859 78 28
BspEI T'CCGG_A
Cuts at: 0 856 1965
Size: 856 1109
BsrGI T'GTAC_A
Cuts at: 0 1089 1965
Size: 1089 876
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 1965
Size: 400 1565
BstXI CCAn_nnnn'nTGG
Cuts at: 0 106 277 526 822 1965
Size: 106 171 249 296 1143
Fragments arranged by size:
1143 296 249 171 106
BstYI r'GATC_y
Cuts at: 0 733 1029 1053 1965
Size: 733 296 24 912
Fragments arranged by size:
912 733 296 24
BtgI C'CryG_G
Cuts at: 0 8 1013 1274 1407 1445 1464 1770 1794
Size: 8 1005 261 133 38 19 306 24
Cuts at: 1794 1965
Size: 171
Fragments arranged by size:
1005 306 261 171 133 38 24 19
8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1244 1965
Size: 1244 721
BtsI GCAGTG_nn'
Cuts at: 0 170 302 398 551 1150 1259 1570 1965
Size: 170 132 96 153 599 109 311 395
Fragments arranged by size:
599 395 311 170 153 132 109 96
DraIII CAC_nnn'GTG
Cuts at: 0 1378 1408 1965
Size: 1378 30 557
Fragments arranged by size:
1378 557 30
DrdI GACnn_nn'nnGTC
Cuts at: 0 1448 1965
Size: 1448 517
EaeI y'GGCC_r
Cuts at: 0 1767 1800 1965
Size: 1767 33 165
Fragments arranged by size:
1767 165 33
EarI CTCTTCn'nnn_
Cuts at: 0 903 1713 1887 1965
Size: 903 810 174 78
Fragments arranged by size:
903 810 174 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1655 1965
Size: 1655 310
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 998 1547 1574 1623 1661 1965
Size: 866 132 549 27 49 38 304
Fragments arranged by size:
866 549 304 132 49 38 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1204 1493 1965
Size: 1204 289 472
Fragments arranged by size:
1204 472 289
EcoO109I rG'GnC_Cy
Cuts at: 0 1432 1441 1498 1629 1855 1856 1909 1965
Size: 1432 9 57 131 226 1 53 56
Fragments arranged by size:
1432 226 131 57 56 53 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1342 1965
Size: 1342 623
HaeII r_GCGC'y
Cuts at: 0 977 1965
Size: 977 988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1523 1555 1965
Size: 1051 32 440 32 410
Fragments arranged by size:
1051 440 410 32 32
HincII GTy'rAC
Cuts at: 0 448 682 1965
Size: 448 234 1283
Fragments arranged by size:
1283 448 234
KpnI G_GTAC'C
Cuts at: 0 526 1965
Size: 526 1439
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 825 1965
Size: 262 563 1140
Fragments arranged by size:
1140 563 262
NcoI C'CATG_G
Cuts at: 0 1013 1445 1965
Size: 1013 432 520
Fragments arranged by size:
1013 520 432
NdeI CA'TA_TG
Cuts at: 0 105 351 1965
Size: 105 246 1614
Fragments arranged by size:
1614 246 105
NspI r_CATG'y
Cuts at: 0 1395 1710 1965
Size: 1395 315 255
Fragments arranged by size:
1395 315 255
PasI CC'CwG_GG
Cuts at: 0 1849 1965
Size: 1849 116
PflMI CCAn_nnn'nTGG
Cuts at: 0 379 1408 1965
Size: 379 1029 557
Fragments arranged by size:
1029 557 379
PfoI T'CCnGG_A
Cuts at: 0 1416 1965
Size: 1416 549
PpuMI rG'GwC_Cy
Cuts at: 0 1432 1441 1498 1909 1965
Size: 1432 9 57 411 56
Fragments arranged by size:
1432 411 57 56 9
PspOMI G'GGCC_C
Cuts at: 0 1855 1965
Size: 1855 110
PstI C_TGCA'G
Cuts at: 0 39 1965
Size: 39 1926
SanDI GG'GwC_CC
Cuts at: 0 1441 1965
Size: 1441 524
SfcI C'TryA_G
Cuts at: 0 24 35 51 308 507 738 1049 1965
Size: 24 11 16 257 199 231 311 916
Fragments arranged by size:
916 311 257 231 199 24 16 11
SmaI CCC'GGG
Cuts at: 0 1504 1673 1965
Size: 1504 169 292
Fragments arranged by size:
1504 292 169
SmlI C'TyrA_G
Cuts at: 0 77 1965
Size: 77 1888
SpeI A'CTAG_T
Cuts at: 0 442 601 712 1965
Size: 442 159 111 1253
Fragments arranged by size:
1253 442 159 111
SphI G_CATG'C
Cuts at: 0 1395 1710 1965
Size: 1395 315 255
Fragments arranged by size:
1395 315 255
StuI AGG'CCT
Cuts at: 0 328 1965
Size: 328 1637
StyI C'CwwG_G
Cuts at: 0 486 1013 1265 1445 1965
Size: 486 527 252 180 520
Fragments arranged by size:
527 520 486 252 180
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 112 337 814 1272 1829 1965
Size: 112 225 477 458 557 136
Fragments arranged by size:
557 477 458 225 136 112
TatI w'GTAC_w
Cuts at: 0 1089 1965
Size: 1089 876
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 543 575 867 899 1388 1420 1965
Size: 543 32 292 32 489 32 545
Fragments arranged by size:
545 543 489 292 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1116 1965
Size: 1116 849
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1272 1452 1549 1965
Size: 1272 180 97 416
Fragments arranged by size:
1272 416 180 97
XmaI C'CCGG_G
Cuts at: 0 1502 1671 1965
Size: 1502 169 294
Fragments arranged by size:
1502 294 169
Enzymes that do cut:
Acc65I AcuI AlfI AlwNI ApaI AvaI AvrII BamHI
BanI BanII BbsI BclI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BsrGI BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII KpnI MmeI NcoI NdeI NspI PasI PflMI
PfoI PpuMI PspOMI PstI SanDI SfcI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BbeI BbvCI BcgI BciVI BfrBI BlpI BmtI
Bpu10I BsaI BsaAI BsiEI BsiWI BsmI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI EagI EcoICRI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI
MluI MscI MslI MspA1I NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SrfI SspI SwaI Tth111I XhoI
XmnI ZraI