Restriction map for
pF1KB4514
(Linear) (Six-base) MAPSORT of: KIBB4514.seq Check: 2377 from: 1 to: 4860
KIBB4514.orf
With 182 enzymes: SgfI *
October 24, 2006 15:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1070 2620 3098 4860
Size: 1070 1550 478 1762
Fragments arranged by size:
1762 1550 1070 478
AccI GT'mk_AC
Cuts at: 0 197 2975 4073 4860
Size: 197 2778 1098 787
Fragments arranged by size:
2778 1098 787 197
Acc65I G'GTAC_C
Cuts at: 0 4704 4860
Size: 4704 156
AclI AA'CG_TT
Cuts at: 0 4631 4860
Size: 4631 229
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 400 1285 3343 3479 4130 4860
Size: 259 141 885 2058 136 651 730
Fragments arranged by size:
2058 885 730 651 259 141 136
AflIII A'CryG_T
Cuts at: 0 1688 2059 2561 4422 4860
Size: 1688 371 502 1861 438
Fragments arranged by size:
1861 1688 502 438 371
AhdI GACnn_n'nnGTC
Cuts at: 0 3612 3936 4860
Size: 3612 324 924
Fragments arranged by size:
3612 924 324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2711 4860
Size: 2711 2149
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 321 353 1513 1545 3473 3505 4860
Size: 321 32 1160 32 1928 32 1355
Fragments arranged by size:
1928 1355 1160 321 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1095 1820 2966 3195 3761 3784 4860
Size: 1095 725 1146 229 566 23 1076
Fragments arranged by size:
1146 1095 1076 725 566 229 23
ApaI G_GGCC'C
Cuts at: 0 470 2078 2155 4859 4860
Size: 470 1608 77 2704 1
Fragments arranged by size:
2704 1608 470 77 1
ApaLI G'TGCA_C
Cuts at: 0 1051 4860
Size: 1051 3809
ApoI r'AATT_y
Cuts at: 0 2004 3517 4860
Size: 2004 1513 1343
Fragments arranged by size:
2004 1513 1343
AvaI C'yCGr_G
Cuts at: 0 119 203 253 472 571 1996 2208 3642
Size: 119 84 50 219 99 1425 212 1434
Cuts at: 3642 3689 3821 4860
Size: 47 132 1039
Fragments arranged by size:
1434 1425 1039 219 212 132 119 99
84 50 47
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 4696 4729 4860
Size: 4696 33 131
Fragments arranged by size:
4696 131 33
BamHI G'GATC_C
Cuts at: 0 557 1852 4480 4860
Size: 557 1295 2628 380
Fragments arranged by size:
2628 1295 557 380
BanI G'GyrC_C
Cuts at: 0 177 312 869 1058 2181 2505 2695 3330
Size: 177 135 557 189 1123 324 190 635
Cuts at: 3330 4704 4860
Size: 1374 156
Fragments arranged by size:
1374 1123 635 557 324 190 189 177
156 135
BanII G_rGCy'C
Cuts at: 0 17 61 91 470 1318 1636 1649 2078
Size: 17 44 30 379 848 318 13 429
Cuts at: 2078 2155 3226 3593 4676 4859 4860
Size: 77 1071 367 1083 183 1
Fragments arranged by size:
1083 1071 848 429 379 367 318 183
77 44 30 17 13 1
BbeI G_GCGC'C
Cuts at: 0 316 873 4860
Size: 316 557 3987
Fragments arranged by size:
3987 557 316
BbsI GAAGACnn'nnnn_
Cuts at: 0 3454 4118 4860
Size: 3454 664 742
Fragments arranged by size:
3454 742 664
BbvCI CC'TCA_GC
Cuts at: 0 975 3044 4860
Size: 975 2069 1816
Fragments arranged by size:
2069 1816 975
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3670 3704 4860
Size: 3670 34 1156
Fragments arranged by size:
3670 1156 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4809 4860
Size: 4809 51
BclI T'GATC_A
Cuts at: 0 2513 3506 4860
Size: 2513 993 1354
Fragments arranged by size:
2513 1354 993
BglI GCCn_nnn'nGGC
Cuts at: 0 1867 2548 3133 4239 4860
Size: 1867 681 585 1106 621
Fragments arranged by size:
1867 1106 681 621 585
BglII A'GATC_T
Cuts at: 0 653 2171 4860
Size: 653 1518 2689
Fragments arranged by size:
2689 1518 653
BlpI GC'TnA_GC
Cuts at: 0 3238 4860
Size: 3238 1622
Bme1580I G_kGCm'C
Cuts at: 0 182 470 1055 1386 2078 2155 2510 4859
Size: 182 288 585 331 692 77 355 2349
Cuts at: 4859 4860
Size: 1
Fragments arranged by size:
2349 692 585 355 331 288 182 77
1
BmrI ACTGGGnnnn_n'
Cuts at: 0 2345 2893 4649 4860
Size: 2345 548 1756 211
Fragments arranged by size:
2345 1756 548 211
BmtI G_CTAG'C
Cuts at: 0 274 4860
Size: 274 4586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 909 1141 2607 4860
Size: 909 232 1466 2253
Fragments arranged by size:
2253 1466 909 232
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 481 811 841 1124 1150 1206 1330
Size: 448 33 330 30 283 26 56 124
Cuts at: 1330 2314 2602 3607 4036 4624 4817 4860
Size: 984 288 1005 429 588 193 43
Fragments arranged by size:
1005 984 588 448 429 330 288 283
193 124 56 43 33 30 26
Bpu10I CC'TnA_GC
Cuts at: 0 295 975 2128 3044 4860
Size: 295 680 1153 916 1816
Fragments arranged by size:
1816 1153 916 680 295
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1698 1788 3595 4860
Size: 1698 90 1807 1265
Fragments arranged by size:
1807 1698 1265 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 327 3599 3622 4281 4386 4860
Size: 327 3272 23 659 105 474
Fragments arranged by size:
3272 659 474 327 105 23
BsaAI yAC'GTr
Cuts at: 0 2478 3684 4425 4860
Size: 2478 1206 741 435
Fragments arranged by size:
2478 1206 741 435
BsaHI Gr'CG_yC
Cuts at: 0 313 583 870 1182 2342 3053 4860
Size: 313 270 287 312 1160 711 1807
Fragments arranged by size:
1807 1160 711 313 312 287 270
BsaWI w'CCGG_w
Cuts at: 0 2620 2701 3406 4076 4860
Size: 2620 81 705 670 784
Fragments arranged by size:
2620 784 705 670 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1008 1038 1867 1897 2104 2134 2489 2519
Size: 1008 30 829 30 207 30 355 30
Cuts at: 2519 4860
Size: 2341
Fragments arranged by size:
2341 1008 829 355 207 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 219 454 901 1029 1484 1789 2468 2471
Size: 219 235 447 128 455 305 679 3
Cuts at: 2471 2583 2775 2810 3297 3645 4256 4312 4315
Size: 112 192 35 487 348 611 56 3
Cuts at: 4315 4332 4860
Size: 17 528
Fragments arranged by size:
679 611 528 487 455 447 348 305
235 219 192 128 112 56 35 17
3 3
BseYI C'CCAG_C
Cuts at: 0 93 303 432 632 1088 2277 2901 3991
Size: 93 210 129 200 456 1189 624 1090
Cuts at: 3991 4311 4860
Size: 320 549
Fragments arranged by size:
1189 1090 624 549 456 320 210 200
129 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1017 1604 3664 4140 4860
Size: 816 201 587 2060 476 720
Fragments arranged by size:
2060 816 720 587 476 201
BsiEI CG_ry'CG
Cuts at: 0 4409 4860
Size: 4409 451
BsiHKAI G_wGCw'C
Cuts at: 0 1031 1055 1166 1318 1636 1649 2785 3469
Size: 1031 24 111 152 318 13 1136 684
Cuts at: 3469 3593 4860
Size: 124 1267
Fragments arranged by size:
1267 1136 1031 684 318 152 124 111
24 13
BsiWI C'GTAC_G
Cuts at: 0 4517 4860
Size: 4517 343
BsmI GAATG_Cn'
Cuts at: 0 3416 4435 4860
Size: 3416 1019 425
Fragments arranged by size:
3416 1019 425
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1050 3060 4860
Size: 1050 2010 1800
Fragments arranged by size:
2010 1800 1050
Bsp1286I G_dGCh'C
Cuts at: 0 17 61 91 182 470 1031 1055 1166
Size: 17 44 30 91 288 561 24 111
Cuts at: 1166 1318 1386 1636 1649 2078 2155 2510 2785
Size: 152 68 250 13 429 77 355 275
Cuts at: 2785 3226 3469 3593 4676 4859 4860
Size: 441 243 124 1083 183 1
Fragments arranged by size:
1083 561 441 429 355 288 275 250
243 183 152 124 111 91 77 68
44 30 24 17 13 1
BspEI T'CCGG_A
Cuts at: 0 2620 3406 4860
Size: 2620 786 1454
Fragments arranged by size:
2620 1454 786
BspHI T'CATG_A
Cuts at: 0 3164 3263 4860
Size: 3164 99 1597
Fragments arranged by size:
3164 1597 99
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1070 1494 2620 3098 4860
Size: 1070 424 1126 478 1762
Fragments arranged by size:
1762 1126 1070 478 424
BsrDI GCAATG_nn'
Cuts at: 0 1082 2516 2826 3695 3746 4860
Size: 1082 1434 310 869 51 1114
Fragments arranged by size:
1434 1114 1082 869 310 51
BsrFI r'CCGG_y
Cuts at: 0 73 309 334 2502 4404 4860
Size: 73 236 25 2168 1902 456
Fragments arranged by size:
2168 1902 456 236 73 25
BsrGI T'GTAC_A
Cuts at: 0 2312 2894 4860
Size: 2312 582 1966
Fragments arranged by size:
2312 1966 582
BssHII G'CGCG_C
Cuts at: 0 81 4860
Size: 81 4779
BssSI C'ACGA_G
Cuts at: 0 3130 4582 4860
Size: 3130 1452 278
Fragments arranged by size:
3130 1452 278
BstAPI GCAn_nnn'nTGC
Cuts at: 0 423 3204 3567 4860
Size: 423 2781 363 1293
Fragments arranged by size:
2781 1293 423 363
BstEII G'GTnAC_C
Cuts at: 0 3117 4591 4860
Size: 3117 1474 269
Fragments arranged by size:
3117 1474 269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 1893 3210 3881 4137 4860
Size: 420 1473 1317 671 256 723
Fragments arranged by size:
1473 1317 723 671 420 256
BstYI r'GATC_y
Cuts at: 0 557 653 1121 1852 1928 2171 4480 4860
Size: 557 96 468 731 76 243 2309 380
Fragments arranged by size:
2309 731 557 468 380 243 96 76
BstZ17I GTA'TAC
Cuts at: 0 4074 4860
Size: 4074 786
Bsu36I CC'TnA_GG
Cuts at: 0 847 2787 4253 4860
Size: 847 1940 1466 607
Fragments arranged by size:
1940 1466 847 607
BtgI C'CryG_G
Cuts at: 0 44 261 614 1006 2079 2262 2925 3027
Size: 44 217 353 392 1073 183 663 102
Cuts at: 3027 3334 3369 4409 4476 4860
Size: 307 35 1040 67 384
Fragments arranged by size:
1073 1040 663 392 384 353 307 217
183 102 67 44 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 757 4860
Size: 757 4103
BtsI GCAGTG_nn'
Cuts at: 0 835 1029 1253 1281 1786 4660 4860
Size: 835 194 224 28 505 2874 200
Fragments arranged by size:
2874 835 505 224 200 194 28
DraI TTT'AAA
Cuts at: 0 2909 4860
Size: 2909 1951
DraIII CAC_nnn'GTG
Cuts at: 0 2268 4860
Size: 2268 2592
EaeI y'GGCC_r
Cuts at: 0 75 1040 3134 3662 3794 4406 4860
Size: 75 965 2094 528 132 612 454
Fragments arranged by size:
2094 965 612 528 454 132 75
EagI C'GGCC_G
Cuts at: 0 4406 4860
Size: 4406 454
EarI CTCTTCn'nnn_
Cuts at: 0 148 193 652 3783 4211 4217 4683 4860
Size: 148 45 459 3131 428 6 466 177
Fragments arranged by size:
3131 466 459 428 177 148 45 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 550 2465 2816 4860
Size: 550 1915 351 2044
Fragments arranged by size:
2044 1915 550 351
EcoICRI GAG'CTC
Cuts at: 0 1316 1634 1647 3591 4860
Size: 1316 318 13 1944 1269
Fragments arranged by size:
1944 1316 1269 318 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 400 448 481 811 841 1124 1150
Size: 259 141 48 33 330 30 283 26
Cuts at: 1150 1206 1285 1330 2314 2602 3343 3479 3607
Size: 56 79 45 984 288 741 136 128
Cuts at: 3607 4036 4130 4624 4817 4860
Size: 429 94 494 193 43
Fragments arranged by size:
984 741 494 429 330 288 283 259
193 141 136 128 94 79 56 48
45 43 33 30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1074 2296 3325 4860
Size: 1074 1222 1029 1535
Fragments arranged by size:
1535 1222 1074 1029
EcoO109I rG'GnC_Cy
Cuts at: 0 207 467 1511 2075 2151 2152 2540 3781
Size: 207 260 1044 564 76 1 388 1241
Cuts at: 3781 4164 4248 4655 4860
Size: 383 84 407 205
Fragments arranged by size:
1241 1044 564 407 388 383 260 207
205 84 76 1
FseI GG_CCGG'CC
Cuts at: 0 4408 4860
Size: 4408 452
FspI TGC'GCA
Cuts at: 0 397 4860
Size: 397 4463
HaeII r_GCGC'y
Cuts at: 0 316 873 4860
Size: 316 557 3987
Fragments arranged by size:
3987 557 316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 141 173 542 574 1122 1154 1867 1899
Size: 141 32 369 32 548 32 713 32
Cuts at: 1899 3040 3072 3598 3621 3630 3653 3922 3954
Size: 1141 32 526 23 9 23 269 32
Cuts at: 3954 4071 4103 4860
Size: 117 32 757
Fragments arranged by size:
1141 757 713 548 526 369 269 141
117 32 32 32 32 32 32 32
23 23 9
HincII GTy'rAC
Cuts at: 0 168 4860
Size: 168 4692
KasI G'GCGC_C
Cuts at: 0 312 869 4860
Size: 312 557 3991
Fragments arranged by size:
3991 557 312
KpnI G_GTAC'C
Cuts at: 0 4708 4860
Size: 4708 152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 566 1135 1309 2147 4472 4860
Size: 566 569 174 838 2325 388
Fragments arranged by size:
2325 838 569 566 388 174
MscI TGG'CCA
Cuts at: 0 3664 3796 4860
Size: 3664 132 1064
Fragments arranged by size:
3664 1064 132
MslI CAynn'nnrTG
Cuts at: 0 2251 3169 3208 3550 4860
Size: 2251 918 39 342 1310
Fragments arranged by size:
2251 1310 918 342 39
MspA1I CmG'CkG
Cuts at: 0 33 52 97 111 263 291 616 979
Size: 33 19 45 14 152 28 325 363
Cuts at: 979 1092 2072 3336 4315 4372 4860
Size: 113 980 1264 979 57 488
Fragments arranged by size:
1264 980 979 488 363 325 152 113
57 45 33 28 19 14
NaeI GCC'GGC
Cuts at: 0 311 4406 4860
Size: 311 4095 454
Fragments arranged by size:
4095 454 311
NarI GG'CG_CC
Cuts at: 0 313 870 4860
Size: 313 557 3990
Fragments arranged by size:
3990 557 313
NcoI C'CATG_G
Cuts at: 0 2079 3369 4860
Size: 2079 1290 1491
Fragments arranged by size:
2079 1491 1290
NgoMIV G'CCGG_C
Cuts at: 0 309 4404 4860
Size: 309 4095 456
Fragments arranged by size:
4095 456 309
NheI G'CTAG_C
Cuts at: 0 270 4860
Size: 270 4590
NspI r_CATG'y
Cuts at: 0 699 1750 2063 2310 4860
Size: 699 1051 313 247 2550
Fragments arranged by size:
2550 1051 699 313 247
PasI CC'CwG_GG
Cuts at: 0 1386 4063 4860
Size: 1386 2677 797
Fragments arranged by size:
2677 1386 797
PciI A'CATG_T
Cuts at: 0 2059 4860
Size: 2059 2801
PflMI CCAn_nnn'nTGG
Cuts at: 0 3784 4505 4860
Size: 3784 721 355
Fragments arranged by size:
3784 721 355
PfoI T'CCnGG_A
Cuts at: 0 61 325 1532 4860
Size: 61 264 1207 3328
Fragments arranged by size:
3328 1207 264 61
PmlI CAC'GTG
Cuts at: 0 3684 4425 4860
Size: 3684 741 435
Fragments arranged by size:
3684 741 435
PpuMI rG'GwC_Cy
Cuts at: 0 207 1511 4164 4248 4860
Size: 207 1304 2653 84 612
Fragments arranged by size:
2653 1304 612 207 84
PshAI GACnn'nnGTC
Cuts at: 0 2981 4860
Size: 2981 1879
PspOMI G'GGCC_C
Cuts at: 0 466 2074 2151 4855 4860
Size: 466 1608 77 2704 5
Fragments arranged by size:
2704 1608 466 77 5
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 4501 4533 4860
Size: 4501 32 327
Fragments arranged by size:
4501 327 32
PstI C_TGCA'G
Cuts at: 0 116 149 1067 1248 1276 1313 1829 1937
Size: 116 33 918 181 28 37 516 108
Cuts at: 1937 2962 3221 4377 4860
Size: 1025 259 1156 483
Fragments arranged by size:
1156 1025 918 516 483 259 181 116
108 37 33 28
PvuII CAG'CTG
Cuts at: 0 52 97 979 1092 4315 4372 4860
Size: 52 45 882 113 3223 57 488
Fragments arranged by size:
3223 882 488 113 57 52 45
SacI G_AGCT'C
Cuts at: 0 1318 1636 1649 3593 4860
Size: 1318 318 13 1944 1267
Fragments arranged by size:
1944 1318 1267 318 13
SacII CC_GC'GG
Cuts at: 0 264 617 3337 4860
Size: 264 353 2720 1523
Fragments arranged by size:
2720 1523 353 264
SapI GCTCTTCn'nnn_
Cuts at: 0 193 4860
Size: 193 4667
SfcI C'TryA_G
Cuts at: 0 112 145 1063 1244 1272 1309 1595 1825
Size: 112 33 918 181 28 37 286 230
Cuts at: 1825 1933 2036 2199 2958 3217 3831 3846 4373
Size: 108 103 163 759 259 614 15 527
Cuts at: 4373 4562 4860
Size: 189 298
Fragments arranged by size:
918 759 614 527 298 286 259 230
189 181 163 112 108 103 37 33
28 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2548 4860
Size: 2548 2312
SfoI GGC'GCC
Cuts at: 0 314 871 4860
Size: 314 557 3989
Fragments arranged by size:
3989 557 314
SmaI CCC'GGG
Cuts at: 0 121 205 474 1998 4860
Size: 121 84 269 1524 2862
Fragments arranged by size:
2862 1524 269 121 84
SmlI C'TyrA_G
Cuts at: 0 1677 1767 3610 4860
Size: 1677 90 1843 1250
Fragments arranged by size:
1843 1677 1250 90
SnaBI TAC'GTA
Cuts at: 0 2478 4860
Size: 2478 2382
SspI AAT'ATT
Cuts at: 0 2940 3914 4860
Size: 2940 974 946
Fragments arranged by size:
2940 974 946
StuI AGG'CCT
Cuts at: 0 892 1360 3898 4860
Size: 892 468 2538 962
Fragments arranged by size:
2538 962 892 468
StyI C'CwwG_G
Cuts at: 0 537 677 1470 1694 2079 3369 4244 4860
Size: 537 140 793 224 385 1290 875 616
Fragments arranged by size:
1290 875 793 616 537 385 224 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1962 2104 2585 3087 3283 4065 4174 4860
Size: 1962 142 481 502 196 782 109 686
Fragments arranged by size:
1962 782 686 502 481 196 142 109
TatI w'GTAC_w
Cuts at: 0 2312 2353 2894 3230 4615 4860
Size: 2312 41 541 336 1385 245
Fragments arranged by size:
2312 1385 541 336 245 41
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1008 1040 2489 2521 2873 2905 4860
Size: 1008 32 1449 32 352 32 1955
Fragments arranged by size:
1955 1449 1008 352 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 366 4860
Size: 366 4494
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 1863 2130 3376 4860
Size: 51 1812 267 1246 1484
Fragments arranged by size:
1812 1484 1246 267 51
XmaI C'CCGG_G
Cuts at: 0 119 203 472 1996 4860
Size: 119 84 269 1524 2864
Fragments arranged by size:
2864 1524 269 119 84
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AhdI AlfI
AloI AlwNI ApaI ApaLI ApoI AvaI BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI DraI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI HaeII Hin4I HincII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PasI PciI PflMI PfoI
PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SacI
SacII SapI SfcI SfiI SfoI SmaI SmlI SnaBI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AfeI AflII AgeI AleI AscI AseI AsiSI
AvrII BfrBI BmgBI BsaBI BsrBI BstBI ClaI CspCI
DrdI EcoRI EcoRV FalI FspAI HindIII HpaI MfeI
MluI NdeI NotI NruI NsiI PacI PmeI PpiI
PsiI PspXI PvuI RsrII SalI SanDI SbfI ScaI
SexAI SgfI SgrAI SpeI SphI SrfI SwaI XbaI
XhoI XmnI ZraI