Restriction map for
pF1KB4535
(Linear) (Six-base) MAPSORT of: KIBB4535.seq Check: 279 from: 1 to: 1338
KIBB4535.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 424 858 1338
Size: 424 434 480
Fragments arranged by size:
480 434 424
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1075 1221 1338
Size: 505 570 146 117
Fragments arranged by size:
570 505 146 117
AfeI AGC'GCT
Cuts at: 0 551 940 1338
Size: 551 389 398
Fragments arranged by size:
551 398 389
AflIII A'CryG_T
Cuts at: 0 200 1338
Size: 200 1138
AleI CACnn'nnGTG
Cuts at: 0 919 1280 1338
Size: 919 361 58
Fragments arranged by size:
919 361 58
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 154 1338
Size: 154 1184
AlwNI CAG_nnn'CTG
Cuts at: 0 327 1338
Size: 327 1011
AvaI C'yCGr_G
Cuts at: 0 121 650 1177 1338
Size: 121 529 527 161
Fragments arranged by size:
529 527 161 121
BanI G'GyrC_C
Cuts at: 0 18 74 185 422 1248 1338
Size: 18 56 111 237 826 90
Fragments arranged by size:
826 237 111 90 56 18
BanII G_rGCy'C
Cuts at: 0 1187 1205 1338
Size: 1187 18 133
Fragments arranged by size:
1187 133 18
BbeI G_GCGC'C
Cuts at: 0 78 189 426 1338
Size: 78 111 237 912
Fragments arranged by size:
912 237 111 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 384 418 1338
Size: 384 34 920
Fragments arranged by size:
920 384 34
BglI GCCn_nnn'nGGC
Cuts at: 0 673 1338
Size: 673 665
Bme1580I G_kGCm'C
Cuts at: 0 23 314 1253 1334 1338
Size: 23 291 939 81 4
Fragments arranged by size:
939 291 81 23 4
BmgBI CAC'GTC
Cuts at: 0 201 858 1338
Size: 201 657 480
Fragments arranged by size:
657 480 201
Bpu10I CC'TnA_GC
Cuts at: 0 473 1338
Size: 473 865
BsaI GGTCTCn'nnnn_
Cuts at: 0 379 647 1338
Size: 379 268 691
Fragments arranged by size:
691 379 268
BsaHI Gr'CG_yC
Cuts at: 0 75 186 226 354 423 1338
Size: 75 111 40 128 69 915
Fragments arranged by size:
915 128 111 75 69 40
BsaWI w'CCGG_w
Cuts at: 0 819 1338
Size: 819 519
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 1338
Size: 505 833
BseYI C'CCAG_C
Cuts at: 0 83 126 546 593 636 641 1270 1338
Size: 83 43 420 47 43 5 629 68
Fragments arranged by size:
629 420 83 68 47 43 43 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 847 955 1338
Size: 74 773 108 383
Fragments arranged by size:
773 383 108 74
BsiEI CG_ry'CG
Cuts at: 0 257 300 1338
Size: 257 43 1038
Fragments arranged by size:
1038 257 43
BsiHKAI G_wGCw'C
Cuts at: 0 242 620 1205 1338
Size: 242 378 585 133
Fragments arranged by size:
585 378 242 133
BsmI GAATG_Cn'
Cuts at: 0 525 1338
Size: 525 813
Bsp1286I G_dGCh'C
Cuts at: 0 23 242 314 620 1187 1205 1253 1334
Size: 23 219 72 306 567 18 48 81
Cuts at: 1334 1338
Size: 4
Fragments arranged by size:
567 306 219 81 72 48 23 18
4
BspEI T'CCGG_A
Cuts at: 0 819 1338
Size: 819 519
BspHI T'CATG_A
Cuts at: 0 806 927 1338
Size: 806 121 411
Fragments arranged by size:
806 411 121
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 143 424 858 1338
Size: 143 281 434 480
Fragments arranged by size:
480 434 281 143
BsrFI r'CCGG_y
Cuts at: 0 71 228 1338
Size: 71 157 1110
Fragments arranged by size:
1110 157 71
BstEII G'GTnAC_C
Cuts at: 0 273 768 889 1299 1338
Size: 273 495 121 410 39
Fragments arranged by size:
495 410 273 121 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 36 1338
Size: 36 1302
BstYI r'GATC_y
Cuts at: 0 323 1338
Size: 323 1015
Bsu36I CC'TnA_GG
Cuts at: 0 454 1016 1338
Size: 454 562 322
Fragments arranged by size:
562 454 322
BtgI C'CryG_G
Cuts at: 0 279 1278 1338
Size: 279 999 60
Fragments arranged by size:
999 279 60
BtsI GCAGTG_nn'
Cuts at: 0 115 914 1338
Size: 115 799 424
Fragments arranged by size:
799 424 115
DraIII CAC_nnn'GTG
Cuts at: 0 1128 1338
Size: 1128 210
EaeI y'GGCC_r
Cuts at: 0 26 38 254 1338
Size: 26 12 216 1084
Fragments arranged by size:
1084 216 26 12
EagI C'GGCC_G
Cuts at: 0 254 1338
Size: 254 1084
EarI CTCTTCn'nnn_
Cuts at: 0 1210 1216 1338
Size: 1210 6 122
Fragments arranged by size:
1210 122 6
EcoICRI GAG'CTC
Cuts at: 0 1203 1338
Size: 1203 135
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 1075 1221 1338
Size: 505 570 146 117
Fragments arranged by size:
570 505 146 117
EcoNI CCTnn'n_nnAGG
Cuts at: 0 472 661 1338
Size: 472 189 677
Fragments arranged by size:
677 472 189
EcoO109I rG'GnC_Cy
Cuts at: 0 106 564 633 795 1338
Size: 106 458 69 162 543
Fragments arranged by size:
543 458 162 106 69
HaeII r_GCGC'y
Cuts at: 0 78 189 271 426 553 834 942 1338
Size: 78 111 82 155 127 281 108 396
Fragments arranged by size:
396 281 155 127 111 108 82 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 739 771 801 833 1338
Size: 739 32 30 32 505
Fragments arranged by size:
739 505 32 32 30
KasI G'GCGC_C
Cuts at: 0 74 185 422 1338
Size: 74 111 237 916
Fragments arranged by size:
916 237 111 74
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 1338
Size: 864 474
MscI TGG'CCA
Cuts at: 0 28 40 1338
Size: 28 12 1298
Fragments arranged by size:
1298 28 12
MslI CAynn'nnrTG
Cuts at: 0 34 739 821 919 1280 1338
Size: 34 705 82 98 361 58
Fragments arranged by size:
705 361 98 82 58 34
MspA1I CmG'CkG
Cuts at: 0 216 597 1338
Size: 216 381 741
Fragments arranged by size:
741 381 216
NarI GG'CG_CC
Cuts at: 0 75 186 423 1338
Size: 75 111 237 915
Fragments arranged by size:
915 237 111 75
NcoI C'CATG_G
Cuts at: 0 1278 1338
Size: 1278 60
NdeI CA'TA_TG
Cuts at: 0 974 1338
Size: 974 364
NspI r_CATG'y
Cuts at: 0 145 906 1175 1338
Size: 145 761 269 163
Fragments arranged by size:
761 269 163 145
PasI CC'CwG_GG
Cuts at: 0 960 1116 1338
Size: 960 156 222
Fragments arranged by size:
960 222 156
PflMI CCAn_nnn'nTGG
Cuts at: 0 327 1338
Size: 327 1011
PfoI T'CCnGG_A
Cuts at: 0 1106 1338
Size: 1106 232
PpuMI rG'GwC_Cy
Cuts at: 0 564 633 1338
Size: 564 69 705
Fragments arranged by size:
705 564 69
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 148 180 1338
Size: 148 32 1158
Fragments arranged by size:
1158 148 32
PstI C_TGCA'G
Cuts at: 0 215 1232 1338
Size: 215 1017 106
Fragments arranged by size:
1017 215 106
PvuII CAG'CTG
Cuts at: 0 216 597 1338
Size: 216 381 741
Fragments arranged by size:
741 381 216
SacI G_AGCT'C
Cuts at: 0 1205 1338
Size: 1205 133
SanDI GG'GwC_CC
Cuts at: 0 633 1338
Size: 633 705
SapI GCTCTTCn'nnn_
Cuts at: 0 1210 1338
Size: 1210 128
SfcI C'TryA_G
Cuts at: 0 211 1228 1338
Size: 211 1017 110
Fragments arranged by size:
1017 211 110
SfoI GGC'GCC
Cuts at: 0 76 187 424 1338
Size: 76 111 237 914
Fragments arranged by size:
914 237 111 76
SgrAI Cr'CCGG_yG
Cuts at: 0 71 228 1338
Size: 71 157 1110
Fragments arranged by size:
1110 157 71
SmaI CCC'GGG
Cuts at: 0 123 1338
Size: 123 1215
SphI G_CATG'C
Cuts at: 0 1175 1338
Size: 1175 163
SrfI GCCC'GGGC
Cuts at: 0 123 1338
Size: 123 1215
StuI AGG'CCT
Cuts at: 0 667 1141 1338
Size: 667 474 197
Fragments arranged by size:
667 474 197
StyI C'CwwG_G
Cuts at: 0 348 692 1278 1338
Size: 348 344 586 60
Fragments arranged by size:
586 348 344 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 877 952 1338
Size: 334 543 75 386
Fragments arranged by size:
543 386 334 75
TatI w'GTAC_w
Cuts at: 0 996 1338
Size: 996 342
Tth111I GACn'n_nGTC
Cuts at: 0 952 1338
Size: 952 386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 1338
Size: 375 963
XmaI C'CCGG_G
Cuts at: 0 121 1338
Size: 121 1217
XmnI GAAnn'nnTTC
Cuts at: 0 1081 1338
Size: 1081 257
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI AvaI
BanI BanII BbeI BcgI BglI Bme1580I BmgBI Bpu10I
BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI
MscI MslI MspA1I NarI NcoI NdeI NspI PasI
PflMI PfoI PpuMI PsrI PstI PvuII SacI SanDI
SapI SfcI SfoI SgrAI SmaI SphI SrfI StuI
StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI BpmI BpuEI BsaAI BsaBI BsaXI
BsiWI BsmBI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NaeI NgoMIV NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SspI
SwaI TstI XbaI XhoI ZraI