(Linear) (Six-base) MAPSORT of: KIBB4538.seq  Check: 6875  from: 1  to: 1191



KIBB4538.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AatII G_ACGT'C

Cuts at:      0      15    1191 
   Size:         15    1176
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     880    1003    1041    1191 
   Size:        685     195     123      38     150

  Fragments arranged by size: 

                 685     195     150     123      38
 
AleI CACnn'nnGTG

Cuts at:      0     310    1191 
   Size:        310     881
 
AlwNI CAG_nnn'CTG

Cuts at:      0     712    1191 
   Size:        712     479
 
ApoI r'AATT_y

Cuts at:      0     595    1191 
   Size:        595     596
 
AvaI C'yCGr_G

Cuts at:      0     163    1191 
   Size:        163    1028
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      48     528    1191 
   Size:         48     480     663

  Fragments arranged by size: 

                 663     480      48
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      46    1191 
   Size:         46    1145
 
BglII A'GATC_T

Cuts at:      0     804    1110    1191 
   Size:        804     306      81

  Fragments arranged by size: 

                 804     306      81
 
BmgBI CAC'GTC

Cuts at:      0     308    1191 
   Size:        308     883
 
Bpu10I CC'TnA_GC

Cuts at:      0     220    1191 
   Size:        220     971
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1124    1191 
   Size:       1124      67
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1166    1191 
   Size:       1166      25
 
BsaHI Gr'CG_yC

Cuts at:      0      12    1191 
   Size:         12    1179
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1084    1114    1191 
   Size:       1084      30      77

  Fragments arranged by size: 

                1084      77      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1090    1191 
   Size:       1090     101
 
BseYI C'CCAG_C

Cuts at:      0     543    1191 
   Size:        543     648
 
BsiHKAI G_wGCw'C

Cuts at:      0    1179    1191 
   Size:       1179      12
 
Bsp1286I G_dGCh'C

Cuts at:      0    1179    1191 
   Size:       1179      12
 
BsrFI r'CCGG_y

Cuts at:      0     200    1191 
   Size:        200     991
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     835    1191 
   Size:        835     356
 
BstYI r'GATC_y

Cuts at:      0     804    1110    1191 
   Size:        804     306      81

  Fragments arranged by size: 

                 804     306      81
 
Bsu36I CC'TnA_GG

Cuts at:      0     298     949    1191 
   Size:        298     651     242

  Fragments arranged by size: 

                 651     298     242
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     746    1191 
   Size:        746     445
 
BtsI GCAGTG_nn'

Cuts at:      0     385    1191 
   Size:        385     806
 
DraI TTT'AAA

Cuts at:      0     348    1184    1191 
   Size:        348     836       7

  Fragments arranged by size: 

                 836     348       7
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     301    1191 
   Size:        301     890
 
EaeI y'GGCC_r

Cuts at:      0     936    1191 
   Size:        936     255
 
EarI CTCTTCn'nnn_

Cuts at:      0     814    1191 
   Size:        814     377
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     685     880    1003    1041    1191 
   Size:        685     195     123      38     150

  Fragments arranged by size: 

                 685     195     150     123      38
 
EcoRV GAT'ATC

Cuts at:      0     152    1071    1191 
   Size:        152     919     120

  Fragments arranged by size: 

                 919     152     120
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     640    1191 
   Size:        640     551
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1144    1176    1191 
   Size:       1144      32      15

  Fragments arranged by size: 

                1144      32      15
 
MslI CAynn'nnrTG

Cuts at:      0     310     625    1191 
   Size:        310     315     566

  Fragments arranged by size: 

                 566     315     310
 
NspI r_CATG'y

Cuts at:      0     228    1191 
   Size:        228     963
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     525    1191 
   Size:        525     666
 
PfoI T'CCnGG_A

Cuts at:      0     482    1191 
   Size:        482     709
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     190     222    1191 
   Size:        190      32     969

  Fragments arranged by size: 

                 969     190      32
 
ScaI AGT'ACT

Cuts at:      0     696     865    1191 
   Size:        696     169     326

  Fragments arranged by size: 

                 696     326     169
 
SexAI A'CCwGG_T

Cuts at:      0     971    1191 
   Size:        971     220
 
SfcI C'TryA_G

Cuts at:      0     636    1191 
   Size:        636     555
 
SmlI C'TyrA_G

Cuts at:      0    1139    1191 
   Size:       1139      52
 
SphI G_CATG'C

Cuts at:      0     228    1191 
   Size:        228     963
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     769    1191 
   Size:        769     422
 
TatI w'GTAC_w

Cuts at:      0     206     694     863    1122    1191 
   Size:        206     488     169     259      69

  Fragments arranged by size: 

                 488     259     206     169      69
 
Tth111I GACn'n_nGTC

Cuts at:      0     973    1191 
   Size:        973     218
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     103    1191 
   Size:        103    1088
 
XmnI GAAnn'nnTTC

Cuts at:      0     424    1191 
   Size:        424     767
 
ZraI GAC'GTC

Cuts at:      0      13    1191 
   Size:         13    1178
 

 Enzymes that do cut:

    AatII     AcuI     AleI    AlwNI     ApoI     AvaI     BbsI    BciVI
    BglII    BmgBI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI
    BseYI  BsiHKAI Bsp1286I    BsrFI    BstXI    BstYI   Bsu36I    BtgZI
     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI    EcoRV     FalI
    Hin4I     MslI     NspI    PflMI     PfoI     PsrI     ScaI    SexAI
     SfcI     SmlI     SphI    TaqII     TatI  Tth111I     XcmI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI
     BclI    BfrBI     BglI     BlpI Bme1580I     BmrI     BmtI     BplI
     BpmI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I     BtgI     ClaI    CspCI
   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SspI     StuI     StyI     SwaI     TstI
     XbaI     XhoI     XmaI