Restriction map for
pF1KB4546
(Linear) (Six-base) MAPSORT of: KIBB4546.seq Check: 2063 from: 1 to: 10380
KIBB4546.orf
With 182 enzymes: SgfI *
February 2, 2017 14:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 413 453 3134 3506 4719 10380
Size: 413 40 2681 372 1213 5661
Fragments arranged by size:
5661 2681 1213 413 372 40
AccI GT'mk_AC
Cuts at: 0 1664 1868 5189 6377 10380
Size: 1664 204 3321 1188 4003
Fragments arranged by size:
4003 3321 1664 1188 204
Acc65I G'GTAC_C
Cuts at: 0 3158 6320 10380
Size: 3158 3162 4060
Fragments arranged by size:
4060 3162 3158
AclI AA'CG_TT
Cuts at: 0 6077 10380
Size: 6077 4303
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1576 2216 3309 3448 3510 3946 4532 5010
Size: 1576 640 1093 139 62 436 586 478
Cuts at: 5010 5474 5990 6766 8004 8099 8270 8683 8695
Size: 464 516 776 1238 95 171 413 12
Cuts at: 8695 9047 10380
Size: 352 1333
Fragments arranged by size:
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478 464 436 413 352 171 139 95
62 12
AfeI AGC'GCT
Cuts at: 0 41 8050 10380
Size: 41 8009 2330
Fragments arranged by size:
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AflII C'TTAA_G
Cuts at: 0 8598 10380
Size: 8598 1782
AflIII A'CryG_T
Cuts at: 0 1982 3104 7448 8093 9671 10380
Size: 1982 1122 4344 645 1578 709
Fragments arranged by size:
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AgeI A'CCGG_T
Cuts at: 0 1405 9727 10380
Size: 1405 8322 653
Fragments arranged by size:
8322 1405 653
AhdI GACnn_n'nnGTC
Cuts at: 0 4653 10380
Size: 4653 5727
AleI CACnn'nnGTG
Cuts at: 0 6296 10380
Size: 6296 4084
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 728 7045 10380
Size: 728 6317 3335
Fragments arranged by size:
6317 3335 728
AlwNI CAG_nnn'CTG
Cuts at: 0 14 784 2646 3187 3565 4244 5777 6914
Size: 14 770 1862 541 378 679 1533 1137
Cuts at: 6914 7987 10380
Size: 1073 2393
Fragments arranged by size:
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378 14
ApaI G_GGCC'C
Cuts at: 0 21 5894 8560 10380
Size: 21 5873 2666 1820
Fragments arranged by size:
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ApaLI G'TGCA_C
Cuts at: 0 117 562 2049 6274 10380
Size: 117 445 1487 4225 4106
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 465 1267 1445 1461 1609 2026 2507 2926
Size: 465 802 178 16 148 417 481 419
Cuts at: 2926 4825 5430 5689 6124 8318 8756 10311 10380
Size: 1899 605 259 435 2194 438 1555 69
Fragments arranged by size:
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435 419 417 259 178 148 69 16
AseI AT'TA_AT
Cuts at: 0 6550 10380
Size: 6550 3830
AvaI C'yCGr_G
Cuts at: 0 3869 7528 9326 9974 10380
Size: 3869 3659 1798 648 406
Fragments arranged by size:
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BamHI G'GATC_C
Cuts at: 0 4163 6108 7283 8791 9623 10380
Size: 4163 1945 1175 1508 832 757
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 2879 3158 3494 4399 5300 5675 5964 6152
Size: 2879 279 336 905 901 375 289 188
Cuts at: 6152 6320 7021 7220 7745 9730 10380
Size: 168 701 199 525 1985 650
Fragments arranged by size:
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336 289 279 199 188 168
BanII G_rGCy'C
Cuts at: 0 21 546 906 2361 2462 4070 5894 7575
Size: 21 525 360 1455 101 1608 1824 1681
Cuts at: 7575 8560 10334 10380
Size: 985 1774 46
Fragments arranged by size:
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101 46 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 1347 4711 5906 6769 7056 8111 8671 10359
Size: 1347 3364 1195 863 287 1055 560 1688
Cuts at: 10359 10380
Size: 21
Fragments arranged by size:
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21
BbvCI CC'TCA_GC
Cuts at: 0 61 10380
Size: 61 10319
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4618 4652 8755 8789 10380
Size: 4618 34 4103 34 1591
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 1521 3360 4613 4899 5868 8620 9343 9709
Size: 1521 1839 1253 286 969 2752 723 366
Cuts at: 9709 10380
Size: 671
Fragments arranged by size:
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286
BclI T'GATC_A
Cuts at: 0 1046 2364 9077 10245 10380
Size: 1046 1318 6713 1168 135
Fragments arranged by size:
6713 1318 1168 1046 135
BfrBI ATG'CAT
Cuts at: 0 705 7472 10380
Size: 705 6767 2908
Fragments arranged by size:
6767 2908 705
BglI GCCn_nnn'nGGC
Cuts at: 0 1027 4765 10380
Size: 1027 3738 5615
Fragments arranged by size:
5615 3738 1027
BglII A'GATC_T
Cuts at: 0 1227 1832 5556 5626 7203 8273 10380
Size: 1227 605 3724 70 1577 1070 2107
Fragments arranged by size:
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BlpI GC'TnA_GC
Cuts at: 0 4372 10380
Size: 4372 6008
Bme1580I G_kGCm'C
Cuts at: 0 21 121 454 566 2053 3100 4404 5305
Size: 21 100 333 112 1487 1047 1304 901
Cuts at: 5305 5894 5967 6278 6902 8560 9126 9713 10380
Size: 589 73 311 624 1658 566 587 667
Fragments arranged by size:
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587 566 333 311 112 100 73 21
BmgBI CAC'GTC
Cuts at: 0 6192 10380
Size: 6192 4188
BmrI ACTGGGnnnn_n'
Cuts at: 0 1090 2335 3679 4232 4755 6384 6703 7965
Size: 1090 1245 1344 553 523 1629 319 1262
Cuts at: 7965 8026 8865 9465 9791 9855 10380
Size: 61 839 600 326 64 525
Fragments arranged by size:
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525 523 326 319 64 61
BmtI G_CTAG'C
Cuts at: 0 3505 10380
Size: 3505 6875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4194 10380
Size: 4194 6186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1974 2293 2449 2869 3051 3571 3935
Size: 701 1273 319 156 420 182 520 364
Cuts at: 3935 4035 5035 6279 6853 9237 10201 10380
Size: 100 1000 1244 574 2384 964 179
Fragments arranged by size:
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420 364 319 182 179 156 100
Bpu10I CC'TnA_GC
Cuts at: 0 61 5316 10380
Size: 61 5255 5064
Fragments arranged by size:
5255 5064 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2712 3038 9540 10380
Size: 2712 326 6502 840
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 4136 4948 6641 7525 9102 9844 10380
Size: 4136 812 1693 884 1577 742 536
Fragments arranged by size:
4136 1693 1577 884 812 742 536
BsaAI yAC'GTr
Cuts at: 0 396 2351 7092 9672 10380
Size: 396 1955 4741 2580 708
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 1226 3851 8825 10380
Size: 1226 2625 4974 1555
Fragments arranged by size:
4974 2625 1555 1226
BsaHI Gr'CG_yC
Cuts at: 0 953 2262 3455 7639 10380
Size: 953 1309 1193 4184 2741
Fragments arranged by size:
4184 2741 1309 1193 953
BsaWI w'CCGG_w
Cuts at: 0 1405 8490 9727 10380
Size: 1405 7085 1237 653
Fragments arranged by size:
7085 1405 1237 653
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 604 634 1121 1151 1835 1865 3257 3287
Size: 604 30 487 30 684 30 1392 30
Cuts at: 3287 4865 4895 5196 5226 6722 6752 8035 8065
Size: 1578 30 301 30 1496 30 1283 30
Cuts at: 8065 9580 9610 10380
Size: 1515 30 770
Fragments arranged by size:
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487 301 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1863 2151 2988 3463 6276 6412 9312 9588
Size: 1863 288 837 475 2813 136 2900 276
Cuts at: 9588 10380
Size: 792
Fragments arranged by size:
2900 2813 1863 837 792 475 288 276
136
BseYI C'CCAG_C
Cuts at: 0 14 48 521 838 1025 2091 3233 3334
Size: 14 34 473 317 187 1066 1142 101
Cuts at: 3334 6223 8039 9163 9656 10124 10380
Size: 2889 1816 1124 493 468 256
Fragments arranged by size:
2889 1816 1142 1124 1066 493 473 468
317 256 187 101 34 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 2373 3129 4289 5486 6274 6697 7473
Size: 396 1977 756 1160 1197 788 423 776
Cuts at: 7473 8347 8759 9705 10380
Size: 874 412 946 675
Fragments arranged by size:
1977 1197 1160 946 874 788 776 756
675 423 412 396
BsiEI CG_ry'CG
Cuts at: 0 9728 10380
Size: 9728 652
BsiHKAI G_wGCw'C
Cuts at: 0 121 167 546 566 2053 2350 5317 6218
Size: 121 46 379 20 1487 297 2967 901
Cuts at: 6218 6278 6740 7575 8129 9117 10380
Size: 60 462 835 554 988 1263
Fragments arranged by size:
2967 1487 1263 988 901 835 554 462
379 297 121 60 46 20
BsmI GAATG_Cn'
Cuts at: 0 1825 2902 3178 4752 6015 7264 10380
Size: 1825 1077 276 1574 1263 1249 3116
Fragments arranged by size:
3116 1825 1574 1263 1249 1077 276
Bsp1286I G_dGCh'C
Cuts at: 0 21 121 167 454 546 566 906 2053
Size: 21 100 46 287 92 20 340 1147
Cuts at: 2053 2350 2361 2462 3100 4070 4404 5305 5317
Size: 297 11 101 638 970 334 901 12
Cuts at: 5317 5894 5967 6218 6278 6740 6902 7575 8129
Size: 577 73 251 60 462 162 673 554
Cuts at: 8129 8560 9117 9126 9713 10334 10380
Size: 431 557 9 587 621 46
Fragments arranged by size:
1147 970 901 673 638 621 587 577
557 554 462 431 340 334 297 287
251 162 101 100 92 73 60 46
46 21 20 12 11 9
BspHI T'CATG_A
Cuts at: 0 1467 2454 2579 3972 6686 8202 10242 10380
Size: 1467 987 125 1393 2714 1516 2040 138
Fragments arranged by size:
2714 2040 1516 1467 1393 987 138 125
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 453 3134 3506 4567 4697 4719 6809
Size: 413 40 2681 372 1061 130 22 2090
Cuts at: 6809 7829 10380
Size: 1020 2551
Fragments arranged by size:
2681 2551 2090 1061 1020 413 372 130
40 22
BsrDI GCAATG_nn'
Cuts at: 0 717 1261 3833 6816 6824 9090 10380
Size: 717 544 2572 2983 8 2266 1290
Fragments arranged by size:
2983 2572 2266 1290 717 544 8
BsrFI r'CCGG_y
Cuts at: 0 1405 6340 8065 9727 10380
Size: 1405 4935 1725 1662 653
Fragments arranged by size:
4935 1725 1662 1405 653
BsrGI T'GTAC_A
Cuts at: 0 4217 5043 5566 8149 9674 10380
Size: 4217 826 523 2583 1525 706
Fragments arranged by size:
4217 2583 1525 826 706 523
BssHII G'CGCG_C
Cuts at: 0 67 69 10380
Size: 67 2 10311
Fragments arranged by size:
10311 67 2
BssSI C'ACGA_G
Cuts at: 0 743 6644 6768 8044 10380
Size: 743 5901 124 1276 2336
Fragments arranged by size:
5901 2336 1276 743 124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3173 4037 4559 6159 9503 10380
Size: 3173 864 522 1600 3344 877
Fragments arranged by size:
3344 3173 1600 877 864 522
BstBI TT'CG_AA
Cuts at: 0 6518 10380
Size: 6518 3862
BstEII G'GTnAC_C
Cuts at: 0 361 9400 10380
Size: 361 9039 980
Fragments arranged by size:
9039 980 361
BstXI CCAn_nnnn'nTGG
Cuts at: 0 358 653 1731 2628 6359 6813 7663 9171
Size: 358 295 1078 897 3731 454 850 1508
Cuts at: 9171 9963 10380
Size: 792 417
Fragments arranged by size:
3731 1508 1078 897 850 792 454 417
358 295
BstYI r'GATC_y
Cuts at: 0 1227 1832 4163 5556 5626 6108 6301 7203
Size: 1227 605 2331 1393 70 482 193 902
Cuts at: 7203 7283 7695 8273 8791 8826 9623 9966 10380
Size: 80 412 578 518 35 797 343 414
Fragments arranged by size:
2331 1393 1227 902 797 605 578 518
482 414 412 343 193 80 70 35
Bsu36I CC'TnA_GG
Cuts at: 0 947 1420 5295 10380
Size: 947 473 3875 5085
Fragments arranged by size:
5085 3875 947 473
BtgI C'CryG_G
Cuts at: 0 126 1179 1394 1788 2187 2865 4254 6104
Size: 126 1053 215 394 399 678 1389 1850
Cuts at: 6104 7461 7634 8586 9906 10380
Size: 1357 173 952 1320 474
Fragments arranged by size:
1850 1389 1357 1320 1053 952 678 474
399 394 215 173 126
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 10104 10380
Size: 10104 276
BtsI GCAGTG_nn'
Cuts at: 0 15 384 3100 3117 4254 5744 7461 8231
Size: 15 369 2716 17 1137 1490 1717 770
Cuts at: 8231 8536 8771 10079 10380
Size: 305 235 1308 301
Fragments arranged by size:
2716 1717 1490 1308 1137 770 369 305
301 235 17 15
ClaI AT'CG_AT
Cuts at: 0 3855 5837 6923 8540 10380
Size: 3855 1982 1086 1617 1840
Fragments arranged by size:
3855 1982 1840 1617 1086
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 961 996 3043 3078 10380
Size: 961 35 2047 35 7302
Fragments arranged by size:
7302 2047 961 35 35
DraI TTT'AAA
Cuts at: 0 5523 9366 10380
Size: 5523 3843 1014
Fragments arranged by size:
5523 3843 1014
DraIII CAC_nnn'GTG
Cuts at: 0 8750 10062 10380
Size: 8750 1312 318
Fragments arranged by size:
8750 1312 318
EaeI y'GGCC_r
Cuts at: 0 2222 2682 2808 4251 7458 9283 10380
Size: 2222 460 126 1443 3207 1825 1097
Fragments arranged by size:
3207 2222 1825 1443 1097 460 126
EarI CTCTTCn'nnn_
Cuts at: 0 3309 5090 7544 9492 10380
Size: 3309 1781 2454 1948 888
Fragments arranged by size:
3309 2454 1948 1781 888
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3425 10380
Size: 3425 6955
EcoICRI GAG'CTC
Cuts at: 0 544 7573 10380
Size: 544 7029 2807
Fragments arranged by size:
7029 2807 544
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1576 1974 2216 2293 2449 2869 3051
Size: 701 875 398 242 77 156 420 182
Cuts at: 3051 3309 3448 3510 3571 3935 3946 4035 4532
Size: 258 139 62 61 364 11 89 497
Cuts at: 4532 5010 5035 5474 5990 6279 6766 6853 8004
Size: 478 25 439 516 289 487 87 1151
Cuts at: 8004 8099 8270 8683 8695 9047 9237 10201 10380
Size: 95 171 413 12 352 190 964 179
Fragments arranged by size:
1151 964 875 701 516 497 487 478
439 420 413 398 364 352 289 258
242 190 182 179 171 156 139 95
89 87 77 62 61 25 12 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 946 2375 4693 10380
Size: 946 1429 2318 5687
Fragments arranged by size:
5687 2318 1429 946
EcoO109I rG'GnC_Cy
Cuts at: 0 2235 3360 5219 7349 8557 10380
Size: 2235 1125 1859 2130 1208 1823
Fragments arranged by size:
2235 2130 1859 1823 1208 1125
EcoRI G'AATT_C
Cuts at: 0 2507 8756 10380
Size: 2507 6249 1624
Fragments arranged by size:
6249 2507 1624
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2917 3208 4562 8700 10380
Size: 2917 291 1354 4138 1680
Fragments arranged by size:
4138 2917 1680 1354 291
FspI TGC'GCA
Cuts at: 0 6631 10380
Size: 6631 3749
HaeII r_GCGC'y
Cuts at: 0 43 1797 8030 8052 10380
Size: 43 1754 6233 22 2328
Fragments arranged by size:
6233 2328 1754 43 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 323 355 604 636 1150 1182 1243 1275
Size: 323 32 249 32 514 32 61 32
Cuts at: 1275 1280 1312 1835 1867 2092 2124 4426 4458
Size: 5 32 523 32 225 32 2302 32
Cuts at: 4458 4473 4505 4639 4671 4882 4914 5463 5495
Size: 15 32 134 32 211 32 549 32
Cuts at: 5495 5759 5791 6085 6117 7670 7702 7890 7922
Size: 264 32 294 32 1553 32 188 32
Cuts at: 7922 8643 8675 9140 9172 9427 9459 9580 9612
Size: 721 32 465 32 255 32 121 32
Cuts at: 9612 9757 9789 10380
Size: 145 32 591
Fragments arranged by size:
2302 1553 721 591 549 523 514 465
323 294 264 255 249 225 211 188
145 134 121 61 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 15 5
HincII GTy'rAC
Cuts at: 0 6730 9549 10095 10380
Size: 6730 2819 546 285
Fragments arranged by size:
6730 2819 546 285
HindIII A'AGCT_T
Cuts at: 0 2139 8475 10380
Size: 2139 6336 1905
Fragments arranged by size:
6336 2139 1905
KpnI G_GTAC'C
Cuts at: 0 3162 6324 10380
Size: 3162 3162 4056
Fragments arranged by size:
4056 3162 3162
MfeI C'AATT_G
Cuts at: 0 1991 5904 9169 9662 10380
Size: 1991 3913 3265 493 718
Fragments arranged by size:
3913 3265 1991 718 493
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 3721 4389 4625 5134 6493 7567 8262
Size: 571 3150 668 236 509 1359 1074 695
Cuts at: 8262 8335 8458 8909 10380
Size: 73 123 451 1471
Fragments arranged by size:
3150 1471 1359 1074 695 668 571 509
451 236 123 73
MscI TGG'CCA
Cuts at: 0 2224 2684 2810 4253 7460 9285 10380
Size: 2224 460 126 1443 3207 1825 1095
Fragments arranged by size:
3207 2224 1825 1443 1095 460 126
MslI CAynn'nnrTG
Cuts at: 0 710 847 1184 1472 2954 3205 4725 6296
Size: 710 137 337 288 1482 251 1520 1571
Cuts at: 6296 6637 7027 7447 7661 8098 10380
Size: 341 390 420 214 437 2282
Fragments arranged by size:
2282 1571 1520 1482 710 437 420 390
341 337 288 251 214 137
MspA1I CmG'CkG
Cuts at: 0 3029 3348 3562 3593 6106 7916 9582 9620
Size: 3029 319 214 31 2513 1810 1666 38
Cuts at: 9620 9707 9891 10031 10380
Size: 87 184 140 349
Fragments arranged by size:
3029 2513 1810 1666 349 319 214 184
140 87 38 31
NaeI GCC'GGC
Cuts at: 0 6342 8067 10380
Size: 6342 1725 2313
Fragments arranged by size:
6342 2313 1725
NcoI C'CATG_G
Cuts at: 0 1179 1394 1788 4254 7461 8586 9906 10380
Size: 1179 215 394 2466 3207 1125 1320 474
Fragments arranged by size:
3207 2466 1320 1179 1125 474 394 215
NdeI CA'TA_TG
Cuts at: 0 735 3139 10380
Size: 735 2404 7241
Fragments arranged by size:
7241 2404 735
NgoMIV G'CCGG_C
Cuts at: 0 6340 8065 10380
Size: 6340 1725 2315
Fragments arranged by size:
6340 2315 1725
NheI G'CTAG_C
Cuts at: 0 3501 10380
Size: 3501 6879
NsiI A_TGCA'T
Cuts at: 0 707 7474 10380
Size: 707 6767 2906
Fragments arranged by size:
6767 2906 707
NspI r_CATG'y
Cuts at: 0 1986 3108 6636 6667 7452 8097 8529 10017
Size: 1986 1122 3528 31 785 645 432 1488
Cuts at: 10017 10117 10380
Size: 100 263
Fragments arranged by size:
3528 1986 1488 1122 785 645 432 263
100 31
PasI CC'CwG_GG
Cuts at: 0 4462 6914 10380
Size: 4462 2452 3466
Fragments arranged by size:
4462 3466 2452
PciI A'CATG_T
Cuts at: 0 1982 3104 7448 8093 10380
Size: 1982 1122 4344 645 2287
Fragments arranged by size:
4344 2287 1982 1122 645
PflMI CCAn_nnn'nTGG
Cuts at: 0 1975 2680 5116 8976 9174 10062 10380
Size: 1975 705 2436 3860 198 888 318
Fragments arranged by size:
3860 2436 1975 888 705 318 198
PfoI T'CCnGG_A
Cuts at: 0 207 2426 4166 4661 8622 10380
Size: 207 2219 1740 495 3961 1758
Fragments arranged by size:
3961 2219 1758 1740 495 207
PmlI CAC'GTG
Cuts at: 0 396 2351 9672 10380
Size: 396 1955 7321 708
Fragments arranged by size:
7321 1955 708 396
PpuMI rG'GwC_Cy
Cuts at: 0 3360 5219 7349 10380
Size: 3360 1859 2130 3031
Fragments arranged by size:
3360 3031 2130 1859
PshAI GACnn'nnGTC
Cuts at: 0 10359 10380
Size: 10359 21
PsiI TTA'TAA
Cuts at: 0 5789 10380
Size: 5789 4591
PspOMI G'GGCC_C
Cuts at: 0 17 5890 8556 10380
Size: 17 5873 2666 1824
Fragments arranged by size:
5873 2666 1824 17
PspXI vC'TCGA_Gb
Cuts at: 0 9974 10380
Size: 9974 406
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 9323 9355 10380
Size: 9323 32 1025
Fragments arranged by size:
9323 1025 32
PstI C_TGCA'G
Cuts at: 0 4029 4249 8061 8581 9706 10380
Size: 4029 220 3812 520 1125 674
Fragments arranged by size:
4029 3812 1125 674 520 220
PvuII CAG'CTG
Cuts at: 0 3348 3562 9582 9891 10031 10380
Size: 3348 214 6020 309 140 349
Fragments arranged by size:
6020 3348 349 309 214 140
SacI G_AGCT'C
Cuts at: 0 546 7575 10380
Size: 546 7029 2805
Fragments arranged by size:
7029 2805 546
SacII CC_GC'GG
Cuts at: 0 6107 10380
Size: 6107 4273
ScaI AGT'ACT
Cuts at: 0 2854 3945 3967 8518 8739 10380
Size: 2854 1091 22 4551 221 1641
Fragments arranged by size:
4551 2854 1641 1091 221 22
SexAI A'CCwGG_T
Cuts at: 0 2484 2837 3583 7345 10380
Size: 2484 353 746 3762 3035
Fragments arranged by size:
3762 3035 2484 746 353
SfcI C'TryA_G
Cuts at: 0 476 1133 1283 1661 1884 3901 4025 4245
Size: 476 657 150 378 223 2017 124 220
Cuts at: 4245 4838 5013 6742 6906 7132 8057 8577 9702
Size: 593 175 1729 164 226 925 520 1125
Cuts at: 9702 10380
Size: 678
Fragments arranged by size:
2017 1729 1125 925 678 657 593 520
476 378 226 223 220 175 164 150
124
SmlI C'TyrA_G
Cuts at: 0 2727 3053 3869 8598 9555 9974 10380
Size: 2727 326 816 4729 957 419 406
Fragments arranged by size:
4729 2727 957 816 419 406 326
SspI AAT'ATT
Cuts at: 0 6948 7792 10380
Size: 6948 844 2588
Fragments arranged by size:
6948 2588 844
StuI AGG'CCT
Cuts at: 0 764 10380
Size: 764 9616
StyI C'CwwG_G
Cuts at: 0 1179 1394 1788 1974 2658 3162 4126 4254
Size: 1179 215 394 186 684 504 964 128
Cuts at: 4254 4869 5305 7461 7773 8586 9759 9906 10380
Size: 615 436 2156 312 813 1173 147 474
Fragments arranged by size:
2156 1179 1173 964 813 684 615 504
474 436 394 312 215 186 147 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 136 581 1880 3194 3366 5923 6373 7028
Size: 136 445 1299 1314 172 2557 450 655
Cuts at: 7028 7238 7733 10380
Size: 210 495 2647
Fragments arranged by size:
2647 2557 1314 1299 655 495 450 445
210 172 136
TatI w'GTAC_w
Cuts at: 0 2852 3320 3467 3512 3943 3965 4217 5043
Size: 2852 468 147 45 431 22 252 826
Cuts at: 5043 5286 5566 7514 7618 7847 8149 8516 8737
Size: 243 280 1948 104 229 302 367 221
Cuts at: 8737 9674 9920 10380
Size: 937 246 460
Fragments arranged by size:
2852 1948 937 826 468 460 431 367
302 280 252 246 243 229 221 147
104 45 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4304 4336 5196 5228 5333 5365 6477 6509
Size: 4304 32 860 32 105 32 1112 32
Cuts at: 6509 8035 8067 8201 8233 8276 8308 8783 8815
Size: 1526 32 134 32 43 32 475 32
Cuts at: 8815 10380
Size: 1565
Fragments arranged by size:
4304 1565 1526 1112 860 475 134 105
43 32 32 32 32 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3385 7969 9592 10380
Size: 3385 4584 1623 788
Fragments arranged by size:
4584 3385 1623 788
XbaI T'CTAG_A
Cuts at: 0 5559 10380
Size: 5559 4821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 799 4411 5250 8441 9171 9676 10380
Size: 799 3612 839 3191 730 505 704
Fragments arranged by size:
3612 3191 839 799 730 704 505
XhoI C'TCGA_G
Cuts at: 0 3869 9974 10380
Size: 3869 6105 406
Fragments arranged by size:
6105 3869 406
XmnI GAAnn'nnTTC
Cuts at: 0 995 1244 2900 6588 6948 8409 8668 10380
Size: 995 249 1656 3688 360 1461 259 1712
Fragments arranged by size:
3688 1712 1656 1461 995 360 259 249
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KpnI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuII SacI
SacII ScaI SexAI SfcI SmlI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AloI AscI AsiSI AvrII BaeI BbeI BsiWI
BsmBI BspEI BsrBI BstZ17I DrdI EagI EcoRV FseI
FspAI HpaI KasI MluI NarI NotI NruI PacI
PmeI PpiI PvuI RsrII SalI SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI XmaI ZraI