Restriction map for
pF1KB4563
(Linear) (Six-base) MAPSORT of: KIBB4563.seq Check: 9208 from: 1 to: 2730
KIBB4563.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2352 2730
Size: 2352 378
AccI GT'mk_AC
Cuts at: 0 532 2730
Size: 532 2198
Acc65I G'GTAC_C
Cuts at: 0 823 2234 2332 2730
Size: 823 1411 98 398
Fragments arranged by size:
1411 823 398 98
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 886 1546 1587 1601 1713 2334 2730
Size: 754 132 660 41 14 112 621 396
Fragments arranged by size:
754 660 621 396 132 112 41 14
AflIII A'CryG_T
Cuts at: 0 1193 2277 2730
Size: 1193 1084 453
Fragments arranged by size:
1193 1084 453
AhdI GACnn_n'nnGTC
Cuts at: 0 43 2730
Size: 43 2687
AleI CACnn'nnGTG
Cuts at: 0 2359 2498 2730
Size: 2359 139 232
Fragments arranged by size:
2359 232 139
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1046 1492 2377 2730
Size: 1046 446 885 353
Fragments arranged by size:
1046 885 446 353
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2593 2625 2730
Size: 2593 32 105
Fragments arranged by size:
2593 105 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 1297 1551 2478 2730
Size: 1050 247 254 927 252
Fragments arranged by size:
1050 927 254 252 247
ApaI G_GGCC'C
Cuts at: 0 630 1977 2730
Size: 630 1347 753
Fragments arranged by size:
1347 753 630
ApoI r'AATT_y
Cuts at: 0 745 1210 2215 2713 2730
Size: 745 465 1005 498 17
Fragments arranged by size:
1005 745 498 465 17
AvaI C'yCGr_G
Cuts at: 0 245 914 1205 2243 2357 2730
Size: 245 669 291 1038 114 373
Fragments arranged by size:
1038 669 373 291 245 114
BanI G'GyrC_C
Cuts at: 0 823 1843 2110 2234 2332 2406 2730
Size: 823 1020 267 124 98 74 324
Fragments arranged by size:
1020 823 324 267 124 98 74
BanII G_rGCy'C
Cuts at: 0 230 630 860 1692 1727 1977 2179 2730
Size: 230 400 230 832 35 250 202 551
Fragments arranged by size:
832 551 400 250 230 230 202 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1593 2274 2730
Size: 1593 681 456
Fragments arranged by size:
1593 681 456
BbvCI CC'TCA_GC
Cuts at: 0 151 931 1352 2730
Size: 151 780 421 1378
Fragments arranged by size:
1378 780 421 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1803 1837 2248 2282 2730
Size: 1803 34 411 34 448
Fragments arranged by size:
1803 448 411 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 207 1521 2730
Size: 207 1314 1209
Fragments arranged by size:
1314 1209 207
BclI T'GATC_A
Cuts at: 0 1385 2621 2730
Size: 1385 1236 109
Fragments arranged by size:
1385 1236 109
BglI GCCn_nnn'nGGC
Cuts at: 0 1942 2730
Size: 1942 788
BlpI GC'TnA_GC
Cuts at: 0 393 2730
Size: 393 2337
Bme1580I G_kGCm'C
Cuts at: 0 630 685 1977 2244 2411 2730
Size: 630 55 1292 267 167 319
Fragments arranged by size:
1292 630 319 267 167 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 2109 2730
Size: 2109 621
BmtI G_CTAG'C
Cuts at: 0 2042 2730
Size: 2042 688
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 814 1153 2038 2730
Size: 704 110 339 885 692
Fragments arranged by size:
885 704 692 339 110
Bpu10I CC'TnA_GC
Cuts at: 0 134 151 431 931 1352 2730
Size: 134 17 280 500 421 1378
Fragments arranged by size:
1378 500 421 280 134 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 2357 2730
Size: 511 1846 373
Fragments arranged by size:
1846 511 373
BsaI GGTCTCn'nnnn_
Cuts at: 0 2098 2730
Size: 2098 632
BsaAI yAC'GTr
Cuts at: 0 2091 2730
Size: 2091 639
BsaHI Gr'CG_yC
Cuts at: 0 4 2435 2730
Size: 4 2431 295
Fragments arranged by size:
2431 295 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1864 1894 2593 2623 2730
Size: 1864 30 699 30 107
Fragments arranged by size:
1864 699 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 119 625 850 1700 1954 1999 2014
Size: 18 101 506 225 850 254 45 15
Cuts at: 2014 2035 2730
Size: 21 695
Fragments arranged by size:
850 695 506 254 225 101 45 21
18 15
BseYI C'CCAG_C
Cuts at: 0 1278 2556 2730
Size: 1278 1278 174
Fragments arranged by size:
1278 1278 174
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1732 1816 2065 2113 2730
Size: 76 1656 84 249 48 617
Fragments arranged by size:
1656 617 249 84 76 48
BsiHKAI G_wGCw'C
Cuts at: 0 860 1874 2730
Size: 860 1014 856
Fragments arranged by size:
1014 860 856
BsmI GAATG_Cn'
Cuts at: 0 1059 1681 2643 2730
Size: 1059 622 962 87
Fragments arranged by size:
1059 962 622 87
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1621 2730
Size: 1621 1109
Bsp1286I G_dGCh'C
Cuts at: 0 230 630 685 860 1692 1727 1874 1977
Size: 230 400 55 175 832 35 147 103
Cuts at: 1977 2179 2244 2411 2730
Size: 202 65 167 319
Fragments arranged by size:
832 400 319 230 202 175 167 147
103 65 55 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1035 1561 2087 2352 2730
Size: 1035 526 526 265 378
Fragments arranged by size:
1035 526 526 378 265
BsrBI CCG'CTC
Cuts at: 0 20 138 2730
Size: 20 118 2592
Fragments arranged by size:
2592 118 20
BsrDI GCAATG_nn'
Cuts at: 0 365 1346 2150 2151 2207 2730
Size: 365 981 804 1 56 523
Fragments arranged by size:
981 804 523 365 56 1
BsrFI r'CCGG_y
Cuts at: 0 440 1394 1810 2107 2503 2730
Size: 440 954 416 297 396 227
Fragments arranged by size:
954 440 416 396 297 227
BssSI C'ACGA_G
Cuts at: 0 116 2730
Size: 116 2614
BstAPI GCAn_nnn'nTGC
Cuts at: 0 680 1551 1676 2730
Size: 680 871 125 1054
Fragments arranged by size:
1054 871 680 125
BstBI TT'CG_AA
Cuts at: 0 258 1218 2730
Size: 258 960 1512
Fragments arranged by size:
1512 960 258
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1761 2328 2730
Size: 1761 567 402
Fragments arranged by size:
1761 567 402
BstYI r'GATC_y
Cuts at: 0 522 2730
Size: 522 2208
BstZ17I GTA'TAC
Cuts at: 0 533 2730
Size: 533 2197
BtgI C'CryG_G
Cuts at: 0 41 1994 2378 2438 2730
Size: 41 1953 384 60 292
Fragments arranged by size:
1953 384 292 60 41
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2561 2730
Size: 2561 169
BtsI GCAGTG_nn'
Cuts at: 0 621 717 2730
Size: 621 96 2013
Fragments arranged by size:
2013 621 96
DraIII CAC_nnn'GTG
Cuts at: 0 558 886 2474 2730
Size: 558 328 1588 256
Fragments arranged by size:
1588 558 328 256
EcoICRI GAG'CTC
Cuts at: 0 858 2730
Size: 858 1872
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 704 754 814 886 1153 1546 1587 1601
Size: 704 50 60 72 267 393 41 14
Cuts at: 1601 1713 2038 2334 2730
Size: 112 325 296 396
Fragments arranged by size:
704 396 393 325 296 267 112 72
60 50 41 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 865 2730
Size: 865 1865
EcoO109I rG'GnC_Cy
Cuts at: 0 37 626 627 2310 2730
Size: 37 589 1 1683 420
Fragments arranged by size:
1683 589 420 37 1
EcoRV GAT'ATC
Cuts at: 0 2460 2730
Size: 2460 270
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1762 1908 2730
Size: 1762 146 822
Fragments arranged by size:
1762 822 146
FspI TGC'GCA
Cuts at: 0 2143 2596 2730
Size: 2143 453 134
Fragments arranged by size:
2143 453 134
HaeII r_GCGC'y
Cuts at: 0 2663 2682 2730
Size: 2663 19 48
Fragments arranged by size:
2663 48 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 1399 1431 2730
Size: 29 32 1338 32 1299
Fragments arranged by size:
1338 1299 32 32 29
HindIII A'AGCT_T
Cuts at: 0 1225 2730
Size: 1225 1505
KpnI G_GTAC'C
Cuts at: 0 827 2238 2336 2730
Size: 827 1411 98 394
Fragments arranged by size:
1411 827 394 98
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1143 2099 2730
Size: 1143 956 631
Fragments arranged by size:
1143 956 631
MslI CAynn'nnrTG
Cuts at: 0 73 839 1069 1427 2359 2404 2498 2730
Size: 73 766 230 358 932 45 94 232
Fragments arranged by size:
932 766 358 232 230 94 73 45
MspA1I CmG'CkG
Cuts at: 0 43 60 1566 2730
Size: 43 17 1506 1164
Fragments arranged by size:
1506 1164 43 17
NaeI GCC'GGC
Cuts at: 0 1812 2505 2730
Size: 1812 693 225
Fragments arranged by size:
1812 693 225
NgoMIV G'CCGG_C
Cuts at: 0 1810 2503 2730
Size: 1810 693 227
Fragments arranged by size:
1810 693 227
NheI G'CTAG_C
Cuts at: 0 2038 2730
Size: 2038 692
NruI TCG'CGA
Cuts at: 0 762 2730
Size: 762 1968
NspI r_CATG'y
Cuts at: 0 604 1197 2142 2281 2730
Size: 604 593 945 139 449
Fragments arranged by size:
945 604 593 449 139
PasI CC'CwG_GG
Cuts at: 0 230 312 2730
Size: 230 82 2418
Fragments arranged by size:
2418 230 82
PciI A'CATG_T
Cuts at: 0 1193 2277 2730
Size: 1193 1084 453
Fragments arranged by size:
1193 1084 453
PfoI T'CCnGG_A
Cuts at: 0 518 2730
Size: 518 2212
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2593 2625 2730
Size: 2593 32 105
Fragments arranged by size:
2593 105 32
PpuMI rG'GwC_Cy
Cuts at: 0 37 2310 2730
Size: 37 2273 420
Fragments arranged by size:
2273 420 37
PspOMI G'GGCC_C
Cuts at: 0 626 1973 2730
Size: 626 1347 757
Fragments arranged by size:
1347 757 626
PstI C_TGCA'G
Cuts at: 0 738 1446 1772 2528 2730
Size: 738 708 326 756 202
Fragments arranged by size:
756 738 708 326 202
SacI G_AGCT'C
Cuts at: 0 860 2730
Size: 860 1870
SacII CC_GC'GG
Cuts at: 0 44 2730
Size: 44 2686
SanDI GG'GwC_CC
Cuts at: 0 37 2730
Size: 37 2693
SexAI A'CCwGG_T
Cuts at: 0 650 2730
Size: 650 2080
SfcI C'TryA_G
Cuts at: 0 734 1442 1768 2168 2524 2721 2730
Size: 734 708 326 400 356 197 9
Fragments arranged by size:
734 708 400 356 326 197 9
SmaI CCC'GGG
Cuts at: 0 916 1207 2730
Size: 916 291 1523
Fragments arranged by size:
1523 916 291
SmlI C'TyrA_G
Cuts at: 0 245 526 2372 2730
Size: 245 281 1846 358
Fragments arranged by size:
1846 358 281 245
StyI C'CwwG_G
Cuts at: 0 339 547 1034 1754 1883 2540 2730
Size: 339 208 487 720 129 657 190
Fragments arranged by size:
720 657 487 339 208 190 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1162 2077 2128 2730
Size: 1162 915 51 602
Fragments arranged by size:
1162 915 602 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 500 509 532 1864 1896 2730
Size: 477 23 9 23 1332 32 834
Fragments arranged by size:
1332 834 477 32 23 23 9
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1002 2547 2730
Size: 1002 1545 183
Fragments arranged by size:
1545 1002 183
XhoI C'TCGA_G
Cuts at: 0 245 2730
Size: 245 2485
XmaI C'CCGG_G
Cuts at: 0 914 1205 2730
Size: 914 291 1525
Fragments arranged by size:
1525 914 291
XmnI GAAnn'nnTTC
Cuts at: 0 1214 2608 2730
Size: 1214 1394 122
Fragments arranged by size:
1394 1214 122
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraIII EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI FspI HaeII Hin4I HindIII KpnI
MmeI MslI MspA1I NaeI NgoMIV NheI NruI NspI
PasI PciI PfoI PpiI PpuMI PspOMI PstI SacI
SacII SanDI SexAI SfcI SmaI SmlI StyI TaqII
TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BfrBI BglII BmgBI
BplI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrGI
BssHII BstEII Bsu36I ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoRI FseI FspAI HincII HpaI
KasI MfeI MluI MscI NarI NcoI NdeI NotI
NsiI PacI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI ZraI