Restriction map for
pF1KB4571
(Linear) (Six-base) MAPSORT of: KIBB4571.seq Check: 3818 from: 1 to: 1845
KIBB4571.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 264 782 1845
Size: 264 518 1063
Fragments arranged by size:
1063 518 264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 964 1845
Size: 385 579 881
Fragments arranged by size:
881 579 385
AflIII A'CryG_T
Cuts at: 0 962 1845
Size: 962 883
ApoI r'AATT_y
Cuts at: 0 13 77 454 723 1512 1845
Size: 13 64 377 269 789 333
Fragments arranged by size:
789 377 333 269 64 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 876 1845
Size: 709 167 969
Fragments arranged by size:
969 709 167
BfrBI ATG'CAT
Cuts at: 0 1528 1845
Size: 1528 317
Bme1580I G_kGCm'C
Cuts at: 0 282 1820 1845
Size: 282 1538 25
Fragments arranged by size:
1538 282 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 707 1538 1845
Size: 707 831 307
Fragments arranged by size:
831 707 307
Bpu10I CC'TnA_GC
Cuts at: 0 407 1845
Size: 407 1438
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 859 1038 1845
Size: 859 179 807
Fragments arranged by size:
859 807 179
BsaAI yAC'GTr
Cuts at: 0 963 1845
Size: 963 882
BsaHI Gr'CG_yC
Cuts at: 0 222 1845
Size: 222 1623
BsaWI w'CCGG_w
Cuts at: 0 1532 1845
Size: 1532 313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 1367 1397 1845
Size: 82 30 1255 30 448
Fragments arranged by size:
1255 448 82 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 644 722 1845
Size: 644 78 1123
Fragments arranged by size:
1123 644 78
BseYI C'CCAG_C
Cuts at: 0 101 1845
Size: 101 1744
BsiEI CG_ry'CG
Cuts at: 0 264 1845
Size: 264 1581
BsmI GAATG_Cn'
Cuts at: 0 1528 1845
Size: 1528 317
Bsp1286I G_dGCh'C
Cuts at: 0 282 1820 1845
Size: 282 1538 25
Fragments arranged by size:
1538 282 25
BspEI T'CCGG_A
Cuts at: 0 1532 1845
Size: 1532 313
BsrGI T'GTAC_A
Cuts at: 0 965 1845
Size: 965 880
BssSI C'ACGA_G
Cuts at: 0 1754 1845
Size: 1754 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1644 1845
Size: 1644 201
BstYI r'GATC_y
Cuts at: 0 708 1845
Size: 708 1137
BtgI C'CryG_G
Cuts at: 0 1331 1845
Size: 1331 514
BtsI GCAGTG_nn'
Cuts at: 0 584 1845
Size: 584 1261
DraI TTT'AAA
Cuts at: 0 1568 1845
Size: 1568 277
EaeI y'GGCC_r
Cuts at: 0 995 1110 1253 1450 1845
Size: 995 115 143 197 395
Fragments arranged by size:
995 395 197 143 115
EarI CTCTTCn'nnn_
Cuts at: 0 507 682 943 1845
Size: 507 175 261 902
Fragments arranged by size:
902 507 261 175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 964 1845
Size: 385 579 881
Fragments arranged by size:
881 579 385
HaeII r_GCGC'y
Cuts at: 0 1054 1845
Size: 1054 791
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 506 538 1011 1043 1845
Size: 82 32 392 32 473 32 802
Fragments arranged by size:
802 473 392 82 32 32 32
HincII GTy'rAC
Cuts at: 0 265 1325 1845
Size: 265 1060 520
Fragments arranged by size:
1060 520 265
HindIII A'AGCT_T
Cuts at: 0 1555 1845
Size: 1555 290
MfeI C'AATT_G
Cuts at: 0 827 1845
Size: 827 1018
MscI TGG'CCA
Cuts at: 0 1255 1452 1845
Size: 1255 197 393
Fragments arranged by size:
1255 393 197
MslI CAynn'nnrTG
Cuts at: 0 1436 1845
Size: 1436 409
MspA1I CmG'CkG
Cuts at: 0 357 1845
Size: 357 1488
NcoI C'CATG_G
Cuts at: 0 1331 1845
Size: 1331 514
NsiI A_TGCA'T
Cuts at: 0 1530 1845
Size: 1530 315
NspI r_CATG'y
Cuts at: 0 1038 1845
Size: 1038 807
PstI C_TGCA'G
Cuts at: 0 921 1845
Size: 921 924
SalI G'TCGA_C
Cuts at: 0 263 1845
Size: 263 1582
ScaI AGT'ACT
Cuts at: 0 1804 1845
Size: 1804 41
SexAI A'CCwGG_T
Cuts at: 0 636 1845
Size: 636 1209
SfcI C'TryA_G
Cuts at: 0 917 1845
Size: 917 928
SmlI C'TyrA_G
Cuts at: 0 874 1017 1845
Size: 874 143 828
Fragments arranged by size:
874 828 143
StyI C'CwwG_G
Cuts at: 0 1250 1331 1664 1845
Size: 1250 81 333 181
Fragments arranged by size:
1250 333 181 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1176 1636 1845
Size: 1176 460 209
Fragments arranged by size:
1176 460 209
TatI w'GTAC_w
Cuts at: 0 965 1802 1845
Size: 965 837 43
Fragments arranged by size:
965 837 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1367 1399 1845
Size: 1367 32 446
Fragments arranged by size:
1367 446 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1351 1845
Size: 1351 494
Enzymes that do cut:
AccI AcuI AflIII ApoI BbsI BfrBI Bme1580I BmrI
Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsiEI BsmI Bsp1286I BspEI BsrGI BssSI BstXI BstYI
BtgI BtsI DraI EaeI EarI Eco57MI HaeII Hin4I
HincII HindIII MfeI MscI MslI MspA1I NcoI NsiI
NspI PstI SalI ScaI SexAI SfcI SmlI StyI
TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BglI BglII BlpI BmgBI
BmtI BplI BpmI BsaI BsaBI BsgI BsiHKAI BsiWI
BsmBI BspHI BspMI BsrBI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI KpnI MluI
MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI XmnI ZraI