Restriction map for
pF1KB4573
(Linear) (Six-base) MAPSORT of: KIBB4573.seq Check: 163 from: 1 to: 1944
KIBB4573.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1304 1944
Size: 1304 640
AccI GT'mk_AC
Cuts at: 0 769 1139 1592 1944
Size: 769 370 453 352
Fragments arranged by size:
769 453 370 352
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 761 854 1152 1944
Size: 460 301 93 298 792
Fragments arranged by size:
792 460 301 298 93
AfeI AGC'GCT
Cuts at: 0 1857 1944
Size: 1857 87
AhdI GACnn_n'nnGTC
Cuts at: 0 641 1944
Size: 641 1303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 823 1944
Size: 823 1121
AlwNI CAG_nnn'CTG
Cuts at: 0 964 1806 1944
Size: 964 842 138
Fragments arranged by size:
964 842 138
ApoI r'AATT_y
Cuts at: 0 469 511 591 1944
Size: 469 42 80 1353
Fragments arranged by size:
1353 469 80 42
AvaI C'yCGr_G
Cuts at: 0 1926 1944
Size: 1926 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 521 554 1944
Size: 521 33 1390
Fragments arranged by size:
1390 521 33
BanI G'GyrC_C
Cuts at: 0 954 1944
Size: 954 990
BanII G_rGCy'C
Cuts at: 0 904 1944
Size: 904 1040
BbsI GAAGACnn'nnnn_
Cuts at: 0 31 436 1802 1932 1944
Size: 31 405 1366 130 12
Fragments arranged by size:
1366 405 130 31 12
BglI GCCn_nnn'nGGC
Cuts at: 0 908 1944
Size: 908 1036
BglII A'GATC_T
Cuts at: 0 1704 1944
Size: 1704 240
Bme1580I G_kGCm'C
Cuts at: 0 957 1944
Size: 957 987
BmgBI CAC'GTC
Cuts at: 0 1922 1944
Size: 1922 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 1257 1944
Size: 1257 687
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1511 1944
Size: 1511 433
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 579 711 1506 1944
Size: 57 522 132 795 438
Fragments arranged by size:
795 522 438 132 57
Bpu10I CC'TnA_GC
Cuts at: 0 1358 1944
Size: 1358 586
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 1944
Size: 241 1703
BsaI GGTCTCn'nnnn_
Cuts at: 0 1303 1944
Size: 1303 641
BsaWI w'CCGG_w
Cuts at: 0 1850 1944
Size: 1850 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1300 1330 1944
Size: 1300 30 614
Fragments arranged by size:
1300 614 30
BseYI C'CCAG_C
Cuts at: 0 618 1944
Size: 618 1326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1944
Size: 308 1636
BsiHKAI G_wGCw'C
Cuts at: 0 8 524 1944
Size: 8 516 1420
Fragments arranged by size:
1420 516 8
BsmI GAATG_Cn'
Cuts at: 0 1559 1944
Size: 1559 385
Bsp1286I G_dGCh'C
Cuts at: 0 8 524 904 957 1944
Size: 8 516 380 53 987
Fragments arranged by size:
987 516 380 53 8
BspHI T'CATG_A
Cuts at: 0 516 1944
Size: 516 1428
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1304 1491 1944
Size: 1304 187 453
Fragments arranged by size:
1304 453 187
BsrBI CCG'CTC
Cuts at: 0 951 1944
Size: 951 993
BsrDI GCAATG_nn'
Cuts at: 0 42 1340 1745 1944
Size: 42 1298 405 199
Fragments arranged by size:
1298 405 199 42
BssSI C'ACGA_G
Cuts at: 0 995 1944
Size: 995 949
BstBI TT'CG_AA
Cuts at: 0 843 1944
Size: 843 1101
BstXI CCAn_nnnn'nTGG
Cuts at: 0 911 1944
Size: 911 1033
BstYI r'GATC_y
Cuts at: 0 147 1124 1704 1802 1944
Size: 147 977 580 98 142
Fragments arranged by size:
977 580 147 142 98
BtsI GCAGTG_nn'
Cuts at: 0 296 1944
Size: 296 1648
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1044 1079 1944
Size: 1044 35 865
Fragments arranged by size:
1044 865 35
EarI CTCTTCn'nnn_
Cuts at: 0 1738 1775 1944
Size: 1738 37 169
Fragments arranged by size:
1738 169 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 460 579 711 761 854 1152 1506
Size: 57 403 119 132 50 93 298 354
Cuts at: 1506 1944
Size: 438
Fragments arranged by size:
438 403 354 298 132 119 93 57
50
EcoO109I rG'GnC_Cy
Cuts at: 0 267 968 1944
Size: 267 701 976
Fragments arranged by size:
976 701 267
EcoRV GAT'ATC
Cuts at: 0 1899 1944
Size: 1899 45
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 465 1944
Size: 465 1479
FspI TGC'GCA
Cuts at: 0 1192 1944
Size: 1192 752
HaeII r_GCGC'y
Cuts at: 0 120 1859 1944
Size: 120 1739 85
Fragments arranged by size:
1739 120 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 627 659 1393 1425 1579 1611
Size: 340 32 255 32 734 32 154 32
Cuts at: 1611 1652 1684 1944
Size: 41 32 260
Fragments arranged by size:
734 340 260 255 154 41 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 770 1140 1944
Size: 770 370 804
Fragments arranged by size:
804 770 370
HindIII A'AGCT_T
Cuts at: 0 442 1944
Size: 442 1502
MfeI C'AATT_G
Cuts at: 0 1253 1944
Size: 1253 691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1436 1565 1851 1944
Size: 1436 129 286 93
Fragments arranged by size:
1436 286 129 93
MslI CAynn'nnrTG
Cuts at: 0 1109 1298 1675 1944
Size: 1109 189 377 269
Fragments arranged by size:
1109 377 269 189
MspA1I CmG'CkG
Cuts at: 0 77 1944
Size: 77 1867
NspI r_CATG'y
Cuts at: 0 1207 1303 1381 1944
Size: 1207 96 78 563
Fragments arranged by size:
1207 563 96 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 964 1944
Size: 964 980
PfoI T'CCnGG_A
Cuts at: 0 389 664 1304 1944
Size: 389 275 640 640
Fragments arranged by size:
640 640 389 275
PpuMI rG'GwC_Cy
Cuts at: 0 968 1944
Size: 968 976
PshAI GACnn'nnGTC
Cuts at: 0 461 1944
Size: 461 1483
PsiI TTA'TAA
Cuts at: 0 1091 1944
Size: 1091 853
PvuII CAG'CTG
Cuts at: 0 77 1944
Size: 77 1867
SalI G'TCGA_C
Cuts at: 0 768 1138 1944
Size: 768 370 806
Fragments arranged by size:
806 768 370
SanDI GG'GwC_CC
Cuts at: 0 968 1944
Size: 968 976
SfcI C'TryA_G
Cuts at: 0 525 1944
Size: 525 1419
SmlI C'TyrA_G
Cuts at: 0 256 1944
Size: 256 1688
SphI G_CATG'C
Cuts at: 0 1381 1944
Size: 1381 563
StuI AGG'CCT
Cuts at: 0 993 1165 1788 1944
Size: 993 172 623 156
Fragments arranged by size:
993 623 172 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 714 746 770 802 1944
Size: 714 32 24 32 1142
Fragments arranged by size:
1142 714 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 1920 1944
Size: 1920 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 810 1944
Size: 810 1134
XmnI GAAnn'nnTTC
Cuts at: 0 374 433 1944
Size: 374 59 1511
Fragments arranged by size:
1511 374 59
Enzymes that do cut:
AarI AccI AcuI AfeI AhdI AlfI AlwNI ApoI
AvaI BaeI BanI BanII BbsI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI
BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BssSI BstBI BstXI BstYI BtsI CspCI
EarI Eco57MI EcoO109I EcoRV FalI FspI HaeII Hin4I
HincII HindIII MfeI MmeI MslI MspA1I NspI PflMI
PfoI PpuMI PshAI PsiI PvuII SalI SanDI SfcI
SmlI SphI StuI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BlpI BmtI BsaAI
BsaBI BsaHI BseRI BsiEI BsiWI BsmBI BspEI BsrFI
BsrGI BssHII BstAPI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI FseI FspAI HpaI KasI KpnI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StyI SwaI TaqII
TatI XbaI XhoI XmaI ZraI